Influence of ear tags on the results of body composition analysis in mice

Background:The aim of this study was to investigate the influence of marking meth-ods on the outcomes of body composition analysis and provide guidance for the se-lection of marking methods in mouse body composition analysis.Methods:Male C57BL/6J mice aged 6 weeks were randomly assigned for pre-and post-ear tagging measurements.The body composition of the mice was measured using a small animal body composition analyzer,which provided measurements of the mass of fat,lean,and free fluid.Then,the mass of fat,lean and free fluid to body weight ratio was gained.Further data analysis was conducted to obtain the range and coeffi-cient of variation in body composition measurements for each mouse.The distribution of fat and lean tissue in the mice was also analyzed by comparing the fat-to-lean ratio.Results:(1)The mass of all body composition components in the ear tagging group was significantly lower than that in the control group.(2)There was a significant in-crease in the range and coefficient of variation of body composition measurements between the ear tagging group and the control group.(3)The fat-to-lean ratio in the ear tagging group was significantly lower than that in the control group.Conclusions:Ear tagging significantly lowered the results of body composition analy-sis in mice and higher the results of measurement error.Therefore,ear tagging should be avoided as much as possible when conducting body composition analysis experi-ments in mice.

Evolution of microstructure and mechanical properties in multi-layer 316L-TiC composite fabricated by selective laser melting additive manufacturing

In this study,the microstructure and mechanical properties of a multi-layered 316L-TiC composite material produced by selective laser melting(SLM)additive manufacturing process are investigated.Three different layers,consisting of 316L stainless steel,316L-5 wt%TiC and 316L-10 wt%TiC,were additively manufactured.The microstructure of these layers was characterized by optical microscopy(OM)and scanning electron microscopy(SEM).X-ray diffraction(XRD)was used for phase analysis,and the mechanical properties were evaluated by tensile and nanoindentation tests.The microstructural observations show epitaxial grain growth within the composite layers,with the elongated grains growing predominantly in the build direction.XRD analysis confirms the successful incorporation of the TiC particles into the 316L matrix,with no unwanted phases present.Nanoindentation results indicate a significant increase in the hardness and modulus of elasticity of the composite layers compared to pure 316L stainless steel,suggesting improved mechanical properties.Tensile tests show remarkable strength values for the 316L-TiC composite samples,which can be attributed to the embedded TiC particles.These results highlight the potential of SLM in the production of multi-layer metal-ceramic composites for applications that require high strength and ductility of metallic components in addition to the exceptional hardness of the ceramic particles.

Experimental Study on Corrosion of Stainless Steel in Low Temperature Multi effect Seawater Desalination

Starting from the corrosion mechanism,this paper analyzes the characteristics of various types of stainless steel and selects the best performance composite plate composite plate stainless steel.Analyze and select the most suitable corrosion detection method based on specific practical multi working conditions,discuss the interference factors that affect metal corrosion during experimental simulation,and the advantages of newly developed sheet metal.The new development of composite board panels,with the substrate and composite materials applying their respective capabilities for MED,will bring breakthrough progress to the scientific research and engineering applica-tion of composite boards.

Tensile and wear properties of TiC reinforced 420 stainless steel fabricated by in situ synthesis

TiC particle reinforced 420 stainless steel matrix composites were fabricated, and the microstructure, tensile properties and wear resistance of the composites were studied. The experimental results indicate that the distribution of TiC particles with size of 5 to 10 μm in diameter is uniform if the volume fraction of TiC is lower than 6%. However, slight agglomeration can be observed when the TiC content exceeds 6%. With the increase of TiC content the tensile and yield strength of the composites prepared increases and reaches the maximum when the volume fraction of TiC increases to 5%. Further increase of TiC content causes reductions of yield and tensile strength. The ductility of the composites shows a monotone decrease with the increase of TiC addition. The introduction of TiC into 420 stainless steel results in significant improvement on wear resistance, which reaches a steady level when the volume fraction of TiC increases to 11% and does not show obvious variation if the TiC content is further increased.

