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AFLP and RAPD Analysis of the Boer and Indigenous Breeds of the Goat in Jiangsu
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作者 CAOSao-xian LUWei-zhong 《Agricultural Sciences in China》 CAS CSCD 2003年第3期344-350,共7页
Blood and tissue samples were collected from 105 goats including 60 Boer goats (30 for each sex), 30 Xuhuai goats (15 for each sex) and 15 Haimen goats (7 stud and 8 does). DNA was extracted and DNA pools were constru... Blood and tissue samples were collected from 105 goats including 60 Boer goats (30 for each sex), 30 Xuhuai goats (15 for each sex) and 15 Haimen goats (7 stud and 8 does). DNA was extracted and DNA pools were constructed on the basis of goat breeds. In 36 selective primer combinations, 29 combinations amplified totally 3 253 markers including 92 polymorphic markers by amplified fragment length polymorphism (AFLP). On average, 3.17 polymorphic markers were amplified per combination, with a polymorphic frequency of 2. 8%. The primer combinations amplifying more polymorphic markers (showed in brackets) were involved in E00+ACG/M00+CAA (13), E00+ACG/M00+CAG (10), E00+AAC/M00+CAC (8) and E00+A AC/M00+ACT (7). A total of 183 markers including 60 polymorphic markers were amplified by RAPD from the pooled DNA of three breeds using 22 primers with strong polymorphism and high reproduc-ibility selected from 93 RAPD primers. On average, 2. 73 polymorphic markers were amplified per primer, with a polymorphic frequency of 32.8%. The results of AFLP and RAPD coincidently suggested that the genetic distance is the closest between Xuhuai and Haimen goat, next between Xuhuai and Boer goat, and the farthest between Haimen and Boer goat. According to the UPGMA method, Haimen and Xuhuai goats can be gathered together as a cluster, then Boer goat. Both methods can be used to implicate the genetic difference of these three breeds, in particular AFLP has more polymorphic markers. 展开更多
关键词 Boer goat Xuhuai goat Haimen goat AFLP RAPD
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An optimized medial parapatellar approach to the goat medial femoral condyle
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作者 Pieter P.W.van Hugten Ralph M.Jeuken +2 位作者 Alex K.Roth Saskia Seeldrayers Peter J.Emans 《Animal Models and Experimental Medicine》 CSCD 2021年第1期54-58,共5页
Goats or sheep are the preferred animal model for the preclinical evaluation of cartilage repair techniques due to the similarity of the goat stifle joint to the human knee.The medial femoral condyle of the stifle joi... Goats or sheep are the preferred animal model for the preclinical evaluation of cartilage repair techniques due to the similarity of the goat stifle joint to the human knee.The medial femoral condyle of the stifle joint is the preferred site for the assessment of articular cartilage repair,as this is the primary location for this type of lesion in the human knee.Proper surgical exposure of the medial femoral condyle is paramount to obtain reproducible results without surgical error.When applying the standard human medial arthrotomy technique on the goat stifle joint,there are some key aspects to consider in order to prevent destabilization of the extensor apparatus and subsequent postoperative patellar dislocations with associated animal discomfort.This paper describes a modified surgical technique to approach the medial femoral condyle of the caprine stifle joint.The modified technique led to satisfactory exposure without postoperative incidence of patellar luxations and no long-term adverse effects on the joint. 展开更多
关键词 APPROACH arthrotomy CARTILAGE CONDYLE goats
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Analyses of circRNA profiling during the development from pre-receptive to receptive phases in the goat endometrium 被引量:10
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作者 Yuxuan Song Lei Zhang +6 位作者 Xiaorui Liu Mengxiao Niu Jiuzeng Cui Sicheng Che Yuexia Liu Xiaopeng An Binyun Cao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2019年第3期633-647,共15页
Background: Recent studies have revealed that noncoding RNAs play important regulatory roles in the formation of endometrial receptivity.Circular RNAs(circRNAs) are a universally expressed noncoding RNA species that h... Background: Recent studies have revealed that noncoding RNAs play important regulatory roles in the formation of endometrial receptivity.Circular RNAs(circRNAs) are a universally expressed noncoding RNA species that have been recently proposed to act as miRNA sponges that directly regulate expression of target genes or parental genes.Results: We used Illumina Solexa technology to analyze the expression profiles of circRNAs in the endometrium from three goats at gestational day 5(pre-receptive endometrium,PE) and three goats at gestational day 15(receptive endometrium,RE).Overall,21,813 circRNAs were identified,of which 5,925 circRNAs were specific to the RE and 9,078 were specific to the PE,which suggested high stage-specificity.Further analysis found 334 differentially expressed circRNAs in the RE compared with PE(P < 0.05).The analysis of the circRNA-miRNA interaction network further supported the idea that circRNAs act as miRNA sponges to regulate gene expression.Moreover,some circRNAs were regulated by estrogen(E2)/progesterone(P4) in endometrial epithelium cell lines(EECs) and endometrial stromal cell line(ESCs),and each circRNA molecule exhibited unique regulation characteristics with respect to E2 and P4.Conclusions: These data provide an endometrium circRNA expression atlas corresponding to the biology of the goat receptive endometrium during embryo implantation. 展开更多
