To explore the functional mechanism of tetraarsenic tetrasulfide（As4S4） against the growth and proliferation of colon cancer cells ls174t, MTT method was used to observe the functions of tetraarsenic tetrasulfide fo...To explore the functional mechanism of tetraarsenic tetrasulfide（As4S4） against the growth and proliferation of colon cancer cells ls174t, MTT method was used to observe the functions of tetraarsenic tetrasulfide for the inhibition of cells ls174t in vitro. Transmission electron microscope was used to observe cell morphologies. FCM assay was performed to measure the change of cell cycle. RT-PCR method was used to detect the expressions of bcl-2 and bax mRNA. Westernblot method was used to detect the expressions of bcl-2 and bax protein. Tetraarsenic tetra- sulfide showed an inhibiting effect on the proliferation of cells ls174t in vitro（P〈0.01）. It induces the apoptosis of cells ls174t, which is related to the decrease in the expression of bcl-2 and the increase in the expression of bax.展开更多
基金Supported by Key Project of Heilongjiang Commission of Science and Technology, China(No.GB03C601-3).
文摘To explore the functional mechanism of tetraarsenic tetrasulfide（As4S4） against the growth and proliferation of colon cancer cells ls174t, MTT method was used to observe the functions of tetraarsenic tetrasulfide for the inhibition of cells ls174t in vitro. Transmission electron microscope was used to observe cell morphologies. FCM assay was performed to measure the change of cell cycle. RT-PCR method was used to detect the expressions of bcl-2 and bax mRNA. Westernblot method was used to detect the expressions of bcl-2 and bax protein. Tetraarsenic tetra- sulfide showed an inhibiting effect on the proliferation of cells ls174t in vitro（P〈0.01）. It induces the apoptosis of cells ls174t, which is related to the decrease in the expression of bcl-2 and the increase in the expression of bax.