Effect of Annealing on Fly-Line Microstructure and Properties of Explosively Composited Stainless Steel-Stainless Steel Plates

Effect of annealing on "fly-line"(adiabatic sheer line) microstructure and properties of explosively composited stainless steel-stainless steel plates was studied.Results show that the flyline microstructure will diminish through certain annealing process,while the cracks formed from fly-line microstructure will remain.Therefore,fly-line microstructure can be considered as a plastic deformation microstructure and crack source s meanwhile its formation is considered as a special plastic deformation mechanism of metal under explosive load.

Effect of heat treatment on the microstructure and mechanical properties of structural steel–mild steel composite plates fabricated by explosion welding

Two dissimilar steel plates,structural steel and mild steel,were joined by explosion welding to form a composite.The composite was then heat-treated by quenching at 840℃ for 30 min followed by tempering at 200℃ for 3 h.The microstructure was investigated under an optical microscope and a scanning electron microscope.The mechanical properties were measured using Vickers microhardness and Charpy impact tests.The results show a deformed interface with typical wave features at the welding zone,but no defects were observed.Moreover,the ferrite in the parent plate in the weld zone was elongated due to the strong plastic deformation from the explosion.After heat treatment,the hardness of the flyer plate(structural steel)was over HV0.2800,while that of the parent plate(mild steel)was HV0.2200.The increase in hardness was due to the presence of martensite.Moreover,the average impact energy was increased from 18.5 to 44.0 J following heat treatment;this is because of the formation of recrystallized grains at the weld interface,which is due to the dynamic recovery and local recrystallization,and the strong elemental diffusion that occurred between the two plates.

Experimental study and analysis on fatigue stiffness of RC beams strengthened with CFRP and steel plate

The objective of this work is to investigate the fatigue behavior of reinforced concrete(RC) beams strengthened with externally bonded carbon fiber reinforced polymer(CFRP) and steel plate. An experimental investigation and theoretical analysis were made on the law of deflection development and stiffness degradation, as well as the influence of fatigue load ranges. Test results indicate that the law of three-stage change under fatigue loading is followed by both midspan deflection and permanent deflection, which also have positive correlation with fatigue load amplitude. Fatigue stiffness of composite strengthened beams degrades gradually with the increasing of number of cycles. Based on the experimental results, a theoretical model by effective moment of inertia method is developed for calculating the sectional stiffness of such composite strengthened beams under fatigue loading, and the calculated results are in good agreement with the experimental results.

Beneficial use of stainless steel EAF slag as composite cement admixture and its heavy metals leaching risk analysis

This study analyzes the feasibility of using stainless steel electric arc furnace （EAF） slag as composite cement admixture and the risk of leaching-out of heavy metals. The results show that the stainless steel EAF slag, mainly made up of Ca2 SiO4, Ca3 Mg （ SiO4 ） 2, some Cr-containing minerals and in small particle size, is easily ground and has cementitious activity. EAF slag, when used as cement admixture, can be added with a maximum percentage of 32%. It can meet the strength requirements of the standard P · C 32.5 cement. When the mixing percentage is decreased to 25 % , the strength of the cement can be increased to that of P · C 42.5 cement. Other main quality indexes of composite cement, such as the setting time and stability, also satisfy standard requirements. The results also show that most of the heavy metals in the stainless steel EAF slag exist in a stable speciation. The concentration of heavy metals that leach out from the stainless steel EAF slag and the composite cement products is far lower than the standard limit of hazardous wastes. The main heavy metal, chromium, exists as less hazardous trivalent chromium. Therefore, the risk of heavy metals leaching out from the stainless steel EAF slag is low. The internal exposure index （IRa） and the external exposure index （Iγ） of the stainless steel EAF slag are both lower than 1.0, satisfying the standard requirements of the state for the radionuclides of building materials. Therefore, stainless steel EAF slag can be safely used as admixture to produce composite cement.