关键词 CircRNAs Dairy goat ENDOMETRIAL EPItheLIUM CELLS ENDOMETRIAL stromal CELLS RECEPTIVE ENDOMETRIUM
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Assessment of Serum PRL,MLT,TSH,FSH and LH Concentrations in the Goat (Capra hircus) Exposing to Different Photoperiod Regimes 被引量:1
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作者 Huibin SHI Zhilong TIAN +5 位作者 Yuqin WANG Zhibo WU Fang YANG Jianping WANG Mingxing CHU CaiHong WEI 《Agricultural Biotechnology》 CAS 2018年第4期110-114,117,共6页
In many seasonally breeding species, photoperiod is an important factor involved in reproduction. Capra hircus breeds are normally considered short-day breeders. An experiment was carried out to evaluate whether or no... In many seasonally breeding species, photoperiod is an important factor involved in reproduction. Capra hircus breeds are normally considered short-day breeders. An experiment was carried out to evaluate whether or not photoperiodic regimens influence circulating hormone levels (prolactin, melatonin, thyrotropin stimulating hormine, luteinizing hormone,follicle stimulating hormone) of Henan huai local goat exposing to different photoperiodic regimens. The results showed that plasma levels of prolactin, melatonin, thyrotropin stimulating hormine, luteinizing hormone and follicle stimulating hormone were higher in the 8 L∶ 16 D photoperiodic regimen than that in the 16 L∶ 8 D photoperiodic regimen. Levels of those hormones decreased on day 7 and day 28 when the 16 L∶ 8 D photoperiodic regimen was used continuously. It was concluded that serum concentrations of the hormones increased in summer when short day (8 L∶ 16 D) was used and decreased when the goats were transferred from short day for 42 d to long day exposure for 28 d. We suggest that in summer, levels of hormones changing may be due to short day animals. Changing of related hormones is still determined by the mechanism of internal regulation of short day animal s exposure to light changes. Shortening light may also help increase the reproductive activity of goat and thus increase productivity. 展开更多
关键词 PHOTOPERIOD PRL MLT TSH FSH LH goat Introduction
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Expression of EGF and EGFR in Developing Skin of the Goat Fetus
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作者 QING Su-zhu,LIN Ji-mao,ZHANG Ling-zhi,WANG Lei-lei,LUO Shi-shuang (College of Veterinary Medicine,Northwest A & F University,Yangling 712100,China) 《畜牧兽医学报》 CAS CSCD 北大核心 2010年第S1期102-107,共6页
The histological characteristics,distribution and variability of epidermal growth factor(EGF) and epidermal growth factor receptor(EGFR) during development of skin in the goat fetus were studied with histological and ... The histological characteristics,distribution and variability of epidermal growth factor(EGF) and epidermal growth factor receptor(EGFR) during development of skin in the goat fetus were studied with histological and immunohistochemical methods.The results showed that the epidermis formed at the 6th gestational week,the thickness increased gradually because of transition from simple epithelium to stratified epithelium,and then decreased after the 15th week.The dermis was present at the 10th week,and the dermal derivatives appeared after the 11th week.All structures of the skin matured gradually during prenatal development.Low expression of EGF and EGFR could be observed at the 6th week and increased thereafter.Before the 11th week,EGF was mainly located in the cytoplasm of the basal layer of cells of epidermis,hair follicular epithelial cells and fibroblasts,and EGFR was mainly located on cell membranes of these cells.From the 11th to 16th week,the expression of EGF and EGFR extended from basal layer cells,prickle cells,hair follicular epithelial cells and fibroblasts to vascular endothelial cells,epithelial cells of sweat glands and arrector pili muscle.EGF was mainly detected cytoplasmically,and EGFR was located on membranes of these cells.From the 17th week to birth,EGF was mainly located in cells of the basal layer and hair follicular epithelial cells as the skin thinned and staining intensity continued to increase.The expressed amount of EGF and EGFR tended to increase throughout the whole development stage encompassed by gestational weeks 6 to 21 and the two components of the EGF signaling system were highly correlated.The results suggest that EGF and EGFR play important roles in the development of skin and its derivatives. 展开更多
关键词 EPIDERMAL GROWTH FACTor EPIDERMAL GROWTH FACTor receptor SKIN development goat
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The Relationship between Genetic Polymorphisms of Three Gene Loci and Growth Characters of Haimen Goats
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作者 李拥军 李太坤 +3 位作者 张丽 李文婷 张浩 施健飞 《Agricultural Science & Technology》 CAS 2010年第6期112-114,182,共4页
