Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from t...Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.展开更多
Objective:The compatibility of Eucommia ulmoides(Eu)and Psoralea corylifolia(Pc)on the pharmacokinetic(PK)properties in the rat was explored in this study.Methods:Eu extract,Pc extract and the combined extracts(crude ...Objective:The compatibility of Eucommia ulmoides(Eu)and Psoralea corylifolia(Pc)on the pharmacokinetic(PK)properties in the rat was explored in this study.Methods:Eu extract,Pc extract and the combined extracts(crude drug ratio was 2:1)was administered by gavage,respectively.Two PK experiments were conducted.In first one,the blood samples were collected via the occuli chorioideae vein to get the PK properties of the components.In second one,the blood samples were simultaneously collected via the internal jugular vein or portal vein at different time points and the concentrations of target ingredients were detected by LC/MS/MS to clear the location where the interaction of Eu and Pc took place in vivo.Results:Eight of 11 ingredients in Eu and Pc extract were determined in rat plasma.The exposure levels of geniposidic acid(GPA),aucubin(AU),geniposide(GP),pinoresinol diglucoside(PDG),psoralen glycosides(PLG)and isopsoralen glycosides(IPLG)were decreased 1/5–2/3 after administration of combined extracts.Comparing to the combined administration,the exposure of GPA and AU in plasma of single Eu administration collected via the portal vein were decreased 1/3–2/3,and the values of AUC0-24h and AUC0-∞ of GP collected from the portal vein or internal jugular vein were double increased.The other components’parameters were not significantly changed.Conclusion:In summary,the Pc and Eu combined administration could affect the exposure of the main components of Eu extract in rats due to the changed intestinal absorption.The research on the compatibility of Pc and Eu was helpful to guide the clinical administration of Eu and Pc simultaneously.展开更多
Psoraleae Fructus(PF)is an important traditional herbal medicine with a long history of clinical application.It is widely used to treat various diseases,such as osteoporosis,leucoderma and diarrhea.As a traditional no...Psoraleae Fructus(PF)is an important traditional herbal medicine with a long history of clinical application.It is widely used to treat various diseases,such as osteoporosis,leucoderma and diarrhea.As a traditional nontoxic herb,it has aroused worldwide concern about the potential risks due to increasing adverse reaction events.This article reviews the botany,ancient records of medical uses,adverse reactions,toxicological research advance and detoxification methods of PF.According to clinical studies,liver injury is the most predominant in PF-related adverse reactions.The underlying mechanisms include bile acid metabolism and transport disorders,oxidative stress,mitochondrial damage,inhibition of liver cell regeneration and inflammatory reactions.Furthermore,the potential toxins of PF are summarized.Traditional methods of processing and compatibility will provide reference for reducing the toxicity of PF,which requires further research.In sum,this work systematically summarizes the reserach progress on the safety of PF,which will provide comprehensive insights into the toxicity of PF and facilitate its safe use and future development.展开更多
AIM To isolate the chemical components from extracted crude of Psoralea corylifolia L. METHODS Applicate the function of molecular recognition in supramolecular chemistry, use 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 dio...AIM To isolate the chemical components from extracted crude of Psoralea corylifolia L. METHODS Applicate the function of molecular recognition in supramolecular chemistry, use 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol as the host molecule and chemical components in extracted crude of Psoralea corylifolia L. as guest molecules, which represent different from either in sort, quantity and sites of function groups or in structural topological character, 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol could selectively form inclusion compound with component endowed with interactional complementarity and isolate as crystalline from the extracted crude of Psoralea corylifolia L. RESULTS The isopsoralen as guest molecule is selectively included by 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol in inclusion crystal and removed by acetone from the inclusion compound and total yield is 0 18%. Isopsoralen is determined by UV, IR, 1HNMR and MS and its inclusion compound is determined by means of IR, powder XRD and single crystal XRD. The isolation effect is analyzed by GC/MS. CONCLUSION The method is simple and selective for isolation isopsoralen from extracted crude of Psoralea corylifolia L.展开更多
文摘Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.
