Toxicity assessment and screening of tetrodotoxin in the oblong blowfish(Takifugu oblongus)from the Tamil Nadu Coast of Bay of Bengal,India

Objective:To assess the toxicity of the pufferfish Takifugu oblongus,from Chennai coast,Tamil Nadu,India and to detect the presence of Tetrodotoxin(TTX).Methods:The toxicity was evaluated by mouse bioassay using Swiss Albino mice which were expressed in mouse units(MU).Gross anatomical features were observed which is followed by histopathology of the dead mice tissues to establish the toxicity.Instrumental analysis for the presence of tetrodotoxin was also performed through GC-MS and HPLC.Results:The toxicity of ovary was the maximum with 163 MU/g and lowest toxicity was observed in skin with 75.88 MU/g.Histopathological analyses of the dead mice showed various cellular degenerations and inflammations.The amount of Tetrodotoxin detected through GC-MS and HPLC was more reliable and sensitive than the customary mouse bioassay as instrumental analyses were able to detect even nanograms of the toxin.Conclusions:The present study evidently proved that Takifugu oblongus is highly toxic and consumption of the same can pose serious threat for health and possible lethality to humans.

河豚鱼的去毒方法与去毒后的风味调整

对江苏省餐饮业常见的私下加工的几种河豚鱼品种的毒性进行检测 ,并用自配的 2种碱性去毒剂和1种非碱性去毒剂对河豚鱼的毒素进行去毒方法的比较和筛选试验 ,同时还对去毒后鱼体风味的调整进行了试验研究 。

宁波地区织纹螺毒性特征探索研究

目的了解宁波地区有毒织纹螺毒素的类型、分布、消长及毒素来源。方法小鼠生物法测定织纹螺毒性;ELISA法及HPLC等检测织纹螺石房蛤毒素(STX)、膝沟藻毒素(GTX)、河豚鱼毒素(TTX)。结果从长期监测点采集的127份织纹螺样品中检出毒螺63份,检出率49.6%,样品的毒性高峰和低谷分别出现在1991年和1988年。不同栖息地毒螺检出率差异有统计学意义(P<0.01)。3类不同来源样品毒螺检出率及毒性差异也有统计学意义(P<0.05)。半褶织纹螺、红带织纹螺、正织纹螺带毒机率较高,且毒性强。检出织纹螺STX、GTX和TTX毒素。结论宁波地区织纹螺毒素由TTX或贝类麻痹性毒素(PSP)中的STX和GTX组成,部分样品TTX和STX两种毒素共存。查清了宁波市织纹螺带毒情况、地域分布及织纹螺品种与毒性相关性。监测结果提示长期以来宁波市织纹螺总体保持一定的毒性,食用易引起中毒事件的发生。