Preparation of lactic acid bacteria compound starter cultures based on pasting properties and its improvement of glutinous rice flour and dough

The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application.

Anticancer Activity of Rice Callus Suspension Cultures from Aromatic Varieties and Metabolites Regulated in Treated Cancer Cell Lines

Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCSC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCSC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCSC for 72 h, using propidium iodide staining and flow cytometry. RCSC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCSC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCSC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.

MACS-W:A modified optical clearing agent for imaging 3D cell cultures

Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.

Discussion on the Differences between Chinese and Western Cultures and Intercultural Translation

Due to different geographical backgrounds and development histories,there are certain cultural differences between China and Western countries,which often lead to cultural conflicts and seriously affect the efficiency and effectiveness of cultural exchanges.In the context of accelerating economic globalization and integration,as well as increasing cooperation among countries,in order to ensure the accuracy of translation,it is necessary to have a proficient and accurate understanding of the cultural differences between China and the West.By relying on these cultural differences,we can improve communication patterns and continuously enhance intercultural translation skills.

Biotransformation of Gastrodin by Cell Suspension Cultures of Catharanthus roseus

应用长春花 (Catharanthusroseus (L .)G .Don)悬浮细胞培养体系对天麻素进行了生物转化反应研究。经过8d培养形成一个转化产物 ,应用光谱方法鉴定转化产物的结构为对羟基苯甲醇 ,为天麻素水解后形成的甙元。

Effects of Fungal Elicitors on Cell Growth and Artemisinin Accumulation in Hairy Root Cultures of Artemisia annua

The artemisinin accumulation in the hairy root cultures of Artemisia annua L. was enhanced via a treatment of three fungal elicitors separately ( Verticillium dahliae Kleb., Rhizopus stolonifer (Ehrenb. ex Fr.) Vuill and Colletotrichum dematium (Pers.) Grove). Among these three elicitors, V. dahliae had the highest inducing efficiency, but none of them manifests any noticeable effects on the cell growth of the hairy root cultures. The artemisinin content of the hairy root cultures treated with V. dahliae elicitor was 1.12 mg/g DW, which was 45% higher than the control (0.77 mg/g DW). The results showed that elicitation was dependent on the elicitor concentration, the incubation period and the physiological stage at which the hairy root cultures were treated. In addition, the authors found that for V. dahliae , the optimum concentration was 0.4 mg carbohydrate per millilitre medium, the strongest response of A. annua hairy root cultures to the elicitation was at the late exponential growth stage, and the highest artemisinin content of the hairy root cultures was on the 4th day post treatment.

Productions of Taxol and Related Taxanes by Cell Suspension Cultures of Taxus yunnanensis

A high taxol yield cell line of Taxus yunnanensis Cheng et L. K. Fu keeps a high taxol_producing level after successive subcultures for more than eight years. In this study, eight taxanes were isolated from the suspension cell cultures of this cell line. Based on NMR and MS analyses, and comparison with literature data and standards, their structures were determined to be 2α,5α,10β_triacetoxy_14β_propionyloxy_4(20),11_taxadiene (1), 2α,5α,10β_triacetoxy_14β_(2′_methyl)_butyryloxy_4(20),11_taxadiene (2), 2α,5α,10β_14β_tetra_acetoxy_4 (20),11_taxadiene (3, taxuyunnanine C), 2α,5α,10β_triacetoxy_14β_(2′_methyl_3′_hydroxy)_butyryloxy_4(20),11_taxadiene (4, yunnanxane) and its 3′_epimer (5), baccatin Ⅳ (6), baccatin Ⅲ (7) and taxol (8), respectively. Among those compounds, 3, 5, 6 and 7 were reported to be isolated from the suspension cell cultures of T. yunnanensis for the first time. TLC and HPLC analyses indicated that the chemical constituents of the culture solution were similar to those of cultured cells. Moreover, the highest taxol content of this cell line reached 0.3% and the cell line could be applied for a large_scale culture.

Chemical Constituents of the Suspension Cell Cultures of Maytenus hookeri

Suspension cell cultures of Maytenus hookeri Loos. (Celastraceae) in SH media were established from the calli induced from the leaves and young steins of M. hookeri on MS media with the supplement of 2 mg/L 2,4-D and 0.1 mg/L KIN (kinetin). Ethyl acetate extract of the cultures showed inhibitory activities against Penicillium avellaneum UC-4376 which was sensitive to maytansinoids. Exhaustive isolation of natural products from a large scale of suspension cell cultures did not yield maytansine instead of affording nine compounds including one novel triterpenoid, named 2, 3-diacetoxyl maytenusone (1), and eight known ones including squalene (2), beta-sitosterol (3), 2', 3', 4-triacetyl-sitoindoside I (4), salaspermic acid (5), maytenonic acid (6), 2alpha-hydroxy-maytenonic acid (7), 6, 11,12-trihydroxy-8, 11, 13-abietrien-7-one (8) and 11, 12-dihydroxy-8, 11, 13-abietatrien-7-one (9) elucidated on the basis of 1D and 2D NMR data. The H-1-NMR and C-13-NMR assignments were made for 1, 5, 6 and 7, while the C-13-NMR assignments for 5 and 6 were revised. The chemical results suggested that the suspension cell cultures of M. hookeri did not produce maytansinoids under the reported experiment conditions.

