Effects of Inoculation with Phosphate Solubilizing Bacteria on the Physiology,Biochemistry,and Expression of Genes Related to the Protective Enzyme System of Fritillaria taipaiensis P.Y.Li

Fritillaria taipaiensis P.Y.Li is a widely used medicinal herb in treating pulmonary diseases.In recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly increased.This article is the first to apply phosphate solubilizing bacteria isolated from the rhizosphere soil of F.taipaiensis P.Y.Li to the cultivation process of F.taipaiensis P.Y.Li.The aim is to identify suitable reference strains for the artificial cultivation and industrial development of F.taipaiensis P.Y.Li by examining the effects of various phosphate solubilizing bacteria and their combinations on photosynthesis,physiological and biochemical properties,and gene expression related to the protective enzyme system in F.taipaiensis P.Y.Li.The experiment,conducted in pots at room temperature,included a control group(CK)and groups inoculated with inorganic phosphorussolubilizing bacteria:W1(Bacillus cereus),W2(Serratia plymuthica),W12(Bacillus cereus and Serratia plymuthica),and groups inoculated with organophosphorus-solubilizing bacteria:Y1(Bacillus cereus),Y2(Bacillus cereus),Y12(Bacillus cereus and Bacillus cereus),totaling seven groups.Compared to CK,most growth indices in the bacterial addition groups showed significant differences,with W12 achieving the highest values in all indices except the leaf area index.The content of photosynthetic pigments,photosynthetic parameters,and osmoregulatory substances increased variably in each bacterial treatment group.W12 exhibited the highest content of chlorophyll a and soluble protein,while W1 had the highest free proline content.The activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT)in all inoculated groups were higher than in CK,with significant changes in SOD and CAT activities.The malondialdehyde(MDA)content in all inoculated groups was lower than in CK,with Y12 being the lowest,at approximately 30%of CK.Gene expression corresponding to these three enzymes also increased variably,with POD expression in Y2 being the highest at 2.73 times that of CK.SOD and CAT expression in Y12 were the highest,at 1.84 and 4.39 times that of CK,respectively.These results indicate that inoculating phosphate solubilizing bacteria can enhance the growth of F.taipaiensis P.Y.Li,with the mixed inoculation groups W12 and Y12 demonstrating superior effects.This lays a theoretical foundation for selecting bacterial fertilizers in the cultivation process of F.taipaiensis P.Y.Li.

Effects of Heavy Metals and Saline-alkali on Growth,Physiology and Biochemistry of Orychophragmus violaceus

[Objective] The aim was to study on effects of heavy metals and saline-alkali on growth, physiology and biochemistry of Orychophragmus violaceus. [Method] Taken Orychophragmus violaceus as materials, growth, physiology and biochemistry were explored under stress of saline-alkali and heavy metals (light, moderate and severe saline-alkali, Pb, Pb + Cd, light saline-alkali + Pb, moderate saline-alkali + Pb, severe saline-alkali + Pb, light saline-alkali + Pb + Cd, moderate saline-alkali + Pb + Cd and severe saline-alkali + Pb + Cd) with control group set. [Result] Light stress of saline-alkali had little effect on membrane permeability, as follows: MDA contents in leaves and root systems declined by 25.6% and 9.0% compared with control group; Pb (500 mg/L) stress promoted synthetization of photosynthetic pigments, as follows: chlorophyll a and b and carotenoid increased by 0.86%, 0.69% and 6.25% than those of control group; combined stresses of Pb and Cd destroyed synthetization of photosynthetic pigments, among which carotenoid was more sensitive; under combined stresses of saline-alkali, Pb and Cd, POD and SOD activities, soluble saccharides and Pro content all increased and activities of POD and SOD in root system were both higher than those in leaves. [Conclusion] Orychophragmus violaceus is with resistance against light combined stresses of saline-alkali and Pb (500 mg/L).

