Administration of platelet rich plasma(PRP) and bone marrow aspirate concentrate(BMAC) has shown some promise in the treatment of neurological conditions;however, there is limited information on combined administratio...Administration of platelet rich plasma(PRP) and bone marrow aspirate concentrate(BMAC) has shown some promise in the treatment of neurological conditions;however, there is limited information on combined administration. As such, the purpose of this study was to assess safety and functional outcomes for patients administered combined autologous PRP and BMAC for spinal cord injury(SCI). This retrospective case series included seven patients who received combined treatment of autologous PRP and BMAC via intravenous and intrathecal administration as salvage therapy for SCI. Patients were reviewed for adverse reactions and clinical outcomes using the Oswestry Disability Index(ODI) for up to 1 year, as permitted by availability of follow-up data. Injury levels ranged from C3 through T11, and elapsed time between injury and salvage therapy ranged from 2.4 months to 6.2 years. Post-procedure complications were mild and rare, consisting only of self-limited headache and subjective memory impairment in one patient. Four patients experienced severe disability prior to PRP combined with BMAC injection, as evidenced by high(> 48/100) Oswestry Disability Index scores. Longitudinal Oswestry Disability Index scores for two patients with incomplete SCI at C6 and C7, both of whom had cervical spine injuries, demonstrated a decrease of 28–40% following salvage therapy, representing an improvement from severe to minimal disability. In conclusion, intrathecal/intravenous co-administration of PRP and BMAC resulted in no significant complications and may have had some clinical benefits. Larger clinical studies are needed to further test this method of treatment for patients with SCI who otherwise have limited meaningful treatment options. This study was reviewed and approved by the Ohio Health Institutional Review Board(IRB No. 1204946) on May 16, 2018.展开更多
The process of treating the PET tire cord (210D/3×2) by low temperature nitrogen and airlow temperature plasma and dipping in RFL (resorcinol-formaldehyde-latex) adhesive system wasstudied.The H-pull test value c...The process of treating the PET tire cord (210D/3×2) by low temperature nitrogen and airlow temperature plasma and dipping in RFL (resorcinol-formaldehyde-latex) adhesive system wasstudied.The H-pull test value can be improved from about 35 N/cm of untreated sample to 55N/cm of nitrogen plasma treated sample.The photograph of SEM demonstrates that the adhe-sion between nitrogen plasma treated tire cord and rubber has reached the strength of rubber.Airplasma treatment has bad effect on adhesion improvement.The mechanism of plasma treatment on the surface of the PET tire cord and the mechanism ofadhesion improvement are investigated by diffuse reflection infra-red spectrum and XPS (X-rayphotoelectron spectroscopy).From the spectral analysis,it is believed that the contribution to im-provement of the adhesion of the tire cord is the oxidation on the surface.The serious breaking ofthe chemical bonds on surface can cause the adhesion inferior.展开更多
Diabetic patients often exhibit delayed or incomplete progress in the healing of acute wounds,owing to poor blood perfusion.Platelet-rich plasma(PRP)has attracted much attention as a means to improve wound healing,bec...Diabetic patients often exhibit delayed or incomplete progress in the healing of acute wounds,owing to poor blood perfusion.Platelet-rich plasma(PRP)has attracted much attention as a means to improve wound healing,because it contains high growth factor concentrations.However,the burst-like release of PRP growth factors results in a short halflife of these therapeutic proteins,thus greatly limiting the therapeutic effect.In this study,we prepared PRP from human umbilical cord blood and developed an in situ photocrosslinkable PRP hydrogel glue(HNPRP)by adding a photoresponsive hyaluronic acid(HA-NB)into PRP.The HNPRP hydrogel allowed for controlled release of plateletderived growth factor-BB(PDGF-BB)and transforming growth factor-β(TGF-β)for up to 28 days.In vitro cell culture showed that HNPRP promoted migration of fibroblasts and keratinocytes as well as PRP and did not reveal the advantages of HNPRP.However,in a diabetic rat skin wound model,HNPRP treatment promoted faster wound closure.Furthermore,the HNPRP group,compared with the control,PRP and hydrogel only groups,showed significantly greater re-epithelialization and numbers of both newly formed and mature blood vessels.The HNPRP group also displayed higher collagen formation than did the control group.In conclusion,HNPRP enhances angiogenesis and skin regeneration and consequently achieves faster wound healing,thus extending its potential for clinical applications to treat diabetic skin wounds.展开更多
