Thanks to our reviewers in 2022

The editorial team of the International Journal of Nursing Sciences would like to thank our peer reviewers for all their support in 2022.It is based on the dedication and expertise of the editorial board and peer reviewers to promote the academic quality and impact of the journal consistently.IJNSS will receive the first impact factor in 2023.If you would like to serve as a peer reviewer for the journal.

诱导活化的外周血单个核细胞THANK表达的初步研究

目的分析不同刺激下外周血单个核细胞(PBMC)中THANK基因的表达，并克隆全长的人THANK基因。方法采用将常规方法分离的外周血单个核细胞(PBMC)培养于含100ml/L FCS的RPMI 1640中，并以不同浓度的LPS、TNF-α、IL-2、IFN-γ、PHA及PMA刺激不同时间。以RT-PCR法，分析PMBC中THANK基因的转录表达，并克隆相应的全长人THANK基因。结果RT-PCR分析表明，当用IFN-γ刺激PMBC 72h后，可诱导THANK基因明显表达;而IL-2、LPS、TNF-α、PHA和PMA则不能诱导其表达。采用PCR产物克隆的方法，克隆了人THANK基因，并经DNA序列测定证实。结论克隆得到了人THANK基因，为进一步研究THANK的功能打下了基础。

THANK基因的克隆和序列分析

目的克隆THANK基因全长及胞 外区片段并测序。方法采用RT-PCR技术,从人白血 病细胞系HL-60细胞总RNA中扩增人THANK cDNA,并定向克隆于pMD-18 T载体,转 染大肠杆菌、抽提质粒后,用ABI PRISM^TM 377XL DNA自动测序仪测序。结果RT-PCR扩增出一个858bp的DNA片段,限制性内 切酶图谱分析和测序结果显示:该858bp片段为编码人THANK的cDNA,与公布的人THANK 基因序列一致。结论本实验成功地克隆了人THANK基 因,为进一步表达人THANK基因并研究其功能,开发与临床应用奠定了基础。

人THANK基因的克隆和表达

目的 :克隆人 THANK基因全长及胞外区片段 ,将其胞外区在大肠杆菌中表达。 方法 :采用 RT- PCR技术 ,从人白血病细胞系 HL - 6 0细胞总 RNA中扩增人 THANK c DNA ,并定向克隆于 p MD18- T载体 ,经测序证实 ,再亚克隆 THANK的胞外区片段至 p ET表达载体 ,在大肠杆菌中进行表达。 结果 :RT- PCR扩增出一个 85 8bp的 DNA片段 ,该片段与公布的人THANK基因序列一致。诱导后 THANK胞外区在大肠杆菌中可表达出相对分子质量为 2 .6万的蛋白质。结论 :成功地克隆了人 THANK基因 ,并在大肠杆菌中获得表达 ,为其功能的研究打下基础。

重组THANK诱导表达和变性、复性及纯化

目的 :获得较纯的具有生物学活性的 THANK蛋白。方法 :THANK基因在大肠杆菌中高效表达 ,菌体超声破碎后 ,以洗涤剂反复洗涤包涵体。包涵体经 8mol/L尿素变性溶解后 ,再用 Sephacryl S- 2 0 0凝胶过滤层析初步纯化 ,对重组蛋白浓度、氧化还原剂等复性参数进行优化和选择 ,将蛋白稀释复性。复性后组分经 Q Sapharose Fast Flow离子交换层析再次纯化 ,最后以 Sep hadex G- 2 5脱盐。 结果 :得到了纯度 >97%、具有一定生物学活性的 THANK蛋白。 结论 :THANK蛋白变性、复性及纯化方法的建立 ,为

腺病毒介导的THANK基因在SMMC-7721细胞中的表达

目的:构建THANK腺病毒载体,观察腺病毒感染后THANK在SMMC-7721细胞中的表达情况。方法:把THANK基因克隆入腺病毒穿梭载体pAdTrackCMV中,与腺病毒骨架质粒pAdEasy1共转染大肠杆菌BJ5183,获得腺病毒重组质粒,再将获取的腺病毒重组质粒转染入293细胞进行包装,获取THANK重组腺病毒,以不同感染复数(MOI)腺病毒感染SMMC-7721细胞,观察腺病毒对SMMC7721细胞的感染情况。结果:成功地构建了THANK腺病毒,该腺病毒可高效介导THANK在SMMC-7721细胞中表达,基因转染后第5天,THANK表达高于35ng/ml,且随时间延长增加。结论:腺病毒可诱导THANK基因在SMMC-7721细胞中高表达。

重组人可溶性THANK在大肠杆菌中的高效表达

目的 :克隆人 THANK的全长基因及编码可溶性胞外区的基因 ,并在大肠杆菌中表达可溶性 THANK。 方法 :采用RT- PCR从 PMA诱导的 HL 6 0细胞中克隆人 THANK全长编码基因 ,继以 PCR扩增出可溶性 THANK编码区基因 (134～2 85位氨基酸 ) ,PCR产物克隆于 p MD- 18T,经 DNA测序证实后亚克隆到 p ET- 11a中。重组质粒转化 BL 2 1,以 1m mol/ LIPTG进行诱导表达产物 ,SDS- PAGE分析表达产物 ,对重组蛋白经纯化复性后测定生物学活性。结果 :RT- PCR扩增出编码人 THANK全长编码基因及 PCR扩增出 THANK1 34 - 2 85基因的 c DNA ,经序列分析证实与文献报道完全一致。含有THANK1 34 - 2 85编码基因的表达载体 ,转化大肠杆菌后表达出相对分子质量约 180 0 0的重组蛋白。该重组蛋白经纯化后能够诱导 U937细胞凋亡。结论 :成功克隆了人 THANK基因 ,并在大肠杆菌中表达重组可溶性 THANK,该重组蛋白在实验条件下具有诱导 U937细胞凋亡的作用。