Effects of Potassium-Solubilizing Bacteria on Growth, Antioxidant Activity and Expression of Related Genes in Fritillaria taipaiensis P. Y. Li

This study aimed to examine the effects of inoculating Fritillaria taipaiensis P.Y.Li leaves with different strains ofpotassium-solubilizing bacteria (KSB), or combinations thereof, focusing on aspects of photosynthesis and physiologicaland biochemical characteristics. At present, some studies have only studied the rhizosphere microbialcommunity characteristics of F. taipaiensis and have not discussed the effects of different microbial species on thegrowth promotion of F. taipaiensis. This paper will start from the perspective of potassium-solubilizing bacteria toconduct an in-depth study. Seed cultivation commenced at the base with three different KSBs in early October2022. The growth of F. taipaiensis leaves was observed after different treatments. Both single-plant and compoundinoculations were executed. A total of eight treatment groups were established, with aseptic fertilizer and sterilizedsoil functioning as the control group. The results reveal that intercellular CO_(2) concentration (Ci), stomatal conductance(Gs), and transpiration rate (Tr) were at their apex in the S7 group. Most treatment groups exhibited anincrease in leaf area, photosynthetic pigment content, soluble sugar, soluble protein, Superoxide Dismutase(SOD), Peroxidase (POD), Catalase (CAT) activities, and proline content. The expression levels of POD, SOD,and CAT genes were evaluated, following inoculation with different KSB. The highest was the S7 group. Theinoculation with various KSB, or combinations thereof, appears to bolster the growth and development of F. taipaiensis.The composite inoculation group S7, comprising Bacillus cereus, Burkholderia cepacia, and Bacillus subtilis,manifested the most favorable impact on the diverse indices of F. taipaiensis, thereby furnishing valuableinsights for the selection of bacterial fertilizer in the artificial cultivation of F. taipaiensis.

Effect of different anesthetic mixtures——ketamine-xylazine, ketamine-acepromazine and tiletamine-zolazepam——on the physiological and blood biochemistry parameters of male rhesus monkeys(Macaca mulatta) at different ages

Background: Anesthetic agents are commonly utilized in the handling of non‐human primates for prevent the stress caused in physical exploration or physical restrain. For this reason, the objective of this work was to describe the effect of age and dissociative anesthetics (ketamine and tiletamine), and their combinations with acepromazine, xylazine and zolazepam, on the physiological and blood biochemical parameters in Macaca mulatta. Methods: Eighty male Macaca mulatta were divided into four experimental groups depending on the anesthetic mixture applied. Each group of 20 males was divided into five sub‐groups according to age. Physiological parameters were recorded every five minutes during a 30‐minute period. A blood sample was drawn to analyze blood biochemistry. Results: Statistical analyses revealed significant differences in the physiological parameters between the ketamine‐acepromazine and ketamine‐xylazine groups compared to the control group. The analysis of blood biochemistry found significant differences by age and by anesthetic mixture among all groups. Conclusion: These findings contribute to standardizing this animal model in biological research.

The Effects of Building Form on Microclimate and Outdoor Thermal Comfort in a Tropical City

The urban microclimate has direct implications with regards to thermal comfort indoors as well as outdoors. In the tropics, the outdoor thermal comfort conditions during daytime are often far above acceptable comfort standards due to intense solar radiation and high solar elevations. This study aims to know effects of simple and fundamental building forms on microclimate and outdoor thermal comfort in a high dense tropical city, focusing on Dhaka, Bangladesh as a study city. Investigations are carried out on existing area and model areas with modified building forms （in respect of height and shape） on the microclimate as well as on outdoor thermal comfort during daytime in summer. This study has demonstrated that the model using less ground coverage and higher buildings can offer a better thermal climate than the models using maximum ground coverage in a high-density tropical city.