[Objective] The paper aims to study the relationship between genetic polymorphisms of three gene loci and growth character of Haimen Goats to provide reference to promote Haimen Goat growth character and cultivate Hai... [Objective] The paper aims to study the relationship between genetic polymorphisms of three gene loci and growth character of Haimen Goats to provide reference to promote Haimen Goat growth character and cultivate Haimen Goat new strain of fast-growth.[Method] We processed analysis and related statistical analysis on three gene loci of Haimen Goats with PCR-RFLP and PCR-SSCP.[Result] The results suggested that there were two genotypes in MSTN gene IntronⅡ,GH gene exonⅠ and GH gene exonⅡ in Haimen Goats.Different genotypes of these gene loci had different effects on relative growth character of Haimen Goats.[Conclusion] This study had a significant meaning about promoting Haimen Goat avoirdupois by marker assistant option. 展开更多
关键词 Haimen goats MSTN GH POLYMorPHisMS Growth character
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Effect of Moringa oleifera on semen quality profiles in endangered Teressa goat under tropical humid island ecosystem 被引量:1
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作者 P.Perumal Jai Sunder +4 位作者 A.K.De A.K.Nahak D.K.Chaurasia T.C.Balamurugan D.Bhattacharya 《Asian pacific Journal of Reproduction》 CAS 2024年第2期89-96,共8页
Objective:To investigate the effect of Moringa oleifera leaf extract on semen quality parameters in Teressa goat buck.Methods:A total of 25 semen samples from six bucks were selected for the study.Spermatozoa of 150&#... Objective:To investigate the effect of Moringa oleifera leaf extract on semen quality parameters in Teressa goat buck.Methods:A total of 25 semen samples from six bucks were selected for the study.Spermatozoa of 150×10^(6) were incubated in 0,300,500 and 700μg of leaf extract as groupⅠ,Ⅱ,Ⅲ and Ⅳ,respectively.Liquid stored semen samples were analysed for motility,viability,total sperm abnormalities,plasma membrane,acrosomal and nuclear integrities,seminal plasma intracellular enzymes[aspartate aminotransferase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)]and total antioxidant capacity(TAC)and spermatozoa malondialdehyde(MDA)and cholesterol efflux in comparison with the control group(groupⅠ)for up to 72 h.Results:Moringa oleifera leaf extract(500μg/150×10^(6) spermatozoa)-treated semen had significantly higher motility,viability,plasma membrane,acrosomal and nuclear integrities,and TAC(P<0.05),and had significantly lower total sperm abnormalities,AST,ALT,LDH,MDA production and cholesterol efflux compared to those in other Moringa oleifera leaf extract treated(300 or 700μg/150×10^(6) spermatozoa)and control groups at different hours of liquid storage(P<0.05).Semen quality parameters and TAC showed an increasing trend and total sperm abnormalities,MDA production,leakage of intra-cellular enzymes and cholesterol efflux showed a decreasing trend from group Ⅰto group Ⅲand then an opposite trend from group Ⅲ to group Ⅳ at different hours of liquid storage.Conclusions:Moringa oleifera leaf extract 500μg/150×10^(6) spermatozoa can be used for liquid semen preservation in Teressa goat at Andaman and Nicobar Islands. 展开更多
关键词 Moringa oleifera SEMEN Teressa goat Andaman and Nicobar islands
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Etiological Agent, Pathogenesis, Diagnosis, Treatment, Measures for Prevention and Control of Caseous Lymphadenitis Disease in the Small Ruminants with Special Reference to Sheep
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作者 Eréndira de la Fuente Mancera Abel Ciprian Carrasco Susana Mendoza Elvira 《Journal of Biosciences and Medicines》 2024年第5期154-170,共17页
Caseous lymphadenitis (CL) is a chronic infectious disease caused by Corynebacterium pseudotuberculosis (C. pseudotuberculosis) that is transmitted mainly by contact with contaminated exudate wounds. Caseous lymphaden... Caseous lymphadenitis (CL) is a chronic infectious disease caused by Corynebacterium pseudotuberculosis (C. pseudotuberculosis) that is transmitted mainly by contact with contaminated exudate wounds. Caseous lymphadenitis affects different species of animals and is considered an occupational zoonotic disease. CL is responsible for important economic losses in the small ruminant industry, which include decreased production, damaged quality of milk and wool, reproductive disorders, total or partial confiscation of carcasses, and depreciation of the skin. Caseous lymphadenitis disease can present in two clinical pictures: a skin or superficial picture and a visceral or deep picture. The presumptive diagnosis of CL in the skin is based on the exploration of superficial lymph nodes. These have little value in diagnosing visceral CL, where the main sign is emaciation. The prevention and control of CL involve identifying the skin condition and debriding the abscesses. In addition to the treatment of superficial lesions, the animal facilities must be repaired and adapted. To avoid exposure of negative herds to CL, new animals must be subjected to observation to identify any of the clinical manifestations. Another form of control is vaccination, though only some countries have commercial vaccines. General information on the etiological agent and its characteristics can be used to improve the understanding of this disease. 展开更多
关键词 ABSCESS CMNR Corynebacterium pseudotuberculosis goat CL Lymph Nodes PSEUDOTUBERCULOSis SHEEP
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Cysticercus Tenuicollis in Visceral Organs of Goats and Sheep in Uganda: A Case Study in Lira Municipal Abattoir
