Objective:Systematically evaluating the efficacy and safety of adding and subtracting decoction of rehmanniae combined with Western medicine in the treatment of hypertension.Methods:PubMed,The Cochrane Library,EMBASE,...Objective:Systematically evaluating the efficacy and safety of adding and subtracting decoction of rehmanniae combined with Western medicine in the treatment of hypertension.Methods:PubMed,The Cochrane Library,EMBASE,Web of Science,CNKI,Wanfang Database,VIP database and China Biomedical Literature Service System(CBM)were searched.All randomized controlled studies(RCTs)on adding and subtracting decoction ofrehmanniae combined with Western medicine in the treatment of hypertension were retrieved.The retrieval period is from the database construction to November 2021.Two investigators independently screened the literature,collected baseline data of patients and data on related indicators,and evaluated the risk of bias in the included randomized controlled trials.Meta-analysis was performed by RevMan5.3 software.Results:Five randomized controlled trials including 380 patients were obtained after screening by inclusing and exclusing criteria.Meta-analysis results showed that:In the experimental group,TCM syndrome score(MD=-4.08,95%CI:-5.32 to-2.84,P<0.00001),systolic blood pressure(SBP)(MD=-7.77,95%CI:-12.03 to-3.51,P=0.0003),diastolic blood pressure(DBP)(MD=-6.76,95%CI:-9.59 to-3.93,P<0.00001)were better than the control group,and the differences were statistically significant.Conclusion:The current evidence shows that compared with the simple use of Western medicine,adding and subtracting decoction of rehmanniae combined with Western medicine in the treatment of hypertension has more advantages in improving TCM syndromes and reducing systolic and diastolic blood pressure,and the conclusion is reliable.However,due to the limited number and low quality of included randomized controlled trials,more randomized,double-blind,large-sample clinical studies are needed to confirm the above conclusions.展开更多
In this research,a new method based on the hyperspectral imaging for searching the best decocting time of sun dried ginseng is reported.The spectral images at diferent decocting time of test sample have been taken by ...In this research,a new method based on the hyperspectral imaging for searching the best decocting time of sun dried ginseng is reported.The spectral images at diferent decocting time of test sample have been taken by the st aring hyperspectral fAuorescence imaging systen and the solubility of active ingredients have been discussed by analyzing the changes on the spectral.curves.The spectr al range of the system is 400-720nm and the spectral resolution is 5nm.In the decocting process,the active ingredients of nonsoaked ginseng was dissolved in the tissue fluid at first,and reached equilibrium condition at last after the precipitation-dissolution reciprocating process of boiling.At last,the experiment al results show that the best decoction time of sun dried ginseng is about 60 min after boiling.展开更多
Objective:To study the mechanism of Yuanhuzhitong decoction for the treatment of osteoarthritis from the perspective of network pharmacology.Methods:The active ingredients of Yuanhuzhitong decoction were screened out ...Objective:To study the mechanism of Yuanhuzhitong decoction for the treatment of osteoarthritis from the perspective of network pharmacology.Methods:The active ingredients of Yuanhuzhitong decoction were screened out from the TCMSP database,and then the targets of the active ingredients were extracted.The drug-ingredient-target network was constructed using Cytoscape software.The genes of osteoarthritis were obtained by OMIM,GeneCards and DisGeNET databases.Drug targets and disease targets were mapped together,and the relationship of the key target protein-protein interaction(PPI)was obtained through the online STRING database.Key targets were analyzed by Clue GO plug-in for GO functional enrichment and KEGG signaling pathway enrichment.Results:A total of 71 active ingredients and 99 unique targets of alkaloids,coumarins,volatile oils,steroids,etc were obtained through the threshold of OB 30%and DL 0.18.45 common targets were obtained through Venn diagram,among which the core targets were IL6,VEGFA,CASP3,CCND1,ESR1,etc.GO enrichment analysis revealed that the biological processes of Yuanhuzhitong decoction for OA were mainly concentrated in cell migration,differentiation,nucleotide metabolism and synthesis,regulation of pri-miRNA transcription by RNA polymerase II,and apoptosis.KEGG analysis showed that Yuanhuzhitong decoction may act on HIF-1 signal pathway,TNF signal pathway,NF-κB signal pathway and other pathways.The results showed that the main active ingredients in Yuanhuzhitong decoction could play an important role for the treatment of osteoarthritis through"multi-component,multi-target and multi-pathway".Conclusion:This study analyzed the main active ingredients,key targets,signaling pathways and mechanism of Yuanhuzhitong decoction from the perspective of network pharmacology,laying a foundation for further exploration of its mechanism.展开更多