Effect and Mechanism of Cold Tolerant Seed-Coating Agents on the Cold Tolerance of Early Indica Rice Seedlings

To better understand the effect and mechanism of cold tolerant seed-coating agents on the cold tolerance of rice seedlings, the physiological and biochemical effects of four cold tolerant seed-coating agents （HET, YKJ, YKZYJ, and the ABA seed coating agents） on two early indica rice varieties were studied under chilling stress. The results showed that the rice seedlings treated with cold tolerant seed-coating agents under chilling stress maintained dramatically higher root vigor, POD, CAT and SOD activities, and chlorophyll content, had lower MDA content and electrolyte leakage, and accumulated more soluble sugar and free proline, when compared with the control without the treatment, and finally showed lower plant injury rate. It was indicated that the cold tolerant seed coating agent improved the ability of rice seedlings in resisting to chilling stress. YKZYJ was ranked the first in terms of the efficiency in cold tolerance among the four cold tolerant seed-coating agents tested.

Research progress in physiological and molecular biology mechanism of drought resistance in rice

Rice is one of the most important crops, providing staple food for about half population of the world. Drought stress affects plant growth and development seriously. This article reviewed the research progress of the physiological and molecular biology mechanism including osmotic adjustment, scavenging oxidative radicals, endogenous hormones, drought-resistance genes and epigenetic modification, it may be afford interrelated reference for increasing rice drought resistance and breeding drought resistance rice varieties.

Effects of Hypobaric Storage on Physiological and Biochemical Changes in Postharvest Dong Jujube Fruit During Cold Storage

Effects of hypobaric storage on physiological and biochemical changes in Dong jujube fruit were investigated. Hypobaric storage significantly delayed the decrease in firmness and maintained content of ascorbic acid, reduced accumulation of ethanol and acetaldehyde in pulp and respiration, inhibited activities of ascorbic acid oxidase and alcohol dehydrogenase and slowed down the rate of ethylene production, but had little effect on flesh browning of the fruit.

Effects of Stratification and Hormone Treatments on Germination and Physio-Biochemical Properties of <i>Taxus chinensis var. mairei</i>Seed

In order to learn dormancy mechanism of Taxtts chinensis var. mairei seed, effects of stratification and hormone treatments on physiological and biochemical indexes of the seeds after stratifying for eight months were researched. The content of amylase activity and soluble sugar gradually increased at first stratification, and then to the maximum for the end of after-ripening period. With the dormancy of seed relieving, some soluble sugar of endosperm was used or consumed by respiration and that led to their content decreased. Seed soluble protein was gradually accumulated at the beginning of stratification and then the initial raw material of protein synthesis of embryo during development had been used or in part converted into carbohydrates for energy consumption, so that the soluble protein content decreased. The fat and starch content slowly decreased during stratification, which showed that the storage of material had continually carrying out catabolism and conversion, which might provide energy sources for seed germination in the stratification process. The peroxidase activity of Taxus seeds increased gradually at the beginning of stratification, and then reached maximum point during stratification for 4 months in A1 and A2. The G-6-PDH and 6-PGDH joint activity was rapidly increased to reach the maximum during stratification for 4 months in A1, which showed the pentose phosphate pathway was activated at this time. The peroxidase activity rapidly increased might also be related to respiratory metabolic pathway during stratification. In plants, acid phosphatase hydrolyze PAM metabolites, namely phosphate material was related to energy metabolism, especially to high-energy phosphate, and phosphate enzyme activity changes affected the energy metabolism. The acid phosphatase activity of Taxus seeds in different treatments has no significant changes during stratification process, but there were two maximums. Energy metabolism might get benefit from the increase of acid phosphatase activity. There were significant differences in amylase activity, peroxidase activity, soluble sugar and soluble protein content with different treatments. Effect of promoting seed germination soaked by GA before stratification was obvious, but the concentration of GA should not be too high. The high concentration of GA caused seed to rot and reduce the seed germination rate. In this study, the highest germination rate of seed was 71.8% and soaked in 200 mg·L-1 gibberellin.