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作者 Joseph Magala Barnabas Mudde +4 位作者 Patrick Abel Mawadri Williams Guma Sylvester Dickson Baguma Patrick Olango Okot Jolly Akullo 《Open Journal of Animal Sciences》 2024年第4期249-259,共11页
Cysticercus tenuicollis is a metacestode stage of Taenia hydatigena residing in the visceral organs of small ruminants and its effects cause economic losses due to condemnation of visceral organs in slaughterhouses. T... Cysticercus tenuicollis is a metacestode stage of Taenia hydatigena residing in the visceral organs of small ruminants and its effects cause economic losses due to condemnation of visceral organs in slaughterhouses. This study reports on the prevalence of Cysticercus tenuicollis in small ruminants slaughtered in Lira Municipal Abattoir. A total of 70 sheep and 70 goats were sampled and examined after slaughter for the presence of Cysticercus tenuicollis in the visceral organs using standard meat inspection procedures. An abattoir data collection sheet was used to record data, which was then analysed in SPSS version 20. The study revealed 51.4% of goats and 68.6% of sheep to be infested with Cysticercus tenuicollis. Following the district of origin for the small ruminants, Otuke district had the highest prevalence of the parasite (82.6%), followed by Kole (72.4%), Alebtong (66.7%), Apac (59.4%) and Lira (31.4%). Cysticercus tenuicollis was more frequently seen in the intestines (57.8%) of sheep and goats than in any other visceral organs. The liver had a prevalence of 20.7%, the lungs 17.8%, the kidneys 3.5% and the fetal sac;(15.7%) in goats and (12.9%) in sheep. Cysticercus tenuicollis was detected in 68.8% of females and 58.8% of male small ruminants. It is recommended to routinely deworm goats and sheep by farmers most especially in districts with the highest prevalence of Cysticercus tenuicollis infestation. The sale of the condemned parts of sheep and goats and the feeding of dogs on these contaminated materials should be stopped forthwith. It is also being suggested that a national study be carried out to determine the prevalence and economic impact of Cysticercus tenuicollis in small ruminants. 展开更多
关键词 Cysticercus tenuicollis goats and Sheep ABATTOIR
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circRNA3669 promotes goat endometrial epithelial cells proliferation via miR-26a/RCN2 to activate PI3K/AKT-mTOR and MAPK pathways
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作者 Xiaorui Liu Jiuzeng Cui +8 位作者 Mengyao Wei Xiaofei Wang Yuexia Liu Zhongshi Zhu Min Zhou Gui Ba Langda Suo Yuxuan Song Lei Zhang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第3期960-974,共15页
The development of receptive endometrium(RE) from pre-receptive endometrium(PE) for successful embryo implantation is a complex dynamic process in which the morphology and physiological states of the endometrial epith... The development of receptive endometrium(RE) from pre-receptive endometrium(PE) for successful embryo implantation is a complex dynamic process in which the morphology and physiological states of the endometrial epithelium undergo a series of significant changes, including cell proliferation and apoptosis. However, the molecular mechanisms are not yet fully understood. In this study, a higher circRNA3669 level was observed in PE than in RE of goats. Functional assays revealed that this overexpression promoted the proliferation of goat endometrial epithelial cells(GEECs) by activating the expression of genes related to the PI3K/AKT-mTOR and MAPK pathways,thereby inhibiting apoptosis in vitro. Furthermore, circRNA3669 functioned as a competing endogenous RNA(ceRNA) to upregulate Reticulocalbin-2(RCN2) expression at the post-transcriptional level by interacting with and downregulating miR-26a in GEECs. In addition, RCN2, which is highly expressed in the PE of goats, was found to be regulated by β-estradiol(E2) and progesterone(P4). Our results demonstrated that RCN2 also affected the key proteins PI3K, AKT, mTOR, JNK, and P38 in the PI3K/AKT-mTOR and MAPK pathways, thereby facilitating GEECs proliferation and suppressing their apoptosis in vitro. Collectively, we constructed a new circRNA3669-miR-26aRCN2 regulatory network in GEECs, which further provides strong evidence that circRNA could potentially play a crucial regulatory role in the development of RE in goats. 展开更多
关键词 circRNA3669 RCN2 miR-26a goat endometrial epithelial cells(GEECs) PROLIFERATION
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DNA Polymorphisms of 5′-Flanking Region of Insulin-Like Growth Factor 1 Gene and Their Association with Reproduction Traits in Goats 被引量:4
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作者 WANG Ping-qing TAN Ying +4 位作者 ZHANG Bao-yun CHU Ming-xing DENG La-mei FAN Qi LIUChong-xu 《Agricultural Sciences in China》 CAS CSCD 2011年第10期1609-1617,共9页