Structure and sliding wear behavior of 321 stainless steel/Al composite coating deposited by high velocity arc spraying technique

A typical 321 stainless steel/aluminum composite coating (321/Al coating) was prepared by high velocity arc spraying technique (HVAS) with 321 stainless steel wire as the anode and aluminum wire as the cathode. The traditional 321 stainless steel coating was also prepared for comparison. Tribological properties of the coatings were evaluated with the ring-block wear tester under different conditions. The structure and worn surface of the coatings were analyzed by scanning electron microscopy(SEM), X-ray diffractometry(XRD) and energy dispersion spectroscopy(EDS). The results show that, except for aluminum phase addition in the 321/Al coating, no other phases are created compared with the 321 coating. However, due to the addition of aluminum, the 321/Al coating forms a type of "ductile/hard phases inter-deposited" structure and performs quite different tribological behavior. Under the dry sliding condition, the anti-wear property of 321/Al coating is about 42% lower than that of 321 coating. But under the oil lubricated conditions with or without 32 h oil-dipping pretreatment, the anti-wear property of 321/Al coating is about 9% and 5% higher than that of 321 coating, respectively. The anti-wear mechanism of the composite coating is mainly relevant to the decrease of oxide impurities and the strengthening action resulted from the "ductile/hard phases inter-deposited" coating structure.

Analysis and seismic tests of composite shear walls with CFST columns and steel plate deep beams

A composite shear wall concept based on concrete filled steel tube （CFST） columns and steel plate （SP） deep beams is proposed and examined in this study. The new wall is composed of three different energy dissipation elements： CFST columns; SP deep beams; and reinforced concrete （RC） strips. The RC strips are intended to allow the core structural elements - the CFST columns and SP deep beams - to work as a single structure to consume energy. Six specimens of different configurations were tested under cyclic loading. The resulting data are analyzed herein. In addition, numerical simulations of the stress and damage processes for each specimen were carried out, and simulations were completed for a range of location and span-height ratio variations for the SP beams. The simulations show good agreement with the test results. The core structure exhibits a ductile yielding mechanism characteristic of strong column-weak beam structures, hysteretic curves are plump and the composite shear wall exhibits several seismic defense lines. The deformation of the shear wall specimens with encased CFST column and SP deep beam design appears to be closer to that of entire shear walls. Establishing optimal design parameters for the configuration of SP deep beams is pivotal to the best seismic behavior of the wall. The new composite shear wall is therefore suitable for use in the seismic design of building structures.

Influence of chemical composition and cold deformation on aging precipitation behavior of high nitrogen austenitic stainless steels

The influence of chemical composition and cold deformation on aging precipitation behavior of 18Cr-16Mn-2Mo-I.IN （HNS-A）, 18Cr-16Mn-I.3N （HNS-B）, 18Cr-18Mn-2Mo-0.96N （HNS-C） and 18Cr-18Mn-2Mo-0.77N （I-INS-D） high nitrogen austenitic stainless steels was investigated. The results show that the ＂nose＂ temperatures and incubation periods of the initial time-temperature-precipitation （TTP） curves of aged HNSs are found to be 850 ℃, 60 s; 850 ℃, 45 s; 850 ℃, 60 s and 900 ℃, 90 s, respectively. Based on the analysis of SAD patterns, the coarse cellular Cr2N precipitate which presents a lamellar structure has a hexagonal structure of a=0.478 nm and c=0.444 nm. The Z phase corresponding to a composition of Fe36Cr^2Mo10, is determined to be a body-centered cubic structure ofa=0.892 nm. The precipitating sensitivity presents no more difference with the nitrogen content increasing from 0.77% to 0.96%, but exhibits so obviously that the cellular precipitates nearly overspread the whole field. The addition of Mo element can restrain the TTP curves moving left and down, which means decreasing the sensitivity of aging precipitation. With increasing the cold deformation, the sensitivity of precipitation increases obviously.