玉米苯丙氨酸解氨酶(PAL)家族基因分析及ZmPAL5的功能研究

【目的】挖掘玉米抗旱关键基因、揭示其抗旱分子机制,为培育抗旱玉米新品种提供基因资源和理论指导。【方法】采用转录组数据结合加权基因共表达网络(weighted gene co-expression network,WGCNA)与抗旱性生理生化指标的筛选方法,鉴定与抗旱和复水相关的ZmPAL基因。利用生物信息学方法对编码PAL的基因进行全基因组分析。运用实时荧光定量PCR(quantitative real-time polymerase chain reaction,qRT-PCR)检测ZmPAL基因在干旱处理条件下的表达情况,以及ZmPAL5在不同自交系间的表达特性和在不同组织中的表达模式。最后,利用遗传转化分析ZmPAL5在玉米中的抗旱功能,并借助CRISPR/Cas9技术对PAL5同源基因进行缺失型拟南芥突变体的抗旱性分析。【结果】鉴定了19个玉米ZmPAL基因,其中6个基因聚集在第5染色体上,其编码蛋白多为亲水性酸性蛋白,且PAL家族基因的进化相对保守。ZmPAL基因启动子区域含有大量与激素和非生物应激响应相关的顺式作用元件。确定了6个核心基因,其中4个基因在干旱处理后显著上调表达。尤其是ZmPAL5在干旱胁迫后表达量增加了8.57倍。在干旱胁迫和复水处理条件下,发现抗旱自交系郑8713中ZmPAL5的表达水平显著高于旱敏感自交系B73。同时,ZmPAL5是一个组成型表达基因,在幼茎中表现出高水平的表达。过表达ZmPAL5玉米在干旱胁迫下生长良好,其相对含水量、木质素、叶绿素、可溶性蛋白、脯氨酸含量,以及超氧化物歧化酶、过氧化物酶、过氧化氢酶和抗坏血酸过氧化物酶的活性分别是野生型的1.52、1.49、1.47、1.43、1.44、1.41、1.53、1.41和1.35倍,但丙二醛含量是野生型的0.65倍。PAL5缺失型拟南芥突变体对干旱敏感。在干旱胁迫下,其生理生化指标与过表达ZmPAL5玉米的指标变化趋势相反。【结论】筛选出6个响应干旱胁迫的核心基因(ZmPAL3、ZmPAL5、ZmPAL6、ZmPAL8、ZmPAL11和ZmPAL13),其中,ZmPAL5的表达量与抗旱性呈正相关。ZmPAL5通过影响渗透调节物质含量和抗氧化酶活性正向调节植物的抗旱性和恢复能力。

不同叶果比对设施‘红美人’杂柑秋梢叶片生理生化特征的影响

为探究不同叶果比(leaffruitratio,LFR)对设施柑橘秋梢叶片生理生化特征的影响,以设施栽培‘红美人’杂柑为试材,设置高(LFR=100)、中(LFR=80)、低(LFR=60)3个叶果比,测定秋梢叶片中矿质元素、碳氮代谢物、碳氮代谢的关键酶、抗氧化酶活性等生理指标的变化。结果显示,LFR80和LFR100秋梢叶片的磷、钾、镁、淀粉含量较高;丙二醛、脯氨酸、抗坏血酸含量较低;β-淀粉酶、硝酸还原酶、谷氨酰胺合成酶和抗坏血酸过氧化物酶的活性较高;LFR80和LFR100秋梢叶片整体养分含量充足,受胁迫程度较低。结果表明,设施栽培‘红美人’杂柑的叶果比控制在80~100有利用于平衡营养生长和生殖生长,延缓叶片衰老,维持树势强健。