Research on the identity of genes and their relationship with traits of economic importance in farm animals could assist in the selection of livestock. In this study, the polymorphisms of insulin-like growth factor 1 ... Research on the identity of genes and their relationship with traits of economic importance in farm animals could assist in the selection of livestock. In this study, the polymorphisms of insulin-like growth factor 1 (IGF1) gene in 561 goats of ten breeds were detected by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) and their association with litter size and birth weight in three breeds were investigated. The effects of IGF1 polymorphisms on the breeding value for litter size and birth weight were examined using least square methods. Two deletions (CA) were detected in the microsatellite and two mutations (A1637G, T1640C) were found in 5′-flanking regulatory region. No significant association between the polymorphisms in 5′-flanking region of IGF1 and birth weight was found in the three breeds of goats. In Gulin Ma goats, two polymorphisms were found to affect litter size traits. In Chuandong White goats and Guizhou White goats, no significant difference (P0.05) in litter size between goats carrying different genotypes was observed. Further evaluation and confirmation studies in more goat populations with larger sample sizes are necessary. 展开更多
关键词 IGF1 gene DNA polymorphism litter size birth weight goat
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Relationship between the Contents of MDA, the Activity of SOD in Serum of the Milk Goat with Fluorosis and the Erythrocytic Membrane Liquidity 被引量:1
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作者 LI Shu GUO Tie-hong +1 位作者 XU Shi-wen ZHU Fei 《Journal of Northeast Agricultural University(English Edition)》 CAS 2005年第2期118-121,共4页
To research the relation among the erythrocytic membrane.liquidity and the contents of MDA, the activity of SOD in serum of the milk goat with fluorosis. An animal model with fluorosis was constructed, and the fluores... To research the relation among the erythrocytic membrane.liquidity and the contents of MDA, the activity of SOD in serum of the milk goat with fluorosis. An animal model with fluorosis was constructed, and the fluorescent probe technique of DPH was used to measue the erythrocytic membrane liquidity. At the same time, the contents of MDA and the activity of SOD in serum were measured. The results showed that the erythrocytic membrane liquidity in the control group and flurosis group were 5.6742 ± 0.4417 and 3.7248 ± 0.4521 (P 〈0.01) respectively, the contents of MDA in serum were 2.0408 ± 0.198 and 4.494± 0.438 (P 〈0.01) respectively, the activities of SOD were 175.638 ± 22.201 and 113.714 ± 34.258 (P 〈0.01) respectively. The correlation analysis indicated that the relation between the activity of SOD and the liquidity of erythrocytic membrane was positive correlation ( r= 0.7321, P 〈0.05), whereas the relation between the contents of MDA and the liquidity of erythrocytic membrane was negative correlation (r = -0.6438, P 〈0.01). The erythrocytic membrane liquidity decreased in milk goat with fluorosis, which played a role in the occurrence and development of the fluorosis. There was correlation among the erythrocytic membrane liquidity and the contents of MDA, the activity of SOD. 展开更多
关键词 milk goat FLUorOSis erythrocytic membrane
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Comparative proteomic analysis of the effects of high-concentrate diet on the hepatic metabolism and inflammatory response in lactating dairy goats 被引量:3
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作者 Yongqian Duanmu Rihua Cong +5 位作者 Shiyu Tao Jing Tian Haibo Dong Yuanshu Zhang Yingdong Ni Ruqian Zhao 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2016年第2期217-227,共11页
Background: To understand the impact of feeding a high-concentrate diet to mid-lactating goats for a long time on liver metabolism and inflammatory response, two dimensional polyacrylamide gel electrophoresis(2-DE)... Background: To understand the impact of feeding a high-concentrate diet to mid-lactating goats for a long time on liver metabolism and inflammatory response, two dimensional polyacrylamide gel electrophoresis(2-DE) and real-time PCR method were employed to detect proteins differentially expressed in liver and their m RNAs expression in goats fed high concentrate diet(HC) or low concentrate diet(LC). Twelve lactating dairy goats were randomly assigned to either a HC diet group(65 % concentrate of dry matter; n = 6) or a LC diet group(35 % concentrate of dry matter; n = 6) for 10 wk.Results: Twenty differentially expressed spots(≥2.0-fold changes) in the hepatic tissues were excised and successfully identified using MALDI TOF/TOF. Of these, 8 proteins were up-regulated, while the rest 12 proteins were down-regulated in HC goats compared to LC. Differential expressed proteins including alpha enolase 1(ENO1), glutamate dehydrogenase 1(GLUD1), glutathione S-transferase A1(GSTA1), ATP synthase subunit 5β(ATP5β), superoxide dismutase [Cu-Zn](SOD1), cytochrom c oxidase subunit Via(COX6A1) and heat shock protein 60(HSP60) were further verified by real-time PCR and/or western blot at m RNA or protein expression level. Consistent with the 2-DE results, a significant decrease of β-actin protein expression and SOD enzyme activity was observed in liver of HC goats(P 〈 0.05), while ENO1 protein expression was significantly up-regulated in HC compared to LC goats(P 〈 0.05). However, western blot analysis did not show a significant difference of hepatic HSP60 protein between HC and LC group, which did not match the decrease of HSP60 content detected by 2-DE analysis. Real-time PCR showed that glutathione S-transferase P1(GSTP1) and SOD1 m RNA expression was significantly decreased in liver of HC goats, while cytochrom c oxidase(COX3) and ATPase 8(ATP8) m RNAs expression were markedly increased compared to LC(P 〈 0.05). Gene Ontology(GO) analysis revealed that HC diet resulted in altered expression of proteins related to catalytic and mitochondrial metabolism in the liver, and may increase the stress response with up-regulating the expression of differentiation 14(CD14) cluster and serum amyloid A(SAA) as well as C-reactive protein(CRP) in the liver.Conclusions: These results suggest that feeding high concentrate diet to lactating goats for 10 wk leads to the activation of the inflammatory response, and decreases the anti-oxidant capacity, and subsequently impairs the mitochondrial function in the liver. 展开更多