Inheritance and Development of Cultures of Traditional Vernacular Dwellings in the Townscape Renovation Project of Guangxi,China

By analyzing background and present situation of townscape renovation project in Guangxi, endeavors for inheriting and developing cultures of traditional vernacular dwellings in the implementation of this project are discussed, and characteristics of traditional vernacular dwellings in the study area are summarized. Local vernacular dwellings can be classified in the following 3 ways on the basis of their own regional features:First, classifying by the origin and development history:stilt building, courtyard and non-courtyard building, western-style building; Second, by building materials:bamboo-wood building, stone building, adobe building, earth-stone building, brick-wood building, building of mixed materials; Third, by architectural form:stilt building, courtyard building, non-courtyard building. Stilt wooden building in northern Guangxi is a representative of traditional vernacular dwellings in the study area. By giving mature consideration to inheritance and development of cultures of traditional vernacular dwellings, this study proposes that proper renovation plans should be made according to actual conditions of the planning site; form renovation of vernacular dwellings should be taken as the new trend of renovation; traditional cultures should be further explored, demonstrated and protected. These suggestions are of great instructive significance for improving the cultural taste of townscapes.

The Collision and Coexistence between Chinese and American Cultures——The Collision and Coexistence between Two Generations in The Joy Luck Club

In many Chinese and American works,the differences of backgrounds,such as culture,customs and so on occupy a large part,through which readers can see the collision as well as coexistence between the two cultures.The Joy Luck Club has no exception.In this work,the communication under different cultural backgrounds between the generations generates a lot of collision,but they get coexistence in the end.In this paper,the author will analyze the continuous collision and the final coexistence of the four pairs of mothers and daughters,so that readers can learn more about the differences between Chinese and American cultures.

Expression Forms of Spiritual Cultures in Classical Chinese Gardens

By studying close links between classical Chinese gardens and traditional Chinese cultures,it was stressed that spiritual characteristics pursued by classical Chinese gardens were inevitable components of their expression forms,which were also closely related to classical Chinese philosophy,social system and other cultural phenomena.Characteristics of classical Chinese gardens in terms of psychology were analyzed,and it was proposed that they had particular cultural connotations in the spiritual expression and appeal,that is,natural view of "human-nature oneness",etiquette culture of "coordinating with the nature by etiquette",seclusion view of "living in seclusion and pursuing a peaceful life".Concrete expressions of spiritual cultures in classical Chinese gardens were discussed,specifically,the expression of natural views was demonstrated as "respecting the nature,all creatures having its own course(Tao,way of evolution),adopting measures suiting local conditions,skillfully borrowing from other landscapes,simulating natural landscapes";as for the expression of seclusion view,it was demonstrated as the expression of seclusion cultures by inscriptions and steles,the expression of "seclusion" by implicit garden settings;etiquette and hierarchy of classical Chinese gardens were discussed from the perspective of private garden layout and residence construction.On this basis,spiritual world of ancient Chinese were further stu-died,mental patterns of Chinese were believed as the source of classical gardens,and what those hills,water,plants,architecture as well as their combinations expressed were spiritual pursuits of Chinese people.

Exploring Regional Ecological Cultures in the Upper Reaches of the Yellow River in the Ming and Qing Dynasties from the Perspective of "Top Eight Views"

By studying typical "Top Eight Views" in cities like Xining,Lanzhou and Yinchuan in the upper reaches of the Yellow River,mountain and water landscapes in the study area were analyzed in terms of natural ecology;bridges,canals,ferries,pavilions,mansions,towers,temples,historical sites,production and living landscapes were analyzed in terms of humanistic ecology.On this basis,"Top Eight Views" in the upper reaches of the Yellow River were compared and the following conclusions obtained:mountain landscapes were distributed in the whole region,waterscapes unevenly valued in different cities,bridges,canals and ferries mostly located in Ningxia,temples commonly found in all cities,garden arts introduced into cities,traditional cultures carried forward by historical relics.Then the relationship between "Top Eight Views" and regional eco-cultures was analyzed,and it was proposed that humanistic and cultural connotations of these "Top Eight Views" contributed a lot to beautifying urban environment,satisfying needs of the locals' spiritual life,carrying forward local history and culture,enriching urban cultural connotations,expanding living spaces of local residents and improving integrated functions of cities."Top Eight Views" culture complied with the construction gist of urban ecological cultures,and was of great referential value for the construction of urban ecological cultures in the upper reaches of the Yellow River,and also the healthy,scientific and sustainable development of local cities.

