Transformations of phosphatidylinositol phosphates in the outer and inner nuclear membrane are linked to synthesis and restitution of cellular membranes

The ultimate goal in phosphoinositides cellular metabolism is to decipher their global functional organization and coordination of the com- partmentalized signaling processes. In this report we present evidence linking nuclear phos- phoinositides cycle with endoplasmic reticulum synthesis and function. The rapid transformation of [3H]inositol-labeled phosphoinositides in the intact nuclei (IN) was captured in chase studies for 0-5 min, followed by examination of phosphatidylinositides in the inner nuclear me- mbrane (INM), the outer nuclear membrane (ONM) and endoplasmic reticulum (ER). We revealed that synthesis of phosphatidylinositol phosphates (PIPs) occurs in ONM and the de- phosphorylation takes place in the INM. The rapid transformation of the radiolabeled PIPs in ONM reverberated in their appearance and successive transformation in INM, and in the 5min chased nuclei was tracked to ONM as the re- emerging radiolabeled phosphatidylinositol (PI). These chase-uncovered changes in ONM and INM PIPs profiles allow us to conclude that the observed conversions in the nuclear membrane continuum are induced by the lateral movement of the membrane and its transit from the cytosolic to nuclear and back to cytosolic environment. The suggested membrane synthesisinduced movement provides the means to transport the membrane- and the membrane lipid ligand-associated cytosolic proteins to the intranuclear spaces and renewal of INM. Export of the nuclear components interacting with the modified INM, by exiting from nuclear to cytosolic site, endows ER with a steady influx of the membrane that is conditioned to generate vesicles according to the nucleus delivered templates.

Lipophilic extractives of the inner and outer barks from six different Pinus species grown in Indonesia

The chemical compositions of the dichloromethane extracts of inner and outer barks from six Pinus species（P.elliotii,P.oocarpa,P.caribeae,P.merkusii,P.montezumae,and P.insularis） grown in Indonesia were investigated by GC and GC–MS.Generally,the amounts of extractive contents were higher in the inner bark than in the outer bark except for P.merksuii.Fatty acids,monoterpenes,sesquiterpenes,resin acids,triterpenoids,and steroids were detected and quantified.Inner and outer barks differed not only in content of these compounds but also in their composition.Fatty acids and alcohols were the major classes of lipophilic compounds in the outer bark of P.caribeae, P.insularis,and P.montezumae.Steroids and triterpenoids were the dominant compounds identified in the inner bark of P.elliotii,P.insularis,and P.merkusii.Resin acids were the most abundant group in the inner bark of P.oocarpa whereas monoterpenes and sesquiterpenes were recorded in minor quantities in both bark layers of all species.

Synchronization investigation of the network group constituted by the nearest neighbor networks under inner and outer synchronous couplings

A new synchronization technique of inner and outer couplings is proposed in this work to investigate the synchro- nization of network group. Some Haken-Lorenz lasers with chaos behaviors are taken as the nodes to construct a few nearest neighbor complex networks and those sub-networks are also connected to form a network group. The effective node controllers are designed based on Lyapunov function and the complete synchronization among the sub-networks is realized perfectly under inner and outer couplings. The work is of potential applications in the cooperation output of lasers and the communication network.

Analysis and optimization of stamping and forming process of bearing outer ring

To address the common issues of wrinkling,tearing,and uneven wall thickness in the actual sheet metal stamp-ing process of the outer ring of needle roller bearings,this study analyzes critical technical indicators such asforming limits,thickness distribution,and principal strains in the forming process in detail.Three-dimensionalmodels of the concave and convex dies were constructed.The effects of different process parameters,includingstamping speed,edge pressure,sheet metal thickness,and friction coefficient,on the quality of the forming partswere investigated by varying these parameters.Subsequently,the orthogonal experimental method was used todetermine an optimal experimental group from multiple sets of experiments.It was found that under the processparameters of a stamping speed of 3000 mm/s,edge pressure of 2000 N,sheet metal thickness of 0.9 mm,andfriction coefficient of 0.125,the forming quality of the outer ring of the bearing is ideal.

