AIM To investigate the role of regulatory T cell(Treg) subsets in the balance between Treg and T helper 17(Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.METHODS T r e g c e l l s...AIM To investigate the role of regulatory T cell(Treg) subsets in the balance between Treg and T helper 17(Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.METHODS T r e g c e l l s, T r e g c e l l s u b s e t s, T h 1 7 c e l l s, a n d CD4+CD25+FoxP 3+IL-17+ cells from the lamina propria of colon(LPC) and other ulcerative colitis(UC) mouse tissues were evaluated by flow cytometry. Forkhead box protein 3(FoxP 3), interleukin 17A(IL-17A), and RORC m RNA levels were assessed by real-time PCR, while interleukin-10(IL-10) and IL-17 A levels were detected with a Cytometric Beads Array.RESULTS In peripheral blood monocytes(PBMC), mesenteric lymphnode(MLN), lamina propria of jejunum(LPJ) and LPC from UC mice, Treg cell numbers were increased(P < 0.05), and FoxP 3 and IL-10 mR NA levels were decreased. Th17 cell numbers were also increased in PBMC and LPC, as were IL-17 A levels in PBMC, LPJ, and serum. The number of FrI subset cells(CD4+CD45RA+FoxP 3low) was increased in the spleen, MLN, LPJ, and LPC. FrI I subset cells(CD4+CD45RA-Fox P3high) were decreased among PBMC, MLN, LPJ, and LPC, but the number of Fr III cells(CD4+CD45RA-FoxP 3low) and CD4+CD25+FoxP 3+IL-17A+ cells was increased. Fox P3 m RNA levels in CD4+CD45RA-Fox P3 low cells decreased in PBMC, MLN, LPJ, and LPC in UC mice, while IL-17 A and RORC mR NA increased. In UC mice the distribution of Treg, Th17 cells, CD4+CD45RA-FoxP 3high, and CD4+CD45RA-FoxP 3low cells was higher in LPC relative to other tissues.CONCLUSION Increased numbers of CD4+CD45RA-FoxP 3low cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the LPC rather than secondary lymphoid tissues.展开更多
目的探讨强肌健力胶囊对重症肌无力(Myasthenia gravis,MG)患者T细胞(Regulatory T cells,Treg)、淋巴细胞亚群(CD4^(+)、CD8^(+)T淋巴细胞)、白细胞介素-17(Interleukin-17,IL-17)、白细胞介素-10(Interleukin-10,IL-10)水平及疗效的...目的探讨强肌健力胶囊对重症肌无力(Myasthenia gravis,MG)患者T细胞(Regulatory T cells,Treg)、淋巴细胞亚群(CD4^(+)、CD8^(+)T淋巴细胞)、白细胞介素-17(Interleukin-17,IL-17)、白细胞介素-10(Interleukin-10,IL-10)水平及疗效的影响。方法选取2018年1月—2021年5月期间辽宁中医药大学附属医院收治的重症肌无力患者280例作为研究对象,采用随机数字表法将其分为对照组和观察组,每组140例。对照组采用糖皮质激素醋酸地塞米松片治疗,观察组在此基础上加用强肌健力胶囊。治疗3个月后,观察比较两组患者临床疗效、不良反应,治疗前后外周血Treg细胞水平、淋巴细胞亚群水平、外周血IL-17、IL-10水平。结果治疗后两组患者Treg、CD4^(+)T细胞百分率均较治疗前明显下降,CD8^(+)T细胞百分率均较治疗前明显升高,差异有统计学意义(P<0.05);且观察组Treg、CD4^(+)T细胞百分率明显低于对照组,CD8^(+)T细胞百分率明显高于对照组,差异有统计学意义(P<0.05)。治疗后两组患者IL-17、IL-10水平均较治疗前明显降低,差异有统计学意义(P<0.05);且观察组IL-17、IL-10水平明显低于对照组,差异有统计学意义(P<0.05)。治疗后观察组总有效率为94.81%,对照组为88.72%,两组比较,差异无统计学意义(P>0.05)。治疗期间,观察组血压升高患者数量低于对照组,差异有统计学意义(P<0.05),两组患者骨质疏松、消化道症状不良反应发生率比较,差异无统计学意义(P>0.05)。结论强肌健力胶囊治疗MG疗效显著,可明显改善机体免疫功能,同时不会增加不良反应发生率。展开更多
目的:观察参地颗粒对慢性肾炎脾肾亏虚型患者Th17细胞免疫功能的干预作用。方法:90例慢性肾炎患者按随机数字表法分为试验组和对照组各45例,2组均给予西医基础治疗,对照组加用缬沙坦胶囊(代文)80 mg/d,试验组在对照组基础上加用参地颗...目的:观察参地颗粒对慢性肾炎脾肾亏虚型患者Th17细胞免疫功能的干预作用。方法:90例慢性肾炎患者按随机数字表法分为试验组和对照组各45例,2组均给予西医基础治疗,对照组加用缬沙坦胶囊(代文)80 mg/d,试验组在对照组基础上加用参地颗粒,2组疗程均为12周。观察24 h尿蛋白定量(24 h Upro)、尿红细胞计数(URBC),并采用流式细胞技术检测外周血CD4^+、CD8^+和Th17细胞含量,酶联免疫吸附法检测血清白介素-17(IL-17)水平。结果:试验组疾病疗效总有效率显著高于对照组。试验组治疗后24 h Upro、URBC与治疗前比较均显著降低,且24 h Upro亦显著低于同期治疗后的对照组。试验组治疗后的Th17含量及IL-17水平与治疗前比较均有显著降低,CD4^+、CD4^+/CD8^+比值显著提升,且其CD4^+/CD8^+比值、Th17含量及IL-17水平变化与对照组比较亦差异有统计学意义。结论:参地颗粒可以通过调节Th17细胞功能,从而改善慢性肾炎脾肾亏虚型患者的免疫功能,发挥临床疗效。展开更多
