A new neophysalin, named 5α-hydroxy-25,27-dihydro-4,7-didehydro-7-deoxyneophysalin A（1）, along with three other known neophysalins （2-4） were isolated from the calyxes of Physalis alkekengi L. var.francheti （Ma...A new neophysalin, named 5α-hydroxy-25,27-dihydro-4,7-didehydro-7-deoxyneophysalin A（1）, along with three other known neophysalins （2-4） were isolated from the calyxes of Physalis alkekengi L. var.francheti （Mast.） Makino. The structure of 1 was determined by means of 1D and 2D NMR, UV, IR and mass spectra. Compound 1 displayed potent cytotoxicities in vitro against PC- 3 and LNCaP cell lines.展开更多
Objective: As a traditional medicinal plant listed in the Chinese Pharmacopeia, Physalis alkekengi L. var. franchetii(Mast.) Makino(PAF) has a long medicinal history and high economic value. PAF has immunomodulatory p...Objective: As a traditional medicinal plant listed in the Chinese Pharmacopeia, Physalis alkekengi L. var. franchetii(Mast.) Makino(PAF) has a long medicinal history and high economic value. PAF has immunomodulatory properties and can be used to treat acute lung injury and eczema.The aim of this study is to solve the problems of extraction and purification of active components from PAF. Materials and Methods: The solvent to be used for extraction and its concentration, the solid-to-liquid ratio, and extraction duration were investigated using a single-factor experiment.An orthogonal design(L_(9)[3^(4)]) was used to determine the optimum extraction conditions. After optimization, the sample’s concentrations and flow velocity, the eluents and their velocity, adsorption time, and the removed water volume were measured. The content of the five steroids in the sample was determined by high-performance liquid chromatography(HPLC). We also investigated the anti-inflammatory property of PAF calyxes before and after purification. Results: The optimum extraction and purification processes were determined by single-factor analysis.AB-8 was identified as the best macroporous adsorption resin for enrichment. After optimization, the average total steroid content was 71.83%,and the average recovery was 90% after purification. Among the five steroid components detected by HPLC, physalin F showed the highest content. Furthermore, the sample obtained after purification could significantly inhibit paw edema by egg whites induced. Conclusions: An environmentally-sustainable, efficient, and stable process was first optimized for enriching and purifying total steroids from PAF. The process has the potential for further development and utilization in the pharmaceutical industry.展开更多
以总黄酮和总皂苷的综合评分为考察指标,采用Box-Behnken法优化提取工艺最佳的乙醇浓度、提取时间、料液比及浸泡时间。考察提取物的ABTS+及DPPH清除率及对在H 2 O 2诱导的氧化损伤B16细胞存活率,SOD、CAT和MDA水平评价其抗氧化活性。...以总黄酮和总皂苷的综合评分为考察指标,采用Box-Behnken法优化提取工艺最佳的乙醇浓度、提取时间、料液比及浸泡时间。考察提取物的ABTS+及DPPH清除率及对在H 2 O 2诱导的氧化损伤B16细胞存活率,SOD、CAT和MDA水平评价其抗氧化活性。结果显示,最佳提取工艺为:13.45倍量67.86%乙醇溶液浸泡0.89 h,回流1.68 h提取3次。锦灯笼提取物具有良好的抗氧化活性,可提高细胞CAT和SOD活性,降低MDA含量;提高氧化损伤状态的B16细胞的存活率,为深入研究锦灯笼提供实验基础。展开更多
文摘A new neophysalin, named 5α-hydroxy-25,27-dihydro-4,7-didehydro-7-deoxyneophysalin A（1）, along with three other known neophysalins （2-4） were isolated from the calyxes of Physalis alkekengi L. var.francheti （Mast.） Makino. The structure of 1 was determined by means of 1D and 2D NMR, UV, IR and mass spectra. Compound 1 displayed potent cytotoxicities in vitro against PC- 3 and LNCaP cell lines.
基金supported by the Guangzhou Science and Technology Program in 2020-Basic and Applied Basic Research Project(Grant No.202002030226)the National Natural Science Foundation of China(Grant No.82174043)the Guangdong Basic and Applied Basic Research Foundation(Grant No.2021A1515011697).
文摘Objective: As a traditional medicinal plant listed in the Chinese Pharmacopeia, Physalis alkekengi L. var. franchetii(Mast.) Makino(PAF) has a long medicinal history and high economic value. PAF has immunomodulatory properties and can be used to treat acute lung injury and eczema.The aim of this study is to solve the problems of extraction and purification of active components from PAF. Materials and Methods: The solvent to be used for extraction and its concentration, the solid-to-liquid ratio, and extraction duration were investigated using a single-factor experiment.An orthogonal design(L_(9)[3^(4)]) was used to determine the optimum extraction conditions. After optimization, the sample’s concentrations and flow velocity, the eluents and their velocity, adsorption time, and the removed water volume were measured. The content of the five steroids in the sample was determined by high-performance liquid chromatography(HPLC). We also investigated the anti-inflammatory property of PAF calyxes before and after purification. Results: The optimum extraction and purification processes were determined by single-factor analysis.AB-8 was identified as the best macroporous adsorption resin for enrichment. After optimization, the average total steroid content was 71.83%,and the average recovery was 90% after purification. Among the five steroid components detected by HPLC, physalin F showed the highest content. Furthermore, the sample obtained after purification could significantly inhibit paw edema by egg whites induced. Conclusions: An environmentally-sustainable, efficient, and stable process was first optimized for enriching and purifying total steroids from PAF. The process has the potential for further development and utilization in the pharmaceutical industry.