关键词 High concentrate diet Lactating goats Liver Mitochondria Proteomics
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Immunostimulatory Effect of Levamisole on the Immune Response of Goats to <i>Peste des petits ruminants </i>(PPR) Vaccination 被引量:1
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作者 Undiandeye Jude Undiandeye Bamidele Soji Oderinde +1 位作者 Abduldahiru El-Yuguda Saka Saheed Baba 《World Journal of Vaccines》 2014年第2期88-95,共8页
The use of Levamisole hydrochloride as an immunostimulant has been successful in the control of certain diseases such as Derzsy’s disease in chicken as well as in increasing lymphocytes and weight gain in pigs. Howev... The use of Levamisole hydrochloride as an immunostimulant has been successful in the control of certain diseases such as Derzsy’s disease in chicken as well as in increasing lymphocytes and weight gain in pigs. However, its use as immunostimulant in the prevention of Peste des petits ruminants (PPR) in goats has not been investigated. In this study, the use of Levamisole in enhancing immune response to PPR vaccination including its effects on weight gain was investigated among groups of goats (groups A, B, C and D). Virus neutralization test was used to determine the antibody profile in vaccinated goats while the total and differential leucocyte counts and body mass index (BMI) were determined using standard procedures. Goats in group A (Levamisole primed and PPR vaccinated) seroconverted to more than 3-folds of the initial pre-vaccination geometric mean titre (GMT) of neutralizing antibody beginning from second week post-vaccination and remained high throughout the period of the experiment. Similarly, there was significant increase (p 0.05) in BMI of animals in other groups throughout the period of the experiment. The results of this study further confirm the immunostimulatory effect of Levamisole when used in combination with a vaccine. 展开更多
关键词 LEVAMisOLE Hydrochloride Homologous PPR Vaccine IMMUNOSTIMULANT goats Maiduguri Nigeria
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Sulforaphane prevents LPS‑induced inflammation by regulating the Nrf2‑mediated autophagy pathway in goat mammary epithelial cells and a mouse model of mastitis 被引量:1
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作者 Dan Shao Wenxiang Shen +6 位作者 Yuyang Miao Zhen Gao Menghao Pan Qiang Wei Zuoting Yan Xiaoe Zhao Baohua Ma 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第5期2093-2106,共14页
Background Mastitis not only deteriorates the composition or quality of milk,but also damages the health and pro-ductivity of dairy goats.Sulforaphane(SFN)is a phytochemical isothiocyanate compound with various pharma... Background Mastitis not only deteriorates the composition or quality of milk,but also damages the health and pro-ductivity of dairy goats.Sulforaphane(SFN)is a phytochemical isothiocyanate compound with various pharmacologi-cal effects such as anti-oxidant and anti-inflammatory.However,the effect of SFN on mastitis has yet to be elucidated.This study aimed to explore the anti-oxidant and anti-inflammatory effects and potential molecular mechanisms of SFN in lipopolysaccharide(LPS)-induced primary goat mammary epithelial cells(GMECs)and a mouse model of mastitis.Results In vitro,SFN downregulated the mRNA expression of inflammatory factors(tumor necrosis factor-α(TNF-α),interleukin(IL)-1βand IL-6),inhibited the protein expression of inflammatory mediators(cyclooxygenase-2(COX2),and inducible nitric oxide synthase(iNOS))while suppressing nuclear factor kappa-B(NF-κB)activation in LPS-induced GMECs.Additionally,SFN exhibited an antioxidant effect by increasing Nrf2 expression and nuclear translocation,up-regulating antioxidant enzymes expression,and decreasing LPS-induced reactive oxygen species(ROS)produc-tion in GMECs.Furthermore,SFN pretreatment promoted the autophagy pathway,which was dependent on the increased Nrf2 level,and contributed significantly to the improved LPS-induced oxidative stress and inflammatory response.In vivo,SFN effectively alleviated histopathological lesions,suppressed the expression of inflammatory factors,enhanced immunohistochemistry staining of Nrf2,and amplified of LC3 puncta LPS-induced mastitis in mice.Mechanically,the in vitro and in vivo study showed that the anti-inflammatory and anti-oxidative stress effects of SFN were mediated by the Nrf2-mediated autophagy pathway in GMECs and a mouse model of mastitis.Conclusions These results indicate that the natural compound SFN has a preventive effect on LPS-induced inflam-mation through by regulating the Nrf2-mediated autophagy pathway in primary goat mammary epithelial cells and a mouse model of mastitis,which may improve prevention strategies for mastitis in dairy goats. 展开更多
