Exosome-like small-extracellular vesicles (sEVs) are extracellular vesicles that act in intercellular communication and are involved in several biologic and pathologic processes. While sEVs increase the stability of t...Exosome-like small-extracellular vesicles (sEVs) are extracellular vesicles that act in intercellular communication and are involved in several biologic and pathologic processes. While sEVs increase the stability of their cargo molecules, there is still a need for standardization of sampling and isolation of these microvesicles. We aimed to determine the best sampling method for isolation of sEVs from peripheral blood from reproductive-aged women. Material and Methods: We included samples of plasma from our biobank collected in 2014 by venipuncture in heparin tubes and stored at -80°C. We also included blood samples collected in heparin tubes and Ethylenediamine tetraacetic acid (EDTA) tubes and stored at -80°C for one to two weeks prior processing. All blood samples were collected from the same nine reproductive-aged female volunteers. sEVs were isolated from plasma by ultracentrifugation and filtration and indirectly quantified using Pierce BCA Protein Assay kit. Transmission electron microscopy (TEM) and Nano Tracking Analysis (NTA) were performed to confirm the isolation of sEVs. Results and Discussion: TEM and NTA confirmed the isolation of sEVs. Protein concentration of short-time stored heparin samples was not statistically different from long-time stored heparin samples (1847.2 ± 651.4 vs. 2363.2 ± 1025.1, p = 0.14). There was no difference between heparin and EDTA plasma samples recently collected (2363.2 ± 1025.1 vs. 2044.8 ± 653.2, p = 0.44). In conclusion, blood samples may be collected using heparin or EDTA for isolation of sEVs. Long-time stored plasma samples maintain sEVs integrity and may be used, especially in comparative studies.展开更多
目的分析高龄孕妇介入性产前诊断胎儿染色体异常结果的特征。方法回顾性选取2020年1月至2023年6月于唐山市妇幼保健院产前诊断遗传病诊断中心就诊的行羊膜腔穿刺术的638例高龄孕妇作为研究对象,按照孕妇预产年龄分为A组(35~<40岁,n=4...目的分析高龄孕妇介入性产前诊断胎儿染色体异常结果的特征。方法回顾性选取2020年1月至2023年6月于唐山市妇幼保健院产前诊断遗传病诊断中心就诊的行羊膜腔穿刺术的638例高龄孕妇作为研究对象,按照孕妇预产年龄分为A组(35~<40岁,n=463)和B组(≥40岁,n=175),统计2组高龄孕妇羊水细胞染色体核型分析结果和全基因组拷贝数变异测序(copy number variation sequencing,CNV-seq)检测结果。统计学方法采用χ^(2)检验。结果638例高龄孕妇中,羊水细胞染色体异常核型检出率为8.3%(53/638),其中A组和B组的检出率分别为6.9%(32/463)和12.0%(21/175),B组高于A组(χ^(2)=15.241,P<0.05)。CNV-seq检测结果显示,羊水细胞染色体异常拷贝数变异(copy number variation,CNV)检出率为10.2%(65/638),其中A组和B组的检出率分别为8.9%(41/463)和13.7%(24/175),B组高于A组(χ^(2)=13.634,P<0.05)。结论在高龄孕妇中,胎儿染色体异常发生率随着孕妇年龄增长而上升,行产前诊断羊水细胞染色体核型分析及CNV-seq检测可提高胎儿染色体遗传病的检出率。展开更多
Background：Quantifying syphilis prevalence is important for planning interventions and advocating for resources on syphilis.However,data on large sample studies regarding the prevalence of syphilis among reproductive...Background：Quantifying syphilis prevalence is important for planning interventions and advocating for resources on syphilis.However,data on large sample studies regarding the prevalence of syphilis among reproductive-age women in rural China were not available for analysis.The aim of the study was to determine the prevalence,epidemiological characteristics,and related factors of syphilis infection among reproductive-age women in rural China.Methods：Data were obtained from a nationwide,population-based,cross-sectional study under the National Free Preconception Health Examination Project which covered all the 31 provinces in China's Mainland.Women intending to get pregnant within the next 6 months were enrolled between January 1,2010,and December 31,2012.Sociodemographic,gynecological and obstetric characteristics,and other relevant information were obtained through face-to-face interviews.Treponema pallidum particle agglutination assay test was used to detect positive samples of syphilis.Univariate and multivariate logistic regressions were performed to assess the associations between syphilis seropositivity and related factors.Results：The overall seroprevalence of syphilis （SPS） among the 2,044,126 women who received