菠萝抗寒研究进展

低温是限制菠萝产量和品质的重要因素之一。我国菠萝种植适宜区冬季周期性地受到低温等自然灾害影响,且近年来频繁遭遇严峻气候环境挑战,造成减产或失收,严重影响菠萝产业可持续发展。目前,关于菠萝抗寒性研究主要集中于抗寒种质资源鉴定与评价、抗寒性评价方法、生理生化测定、抗寒基因挖掘和抗寒防治措施等方面,而在菠萝抗寒性分子机制方面的研究进展较缓慢,本文针对这些方面进行概述,以期为菠萝抗寒性状的遗传改良及生产上防寒防冻提供参考。

PEG6000模拟干旱胁迫对铁皮石斛幼苗生理和叶绿素荧光特性的影响

【目的】研究聚乙二醇6000(PEG 6000)模拟干旱对铁皮石斛Dendrobium candidum生理和叶绿素荧光特性的影响,为铁皮石斛的品种选育、产业化栽培和近野生栽培等提供参考。【方法】以铁皮石斛‘晶品1号’D.candidum‘Jingpin No.1’幼苗为实验材料,通过不同质量分数PEG 6000(5%、10%、20%、30%)模拟干旱胁迫处理铁皮石斛幼苗,观察铁皮石斛幼苗茎段和叶片细胞结构,并检测铁皮石斛叶片过氧化物酶(POD)、过氧化氢酶(CAT)活性及丙二醛(MDA)质量摩尔浓度、可溶性糖质量分数、可溶性蛋白质量分数、叶绿素质量分数及叶绿素荧光参数的动态变化。【结果】①高质量分数PEG 6000(20%-30%)处理后铁皮石斛茎段和叶片细胞内叶绿素质量分数减少。②PEG 6000模拟干旱胁迫处理对铁皮石斛幼苗可溶性糖和可溶性蛋白、MDA、POD和CAT有显著影响(P<0.05)。可溶性糖质量分数随着PEG 6000质量分数的增加和处理时间的延长均呈上升趋势,到第12天时达最高值;可溶性蛋白则呈下降趋势;MDA质量摩尔浓度、POD活性和CAT活性随着PEG 6000质量分数的增加呈先上升后下降的趋势,在PEG 6000为20%时达到峰值。③PEG 6000模拟干旱处理对铁皮石斛幼苗最大光化学效率(F_(v)/F_(m))、光合效率(α)、电子传递速度(ETR)、光化学猝灭系数(q_(P))和非化学猝灭系数(qNP)等叶绿素荧光参数有显著的影响(P<0.05)。随着PEG 6000胁迫处理时间的延长和PEG 6000质量分数的增加,α、ETR、F_(v)/F_(m)和q_(P)均呈显著下降趋势(P<0.05);而qNP则呈先上升后下降的趋势。【结论】20%PEG 6000处理12 d可用作铁皮石斛抗干旱品种的筛选,铁皮石斛幼苗可通过增加可溶性糖质量分数,减少可溶性蛋白质量分数,提高POD和CAT活性等防御酶活性抵抗和适应一定程度的干旱胁迫;铁皮石斛幼苗的最大光能转换效率降低,光合系统Ⅱ(PSⅡ)受到胁迫损伤,严重影响铁皮石斛幼苗的光合作用。同时,铁皮石斛幼苗通过启动qNP途径来消耗PSⅡ反应中心吸收的过剩光能,维持正常的光合作用。因此,可溶性糖和可溶性蛋白、POD和CAT以及叶绿体荧光参数均可作为铁皮石斛耐干旱的指标。