Lateral load-carrying capacity analyses of composite shear walls with double steel plates and filled concrete with binding bars

A method is developed to predict the lateral load-carrying capacity of composite shear walls with double steel plates and filled concrete with binding bars(SCBs). Nonlinear finite element models of SCBs were established by using the finite element tool, Abaqus. Tie constraints were used to connect the binding bars and the steel plates. Surface-to-surface contact provided by the Abaqus was used to simulate the interaction between the steel plate and the core concrete. The established models could predict the lateral load-carrying capacity of SCBs with a reasonable degree of accuracy. A calculation method was developed by superposition principle to predict the lateral load-carrying capacity of SCBs for the engineering application. The concrete confined by steel plates and binding bars is under multi-axial compression; therefore, its shear strength was calculated by using the Guo-Wang concrete failure criterion. The shear strength of the steel plates of SCBs was calculated by using the von Mises yielding criterion without considering buckling. Results of the developed method are in good agreement with the testing and finite element results.

Investigation of Sintering Technology for Composite of Stainless Steel and Partially Stabilized Zirconia

The sintering technology for mixed powdered extrusion rods of different proportions of stainless steel to magnesia partially stabilized zirconia (PSZ) was investigated. The effects of some sintering parameters including holding time, atmosphere and protective gas pressure on shrinkage, relative density, microstructure, micro-Vickers hardness and compression strength of sintered samples were mainly researched. The experimental results are as follows: (1) The shrinkage and the relative density of the sintered samples decrease as increasing stainless steel content in the composite, except for the case containing 90 percent of stainless steel; (2) The porosity in PSZ matrix rises as increasing the stainless steel content in the composite; (3) Longer sintering holding time, higher sintering vacuum and gas-pressure sintering process not only enhance the relative density, but also improve microstructure of composite; (4) Micro-Vickers hardness of PSZ matrix decreases as increasing stainless steel content, while that of stainless steel particles in sintered samples varies unnoticeably.

CYCLIC OXIDATION OF Ni-La_2O_3 COMPOSITE COATINGS ELECTRODEPOSITED ON NICKEL AND Fe26Cr STAINLESS STEEL

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Oxidation Behavior of Fe26Cr1Mo Stainless Steel in the Presence of Ni-La_2O_3 Electrodeposited Composite Film

The oxidation behaviors of Fe26Cr1Mo with and without the Ni La 2O 3 electrodeposited composite film have been investigated by thermogravimetric analysis (TGA) and a scanning electron microscope equipped with an energy dispersive analytical X ray system(SEM/EDAX). The experimental results show that the oxide scale growing on Fe26Cr1Mo exposed at 900 ℃ spalled severely during cooling, while after the stainless steel was coated with the Ni La 2O 3 electrodeposited composite film, its high temperature cyclic oxidation resistance was significantly improved. The reason is that a La 2O 3 modified NiO scale, which has a superior adhesion to the substrate, was formed on the Fe26Cr1Mo stainless steel coated with Ni La 2O 3 composite film.

Microstructure and properties of composite of stainless steel and partially stabilized zirconia

To fabricate the metal-ceramics multi-layer hollow functionally gradient materials(FGMs) that might meet the requirement of repeated service and long working time of high temperature burners, such as spacecraft engine, the microstructure and properties of composite of stainless steel and partially stabilized zirconia were investigated. Samples of different proportions of stainless steel to partially yttria-stabilized zirconia were fabricated by powder extrusion and sintering method. Shrinkage, relative density, microstructure, micro-Vickers hardness, compression strength, bending strength, fractography morphology and electrical resistivity of sintered samples with different proportions of stainless steel were measured. The results show that threshold of metallic matrix composite(MMC) is approximately equal to 60%(volume fraction) stainless steel. The samples with 0 to 50%(volume fraction) stainless steel indicate ceramic brittleness and non-cutability, and the samples with 70% to 100%(volume fraction) stainless steel indicate metallic plasticity and cutability.