AIM:To investigate the feasibility and mechanism of immune tolerance in allergic conjunctivitis.METHODS:The allergic conjunctivitis immune tolerance mice model was established by ragweed pollen(RW)and the related cyto...AIM:To investigate the feasibility and mechanism of immune tolerance in allergic conjunctivitis.METHODS:The allergic conjunctivitis immune tolerance mice model was established by ragweed pollen(RW)and the related cytokines were detected.The mice were divided into 9 groups and the maslinic acid(MA)or PBS were given for different group after modeling.The expression levels of chemokine ligand 5(CCL5)and P-65 in the conjunctival tissue were analyzed by immunohistochemistry,quantitative reverse transcription polymerase chain reaction(q RT-PCR)and Western blot.The percentage of interleukin-17(IL-17)and CD4+CD25+in the splenocyte supernatant was analyzed by flow cytometry.Fur thermore,the serum and splenocyte supernatant concentration of total-IgE,interleukin-10(IL-10),and IL-17 was analyzed by enzyme linked immune response(ELISA).RESULTS:After the model was established,symptoms of conjunctivitis were alleviated,the level of P-65,CCL5,IL-17,and total-IgE was raised,while the expression of IL-10,CD4+CD25+was decreased.This result fully demonstrated that a typical IL-17/regulatory-T-cells(Treg cells)imbalance and NF-κB activation.When the NF-κB signal pathway was suppressed,it showed that there was a further relief of conjunctivitis in mice.At the same time,the expression of total-IgE,IL-17,and CCL5 was decreased and the expression of anti-inflammatory factor(IL-10,CD4+CD25+)was increased.CONCLUSION:In the state of immune tolerance,symptoms of conjunctivitis in mice are alleviated,the Th-17 cells of allergic conjunctivitis mice are inhibited,and Treg cells activity is enhanced.展开更多
基金Supported by the National Natural Science Foundation of China,No.81300294State Scholarship Fund of China,No.201509110033
文摘AIM To investigate the role of regulatory T cell(Treg) subsets in the balance between Treg and T helper 17(Th17) cells in various tissues from mice with dextran sulfate sodium-induced colitis.METHODS T r e g c e l l s, T r e g c e l l s u b s e t s, T h 1 7 c e l l s, a n d CD4+CD25+FoxP 3+IL-17+ cells from the lamina propria of colon(LPC) and other ulcerative colitis(UC) mouse tissues were evaluated by flow cytometry. Forkhead box protein 3(FoxP 3), interleukin 17A(IL-17A), and RORC m RNA levels were assessed by real-time PCR, while interleukin-10(IL-10) and IL-17 A levels were detected with a Cytometric Beads Array.RESULTS In peripheral blood monocytes(PBMC), mesenteric lymphnode(MLN), lamina propria of jejunum(LPJ) and LPC from UC mice, Treg cell numbers were increased(P < 0.05), and FoxP 3 and IL-10 mR NA levels were decreased. Th17 cell numbers were also increased in PBMC and LPC, as were IL-17 A levels in PBMC, LPJ, and serum. The number of FrI subset cells(CD4+CD45RA+FoxP 3low) was increased in the spleen, MLN, LPJ, and LPC. FrI I subset cells(CD4+CD45RA-Fox P3high) were decreased among PBMC, MLN, LPJ, and LPC, but the number of Fr III cells(CD4+CD45RA-FoxP 3low) and CD4+CD25+FoxP 3+IL-17A+ cells was increased. Fox P3 m RNA levels in CD4+CD45RA-Fox P3 low cells decreased in PBMC, MLN, LPJ, and LPC in UC mice, while IL-17 A and RORC mR NA increased. In UC mice the distribution of Treg, Th17 cells, CD4+CD45RA-FoxP 3high, and CD4+CD45RA-FoxP 3low cells was higher in LPC relative to other tissues.CONCLUSION Increased numbers of CD4+CD45RA-FoxP 3low cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the LPC rather than secondary lymphoid tissues.