基金This study was funded by Key Program for National Natural Science Foundation of China(No.81630106)National Natural Science Foundation of China(No.81803691).
文摘Objective:The compatibility of Eucommia ulmoides(Eu)and Psoralea corylifolia(Pc)on the pharmacokinetic(PK)properties in the rat was explored in this study.Methods:Eu extract,Pc extract and the combined extracts(crude drug ratio was 2:1)was administered by gavage,respectively.Two PK experiments were conducted.In first one,the blood samples were collected via the occuli chorioideae vein to get the PK properties of the components.In second one,the blood samples were simultaneously collected via the internal jugular vein or portal vein at different time points and the concentrations of target ingredients were detected by LC/MS/MS to clear the location where the interaction of Eu and Pc took place in vivo.Results:Eight of 11 ingredients in Eu and Pc extract were determined in rat plasma.The exposure levels of geniposidic acid(GPA),aucubin(AU),geniposide(GP),pinoresinol diglucoside(PDG),psoralen glycosides(PLG)and isopsoralen glycosides(IPLG)were decreased 1/5–2/3 after administration of combined extracts.Comparing to the combined administration,the exposure of GPA and AU in plasma of single Eu administration collected via the portal vein were decreased 1/3–2/3,and the values of AUC0-24h and AUC0-∞ of GP collected from the portal vein or internal jugular vein were double increased.The other components’parameters were not significantly changed.Conclusion:In summary,the Pc and Eu combined administration could affect the exposure of the main components of Eu extract in rats due to the changed intestinal absorption.The research on the compatibility of Pc and Eu was helpful to guide the clinical administration of Eu and Pc simultaneously.
基金supported by the National Natural Science Foundation of China (81320108029)the National “Major Scientific and Technological Special Project for Significant New Drugs” project (2015ZX09501004-002-004)Specific Fund for Public Interest Research of Traditional Chinese Medicine,Ministry of Finance (201507004-002)。
基金supported by the Major Program of National Natural Science Foundation of China(Nos.82192910 and 82192911).
文摘Psoraleae Fructus(PF)is an important traditional herbal medicine with a long history of clinical application.It is widely used to treat various diseases,such as osteoporosis,leucoderma and diarrhea.As a traditional nontoxic herb,it has aroused worldwide concern about the potential risks due to increasing adverse reaction events.This article reviews the botany,ancient records of medical uses,adverse reactions,toxicological research advance and detoxification methods of PF.According to clinical studies,liver injury is the most predominant in PF-related adverse reactions.The underlying mechanisms include bile acid metabolism and transport disorders,oxidative stress,mitochondrial damage,inhibition of liver cell regeneration and inflammatory reactions.Furthermore,the potential toxins of PF are summarized.Traditional methods of processing and compatibility will provide reference for reducing the toxicity of PF,which requires further research.In sum,this work systematically summarizes the reserach progress on the safety of PF,which will provide comprehensive insights into the toxicity of PF and facilitate its safe use and future development.
文摘AIM To isolate the chemical components from extracted crude of Psoralea corylifolia L. METHODS Applicate the function of molecular recognition in supramolecular chemistry, use 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol as the host molecule and chemical components in extracted crude of Psoralea corylifolia L. as guest molecules, which represent different from either in sort, quantity and sites of function groups or in structural topological character, 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol could selectively form inclusion compound with component endowed with interactional complementarity and isolate as crystalline from the extracted crude of Psoralea corylifolia L. RESULTS The isopsoralen as guest molecule is selectively included by 1,1,6,6 tetraphenylhexa 2,4 diyne 1,6 diol in inclusion crystal and removed by acetone from the inclusion compound and total yield is 0 18%. Isopsoralen is determined by UV, IR, 1HNMR and MS and its inclusion compound is determined by means of IR, powder XRD and single crystal XRD. The isolation effect is analyzed by GC/MS. CONCLUSION The method is simple and selective for isolation isopsoralen from extracted crude of Psoralea corylifolia L.