Effect of the Initial Vortex Structure on Intensity Change During Eyewall Replacement Cycle of Tropical Cyclones:A Numerical Study

This study investigates the effect of the initial tropical cyclone(TC)vortex structure on the intensity change during the eyewall replacement cycle(ERC)of TCs based on two idealized simulations using the Weather Research and Forecasting(WRF)model.Results show that an initially smaller TC with weaker outer winds experienced a much more drastic intensity change during the ERC than an initially larger TC with stronger outer winds.It is found that an initially larger TC vortex with stronger outer winds favored the development of more active spiral rainbands outside the outer eyewall,which slowed down the contraction and intensification of the outer eyewall and thus prolonged the duration of the concentric eyewall and slow intensity evolution.In contrast,the initially smaller TC with weaker outer winds corresponded to higher inertial stability in the inner core and weaker inertial stability but stronger filamentation outside the outer eyewall.These led to stronger boundary layer inflow,stronger updraft and convection in the outer eyewall,and suppressed convective activity outside the outer eyewall.These resulted in the rapid weakening during the formation of the outer eyewall,followed by a rapid re-intensification of the TC during the ERC.Our study demonstrates that accurate initialization of the TC structure in numerical models is crucial for predicting changes in TC intensity during the ERC.Additionally,monitoring the activity of spiral rainbands outside the outer eyewall can help to improve short-term intensity forecasts for TCs experiencing ERCs.

Malfunction of outer retinal barrier and choroid in the occurrence and progression of diabetic macular edema

Diabetic macular edema(DME) is the most common cause of vision loss in diabetic retinopathy,affecting 1 in 15 patients with diabetes mellitus(DM).The disruption of the inner blood-retina barrier(BRB) has been largely investigated and attributed the primary role in the pathogenesis and progression in DME, but there is increasing evidence regarding the role of outer BRB, separating the RPE from the underlying choriocapillaris,in the occurrence and evolution of DME.The development of novel imaging technologies has led to major improvement in the field of in vivo structural analysis of the macula allowing us to delve deeper into the pathogenesis of DME and expanding our vision regarding this condition.In this review we gathered the results of studies that investigated specific outer BRB optical coherence tomography parameters in patients with DM with the aim to outline the current status of its role in the pathogenesis and progression of DME and identify new research pathways contributing to the advancement of knowledge in the understanding of this condition.

Subducting passive continental margins with crustal (ultra)mafic intrusions: An underappreciated mechanism for recycling water back into the mantle

It is well known that outer rise bending-assisted oceanic plate hydration is an important mechanism for transporting substantial amounts of water into the mantle.A key question is:Are there other equally or more important water transport mechanisms?Here we propose,for the first time,that subducting passive continental margins,particularly those with crustal(ultra)mafic intrusions,play a critical role in recycling water back into the mantle.Evidence for this mechanism is the exceptionally high outer rise seismicity observed in a subducting passive continental margin(i.e.,the northeastern South China Sea continental margin)near the northern Manila trench,characterized by a high-velocity lower crust that has been attributed to(ultra)mafic intrusions.Our interpretation of this correlation between high outer rise seismicity and lower crust(ultra)mafic intrusions is that(ultra)mafic intrusions alter the crustal rheology and increase brittle deformation in the lower crust in this region,thereby promoting lithospheric fracturing and plate hydration,which is evidenced by increased outer rise seismicity.

A Matrix Representation of Outer Derivations from gl0|2 to the Generalized Witt Lie Superalgebra

Let gl0|2 be a subalgebra of the general linear Lie superalgebra.In this paper,outer derivations from gl0|2 to the generalized Witt Lie superalgebra are completely determined by matrices.

