目的观察苏沈九宝汤治疗支气管哮喘急性发作期的临床疗效和对Th1/Th2因子的影响。方法选取2022年2月至2023年5月于浙江中医药大学附属四省边际中医院诊治的98例支气管哮喘急性发作期患者,根据随机数字表法将其分为治疗组和对照组,每组...目的观察苏沈九宝汤治疗支气管哮喘急性发作期的临床疗效和对Th1/Th2因子的影响。方法选取2022年2月至2023年5月于浙江中医药大学附属四省边际中医院诊治的98例支气管哮喘急性发作期患者,根据随机数字表法将其分为治疗组和对照组,每组各49例。对照组患者给予吸氧、雾化等常规治疗,治疗组患者在对照组基础上加用苏沈九宝汤。比较两组患者的临床疗效、中医证候积分、肺功能指标[第一秒用力呼气量(forced expiratory volume in first second,FEV1)、呼气流量峰值(peak expiratory flow,PEF)]和实验室指标[白细胞介素(interleukin,IL)-4、IL-13、γ干扰素(interferon-γ,IFN-γ)、免疫球蛋白E(immunoglobulin E,IgE)]。结果治疗组患者的总有效率显著高于对照组(χ^(2)=9.287,P=0.010);治疗后,两组患者的中医证候积分、血清IL-4、IL-13、IgE水平均显著低于本组治疗前,FEV_(1)、PEF、IFN-γ水平均显著高于本组治疗前(P<0.05),治疗组患者的中医证候积分、血清IL-4、IL-13、IgE水平均显著低于对照组,FEV1、PEF、IFN-γ水平均显著高于对照组(P<0.05)。结论苏沈九宝汤治疗支气管哮喘急性发作,可显著提高临床疗效,降低患者的炎症因子水平,改善肺功能。展开更多
Objective: To explore the effect of PPAR γ agonist(rosiglitazone) on the secretion of Th2 cytokines and the proportion of immune cell subsets in asthma mice,Methods: Ovalbumin(OVA)-sensitized mice were used to build ...Objective: To explore the effect of PPAR γ agonist(rosiglitazone) on the secretion of Th2 cytokines and the proportion of immune cell subsets in asthma mice,Methods: Ovalbumin(OVA)-sensitized mice were used to build asthma models,Those mice were divided into the normal control group,model group and rosiglitazone group,Differences of the changes in lung histopathology of mice in the three group were observed through hematoxylin and eosin(HE) strain,and the numbers of the total cells,eosinophils and neutrophils in BALF of mice in the three groups were compared,ELISA and real-time PCR were employed to detect the protein levels of interleukin(IL)-5,IL-13,IL-4 and IL-10 and m RNA level,respectively,Flow cytometry number was implied to analyze the proportion of immune cell subsets in peripheral blood of mice,Results: Compared with the mice in the control group,and mice of the model group,the infiltration of inflammatory cells in BALF increased,bronchial smooth muscle became thickened,a large amount of collagen deposited,the secretion of Th2 cytokine increased significantly,the ratio of regulatory T cells(Treg) decreased,the ratio of T17 cells rose distinctly; while in mice of the rosiglitazone group,the changes of their lung histopathology were improved obviously,the number of infiltration of inflammatory cells declined,the thickened smooth muscle relieved,the deposition of collagen decreased,the secretion of Th2 cytokine was inhibited,the ratio of Treg went up,and the increased of the ratio of T17 cells was inhibited but still not return to normal level,Conclusions: Rosiglitazone can regulate the proportion of Treg and Th17 cells and inhibit the secretion of Th2 cytokines,which inhibit the airway inflammatory response for asthma mice effectively.展开更多
文摘目的观察苏沈九宝汤治疗支气管哮喘急性发作期的临床疗效和对Th1/Th2因子的影响。方法选取2022年2月至2023年5月于浙江中医药大学附属四省边际中医院诊治的98例支气管哮喘急性发作期患者,根据随机数字表法将其分为治疗组和对照组,每组各49例。对照组患者给予吸氧、雾化等常规治疗,治疗组患者在对照组基础上加用苏沈九宝汤。比较两组患者的临床疗效、中医证候积分、肺功能指标[第一秒用力呼气量(forced expiratory volume in first second,FEV1)、呼气流量峰值(peak expiratory flow,PEF)]和实验室指标[白细胞介素(interleukin,IL)-4、IL-13、γ干扰素(interferon-γ,IFN-γ)、免疫球蛋白E(immunoglobulin E,IgE)]。结果治疗组患者的总有效率显著高于对照组(χ^(2)=9.287,P=0.010);治疗后,两组患者的中医证候积分、血清IL-4、IL-13、IgE水平均显著低于本组治疗前,FEV_(1)、PEF、IFN-γ水平均显著高于本组治疗前(P<0.05),治疗组患者的中医证候积分、血清IL-4、IL-13、IgE水平均显著低于对照组,FEV1、PEF、IFN-γ水平均显著高于对照组(P<0.05)。结论苏沈九宝汤治疗支气管哮喘急性发作,可显著提高临床疗效,降低患者的炎症因子水平,改善肺功能。
基金Supported by medical research project of Health and Family Planning Commission of Chongqing(Grant No.20142019)
文摘Objective: To explore the effect of PPAR γ agonist(rosiglitazone) on the secretion of Th2 cytokines and the proportion of immune cell subsets in asthma mice,Methods: Ovalbumin(OVA)-sensitized mice were used to build asthma models,Those mice were divided into the normal control group,model group and rosiglitazone group,Differences of the changes in lung histopathology of mice in the three group were observed through hematoxylin and eosin(HE) strain,and the numbers of the total cells,eosinophils and neutrophils in BALF of mice in the three groups were compared,ELISA and real-time PCR were employed to detect the protein levels of interleukin(IL)-5,IL-13,IL-4 and IL-10 and m RNA level,respectively,Flow cytometry number was implied to analyze the proportion of immune cell subsets in peripheral blood of mice,Results: Compared with the mice in the control group,and mice of the model group,the infiltration of inflammatory cells in BALF increased,bronchial smooth muscle became thickened,a large amount of collagen deposited,the secretion of Th2 cytokine increased significantly,the ratio of regulatory T cells(Treg) decreased,the ratio of T17 cells rose distinctly; while in mice of the rosiglitazone group,the changes of their lung histopathology were improved obviously,the number of infiltration of inflammatory cells declined,the thickened smooth muscle relieved,the deposition of collagen decreased,the secretion of Th2 cytokine was inhibited,the ratio of Treg went up,and the increased of the ratio of T17 cells was inhibited but still not return to normal level,Conclusions: Rosiglitazone can regulate the proportion of Treg and Th17 cells and inhibit the secretion of Th2 cytokines,which inhibit the airway inflammatory response for asthma mice effectively.