道路运输对羔羊体重、血液生化及临床指标的影响

试验旨在研究道路运输对羔羊体重、血液生化指标和临床指标的影响,为生产中有效缓解羔羊运输应激提供理论依据。选取平均体重(21.52±1.22)kg健康4月龄羔羊10只进行8 h的长途运输,分别在运输前后进行称重、静脉采血,并测定体温、呼吸频率、脉搏等临床指标。结果表明:①道路运输使羔羊体温、呼吸和脉搏频率极显著升高(P<0.01),体重降低了15.31%(P<0.05)。②道路运输极显著升高了羔羊血清中促肾上腺皮质激素(ACTH)和乳酸脱氢酶(LDH)水平(P<0.01),显著升高了皮质醇(COR)和肌酸激酶(CK)水平(P<0.05),血清中谷丙转氨酶(ALT)水平无显著变化(P>0.05)。③道路运输显著降低了羔羊血清中免疫球蛋白A(IgA)、免疫球蛋白G(IgG)水平(P<0.05),显著升高了白介素-6(IL-6)水平(P<0.05),血清中免疫球蛋白M(IgM)、干扰素-γ(IFN-γ)、肿瘤坏死因子α(TNF-α)、白介素-1β(IL-1β)水平与运输前差异不显著(P>0.05)。④道路运输显著降低了羔羊血清中葡萄糖(GLU)、总蛋白(TP)、白蛋白(ALB)水平(P<0.05)。⑤道路运输显著降低了羔羊血清中红细胞总数(RBC)、红细胞压积(HCT)、血红蛋白含量(MCH)(P<0.05),红细胞平均体积(MCV)极显著降低(P<0.01),白细胞总数(WBC)显著升高(P<0.05)。综上所述,羔羊经过8 h道路运输后发生了明显的应激反应,导致羔羊体重下降,机体代谢增强,炎症反应增强,免疫系统受到了一定程度损伤。

腐殖质对干旱胁迫下多花黄精叶片生理生化特性的影响

【目的】通过研究干旱胁迫下多花黄精生理生化指标的变化,综合评价腐殖质对其抵御干旱胁迫的影响,为多花黄精节水栽培提供理论依据。【方法】采用盆栽实验,分别以腐殖质土和黄土(对照基质)为培养基质,设置正常供水(土壤含水量80%~85%)、轻度干旱(土壤含水量60%~65%)、中度干旱(土壤含水量40%~45%)、重度干旱(土壤含水量20%~25%)4个控水梯度,测定多花黄精叶片相对电导率、丙二醛含量、可溶性糖含量、可溶性蛋白含量、超氧化物歧化酶活性和过氧化物酶活性等生理生化指标的变化。【结果】轻度干旱胁迫不会导致多花黄精叶片生理生化抗旱指标发生明显变化;中度干旱胁迫对多花黄精叶片生理生化产生较明显影响,腐殖质土基质中多花黄精叶片相对含水量、叶绿素含量、SOD活性、POD活性比对照基质分别少下降0.34%、4.99%、18.37%、2.90%,相对电导率、MDA含量分别少增加10.30%、9.12%,可溶性糖含量、可溶性蛋白含量分别多增加3.60%、1.79%;重度干旱胁迫对多花黄精叶片生理生化产生明显影响,腐殖质土基质下多花黄精叶片的相对含水量、叶绿素含量、SOD活性、POD活性比对照基质分别少降低2.16%、5.14%、19.92%、8.56%,相对电导率、MDA含量分别少增加14.16%、16.86%,可溶性糖含量、可溶性蛋白含量分别多增加4.06%、2.35%。隶属函数法综合评判得出腐殖质土基质栽培下多花黄精抗旱隶属函数值比对照基质提高了151%。【结论】腐殖质作为多花黄精促生长的栽培基质,在干旱胁迫下可提高多花黄精生理生化抗旱指标的稳定性,并通过增加渗透调节物质含量的方式在一定程度上抵御干旱胁迫。

水稻对干旱胁迫的生理生化响应及分子调控研究进展

水稻是全球最重要的粮食作物之一,其生长过程需要大量水分。随着全球气候变暖,干旱成为其产量的重要限制因素。因此,本文结合近些年的研究成果从形态(根系和地上部)、生理(气孔、蒸腾作用、光合作用和水分利用率)、生化(植物激素、脯氨酸等渗透调节剂和抗氧化剂)及分子水平(抗旱基因的表达水平)综述了水稻在干旱胁迫下的自我保护机制,可为全面了解水稻抗旱机制和选育抗旱品种提供参考。

