[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to constru...[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments （1.0-5.0 kb） is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.展开更多
[Objective] This study aimed to investigate the effects of salt stress on seed germination and seedling growth in radish（Raphanus sativus L.）.[Method] First,effects of soaking seeds with clear water and different co...[Objective] This study aimed to investigate the effects of salt stress on seed germination and seedling growth in radish（Raphanus sativus L.）.[Method] First,effects of soaking seeds with clear water and different concentrations of saline solution were compared; then,different concentrations of salt solution （0.2％-1.0％） was used to treat radish during the whole germination period.[Result] soaking seeds with clear water can improve the germination indicators of radish seeds exposed to high salt stress; low salt concentration（0.2％,0.4％） had little effect on the growth indicators of radish seedlings,and 0.2％ salt concentration can even promote the growth of radish seedlings,however,the high salinity had a bad inhibition on seedling growth,the higher the salt concentration,the stronger the inhibition.[Conclusion] According to the comprehensive comparison,the order of the six varieties in salt-tolerance was as follows：Xinxiayuanbai＞W1029,Xiayuanbai ＞ Nanyangbailizhi,Chunyidianhong＞ Sijihong 3.展开更多
Carex brunnescens(Pers.)Poir.is considered to be the only clonal herb found to date that can develop and form fixed dunes in Maqu alpine degraded grasslands of northwestern China.However,due to strong dormant characte...Carex brunnescens(Pers.)Poir.is considered to be the only clonal herb found to date that can develop and form fixed dunes in Maqu alpine degraded grasslands of northwestern China.However,due to strong dormant characteristics of C.brunnescens seeds,the sand-fixing effect of the plant is severely limited.This study explores a technique that can rapidly promote the seed germination of C.brunnescens,and also investigates the adaptation and sand-fixing effect by cultivating C.brunnescens seedlings to establish living sand barriers in the sand ridges of moving sand dunes.Results show that the seed germination rate obtained a maximum of 63.7%or 65.1%when seeds were treated with 150 mg/L gibberellic acid(GA3)for 24 h followed by soaking in sulfuric acid(98%H2SO4)for 2.5 min or sodium hydroxide(10%NaOH)for 3.5 h,and then germinated(25°C in daytime and 5°C at nighttime)in darkness for 10 d.After breaking seed dormancy of C.brunnescens,the living sand barrier of C.brunnescens(plant spacing 15−20 cm;sand barrier spacing 10−20 m)was established in the perpendicular direction to the main wind in the middle and lower parts of the sand ridges on both sides of the moving sand dunes.When the sand ridges were leveled by wind erosion,the living sand barrier(plant spacing 15−20 cm;sand barrier spacing 0.5−1.0 m)of C.brunnescens was reestablished on the wind-eroded flat ground.Finally,a stable sand-fixing surface can be formed after connecting the living sand barriers on both sides,thus achieving a good sand-fixing effect.These findings suggest that rapid seed germination technology combined with the sand−fixing method of C.brunnescens can shorten the seed germination period and make the seedling establishment become much easier which may be an effective strategy to restore and reconstruct Maqu degraded grasslands.展开更多
基金Supported by Specialized Fund for the Basic Research Operating Expenses Program of International Centre for Bamboo and Rattan(163201300812618-7)Special Fund for Research and Development of Forestry Nonprofit Industry(200704001)~~
文摘[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments （1.0-5.0 kb） is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.
文摘[Objective] This study aimed to investigate the effects of salt stress on seed germination and seedling growth in radish（Raphanus sativus L.）.[Method] First,effects of soaking seeds with clear water and different concentrations of saline solution were compared; then,different concentrations of salt solution （0.2％-1.0％） was used to treat radish during the whole germination period.[Result] soaking seeds with clear water can improve the germination indicators of radish seeds exposed to high salt stress; low salt concentration（0.2％,0.4％） had little effect on the growth indicators of radish seedlings,and 0.2％ salt concentration can even promote the growth of radish seedlings,however,the high salinity had a bad inhibition on seedling growth,the higher the salt concentration,the stronger the inhibition.[Conclusion] According to the comprehensive comparison,the order of the six varieties in salt-tolerance was as follows：Xinxiayuanbai＞W1029,Xiayuanbai ＞ Nanyangbailizhi,Chunyidianhong＞ Sijihong 3.
基金the Project of the Youth Talent Development Fund of the Northwest Institute of Eco−Environment and ResourcesChinese Academy of Science(CAREERI)(Y851C81001)+1 种基金the National Natural Science Foundation of China(41877162)the Instrument Functional Development Project from the Technology Service Center of CAREERI(Y429C51007).
文摘Carex brunnescens(Pers.)Poir.is considered to be the only clonal herb found to date that can develop and form fixed dunes in Maqu alpine degraded grasslands of northwestern China.However,due to strong dormant characteristics of C.brunnescens seeds,the sand-fixing effect of the plant is severely limited.This study explores a technique that can rapidly promote the seed germination of C.brunnescens,and also investigates the adaptation and sand-fixing effect by cultivating C.brunnescens seedlings to establish living sand barriers in the sand ridges of moving sand dunes.Results show that the seed germination rate obtained a maximum of 63.7%or 65.1%when seeds were treated with 150 mg/L gibberellic acid(GA3)for 24 h followed by soaking in sulfuric acid(98%H2SO4)for 2.5 min or sodium hydroxide(10%NaOH)for 3.5 h,and then germinated(25°C in daytime and 5°C at nighttime)in darkness for 10 d.After breaking seed dormancy of C.brunnescens,the living sand barrier of C.brunnescens(plant spacing 15−20 cm;sand barrier spacing 10−20 m)was established in the perpendicular direction to the main wind in the middle and lower parts of the sand ridges on both sides of the moving sand dunes.When the sand ridges were leveled by wind erosion,the living sand barrier(plant spacing 15−20 cm;sand barrier spacing 0.5−1.0 m)of C.brunnescens was reestablished on the wind-eroded flat ground.Finally,a stable sand-fixing surface can be formed after connecting the living sand barriers on both sides,thus achieving a good sand-fixing effect.These findings suggest that rapid seed germination technology combined with the sand−fixing method of C.brunnescens can shorten the seed germination period and make the seedling establishment become much easier which may be an effective strategy to restore and reconstruct Maqu degraded grasslands.