Viscous Inner and Outer Pressure Forming Method of Thin-walled Tube and Its Application

Aiming at overcoming the difficulties in integral forming of thin-walled tubes with complex shapes, a novel forming method by inner and outer pressure through viscous was proposed. In this method, by dividing large deformation of the part into inner and outer pressure forming deformations, the limit deformation of tube part can be increased by several times. Meanwhile, the principle of viscous inner and outer pressure forming was provided, and key problems during the forming process such as reduction of the wall-thickness and instability wrinkling were analyzed. Thereby, the complex curved surface super-alloy GH3044 thin-walled tube with varying diameter ratio of 1.35（the ratio between the maximum and minimum diameters of the part） can be integrally formed by this method. The experimental surface of the formed part is superior in quality and the wall-thickness distribution is uniform. The results show that the viscous inner and outer pressure forming can provide a new approach for integral forming of thin-walled tubes with complex shapes.

Influence of Inner/Outer Stator Pole Ratio and Relative Position on Electromagnetic Performance of Partitioned Stator Switched Flux Permanent Magnet Machines

Based on the 6-pole outer stator(armature winding-stator),the influence of inner(permanent magnet-stator)/outer stator pole ratio n(n=NIS/NOS),stator relative positions and rotor pole number combinations on electromagnetic performance of partitioned stator switched flux permanent magnet(PM)machines(PS-SFPMMs)is investigated in this paper.Since the armature windings and PMs are located in two separated stators and PMs are stationary,PS-SFPMMs have high fault tolerance capabilities.To maximize the torque performance,the PM of inner stator pole should be aligned with outer stator pole when n is odd while the iron rib of inner stator pole should be aligned with outer stator pole when n is even.No matter what n is selected,the rotor pole number NR can be any integers except the phase number and its multiples.The analysis results indicate that the optimal NR is closed to(NIS+NOS)/2 and it is odd when n is odd while it is even when n is even.Meanwhile,symmetrical phase back-EMF waveform will be obtained when the ratio of Min(NOS,NIS)to the greatest common divisor of Min(NOS,NIS)and NR is even.Based on the optimal rotor pole numbers for 6-pole outer stator with different n and corresponding optimal relative position together with same rated copper loss,the average torque is improved by 18.4%,25.1%and 25.7%respectively in PS-SFPMMs with n equal to 2,3 and 4 when compared with PS-SFPMM with n equal to 1.The analyses are validated by experiment results of the prototype machine.

Inner and outer pressure forming of nickel based super-alloy thin-walled part with variable diameter sections

A novel forming method of nickel based super-alloy thin-walled part with variable diameter sections was proposed by using inner and outer pressure with the visco-elasto-plastic pressure-carrying medium at room temperature,and the principle of the method was provided.Experiments and FE simulations were carried out to analyze the deformation characteristics for the part with larger variable diameter ratio(35%).The results show that visco-elasto-plastic pressure-carrying medium can meet the requirements of the room-temperature deformation condition for nickel based super-alloy sheet.The inner and outer pressure forming with the visco-elasto-plastic pressure-carrying medium can meet the requirements of dimensional accuracy for the thin-walled part with variable diameter sections.The thinning of wall-thickness is less than 4%.This method provides a new approach for near-net shape forming of nickel based super-alloy thin-walled parts with variable diameter sections.

The Apple and the Ear, Grasping Sounds in Space—A Theory of Sound Localization

Sensing the direction of origin of a sound in space has long been attributed to the delay between arrival times between the two ears. This, now discredited two dimensional theory, was put to rest by the observation that a person deaf in one ear can locate sounds in three dimensional space. We present here a new theory of sound localization that has the re-quired three dimensional measurement. It is a theory that interprets the well researched biological structure of the mammalian cochlea in a new and logical way, which leads to a deeper understanding of how sound localization functions.