关键词 AUTOPHAGY goat mammary epithelial cells INFLAMMATION NRF2 Oxidative stress SULForAPHANE
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Whole-genome methylation analysis reveals epigenetic variation between wild-type and nontransgenic cloned,ASMT transgenic cloned dairy goats generated by the somatic cell nuclear transfer 被引量:1
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作者 Hao Wu Wendi Zhou +10 位作者 Haijun Liu Xudai Cui Wenkui Ma Haixin Wu Guangdong Li Likai Wang Jinlong Zhang Xiaosheng Zhang Pengyun Ji Zhengxing Lian Guoshi Liu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第1期98-113,共16页
Background:SCNT(somatic cell nuclear transfer)is of great significance to biological research and also to the livestock breeding.However,the survival rate of the SCNT cloned animals is relatively low compared to other... Background:SCNT(somatic cell nuclear transfer)is of great significance to biological research and also to the livestock breeding.However,the survival rate of the SCNT cloned animals is relatively low compared to other transgenic methods.This indicates the potential epigenetic variations between them.DNA methylation is a key marker of mammalian epigenetics and its alterations will lead to phenotypic differences.In this study,ASMT(acetylserotonin-Omethyltransferase)ovarian overexpression transgenic goat was produced by using SCNT.To investigate whether there are epigenetic differences between cloned and WT(wild type)goats,WGBS(whole-genome bisulfite sequencing)was used to measure the whole-genome methylation of these animals.Results:It is observed that the different m Cp G sites are mainly present in the intergenic and intronic regions between cloned and WT animals,and their CG-type methylation sites are strongly correlated.DMR(differentially methylated region)lengths are located around 1000 bp,mainly distributed in the exonic,intergenic and intronic functional domains.A total of 56 and 36 DMGs(differentially methylated genes)were identified by GO and KEGG databases,respectively.Functional annotation showed that DMGs were enriched in biological-process,cellularcomponent,molecular-function and other signaling pathways.A total of 10 identical genes related to growth and development were identified in GO and KEGG databases.Conclusion:The differences in methylation genes among the tested animals have been identified.A total of 10 DMGs associated with growth and development were identified between cloned and WT animals.The results indicate that the differential patterns of DNA methylation between the cloned and WT goats are probably caused by the SCNT.These novel observations will help us to further identify the unveiled mechanisms of somatic cell cloning technology,particularly in goats. 展开更多
关键词 Acetylserotonin-O-methyltransferase Dairy goat DNA methylation Gene editing Somatic cell nuclear transfer
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Potential Genes for Regulation of Milk Protein Synthesis in Dairy Goat Mammary Gland
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作者 Chen Dan Zhang Na +2 位作者 Nan Xue-mei Li Qing-zhang Gao Xue-jun 《Journal of Northeast Agricultural University(English Edition)》 CAS 2016年第1期50-56,共7页
The lactating mammary gland is a prodigious protein-producing factory, but the milk protein synthesis mechanisms are not well understood. The major objective of this paper was to elucidate which genes and pathways wer... The lactating mammary gland is a prodigious protein-producing factory, but the milk protein synthesis mechanisms are not well understood. The major objective of this paper was to elucidate which genes and pathways were involved in the regulation of milk protein synthesis in the dairy goat mammary gland. Total 36 primiparous Guanzhong dairy goats were allotted in 12 groups according to their mammary development stages: days 90 and 150 of virgin, days 30, 90, and 150 of pregnancy, days 1, 10, 35, and 60 of lactation and days 3, 7, and 21 of involution (three animals per group). Mammary tissue RNA was isolated for quantitative real- time RT-PCR of four casein genes alpha-s 1 casein (CSN 1S 1 ), alpha-s2 casein (CSN 1 S2), beta-casein (CSN2) and casein kappa (CSN3), four whey protein genes lactoglobulin (LGB), laetalbumin (LALBA), laetofarrin (LTF), and Whey acidic protein (WAP) and the genes which were potentially to regulate dairy goat milk protein synthesis at the level of transcription or translation [prolactin receptor (PRLR), AKT1, signal transducers and activators of transcription 5 (STAT5), E74-Like Factor 5 (ELF5), eukaryotic translation initiation factor 4E binding protein 1 (EIF4E-BP1), S6kinase (S6K) and caveolin 1]. The results showed that all genes were up-regulated in lactation period. The expressions of PRLR, AKT1, STAT5, ELF5, and S6K were similar to mRNA expressions of milk proteins. Our results indicated that milk protein synthesis in dairy goat mammary gland was possibly regulated by these genes. 展开更多
关键词 protein synthesis mammary gland dairy goat
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Influence of Additional Feeding of Sucrose Goat Females on Indicators of Natural Resistance of the Body and Productivity of Kids
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作者 Zayniddin Rajamuradov Nuriniso Rajamuradova 《Open Journal of Animal Sciences》 CAS 2022年第3期506-514,共9页