Detection of Mycoplasma Contamination in Animal Cell Cultures

[ Objective ] To develop a rapid efficient method for detecting mycoplasma contamination in cell cultures. [ Method] A pair of primers was designed according to two highly conserved nucleotide sequences of the 16S RNA from six kinds of mycoplasma that commonly contaminated cells. Then the mycoplasma contamination of 25 cell samples was defected by PCR and DNA fluorescence staining. EResultl When these cell samples were detected by DNA fluorescence staining, the positive rate and probable positive rate were respectively 24% and 16%. And when they were detected by PCR, the positive rate was 36%. [ Condusion] The PCR method is more sensitive and specific than the DNA fluorescence staining, and combining these two methods is the optimal way to detect mycoplasma contamination in cell cultures.

Research on the Application of Immortal Cultures in Tourism Planning——A Case Study of Tourism Development of Xianshi Town in Zigong City of China

Through analyzing the relationship between immortal cultures and tourist activities, the authors proposed that birth of immortal thought was closely related to early tourist activities. The core idea of immortal cultures was in conformity with modern leisure cultures. Tourist resources in Ancient Xianshi Township were re-explored, and application of immortal cultures in tourism development of the town was studied. The authors proposed that both "immortal" and "salt" should be valued in the tourism development of ancient Xianshi Township, interaction and integration of immortal stories, immortal traces and scenic areas should be stressed in the application of immortal cultures, so as to incorporate "immortal bath" and modern salt bath, and to combine creation of fairyland with modern sightseeing and leisure agriculture.

A Comparison between Chinese and Western Cultures

Cultural awareness, which plays a big part in our efforts of culture development, comes from active comparison and contrast of different cultures, especially between the domestic and foreign cultures. Though the Chinese culture and the western cultures have much in common, they differ in value and morality concepts, Social customs, etiquette, relations, the spirit of individuality and so on. We should bear in mind this cultural awareness when making cultural policies, or having cross-cultural communications, which enables us to become more active in culture development.

Influence of Confucian and Taoist Cultures on Vernacular Dwellings in Jiaodong Area of China

By analyzing influence of Confucian and Taoist cultures on vernacular dwellings in Jiaodong area,especially the influence of "the doctrine of the mean" on architectural layouts of local dwellings,influence of benevolence and social institutions on the spatial sequence of local dwellings,it was pointed out that Confucian cultures enhanced the hierarchy of spaces,boundaries between internal and external spaces,symmetric layouts and orderly rhythm of courtyards;and meanwhile,the influence of Taoist cultures on the formation of villages in Jiaodong area was also analyzed,the natural philosophy implied in local dwellings was highlighted,such as "Dealing with the nature with natural laws","Going in a natural way","Governing by doing nothing that goes against the nature" and "Respecting natural properties".Based on field investigation of existing vernacular dwellings,common and plain cultural value,realistic significance and sustainability of these dwellings were explored to provide theoretical and practical foundation for the design of new folk houses.

Associative Meanings of Colors in Chinese and English Cultures

Colors are endowed into many different associative meanings by different peoples and countries under the various cultural backgrounds and people make it express testimonial and depreciated meaning. There is not only the same aspect, but also the differences. Moreover, some factors such as geographical environment, public sentiment custom, thinking mode, religious belief, and national psychology also influence it. Each kind of color is regarded the difference for every nation's person. This article analyzes and investigates the associative meaning of the color in the Chinese and English cultures. And then it will let the reader learn well and more the associative meaning under the different cultural background.

Differences Between British and Americans’ Cultures in Values

Values are the most important issue in identifying one particular culture.Social values are the feelings people have about what is important,worthwhile,and just.In this paper,the differences between British and American values are discussed in two aspects which mainly lie respectively in the comparisons of values and characteristics in both cultures.

Perspectives on the Values of Rose in Eastern and Western Languages and Cultures

The cultural differences between the east and the west are reflected in their own languages. Vocabulary is the basic building material of a language. It is the basic linguistic unit of cultural connotation and message. Through the analysis of the word ＂rose＂, this paper attempts to find the different connotations in Chinese and western cultures. It also tentatively explores the significance of introducing cultural background in foreign language teaching.

Misunderstanding Due to Different Cultures

There are fences between different cultures. People from different cultural backgrounds may sometimes misunderstand each oth er. In this passage, we merely take Chinese culture background and American culture background as examples.