文摘目的探讨强肌健力胶囊对重症肌无力(Myasthenia gravis,MG)患者T细胞(Regulatory T cells,Treg)、淋巴细胞亚群(CD4^(+)、CD8^(+)T淋巴细胞)、白细胞介素-17(Interleukin-17,IL-17)、白细胞介素-10(Interleukin-10,IL-10)水平及疗效的影响。方法选取2018年1月—2021年5月期间辽宁中医药大学附属医院收治的重症肌无力患者280例作为研究对象,采用随机数字表法将其分为对照组和观察组,每组140例。对照组采用糖皮质激素醋酸地塞米松片治疗,观察组在此基础上加用强肌健力胶囊。治疗3个月后,观察比较两组患者临床疗效、不良反应,治疗前后外周血Treg细胞水平、淋巴细胞亚群水平、外周血IL-17、IL-10水平。结果治疗后两组患者Treg、CD4^(+)T细胞百分率均较治疗前明显下降,CD8^(+)T细胞百分率均较治疗前明显升高,差异有统计学意义(P<0.05);且观察组Treg、CD4^(+)T细胞百分率明显低于对照组,CD8^(+)T细胞百分率明显高于对照组,差异有统计学意义(P<0.05)。治疗后两组患者IL-17、IL-10水平均较治疗前明显降低,差异有统计学意义(P<0.05);且观察组IL-17、IL-10水平明显低于对照组,差异有统计学意义(P<0.05)。治疗后观察组总有效率为94.81%,对照组为88.72%,两组比较,差异无统计学意义(P>0.05)。治疗期间,观察组血压升高患者数量低于对照组,差异有统计学意义(P<0.05),两组患者骨质疏松、消化道症状不良反应发生率比较,差异无统计学意义(P>0.05)。结论强肌健力胶囊治疗MG疗效显著,可明显改善机体免疫功能,同时不会增加不良反应发生率。
文摘目的:观察参地颗粒对慢性肾炎脾肾亏虚型患者Th17细胞免疫功能的干预作用。方法:90例慢性肾炎患者按随机数字表法分为试验组和对照组各45例,2组均给予西医基础治疗,对照组加用缬沙坦胶囊(代文)80 mg/d,试验组在对照组基础上加用参地颗粒,2组疗程均为12周。观察24 h尿蛋白定量(24 h Upro)、尿红细胞计数(URBC),并采用流式细胞技术检测外周血CD4^+、CD8^+和Th17细胞含量,酶联免疫吸附法检测血清白介素-17(IL-17)水平。结果:试验组疾病疗效总有效率显著高于对照组。试验组治疗后24 h Upro、URBC与治疗前比较均显著降低,且24 h Upro亦显著低于同期治疗后的对照组。试验组治疗后的Th17含量及IL-17水平与治疗前比较均有显著降低,CD4^+、CD4^+/CD8^+比值显著提升,且其CD4^+/CD8^+比值、Th17含量及IL-17水平变化与对照组比较亦差异有统计学意义。结论:参地颗粒可以通过调节Th17细胞功能,从而改善慢性肾炎脾肾亏虚型患者的免疫功能,发挥临床疗效。
基金Supported by the Provincial Innovation Team for Cataract and Ocular Fundus Disease in the Second People’s Hospital of Yunnan Province(No.2017HC010)the Key Laboratory of Yunnan Province for the Prevention and Treatment of Ophthalmology(No.2017DG008)Expert Workstation of Yao Ke(No.2017IC064)。
文摘AIM:To investigate the feasibility and mechanism of immune tolerance in allergic conjunctivitis.METHODS:The allergic conjunctivitis immune tolerance mice model was established by ragweed pollen(RW)and the related cytokines were detected.The mice were divided into 9 groups and the maslinic acid(MA)or PBS were given for different group after modeling.The expression levels of chemokine ligand 5(CCL5)and P-65 in the conjunctival tissue were analyzed by immunohistochemistry,quantitative reverse transcription polymerase chain reaction(q RT-PCR)and Western blot.The percentage of interleukin-17(IL-17)and CD4+CD25+in the splenocyte supernatant was analyzed by flow cytometry.Fur thermore,the serum and splenocyte supernatant concentration of total-IgE,interleukin-10(IL-10),and IL-17 was analyzed by enzyme linked immune response(ELISA).RESULTS:After the model was established,symptoms of conjunctivitis were alleviated,the level of P-65,CCL5,IL-17,and total-IgE was raised,while the expression of IL-10,CD4+CD25+was decreased.This result fully demonstrated that a typical IL-17/regulatory-T-cells(Treg cells)imbalance and NF-κB activation.When the NF-κB signal pathway was suppressed,it showed that there was a further relief of conjunctivitis in mice.At the same time,the expression of total-IgE,IL-17,and CCL5 was decreased and the expression of anti-inflammatory factor(IL-10,CD4+CD25+)was increased.CONCLUSION:In the state of immune tolerance,symptoms of conjunctivitis in mice are alleviated,the Th-17 cells of allergic conjunctivitis mice are inhibited,and Treg cells activity is enhanced.