The traditional way of breeding pastured animals is year-round pasture keeping without any additional fertilizing. Recently, among adult animals and the resulting offspring, a large number of individuals with low leve... The traditional way of breeding pastured animals is year-round pasture keeping without any additional fertilizing. Recently, among adult animals and the resulting offspring, a large number of individuals with low levels of natural resistance have begun to appear, which negatively affects the productivity of adults, and growth and development, as well as the safety of the resulting offspring. In this regard, the aim of the study was to study the effects of full-fledged feeding of females during pregnancy on the level of natural resistance of the received goats. To achieve this goal, a number of modern physiological and biochemical research methods were used. According to the results obtained, it was established that with the organization of full-fledged feeding, the period of suckling of the goat develops normally and the resulting baby goats are born with increased levels of natural resistance and more massive. In the goats of the experimental group, these advantages persist steadily until the age of one year, and to a certain extent ensure the normal preservation of the received goats and their growth and development. This makes it possible for farmers to fatten goats unused in breeding from 8 - 9 months of age and donate them for meat as an additional economic supplement to production. 展开更多
关键词 goats goats Natural Resistance of the Body NUTRIENTS Security Durability
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Novel intron 2 polymorphism in the melanophilin gene is in Hardy-Weinberg equilibrium and is not associated with coat color in goats
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作者 Mufliat A. Adefenwa Brilliant O. Agaviezor +9 位作者 Sunday O. Peters Matthew Wheto Oludotun J. Ekundayo Moses Okpeku Bola O. Oboh Khalid O. Adekoya Christian O. N. Ikeobi Marcos De Donato Bolaji N. Thomas Ikhide G. Imumorin 《Open Journal of Genetics》 2013年第3期195-200,共6页
Pigmentation plays important adaptation and physiological efficiency roles in animals. In the sequence of a 648 bp fragment representing intron 1, exon 2, and part of intron 2 of the MLPH mammalian pigmentation gene, ... Pigmentation plays important adaptation and physiological efficiency roles in animals. In the sequence of a 648 bp fragment representing intron 1, exon 2, and part of intron 2 of the MLPH mammalian pigmentation gene, we identified a novel g.469C> G mutation in intron 2, and genotyped it in 266 Nigerian goats using PCR-RFLP analysis. The C allele had frequencies of 0.9625, 0.9804 and 0.97405 in West African Dwarf (WAD),Sahel(SH) and Red Sokoto (RS) breeds, respectively. The G allele was the highest in WAD (0.0375), followed by RS (0.02595), and then SH (0.0196). Overall low FIS and FST and high Nm values demonstrate little differentiation within and among the goat breeds at this intronic locus. This g.469C> G polymorphism in MLPH gene is the first in any goat breed and also first in Nigerian goats. Our results suggest that this intronic SNP locus is maintained at Hardy-Weinberg equilibrium (P < 0.05) and the lack of association of this SNP with coat color may indicate its neutrality in goats. 展开更多
关键词 COAT Color goats MELANOPHILIN Nigeria INTRON 2 SNP PCR-RFLP
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Comparison the Genetic Potential of Some Growth & Milk Trait, s of Hybrid (Crossed) Saanen with Native Goat (Lori Black Goat) versus Native Goats in Qom Province
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作者 S. M. Hoseini Ghazikhani M. Kalantar 《Journal of Agricultural Science and Technology(A)》 2011年第8期1297-1299,共3页
The performance of some growth and milk traits of selected Qomi native (Lori black) goats and hybrids of saanen with native goats during 2008-2010 was compared. Growth traits include birth weight (BW), one-month w... The performance of some growth and milk traits of selected Qomi native (Lori black) goats and hybrids of saanen with native goats during 2008-2010 was compared. Growth traits include birth weight (BW), one-month weight (1 MW), weaning weight (WW) and 6-month weight (6 MW). Also the milk production traits was monthly milk production on 2, 3, 4, 5th months (quantitative traits) and some milk composition included dry matter (%DM), fat (%F), and protein (%P) percentage on during of production (qualitative traits). There were no significant differences in sex, birth type and genetic group for BW. Also the effect of genetic groups on 1 MW, WW, and 6 MW was significant, while effects of sex and birth type on 1 MW, WW and 6 MW was not significant. Also MW (Mothe's weight) had no significant effect on BW, 1 MW, WW and 6 MW. The average of daily milk records was 0.48 kg for nativel and 1.1 kg for crossed goats. The maximum monthly milk records in two groups were in second month (native: 0.64 -4- 0.05, crossed: 1.44 ~: 0.12) and the minimum were in fifth month (native: 0.36 + 0.03, crossed: 0.77 -4- 0.05). %DM, %F and %P in 5th month were higher than other months in two groups. Effects of genetic groups on milk production and also on average of%F, %DM and %P were significant. This study showed that the crossbreeding has a significant effect on most milk production and growth traits in Iranian native goats. 展开更多
关键词 Saanen goat qomi native goat growth traits milk traits
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