Black locust (Robinia pseudoacacia L.) was the first forest tree species to be imported from North America to Europe at the beginning of the 17th century. It is the most important fast-growing stand-forming tree speci...Black locust (Robinia pseudoacacia L.) was the first forest tree species to be imported from North America to Europe at the beginning of the 17th century. It is the most important fast-growing stand-forming tree species in Hungary. Black locust planta-tions can be successfully established in response to a range of economic and ecological opportunities. Plantation survival and productivity are maximized by matching the species' growth characteristics with silvicultural options and land management needs. In the paper the sequence of forest tending operations in black locust stands is proposed, based on results of long-term stand structure and forest yield trials. Implementing good silvicultural plans and models will lead to profitable black locust stands and greater ac-ceptance of the species by land managers. Black locust would also be a very useful species for energy productions as the related research results have been shown in the paper.展开更多
Black locust (Robinia pseudoacacia L.) is one of the most important stand-forming tree species in Hungary and its importance is increasing in many countries. Black locust plants are commonly produced by two methods, b...Black locust (Robinia pseudoacacia L.) is one of the most important stand-forming tree species in Hungary and its importance is increasing in many countries. Black locust plants are commonly produced by two methods, by seed and by root cuttings. Tissue culture propagation can be considered as a relatively new method. Growing trees from seed is a relatively sim-ple method for reliably producing seedlings on a large scale under a variety of circumstances. Mechanization of the method is easy and the production cost is relatively low. Propagation from root cuttings and tissue culture are valuable for reproduction of superior individuals or varieties. By applying these methods, superior traits of the selected trees can be preserved in the clones. Recent experiments demonstrated that micropropagated trees could be successfully transplanted into soil, hardened and grown in the field.展开更多
The effectiveness of thidiazuron（TDZ）on adventitious bud and shoot formation from leaves of Robinia pseudoacacia was tested in combination with naphthaleneacetic acid （NAA）or 2,4-dichiorophenoxyacetic acid （2,4-D...The effectiveness of thidiazuron（TDZ）on adventitious bud and shoot formation from leaves of Robinia pseudoacacia was tested in combination with naphthaleneacetic acid （NAA）or 2,4-dichiorophenoxyacetic acid （2,4-D）,With the increasing of TDZ level,the induction rate of adventitious buds appeared an increasing trend and the highest rate was obtained by the combination of 2mg/L TDZ whth 0.1mg/L NAA.The excessive level of TDZ could induce useless cellae and easily produce hyperhydricity.Inhibition of shoot elongation by TDZ was overcome through transferring shoot cultures to a shoot proliferation medium lacking TDZ orcontaining NAA,benzyladenine （BA）and TDZ.Indole-3-butyric acid （IBA）was used to stimulate the rooting in vitro .Based on the number and length of rooting ,the treatment with 0.5mg/L IBA was the best.展开更多
Differences in drought tolerance were studied among three strains of black locust(Robinia pseudoacacia L.) clones(one-year-old micropropagated K2,K3 clones and a common diploid clone CK) under simulated natural drough...Differences in drought tolerance were studied among three strains of black locust(Robinia pseudoacacia L.) clones(one-year-old micropropagated K2,K3 clones and a common diploid clone CK) under simulated natural drought conditions.The experiment was carried out as a randomized complete blocks design.The following physiological variables were examined,biomass and allocation,photosynthetic characteristics,i.e.net photosynthetic rate(Pn),transpiration rate(E),stomatal conductance(Gs),intercellular CO2 concentration(Ci),instantaneous water use efficiency(WUEI),and long-term water use efficiency(WUEL).Results showed that the K2 and K3 had greater biomass,with the K3 displaying favorable ratio of root biomass to shoot and leaf biomass.Net photosynthetic rate(Pn) declined along with Gs,Ci and the ratio intercellular/ambient(Ci/Ca),under increasing drought stress.The decline in photosynthesis was mainly due to stomatal limitation.The K2 and K3 showed higher WUE and Pn than the CK.There were significant differences of drought level on WUEL,and the WUEL of K2 and K3 was greater than that of CK.Evaluation based overall physiological characteristics indicates the K2 and K3 are more drought tolerant,which are well adapted to the drought-stricken area of Northwest China.展开更多
The tissue culture conditions of fast-growing clone K2 and fodder-type clone K3 of Robinia pseudoacacia L. tetraploid were investigated using shoot sprouting in hydroponic growth chamber as explants. After disinfected...The tissue culture conditions of fast-growing clone K2 and fodder-type clone K3 of Robinia pseudoacacia L. tetraploid were investigated using shoot sprouting in hydroponic growth chamber as explants. After disinfected by 70% ethanol then sterilized with NaClO solution containing 0.5% available chlorine,the explants should be initially cultured with MS medium (containing 30 g·L-1 sucrose and 6 g·L-1 agar,pH 5.8),and survival rate of K2 and K3 explants all reaches 90% in initial culture medium. The best proliferation medium of K2 adventitious bud is MS medium adding 0.3 mg·L-1 6-BA and 0.05 mg·L-1 NAA,with the highest proliferation coefficient of 5.29,and that of K3 is MS medium adding 1.0 mg·L-1 6-BA and 0.10 mg·L-1 NAA,with the highest proliferation coefficient of 3.36. The optimized rooting medium of K2 and K3 plantlets is 1/2MS medium (containing 30 g·L-1 sucrose and 6 g·L-1 agar,pH 5.8) adding 0.1 mg·L-1 IBA and 0.1 mg·L-1 NAA,respectively. After training,K2 and K3 plantlets are transplanted by two-step transplanting method,all of survival rate reach 98%.展开更多
文摘Black locust (Robinia pseudoacacia L.) was the first forest tree species to be imported from North America to Europe at the beginning of the 17th century. It is the most important fast-growing stand-forming tree species in Hungary. Black locust planta-tions can be successfully established in response to a range of economic and ecological opportunities. Plantation survival and productivity are maximized by matching the species' growth characteristics with silvicultural options and land management needs. In the paper the sequence of forest tending operations in black locust stands is proposed, based on results of long-term stand structure and forest yield trials. Implementing good silvicultural plans and models will lead to profitable black locust stands and greater ac-ceptance of the species by land managers. Black locust would also be a very useful species for energy productions as the related research results have been shown in the paper.