The Knee Joint Tissues Differ Significantly in TGFβ1 Expression and Its Sensitivity

The knee joint is the largest and most complex joint in the human body. In this study, we investigated TGFβ1 expression in the outer meniscus, inner meniscus and articular cartilage of rabbit and human knee tissue (outer and inner menisci) in order to determine the potential role of this factor in normal meniscal function. We also examined the potential of TGF-β1 stimulation to promote tissue regeneration in the two different regions of rabbit knee meniscus tissue. Immunohistochemical investigations of TGF-β1 were performed on rabbit and human knee tissue. The rabbit outer, inner and articular cartilage cells were culture and stimulated with TGF-β1 followed by cell proliferation assay and extracellular matrix analysis. Regulatory studies were performed using TGF-β1 inhibitors SB-431542 and PD98059. Gene expression was analyzed by quantitative polymerase chain reaction. We found marked regional variation in the expression of TGF-β1 in rabbit and human knee. TGF-β1 expressions are relatively greater in the outer meniscus than inner meniscus. Furthermore, we found that exogenous TGF-β1 stimulation increased cell proliferation and aggrecan synthesis more so in the outer than in the inner meniscus. Articular cartilage tissue shows moderate levels of cell proliferation and ECM synthesis when compared with outer and inner meniscus. These findings suggest that growth factors used to enhance the repair and regeneration of meniscal tissue should be tailored to enhance region-specific variation in cell proliferation and extracellular matrix synthesis.

Forward masking in distinguishing inner and outer hair cell damage

Forward Masking Temporal audiotory resolution is the ability of the auditory system to resolve auditory signals in the time domain. Forward masking is a means of studying temporal resolution where one tone, the probe, is masked by a preceding tone, the masker. Forward masking is

A Study on the Structural Response of a PSC Inner Containment Dome with Large Openings Due to Prestress Transfer

A linear three dimensional finite element (FE) study has been carried out to examine the structural response of a prestressed concrete (PSC) inner containment (IC) dome of reactor building (double containment system) of a typical Indian Nuclear Power Plant, having large steam generator (SG) openings with due emphasis on the local behaviour of the steel-concrete interfaces at the SG openings, due to initial prestress transfer. The predominant thrust of the study has been placed on the objective of predicting the possibilities of separation at the steel-concrete interface zones adjacent to the embedded plates (EPs) of the SG openings. Two types of modeling and analysis have been made to study the overall and local behaviour of the structure. Prestressing ducts, passive reinforcements and EPs have been included in the models in certain ways. For the FE analysis, the interface zone has been modeled using interface elements, the properties of which were derived from the results of past experiments conducted on steel plate-concrete inter-face specimens. The FE analysis results have been compared with the results of the past two FE analytical studies on the linear behaviour of the same PSC IC dome. Important observations have been made regarding dome deformation and stresses throughout the structure with special emphasis on the local behaviour of steel-concrete interfaces at and around the SG openings.

Moral&Intellectual Life of the West II Summary Outline of Christianity in the Modern World for Devout Christians and Skeptics

All human beings are born from divine mercy drawing man out of unfathomable reality’s chaotic depth into the divine realm’s peaceable light.The modern sense of justice,of fair government,depends on the teachings of Jesus to which all citizens must subscribe.This is not a mere ecclesiastic doctrine but an empirically proven fact.The imperfections of a finite albeit adaptable,very large computing system,which our brain appears to be,require us to be open to the infinite beyond,its recondite wisdom,for divinity to grant us a moral life&to come to our help.Jesus puts the individual human being center stage for the first time in history.The story of abounding divine mercy:Every day we humans struggle with the difficult moral law but Jesus taught us what matters most is our soul,St.Paul’s inner man,a biological function of our left brain.Relying upon the faith of the apostles,the Council of Chalcedon(AD 451)in its Confession affirmed that Jesus was an actual person in his substance,possessing two natures,human&divine.This widely has been interpreted to apply to all mankind,the soul of each human being,the inner man,is potentially divine.Forming a practical metaphysics for devout christians&skeptics:Metaphysics is needed for the purpose of undergirding faith.Origins of humanity in the deep structures of reality:Science appears to be opposite to our human spiritual existence&requires us to search for ourselves in the realm of the logos,that can serve as our guide,being one of the names of the Christ.Jesus was deeply influenced by the gifts of the Magi,brought to him from an Armenian revival during his age,source of charismatic theology,basic concepts&historical architecture.Survival by faith in the everyday human communal world:We must have a secure grasp of the Faith,its basics,memorized or divine help won’t come.Be a lifelong winner based upon your own inner man:The essential help from divinity,in our need,is granting us a moral life.Become a lifelong winner based upon your own inner man or woman.What it takes is a willing heart,a sudden inspiration.Version 1.Piety:To be aware of our marginal competence,nobody is born as a genetic loser.Version 2.Humility:To be ready for divine help,able to receive it,we need to take a step back.Version 3.Metanoia,the Ninety Degree Turn:Repentance,betterment is the beginning of faith for all of us.Miracles can&do happen,the down-&-out guide to happiness:Constant prayer is the effective remedy for a soul that has gone lost in the temptations of the human tribal world.