syphilis screening test during 2010-2012 was 0.37% （95% confidence interval [CI]：0.36-0.37%）.The SPS appeared 0.24% （95% CI：0.23-0.26%） and 0.66% （95% CI：0.59-0.72%） in women at 21-24 and 40-44 years of age,respectively,showing an increase of SPS,parallel with age,and the difference was significant.SPS was significantly higher in ethnic minorities than that in Han nationality （0.58% vs.0.35%,respectively,odds ratio [OR] =1.41,95% CI：1.30-1.53） and higher in workers than that in farmers （0.45% vs.0.36%,respectively,OR =1.27,95% CI：1.14-1.41）.Women with primary school or below level had a higher SPS as compared to those with college or above educational level （0.61% vs.0.32%,respectively,OR =2.49,95% CI：2.14-2.89）,and the increase reversely correlated with the levels of education.Women whose spouses were syphilis seropositive had significant greater risk （OR =48.26,95% CI：44.38-52.48） as compared those whose spouses were seronegative.Women who reported having had a history of sexually transmitted infections were more likely to be tested positive for serological syphilis （OR =27.17,95% CI：20.44-36.11） as compared to those without.Conclusions：High SPS is seen among reproductive-age women in rural China that calls for targeted interventions on syphilis prevention and control in this target population,with emphasis on those who are 35 years of age and above,less educated,being minor ethnicity,workers,and living in the western regions of China.展开更多
目的分析329例孕妇血小板抗体阳性结果,探讨血小板抗体与妊娠次数和流产史的关系。方法选取2017年1月—2021年12月厦门大学附属第一医院329例住院孕妇为研究对象,采用固相凝集法进行血小板抗体筛查,分析孕妇血小板抗体阳性率与年龄、妊...目的分析329例孕妇血小板抗体阳性结果,探讨血小板抗体与妊娠次数和流产史的关系。方法选取2017年1月—2021年12月厦门大学附属第一医院329例住院孕妇为研究对象,采用固相凝集法进行血小板抗体筛查,分析孕妇血小板抗体阳性率与年龄、妊娠次数和流产史等因素之间的关系。结果329例孕妇中,血小板抗体阳性50例,阳性率15.198%;经产妇血小板抗体阳性率(19.431%)高于初产妇(7.627%),差异具有统计学意义(P<0.05),妊娠次数不同阳性率不同(1次7.627%,2次17.582%,>2次20.833%),三者差异具有统计学意义(P<0.05),组间趋势χ^(2)检验显示妊娠次数和血小板抗体有线性趋势(P<0.05);有流产史的孕妇抗体阳性率(23.529%)大于无流产史(13.027%),二者差异具有统计学意义(P<0.05);年龄对孕妇血小板抗体阳性率无影响(P>0.05)。结论孕妇妊娠次数和流产史与血小板阳性率密切相关,妊娠孕妇应该进行血小板抗体筛查,特别是有妊娠史和流产史的孕妇更应重视,这对于预防和减少孕期流产和胎儿/新生儿同种异体免疫血小板减少症(fetal and neonatal alloimmune thrombocytopenia,FNAIT)具有重要的临床意义。展开更多
基金We acknowledge the volunteers that had donated blood samples for this experi-ment.This work was supported by Sao Paulo Research Foundation(Fapesp)(grants 2016/13616-8,2016/16618-1,2016/01340-8 and 2015/21829-9).
文摘Exosome-like small-extracellular vesicles (sEVs) are extracellular vesicles that act in intercellular communication and are involved in several biologic and pathologic processes. While sEVs increase the stability of their cargo molecules, there is still a need for standardization of sampling and isolation of these microvesicles. We aimed to determine the best sampling method for isolation of sEVs from peripheral blood from reproductive-aged women. Material and Methods: We included samples of plasma from our biobank collected in 2014 by venipuncture in heparin tubes and stored at -80°C. We also included blood samples collected in heparin tubes and Ethylenediamine tetraacetic acid (EDTA) tubes and stored at -80°C for one to two weeks prior processing. All blood samples were collected from the same nine reproductive-aged female volunteers. sEVs were isolated from plasma by ultracentrifugation and filtration and indirectly quantified using Pierce BCA Protein Assay kit. Transmission electron microscopy (TEM) and Nano Tracking Analysis (NTA) were performed to confirm the isolation of sEVs. Results and Discussion: TEM and NTA confirmed the isolation of sEVs. Protein concentration of short-time stored heparin samples was not statistically different from long-time stored heparin samples (1847.2 ± 651.4 vs. 2363.2 ± 1025.1, p = 0.14). There was no difference between heparin and EDTA plasma samples recently collected (2363.2 ± 1025.1 vs. 2044.8 ± 653.2, p = 0.44). In conclusion, blood samples may be collected using heparin or EDTA for isolation of sEVs. Long-time stored plasma samples maintain sEVs integrity and may be used, especially in comparative studies.
