Induction of the activity of ethoxyresorufin-o-deethylase(EROD),one of the isozymes ofmixed function oxidase,in human squamouscarcinoma cells of the tongue(SCC-15G andSCC-25)treated with TCDD was invistigated.
This study explored that the mechanism aboutTCDD-induced cellular proliferation with themonolayer growth of cells,SCC-15G and SCC-25 and synthesis using incorporation of ~3H-TdR into DNA.It is indicated that TCDD-in-d...This study explored that the mechanism aboutTCDD-induced cellular proliferation with themonolayer growth of cells,SCC-15G and SCC-25 and synthesis using incorporation of ~3H-TdR into DNA.It is indicated that TCDD-in-duced increase in cell number and DNA synth-展开更多
The 84 km Panama Canal connecting the Atlantic and Pacific oceans has historically been a strategic waterway for shipping and the location of United States (US) military bases. Since the construction of Lake Gatun res...The 84 km Panama Canal connecting the Atlantic and Pacific oceans has historically been a strategic waterway for shipping and the location of United States (US) military bases. Since the construction of Lake Gatun reservoir, canal locks and navigation channel through the Isthmus of Panama tropical forests in the 1910s, chemicals, pesticides, and herbicides have been essential for controlling upland and wetland vegetation as well as managing mosquito-borne diseases. Chemicals and pesticides flowed into Lake Gatun via land surface runoff and subsurface drainage either attached to the sediment or in solution during the rainy season. Lake Gatun and the Panama Canal was the drinking water source for most of the civilian and military population living in the Panama Canal Zone. Between 1948 and 1999, US military base commanders had the ability to order, from the Federal Supply Catalog, commercially available herbicide 2,4,5-T with unknown amounts of dioxin TCDD for use on the military base grounds in the Panama Canal Zone. The herbicide 2,4,5-T was transported to Panama Canal Zone ports, including the ports Cristobal on the Caribbean and Balboa on the Pacific, and distributed to the US military bases in Panama by rail or truck. The US National Toxicology Program and the International Agency for the Research on Cancer listed dioxin and TCDD as known human carcinogens. Dioxins are endocrine disrupters and can cause certain chloracne, cancers, developmental and reproductive effects. In 1985, the United States government banned the manufacture of the herbicide 2,4,5-T, with unknown amounts of dioxin TCDD, after it was shown to cause cancer in animals. The objectives of this study are to determine: 1) the fate of dioxin TCDD, a contaminant in the herbicide 2,4,5-T, sprayed on the US military base vegetation in the Panama Canal Zone from 1948 to 1999, 2) the transport of dioxin TCDD rich sediment via soil erosion and overland flow into Lake Gatun and Panama Canal waterways and 3) the human health impacts of dioxin TCDD, a known carcinogen, on US military and Panamanian civilians exposed to dioxin TCDD in the Panama Canal Zone.展开更多
研究运动对2,3,7,8-四氯二苯并二噁英(2,3,7,8-TCDD)持续染毒大鼠肝脏脂质合成代谢关键酶乙酰辅酶A羧化酶1(ACC1)、脂肪酸合成酶(FAS)、硬脂酰辅酶A去饱和酶1(SCD1)m RNA及转录因子肝X受体a(LXRa)蛋白表达的影响,探讨环境污染物引发代...研究运动对2,3,7,8-四氯二苯并二噁英(2,3,7,8-TCDD)持续染毒大鼠肝脏脂质合成代谢关键酶乙酰辅酶A羧化酶1(ACC1)、脂肪酸合成酶(FAS)、硬脂酰辅酶A去饱和酶1(SCD1)m RNA及转录因子肝X受体a(LXRa)蛋白表达的影响,探讨环境污染物引发代谢性疾病的发病机制,为运动锻炼防控环境健康风险提供理论支持。将24只8周龄雄性大鼠随机分为对照组(C组)、染毒组(T组)、运动染毒组(ET组)。T、ET组腹腔注射首剂量6.4μg·kg-1(以单位体重计)的2,3,7,8-TCDD,之后每隔1周给予上述剂量的21%持续染毒,连续7周。ET组尾部负重(5%体重)进行游泳运动,每周5 d,每次30 min。8周后处死动物,计算肝脏相对重量,检测肝脏甘油三酯(TG)含量,实时荧光定量PCR检测肝脏ACC1、FAS、SCD1 m RNA表达,免疫印迹法(Western Blot)检测肝脏LXRa蛋白表达。结果显示8周2,3,7,8-TCDD持续染毒可显著增加肝脏脂质合成代谢关键酶ACC1、FAS、SCD1 m RNA及转录因子LXRa蛋白表达,8周游泳运动可显著降低染毒大鼠ACC1、FAS、SCD1 m RNA及LXRa蛋白表达。上述结果表明2,3,7,8-TCDD可以引起大鼠肝脏LXRa蛋白表达增高,进而LXRa通过调控靶基因ACC1、FAS、SCD1 m RNA的表达,造成脂质代谢紊乱,肝脏甘油三酯沉积,而有氧运动降低了肝脏中脂质的沉积,提示运动干预可以改善二噁英类污染物造成的肝脏脂质代谢紊乱。展开更多
文摘Induction of the activity of ethoxyresorufin-o-deethylase(EROD),one of the isozymes ofmixed function oxidase,in human squamouscarcinoma cells of the tongue(SCC-15G andSCC-25)treated with TCDD was invistigated.