Traditional Chinese Medicine prescription Suanzaoren decoction (SZRD) is composed of Ziziphi Spinosae Semen, Chuanxiong Rhizoma, Anemarrhenae rhizoma, Poria and Licorice. It was used to treat central nervous system di...Traditional Chinese Medicine prescription Suanzaoren decoction (SZRD) is composed of Ziziphi Spinosae Semen, Chuanxiong Rhizoma, Anemarrhenae rhizoma, Poria and Licorice. It was used to treat central nervous system diseases such as insomnia and anxiety for thousands of years. This paper aims to systematically understand varieties and quantities of compounds and clarify chemical components of SZRD, subsequently to further provide the reference for phytochemistry and pharmacology researches of SZRD. Our results showed that SZRD contained 145 components, including flavonoids, triterpenoids, steroids, coumarins, phthalides, and volatile oils, etc., while five-single herbs contain 1104 components. Only in terms of compound number, there were 80 common components in SZRD and its five herbs, which accounted for 6.8% of total compounds in all 5 herbs and 55.2% of compounds in SZRD. The components of SZRD were not simply the sum of one in every single herb. It is necessary to perform parallel studies among SZRD and its herbs. This review discussed the problems that existed in the chemical research of SZRD and pointed out the direction for its further research.展开更多
为研究厚朴三物汤治疗腹痛的作用机制,通过网络药理学和分子对接技术探究了厚朴三物汤的活性成分、作用靶点及信号通路机制。首先使用相关软件筛选厚朴三物汤的活性成分及腹痛相关靶点,接着利用绘图工具构建“药物成分-靶点”网络图及...为研究厚朴三物汤治疗腹痛的作用机制,通过网络药理学和分子对接技术探究了厚朴三物汤的活性成分、作用靶点及信号通路机制。首先使用相关软件筛选厚朴三物汤的活性成分及腹痛相关靶点,接着利用绘图工具构建“药物成分-靶点”网络图及蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,然后使用用于注释、可视化和集成发现的数据库(database for annotation,visualization and integrated discovery,DAVID)进行生物信息学富集分析,最后进行蛋白分子对接。结果表明,厚朴三物汤治疗腹痛的核心蛋白为AKT1、表皮生长因子受体(epidermal growth factor receptor,EGFR)、ESR1、BCL2等;有效活性成分为3,4,5,7-四甲氧基黄酮、三甲氧基黄酮、木犀草素、香蜂草苷、异橙黄酮和去氢二松柏醇等;涉及的信号通路包括癌症相关途径、EGFR耐药性、HIF-1信号通路等。分子对接结果揭示了活性成分与靶点之间的高效结合潜力,厚朴三物汤可通过多成分、多靶点、多途径发挥治疗腹痛的作用。该研究为厚朴三物汤的临床应用提供了依据。展开更多
文摘Objective:Systematically evaluating the efficacy and safety of adding and subtracting decoction of rehmanniae combined with Western medicine in the treatment of hypertension.Methods:PubMed,The Cochrane Library,EMBASE,Web of Science,CNKI,Wanfang Database,VIP database and China Biomedical Literature Service System(CBM)were searched.All randomized controlled studies(RCTs)on adding and subtracting decoction ofrehmanniae combined with Western medicine in the treatment of hypertension were retrieved.The retrieval period is from the database construction to November 2021.Two investigators independently screened the literature,collected baseline data of patients and data on related indicators,and evaluated the risk of bias in the included randomized controlled trials.Meta-analysis was performed by RevMan5.3 software.Results:Five randomized controlled trials including 380 patients were obtained after screening by inclusing and exclusing criteria.Meta-analysis results showed that:In the experimental group,TCM syndrome score(MD=-4.08,95%CI:-5.32 to-2.84,P<0.00001),systolic blood pressure(SBP)(MD=-7.77,95%CI:-12.03 to-3.51,P=0.0003),diastolic blood pressure(DBP)(MD=-6.76,95%CI:-9.59 to-3.93,P<0.00001)were better than the control group,and the differences were statistically significant.Conclusion:The current evidence shows that compared with the simple use of Western medicine,adding and subtracting decoction of rehmanniae combined with Western medicine in the treatment of hypertension has more advantages in improving TCM syndromes and reducing systolic and diastolic blood pressure,and the conclusion is reliable.However,due to the limited number and low quality of included randomized controlled trials,more randomized,double-blind,large-sample clinical studies are needed to confirm the above conclusions.