人THANK基因转染人肝癌细胞及生物学特性

[目的]将人THANK基因转染入人肝癌细胞SMMU-7721中稳定表达,检测转染后细胞的生物学特性变化。[方法]将由pMD18-T-THANK质粒上获得的THANK全长cDNA克隆到真核表达载体pcDNA3.1中;用脂质体法将pcDNA3-THANK导入人肝癌细胞株SMMU-7721中,G418筛选克隆,再以RT-PCR及Westernblot检测THANK的表达,流式细胞仪及细胞毒杀伤实验分析转染后细胞的生物学特性变化。[结果]THANK在7721细胞中的表达可抑制7721细胞的生长,使肿瘤细胞的生长停滞于S期,且可在体外诱导强烈的细胞毒性T淋巴细胞(CTL)反应,人外周血单个核细胞(PBMC)对THANK-7721细胞的杀伤活性明显增强。THANK的转染与IFN-γ协同可增强7721细胞的凋亡比例。[结论]THANK基因的转染改变了7721细胞的生物特性,在体外诱导PBMC的CTL反应,有可能用于肝癌的免疫治疗。

对称赞语回应之“thank you”的研究(英文)

致谢作为一种和谐性的言语行为，本质上是礼貌的，与道歉，命令，祝贺和许诺一样，它与人们的日常生活密切相关。致谢同时又是我们日常生活中发生频率很高的一种言语行为。正确地表达致谢有着重要的社会价值。本研究从理论上增进了我们有关致谢言语行为的知识，从实践上可以帮助我们建立一个良好的人际关系和和谐的社会氛围。

THANK—一种新的免疫调节因子

THANK是 1999年发现的肿瘤坏死因子超家族成员。最早发现其可诱导肿瘤细胞凋亡 ;之后则发现THANK和TNF家族的另一成员APRIL与它们的两个受体TACI和BCMA形成了双配体 双受体系统 ,共同调节B细胞的活化、增生与成熟 ;而TACI表达于活化的T细胞及可诱导NFAT的表达 ,表明THANK不仅与B细胞的功能有关 ,且参与了T细胞介导的免疫调节。THANK对B细胞的作用大大地加深了人们对B细胞生长、发育调控的理解。本文对THANK基因的研究进展进行了综述。

In Vitro and in Vivo Study of the Antitumor Effects of a THANK Modified Hepatoma Cell Line

Objective THANK, known as a member of TNF superfamily, is a potent costimulator of both B and T lymphocytes and can promote astrong immune response. To investigate its role in liver immunotherapy, the anti-tumor effects of the THANK-transduced hepatoma cellline SMMU-7721 in vitro and in vivo were studied.Methods THANK full-length cDNA was transfected into SMMU-7721 cell line. The transfectant with stable expression of THANK wasobtained by clone selection and THANK s effects on hepatoma cells were analyzed, further the tumorigenicity of THANK-transduced7721 cells was examined in nude mice.Results THANK's expression in 7721 cells inhibited the growth of hepatoma cells and induced a strong CTL response in vitro. The cellcycle analysis showed that THANK transfected 7721 cells were arrested in the S phase. The expression of THANK in SMMU-7721 cellline not only inhibited the tumorigenicity of 7721 cells, but also induced a systemic immune response against re-challenge of parental7721 tumors.Conclusion THANK transduction in SMMU-7721 cells can induce an effective immune response in nude mice and may be useful for theimmunotherapy of hepatomas.

Thank You, Challenges!

<正>A person may meet many challenges in his or her life.Some people think challenges are terrible.But I think they’re great.And I want to say,'Thank you,challenges!'For some people,it’s hard to face the challenges because they are afraid of failing in the end.However,it’s necessary for a person to face the challenges.Challenges are good to~①you.

Journal of Natural Gas Chemistrv thanks the authors of our most cited articles

According to the Thomson Reuter report on 28 June 2012, the 2011 Impact Factor of Journal of Natural Gas Chemistry is 1.348, which ranks first among chemistry journals in China. We would like to express our appreciation to all the authors who have contributed their good work to the Journal. The following is the list of the articles published in 2009 and 2010 which received the most citations and thus contributed the most to the new impact factor.

Thanks

The editorial staff of the Hepatobiliary ＆ Pancreatic Diseases International are grateful to the listed reviewers for their contributions to the improvement of the manuscripts received and the publication of the journal in the year of 2016 （November 1, 2015 to October 31, 2016）.

Thanks

The editorial staff of the Hepatobiliary & Pancreatic Diseases International are grateful to the listedreviewers for their contributions to the improvement of the manuscripts received and the publication of

Thank you to our peer reviewers

The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the following peer reviewers for their selfless devotion of time and energy to the journal in the year 2012：

Thanks

The editorial staff of the Hepatobiliary & Pancreatic Diseases International are grateful to the listed reviewers for their contributions to the improvement of the manuscripts received and the publication of the journal in the year of 2007.

Thanks

The editorial staff of the Hepatobiliary&Pancreatic Diseases International are grateful to the listed reviewers for their contributions to the improvement of the manuscripts received and the publication of