基金the Hungarian National Sci-entific Research Fund (OTKA-T 029021) and the Hungar- ian Ministry of Agriculture and Region Development (95-a/2001).
文摘Black locust (Robinia pseudoacacia L.) is one of the most important stand-forming tree species in Hungary and its importance is increasing in many countries. Black locust plants are commonly produced by two methods, by seed and by root cuttings. Tissue culture propagation can be considered as a relatively new method. Growing trees from seed is a relatively sim-ple method for reliably producing seedlings on a large scale under a variety of circumstances. Mechanization of the method is easy and the production cost is relatively low. Propagation from root cuttings and tissue culture are valuable for reproduction of superior individuals or varieties. By applying these methods, superior traits of the selected trees can be preserved in the clones. Recent experiments demonstrated that micropropagated trees could be successfully transplanted into soil, hardened and grown in the field.
基金Supported by project of the Science and technology Ministry of China (Grant No.02 EFN216700794)
文摘The effectiveness of thidiazuron（TDZ）on adventitious bud and shoot formation from leaves of Robinia pseudoacacia was tested in combination with naphthaleneacetic acid （NAA）or 2,4-dichiorophenoxyacetic acid （2,4-D）,With the increasing of TDZ level,the induction rate of adventitious buds appeared an increasing trend and the highest rate was obtained by the combination of 2mg/L TDZ whth 0.1mg/L NAA.The excessive level of TDZ could induce useless cellae and easily produce hyperhydricity.Inhibition of shoot elongation by TDZ was overcome through transferring shoot cultures to a shoot proliferation medium lacking TDZ orcontaining NAA,benzyladenine （BA）and TDZ.Indole-3-butyric acid （IBA）was used to stimulate the rooting in vitro .Based on the number and length of rooting ,the treatment with 0.5mg/L IBA was the best.
文摘Differences in drought tolerance were studied among three strains of black locust(Robinia pseudoacacia L.) clones(one-year-old micropropagated K2,K3 clones and a common diploid clone CK) under simulated natural drought conditions.The experiment was carried out as a randomized complete blocks design.The following physiological variables were examined,biomass and allocation,photosynthetic characteristics,i.e.net photosynthetic rate(Pn),transpiration rate(E),stomatal conductance(Gs),intercellular CO2 concentration(Ci),instantaneous water use efficiency(WUEI),and long-term water use efficiency(WUEL).Results showed that the K2 and K3 had greater biomass,with the K3 displaying favorable ratio of root biomass to shoot and leaf biomass.Net photosynthetic rate(Pn) declined along with Gs,Ci and the ratio intercellular/ambient(Ci/Ca),under increasing drought stress.The decline in photosynthesis was mainly due to stomatal limitation.The K2 and K3 showed higher WUE and Pn than the CK.There were significant differences of drought level on WUEL,and the WUEL of K2 and K3 was greater than that of CK.Evaluation based overall physiological characteristics indicates the K2 and K3 are more drought tolerant,which are well adapted to the drought-stricken area of Northwest China.
文摘The tissue culture conditions of fast-growing clone K2 and fodder-type clone K3 of Robinia pseudoacacia L. tetraploid were investigated using shoot sprouting in hydroponic growth chamber as explants. After disinfected by 70% ethanol then sterilized with NaClO solution containing 0.5% available chlorine,the explants should be initially cultured with MS medium (containing 30 g·L-1 sucrose and 6 g·L-1 agar,pH 5.8),and survival rate of K2 and K3 explants all reaches 90% in initial culture medium. The best proliferation medium of K2 adventitious bud is MS medium adding 0.3 mg·L-1 6-BA and 0.05 mg·L-1 NAA,with the highest proliferation coefficient of 5.29,and that of K3 is MS medium adding 1.0 mg·L-1 6-BA and 0.10 mg·L-1 NAA,with the highest proliferation coefficient of 3.36. The optimized rooting medium of K2 and K3 plantlets is 1/2MS medium (containing 30 g·L-1 sucrose and 6 g·L-1 agar,pH 5.8) adding 0.1 mg·L-1 IBA and 0.1 mg·L-1 NAA,respectively. After training,K2 and K3 plantlets are transplanted by two-step transplanting method,all of survival rate reach 98%.