文摘目的分析高龄孕妇介入性产前诊断胎儿染色体异常结果的特征。方法回顾性选取2020年1月至2023年6月于唐山市妇幼保健院产前诊断遗传病诊断中心就诊的行羊膜腔穿刺术的638例高龄孕妇作为研究对象,按照孕妇预产年龄分为A组(35~<40岁,n=463)和B组(≥40岁,n=175),统计2组高龄孕妇羊水细胞染色体核型分析结果和全基因组拷贝数变异测序(copy number variation sequencing,CNV-seq)检测结果。统计学方法采用χ^(2)检验。结果638例高龄孕妇中,羊水细胞染色体异常核型检出率为8.3%(53/638),其中A组和B组的检出率分别为6.9%(32/463)和12.0%(21/175),B组高于A组(χ^(2)=15.241,P<0.05)。CNV-seq检测结果显示,羊水细胞染色体异常拷贝数变异(copy number variation,CNV)检出率为10.2%(65/638),其中A组和B组的检出率分别为8.9%(41/463)和13.7%(24/175),B组高于A组(χ^(2)=13.634,P<0.05)。结论在高龄孕妇中,胎儿染色体异常发生率随着孕妇年龄增长而上升,行产前诊断羊水细胞染色体核型分析及CNV-seq检测可提高胎儿染色体遗传病的检出率。
文摘Background：Quantifying syphilis prevalence is important for planning interventions and advocating for resources on syphilis.However,data on large sample studies regarding the prevalence of syphilis among reproductive-age women in rural China were not available for analysis.The aim of the study was to determine the prevalence,epidemiological characteristics,and related factors of syphilis infection among reproductive-age women in rural China.Methods：Data were obtained from a nationwide,population-based,cross-sectional study under the National Free Preconception Health Examination Project which covered all the 31 provinces in China's Mainland.Women intending to get pregnant within the next 6 months were enrolled between January 1,2010,and December 31,2012.Sociodemographic,gynecological and obstetric characteristics,and other relevant information were obtained through face-to-face interviews.Treponema pallidum particle agglutination assay test was used to detect positive samples of syphilis.Univariate and multivariate logistic regressions were performed to assess the associations between syphilis seropositivity and related factors.Results：The overall seroprevalence of syphilis （SPS） among the 2,044,126 women who received syphilis screening test during 2010-2012 was 0.37% （95% confidence interval [CI]：0.36-0.37%）.The SPS appeared 0.24% （95% CI：0.23-0.26%） and 0.66% （95% CI：0.59-0.72%） in women at 21-24 and 40-44 years of age,respectively,showing an increase of SPS,parallel with age,and the difference was significant.SPS was significantly higher in ethnic minorities than that in Han nationality （0.58% vs.0.35%,respectively,odds ratio [OR] =1.41,95% CI：1.30-1.53） and higher in workers than that in farmers （0.45% vs.0.36%,respectively,OR =1.27,95% CI：1.14-1.41）.Women with primary school or below level had a higher SPS as compared to those with college or above educational level （0.61% vs.0.32%,respectively,OR =2.49,95% CI：2.14-2.89）,and the increase reversely correlated with the levels of education.Women whose spouses were syphilis seropositive had significant greater risk （OR =48.26,95% CI：44.38-52.48） as compared those whose spouses were seronegative.Women who reported having had a history of sexually transmitted infections were more likely to be tested positive for serological syphilis （OR =27.17,95% CI：20.44-36.11） as compared to those without.Conclusions：High SPS is seen among reproductive-age women in rural China that calls for targeted interventions on syphilis prevention and control in this target population,with emphasis on those who are 35 years of age and above,less educated,being minor ethnicity,workers,and living in the western regions of China.
文摘目的分析329例孕妇血小板抗体阳性结果,探讨血小板抗体与妊娠次数和流产史的关系。方法选取2017年1月—2021年12月厦门大学附属第一医院329例住院孕妇为研究对象,采用固相凝集法进行血小板抗体筛查,分析孕妇血小板抗体阳性率与年龄、妊娠次数和流产史等因素之间的关系。结果329例孕妇中,血小板抗体阳性50例,阳性率15.198%;经产妇血小板抗体阳性率(19.431%)高于初产妇(7.627%),差异具有统计学意义(P<0.05),妊娠次数不同阳性率不同(1次7.627%,2次17.582%,>2次20.833%),三者差异具有统计学意义(P<0.05),组间趋势χ^(2)检验显示妊娠次数和血小板抗体有线性趋势(P<0.05);有流产史的孕妇抗体阳性率(23.529%)大于无流产史(13.027%),二者差异具有统计学意义(P<0.05);年龄对孕妇血小板抗体阳性率无影响(P>0.05)。结论孕妇妊娠次数和流产史与血小板阳性率密切相关,妊娠孕妇应该进行血小板抗体筛查,特别是有妊娠史和流产史的孕妇更应重视,这对于预防和减少孕期流产和胎儿/新生儿同种异体免疫血小板减少症(fetal and neonatal alloimmune thrombocytopenia,FNAIT)具有重要的临床意义。