背景:研究报道富血小板血浆可以激活和加速压力性损伤的愈合。目的:探讨富血小板血浆治疗脊髓损伤患者压力性损伤的效果。方法:选择2017年7月至2019年12月四川大学华西医院康复医学中心收治的脊髓损伤并发3期、4期压力性损伤患者40例,...背景:研究报道富血小板血浆可以激活和加速压力性损伤的愈合。目的:探讨富血小板血浆治疗脊髓损伤患者压力性损伤的效果。方法:选择2017年7月至2019年12月四川大学华西医院康复医学中心收治的脊髓损伤并发3期、4期压力性损伤患者40例,按照随机数字表法分2组,每组20例:对照组进行常规伤口护理及标准化的康复干预,观察组在常规伤口护理及标准化康复治疗的基础上进行自体源性富血小板血浆干预,每周1次。干预前及干预后2,4,6,8周,采用压疮愈合量表(pressure ulcer scale for healing,PUSH)动态评估伤口面积、伤口类型、渗液量,比较两组总分、各条目得分及临床综合疗效。研究获得四川大学华西医院生物医学伦理委员会批准[批准号:2019年审(622)号]。结果与结论:①观察组干预后各时间点的PUSH评分总分及伤口面积、伤口类型、渗液量评分低于干预前(P<0.01),对照组干预后6,8周的PUSH评分总分及伤口面积、伤口类型、渗液量评分低于干预前(P<0.01);②观察组干预后4,6,8周的PUSH评分总分与渗液量评分低于对照组,干预后6,8周的伤口类型评分低于对照组(P<0.01);③观察组治疗后的显效率高于对照组(100%,35%,P<0.001);④结果表明,富血小板血浆治疗脊髓损伤压力性损伤具有明显的疗效。展开更多
文摘Administration of platelet rich plasma(PRP) and bone marrow aspirate concentrate(BMAC) has shown some promise in the treatment of neurological conditions;however, there is limited information on combined administration. As such, the purpose of this study was to assess safety and functional outcomes for patients administered combined autologous PRP and BMAC for spinal cord injury(SCI). This retrospective case series included seven patients who received combined treatment of autologous PRP and BMAC via intravenous and intrathecal administration as salvage therapy for SCI. Patients were reviewed for adverse reactions and clinical outcomes using the Oswestry Disability Index(ODI) for up to 1 year, as permitted by availability of follow-up data. Injury levels ranged from C3 through T11, and elapsed time between injury and salvage therapy ranged from 2.4 months to 6.2 years. Post-procedure complications were mild and rare, consisting only of self-limited headache and subjective memory impairment in one patient. Four patients experienced severe disability prior to PRP combined with BMAC injection, as evidenced by high(> 48/100) Oswestry Disability Index scores. Longitudinal Oswestry Disability Index scores for two patients with incomplete SCI at C6 and C7, both of whom had cervical spine injuries, demonstrated a decrease of 28–40% following salvage therapy, representing an improvement from severe to minimal disability. In conclusion, intrathecal/intravenous co-administration of PRP and BMAC resulted in no significant complications and may have had some clinical benefits. Larger clinical studies are needed to further test this method of treatment for patients with SCI who otherwise have limited meaningful treatment options. This study was reviewed and approved by the Ohio Health Institutional Review Board(IRB No. 1204946) on May 16, 2018.
文摘The process of treating the PET tire cord (210D/3×2) by low temperature nitrogen and airlow temperature plasma and dipping in RFL (resorcinol-formaldehyde-latex) adhesive system wasstudied.The H-pull test value can be improved from about 35 N/cm of untreated sample to 55N/cm of nitrogen plasma treated sample.The photograph of SEM demonstrates that the adhe-sion between nitrogen plasma treated tire cord and rubber has reached the strength of rubber.Airplasma treatment has bad effect on adhesion improvement.The mechanism of plasma treatment on the surface of the PET tire cord and the mechanism ofadhesion improvement are investigated by diffuse reflection infra-red spectrum and XPS (X-rayphotoelectron spectroscopy).From the spectral analysis,it is believed that the contribution to im-provement of the adhesion of the tire cord is the oxidation on the surface.The serious breaking ofthe chemical bonds on surface can cause the adhesion inferior.
文摘Diabetic patients often exhibit delayed or incomplete progress in the healing of acute wounds,owing to poor blood perfusion.Platelet-rich plasma(PRP)has attracted much attention as a means to improve wound healing,because it contains high growth factor concentrations.However,the burst-like release of PRP growth factors results in a short halflife of these therapeutic proteins,thus greatly limiting the therapeutic effect.In this study,we prepared PRP from human umbilical cord blood and developed an in situ photocrosslinkable PRP hydrogel glue(HNPRP)by adding a photoresponsive hyaluronic acid(HA-NB)into PRP.The HNPRP hydrogel allowed for controlled release of plateletderived growth factor-BB(PDGF-BB)and transforming growth factor-β(TGF-β)for up to 28 days.In vitro cell culture showed that HNPRP promoted migration of fibroblasts and keratinocytes as well as PRP and did not reveal the advantages of HNPRP.However,in a diabetic rat skin wound model,HNPRP treatment promoted faster wound closure.Furthermore,the HNPRP group,compared with the control,PRP and hydrogel only groups,showed significantly greater re-epithelialization and numbers of both newly formed and mature blood vessels.The HNPRP group also displayed higher collagen formation than did the control group.In conclusion,HNPRP enhances angiogenesis and skin regeneration and consequently achieves faster wound healing,thus extending its potential for clinical applications to treat diabetic skin wounds.
文摘背景:研究报道富血小板血浆可以激活和加速压力性损伤的愈合。目的:探讨富血小板血浆治疗脊髓损伤患者压力性损伤的效果。方法:选择2017年7月至2019年12月四川大学华西医院康复医学中心收治的脊髓损伤并发3期、4期压力性损伤患者40例,按照随机数字表法分2组,每组20例:对照组进行常规伤口护理及标准化的康复干预,观察组在常规伤口护理及标准化康复治疗的基础上进行自体源性富血小板血浆干预,每周1次。干预前及干预后2,4,6,8周,采用压疮愈合量表(pressure ulcer scale for healing,PUSH)动态评估伤口面积、伤口类型、渗液量,比较两组总分、各条目得分及临床综合疗效。研究获得四川大学华西医院生物医学伦理委员会批准[批准号:2019年审(622)号]。结果与结论:①观察组干预后各时间点的PUSH评分总分及伤口面积、伤口类型、渗液量评分低于干预前(P<0.01),对照组干预后6,8周的PUSH评分总分及伤口面积、伤口类型、渗液量评分低于干预前(P<0.01);②观察组干预后4,6,8周的PUSH评分总分与渗液量评分低于对照组,干预后6,8周的伤口类型评分低于对照组(P<0.01);③观察组治疗后的显效率高于对照组(100%,35%,P<0.001);④结果表明,富血小板血浆治疗脊髓损伤压力性损伤具有明显的疗效。