文摘This study explored that the mechanism aboutTCDD-induced cellular proliferation with themonolayer growth of cells,SCC-15G and SCC-25 and synthesis using incorporation of ~3H-TdR into DNA.It is indicated that TCDD-in-duced increase in cell number and DNA synth-
文摘The 84 km Panama Canal connecting the Atlantic and Pacific oceans has historically been a strategic waterway for shipping and the location of United States (US) military bases. Since the construction of Lake Gatun reservoir, canal locks and navigation channel through the Isthmus of Panama tropical forests in the 1910s, chemicals, pesticides, and herbicides have been essential for controlling upland and wetland vegetation as well as managing mosquito-borne diseases. Chemicals and pesticides flowed into Lake Gatun via land surface runoff and subsurface drainage either attached to the sediment or in solution during the rainy season. Lake Gatun and the Panama Canal was the drinking water source for most of the civilian and military population living in the Panama Canal Zone. Between 1948 and 1999, US military base commanders had the ability to order, from the Federal Supply Catalog, commercially available herbicide 2,4,5-T with unknown amounts of dioxin TCDD for use on the military base grounds in the Panama Canal Zone. The herbicide 2,4,5-T was transported to Panama Canal Zone ports, including the ports Cristobal on the Caribbean and Balboa on the Pacific, and distributed to the US military bases in Panama by rail or truck. The US National Toxicology Program and the International Agency for the Research on Cancer listed dioxin and TCDD as known human carcinogens. Dioxins are endocrine disrupters and can cause certain chloracne, cancers, developmental and reproductive effects. In 1985, the United States government banned the manufacture of the herbicide 2,4,5-T, with unknown amounts of dioxin TCDD, after it was shown to cause cancer in animals. The objectives of this study are to determine: 1) the fate of dioxin TCDD, a contaminant in the herbicide 2,4,5-T, sprayed on the US military base vegetation in the Panama Canal Zone from 1948 to 1999, 2) the transport of dioxin TCDD rich sediment via soil erosion and overland flow into Lake Gatun and Panama Canal waterways and 3) the human health impacts of dioxin TCDD, a known carcinogen, on US military and Panamanian civilians exposed to dioxin TCDD in the Panama Canal Zone.
文摘研究运动对2,3,7,8-四氯二苯并二噁英(2,3,7,8-TCDD)持续染毒大鼠肝脏脂质合成代谢关键酶乙酰辅酶A羧化酶1(ACC1)、脂肪酸合成酶(FAS)、硬脂酰辅酶A去饱和酶1(SCD1)m RNA及转录因子肝X受体a(LXRa)蛋白表达的影响,探讨环境污染物引发代谢性疾病的发病机制,为运动锻炼防控环境健康风险提供理论支持。将24只8周龄雄性大鼠随机分为对照组(C组)、染毒组(T组)、运动染毒组(ET组)。T、ET组腹腔注射首剂量6.4μg·kg-1(以单位体重计)的2,3,7,8-TCDD,之后每隔1周给予上述剂量的21%持续染毒,连续7周。ET组尾部负重(5%体重)进行游泳运动,每周5 d,每次30 min。8周后处死动物,计算肝脏相对重量,检测肝脏甘油三酯(TG)含量,实时荧光定量PCR检测肝脏ACC1、FAS、SCD1 m RNA表达,免疫印迹法(Western Blot)检测肝脏LXRa蛋白表达。结果显示8周2,3,7,8-TCDD持续染毒可显著增加肝脏脂质合成代谢关键酶ACC1、FAS、SCD1 m RNA及转录因子LXRa蛋白表达,8周游泳运动可显著降低染毒大鼠ACC1、FAS、SCD1 m RNA及LXRa蛋白表达。上述结果表明2,3,7,8-TCDD可以引起大鼠肝脏LXRa蛋白表达增高,进而LXRa通过调控靶基因ACC1、FAS、SCD1 m RNA的表达,造成脂质代谢紊乱,肝脏甘油三酯沉积,而有氧运动降低了肝脏中脂质的沉积,提示运动干预可以改善二噁英类污染物造成的肝脏脂质代谢紊乱。