高寒湿地植物对水淹胁迫的生理响应及适应性评价

为探究高寒湿地植物的耐水淹机理,测定杉叶藻(Hippuris vulgaris)等5种高寒湿地植物水淹40 d后的形态和生理生化指标,利用耐水淹系数,采用PCA分析、相关性分析和隶属函数法构建水淹胁迫适应性评价体系。结果表明:水淹胁迫下5种植物抗氧化酶活性上升1~4倍,以抵抗水淹胁迫,适应水淹环境;不同植物地上部分和地下部分丙二醛含量、抗氧化酶活性和渗透调节物质对水淹胁迫的响应有所不同;通过PCA分析和相关性分析,株高、光合色素含量、SS含量、SP含量、地上部分POD活性和地上部分SOD活性可作为高寒湿地植物水淹胁迫适应性筛选的主要评价指标;对综合指标(PC1~4)进行加权和隶属函数分析,5种高寒湿地植物水淹胁迫适应性由强到弱顺序为杉叶藻>三裂碱毛茛>光稃早熟禾>双柱头蔺藨草>中国马先蒿。

高温高湿环境下运动员身体机能变化的研究进展

高温天气是运动训练、比赛中的一种特殊环境,可使运动员的体能消耗加剧、运动表现变差。高温通常伴随着较高湿度,湿度升高会进一步阻碍热量散失。近年来对高温环境下身体机能变化的研究主要集中在热应激和热适应中能量代谢及体温调节上。该文就高温环境对运动表现及身体机能生理生化影响方面进行综述,旨在为运动员在高温环境下训练和比赛对运动能力发挥提供理论指导与措施。

盐度驯化对大鳞鲃幼鱼生理生化和抗氧化免疫的影响

为了探索大鳞鲃经盐度驯化后的血液生理生化、免疫应激的适应性变化,选择体长为(15.09±3.54) cm,体重为(13.66±1.26) g的幼鱼,设置了3个组别,空白组始终处于淡水中养殖,驯化组经4 g/L的盐度适应性驯养168 h后再放入8 g/L的盐度水体中,未驯化组直接放入8 g/L的盐度水体中,分别测定并比较放入8 g/L盐度水体中第0、6、12、24、48、96和168小时幼鱼血液生理生化和免疫应激相关指标变化。结果显示,(1)驯化组和未驯化组幼鱼血液中的血常规指标[白细胞(WBC)、淋巴细胞(Lymph)、单核细胞(Mon)、中性粒细胞(Gran)、红细胞(RBC)、血红蛋白(HGB)含量]、血浆生理生化指标[尿素(UREA)浓度、血清葡萄糖(GLU-G)和白蛋白(ALB)含量、血浆渗透压]、鳃组织中的天门冬氨酸氨基转移酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)和肝脏组织中抗氧化指标[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽酶(GSH-Px)、丙二醛(MDA)含量]在盐度为8 g/L的水体中,均随着胁迫时间的延长呈现出先升高后降低的现象。(2)胁迫初期,驯化组和未驯化组幼鱼的各生化指标在相同组织内到达峰值的时间相同,但驯化组幼鱼血浆中的生理生化指标(WBC、Lymph、Gran、Mon、UREA、GLU-G、ALB、渗透压)和转氨酶(AST、ALT)、肝组织中的抗氧化酶(SOD、CAT、GSH-Px、MDA)上升幅度和峰值大小均显著性小于未驯化组。(3)在8 g/L NaCl盐度胁迫168 h后,驯化组和未驯化组均能够恢复到对照组水平,但驯化组的大鳞幼鱼血浆渗透压、血浆ALB含量均早于未驯化组恢复到对照组水平。研究表明,经4 g/L的盐度驯化后的大鳞鲃幼鱼遭受8 g/L NaCl盐度环境胁迫时,显示出较好的生理生化自我调节和恢复能力。