Seed and Wasp Production in the Mutualism of Figs and Fig Wasps

Figs (Moracea: Ficus) and fig wasps (Hymenoptera: Chlocloids: Agaonideae) depend on each other to complete their reproduction. Monoecious fig species and their pollinating wasps are in conflict over the use of fig ovaries which can either produce one seed or one wasp. From observation on Ficus virens Ait., we showed that female flowers with outer layer of ovaries (near to the wall of syconium) had no significant difference from that with inner and interval layer of ovaries (near to the syconium cavity), in which most seeds and wasps were produced. This meant that fig tree provided the same potential resource for seed and wasps production. Observation indicated that there was usually only one foundress in syconium at female flower phase and no com- petition pollinators. Measurement of the style length of female flowers and the ovipositor of pollinators indicated that most ovaries could be reached by pollinator’s ovipositor. However, at the male flower phase, production of seeds was significantly more than that of wasps including non-pollinating wasps but there was no significant difference between seed and pollinating wasp production when without non-pollinating wasps produced. This result indicated that non-pollinating wasps competed ovaries not with seeds but with pollinating wasps for ovipositing. Bagged experiment showed that the sampling fig species was not self-sterile which was important for figs and wasps to survive bad season. Seed production in self-pollinated figs was not significantly different from total wasps in- cluding non-pollinating ones. This might be related with the weaker competition among wasps since bagged figs were not easy to reach by wasps from outside.

The spanwise spectra in wall-bounded turbulence

The pre-multiplied spanwise energy spectra of streamwise velocity fluctuations are investigated in this paper. Two distinct spectral peaks in the spanwise spectra are observed in low-Reynolds-number wall-bounded turbulence. The spectra are calculated from direct numerical simulation （DNS） of turbulent channel flows and zero-pressure-gradient boundary layer flows. These two peaks locate in the nearwall and outer regions and are referred to as the inner peak and the outer peak, respectively. This result implies that the streamwise velocity fluctuations can be separated into large and small scales in the spanwise direction even though the friction Reynolds number Rer can be as low as 1000. The properties of the inner and outer peaks in the spanwise spec- tra are analyzed. The locations of the inner peak are invariant over a range of Reynolds numbers. However, the locations of the outer peak are associated with the Reynolds number, which are much higher than those of the outer peak of the pre-multiplied streamwise energy spectra of the streamwise velocity.

Characteristics of Corrosion Product Layer Formed on Weathering Steel Exposed to the Tropical Climate of Vietnam

The weathering steel (Corten B) was exposed to out-door atmosphere of Hanoi (urban site) and Donghoi (marine site). The results showed the protective ability of corrosion product layer formed on weathering steel in the initial stage. The SEM-EDX analysis detected the presence of chromium and copper in the inner layers of corrosion product formed on weathering steel. These elements improved corrosion resistance of corrosion product layers. In addition, the dense α-FeOOH phase were appeared early in corrosion product which is detected by X-ray diffraction and Micro Raman investigations. The results of polarization and EIS measurements also demonstrated the protectiveness of the corrosion product of weathering steel.

The principle of stability control of surrounding rock-bearing structures in high stress soft rock roadways

Through the description of the deformational features of the surrounding rockaround high stress engineering soft rock roadways,the coupling stabilization principle ofinner and outer structures in surrounding rock was put forward.The supporting principlesof high stress engineering soft rock roadway (high resistance and yielding support,timelysupport,high strength and high stiffness supports) were proposed,which were applied inengineering practices,and obtained better achievements.