Influence of Non-smooth Surface on Tribological Properties of Glass Fiber-epoxy Resin Composite Sliding against Stainless Steel under Natural Seawater Lubrication

With the development of bionics, the bionic non-smooth surfaces are introduced to the field of tribology. Although non-smooth surface has been studied widely, the studies of non-smooth surface under the natural seawater lubrication are still very fewer, especially experimental research. The influences of smooth and non-smooth surface on the frictional properties of the glass fiber-epoxy resin composite（GF/EPR） coupled with stainless steel 316 L are investigated under natural seawater lubrication in this paper. The tested non-smooth surfaces include the surfaces with semi-spherical pits, the conical pits, the cone-cylinder combined pits, the cylindrical pits and through holes. The friction and wear tests are performed using a ring-on-disc test rig under 60 N load and 1000 r/min rotational speed. The tests results show that GF/EPR with bionic non-smooth surface has quite lower friction coefficient and better wear resistance than GF/EPR with smooth surface without pits. The average friction coefficient of GF/EPR with semi-spherical pits is 0.088, which shows the largest reduction is approximately 63.18% of GF/EPR with smooth surface. In addition, the wear debris on the worn surfaces of GF/EPR are observed by a confocal scanning laser microscope. It is shown that the primary wear mechanism is the abrasive wear. The research results provide some design parameters for non-smooth surface, and the experiment results can serve as a beneficial supplement to non-smooth surface study.

TiC-Maraging stainless steel composite:microstructure, mechanical and wear properties

Particulate TiC reinforced 17-4PH and 465 maraging stainless steel matrix composites were processed by conventional powder metallurgy （P/M）. TiC-maraging stainless steel composites with theoretical density 〉97% were produced using conventional P/M. The microstructure, and mechanical and wear properties of the composites were evaluated. The microstructure of the composites consisted of （core-rim structure） spherical and semi-spherical TiC particles depending on the wettability of the matrix with TiC particles. In TiC-maraging stainless steel composites, 465 stainless steel binder phase showed good wettability with TiC particles. Some microcracks appeared in the composites, indicating the presence of tensile stresses in the composites produced during sintering. The typical properties, hardness, and bend strength were reported for the composites. After heat treatment and aging, an increase in hardness was observed. The increase in hardness was at- tributed to the aging reaction in maraging stainless steel. The specific wear behavior of the composites strongly depends on the content of TiC particles and their interparticle spacing, and on the heat treatment of the maraging stainless steel.

Experimental and analytical study on seismic behavior of steel-concrete multienergy dissipation composite shear walls

In this paper, a steel-concrete multi-energy dissipation composite shear wall, comprised of steel-reinforced concrete （SRC） columns, steel plate （SP） deep beams, a concrete wall and energy dissipation strips, is proposed. In order to study the multi-energy dissipation behavior and restorability after an earthquake, two stages of low cyclic loading tests were carded out on ten test specimens. In the first stage, test on five specimens with different number of SP deep beams was carried out, and the test lasted until the displacement drift reached 2%. In the second stage, thin SPs were welded to both sides of the five specimens tested in the first stage, and the same test was carried out on the repaired specimens （designated as new specimens）. The load-bearing capacity, stiffness, ductility, hysteretic behavior and failure characteristics were analyzed for both stages and the results are discussed herein. Extrapolating from these results, strength calculation models and formulas are proposed herein and simulations using ABAQUS carried out, they show good agreement with the test results. The study demonstrates that SRC columns, SP deep beams, concrete wall and energy dissipation strips cooperate well and play an important role in energy dissipation. In addition, this study shows that the shear wall has good recoverability after an earthquake, and that the welding of thin SP＇s to repair a deformed wall is a practicable technique.

INFLUENCE OF PHASE COMPOSITION ON PITTING CORROSION RESISTANCE OF SPUTTERED COATING OF 1Cr18Ni9Ti STAINLESS STEEL

A series of single bcc,bcc plus fcc duplex and single fcc microcrystalline coatings of 1Cr18Ni9Ti stainless steel were prepared by using sputtering technique.The resistance against pitting corrosion was studied by measurements of pitting corrosion potentials and electrochemical noise during initiation of corrosion pits.The results show that the sputtered coatings with single bcc phase or single fcc structure are more resistant to pitting corrosion than those with bcc plus fcc duplex phase structure.