Objective To investigate the relations between neuroapoptosis and the onset and development of Alzheimer’s disease （AD）, especially the role of NF-κB in the regulation of neuroapoptosis. Methods Caspase-3 and NF-...Objective To investigate the relations between neuroapoptosis and the onset and development of Alzheimer’s disease （AD）, especially the role of NF-κB in the regulation of neuroapoptosis. Methods Caspase-3 and NF-κB （p50） expressions in the CA3 region of the hippocampus in APPswe Tg2576 transgenic mice were studied from postnatal day 0-180, using Nissl staining, immunohistochemistry and RT-PCR methods. Results Both neuronal apoptosis and NF-κB activity decreased gradually with the increase of age in wild type and Tg2576 mice. However, the number of caspase-3-positive or NF- κB-positive pyramidal cells in Tg2576 mice was greater than that in age-matched wild type mice, with significant differences after postnatal day 14 （P 0.01 or P 0.05）. Linear regression analyses of caspase-3 and NF-κB expression demonstrated a correlation between neuroapoptosis and activity of NF-κB. Conclusion The process of neuroapoptosis is consistent with the onset and development of AD. Furthermore, the observed correlation between neuroapoptosis and NF-κB activity suggests a role of NF-κB in hippocampal neuroapoptosis.展开更多
基金supported by theNational Natural Science Foundation of China (No. 30670688,30771140)the Natural Science Foundation of Education De-partment of Henan Province (No. 2007180008)+1 种基金the Natural Science Key Program of Henan University (No. 2008YBGG048)the International Cooperation Program of Science andTechnique Department of Henan Province, China (No.094300510044)
文摘Objective To investigate the relations between neuroapoptosis and the onset and development of Alzheimer’s disease （AD）, especially the role of NF-κB in the regulation of neuroapoptosis. Methods Caspase-3 and NF-κB （p50） expressions in the CA3 region of the hippocampus in APPswe Tg2576 transgenic mice were studied from postnatal day 0-180, using Nissl staining, immunohistochemistry and RT-PCR methods. Results Both neuronal apoptosis and NF-κB activity decreased gradually with the increase of age in wild type and Tg2576 mice. However, the number of caspase-3-positive or NF- κB-positive pyramidal cells in Tg2576 mice was greater than that in age-matched wild type mice, with significant differences after postnatal day 14 （P 0.01 or P 0.05）. Linear regression analyses of caspase-3 and NF-κB expression demonstrated a correlation between neuroapoptosis and activity of NF-κB. Conclusion The process of neuroapoptosis is consistent with the onset and development of AD. Furthermore, the observed correlation between neuroapoptosis and NF-κB activity suggests a role of NF-κB in hippocampal neuroapoptosis.