文摘In this research,a new method based on the hyperspectral imaging for searching the best decocting time of sun dried ginseng is reported.The spectral images at diferent decocting time of test sample have been taken by the st aring hyperspectral fAuorescence imaging systen and the solubility of active ingredients have been discussed by analyzing the changes on the spectral.curves.The spectr al range of the system is 400-720nm and the spectral resolution is 5nm.In the decocting process,the active ingredients of nonsoaked ginseng was dissolved in the tissue fluid at first,and reached equilibrium condition at last after the precipitation-dissolution reciprocating process of boiling.At last,the experiment al results show that the best decoction time of sun dried ginseng is about 60 min after boiling.
基金Capacity building project of Chinese and western medicine clinical collaboration on major difficult disease in 2019-Stepwise therapies of integrated Chinese and western medicine for knee osteoarthritis.
文摘Objective:To study the mechanism of Yuanhuzhitong decoction for the treatment of osteoarthritis from the perspective of network pharmacology.Methods:The active ingredients of Yuanhuzhitong decoction were screened out from the TCMSP database,and then the targets of the active ingredients were extracted.The drug-ingredient-target network was constructed using Cytoscape software.The genes of osteoarthritis were obtained by OMIM,GeneCards and DisGeNET databases.Drug targets and disease targets were mapped together,and the relationship of the key target protein-protein interaction(PPI)was obtained through the online STRING database.Key targets were analyzed by Clue GO plug-in for GO functional enrichment and KEGG signaling pathway enrichment.Results:A total of 71 active ingredients and 99 unique targets of alkaloids,coumarins,volatile oils,steroids,etc were obtained through the threshold of OB 30%and DL 0.18.45 common targets were obtained through Venn diagram,among which the core targets were IL6,VEGFA,CASP3,CCND1,ESR1,etc.GO enrichment analysis revealed that the biological processes of Yuanhuzhitong decoction for OA were mainly concentrated in cell migration,differentiation,nucleotide metabolism and synthesis,regulation of pri-miRNA transcription by RNA polymerase II,and apoptosis.KEGG analysis showed that Yuanhuzhitong decoction may act on HIF-1 signal pathway,TNF signal pathway,NF-κB signal pathway and other pathways.The results showed that the main active ingredients in Yuanhuzhitong decoction could play an important role for the treatment of osteoarthritis through"multi-component,multi-target and multi-pathway".Conclusion:This study analyzed the main active ingredients,key targets,signaling pathways and mechanism of Yuanhuzhitong decoction from the perspective of network pharmacology,laying a foundation for further exploration of its mechanism.
文摘Traditional Chinese Medicine prescription Suanzaoren decoction (SZRD) is composed of Ziziphi Spinosae Semen, Chuanxiong Rhizoma, Anemarrhenae rhizoma, Poria and Licorice. It was used to treat central nervous system diseases such as insomnia and anxiety for thousands of years. This paper aims to systematically understand varieties and quantities of compounds and clarify chemical components of SZRD, subsequently to further provide the reference for phytochemistry and pharmacology researches of SZRD. Our results showed that SZRD contained 145 components, including flavonoids, triterpenoids, steroids, coumarins, phthalides, and volatile oils, etc., while five-single herbs contain 1104 components. Only in terms of compound number, there were 80 common components in SZRD and its five herbs, which accounted for 6.8% of total compounds in all 5 herbs and 55.2% of compounds in SZRD. The components of SZRD were not simply the sum of one in every single herb. It is necessary to perform parallel studies among SZRD and its herbs. This review discussed the problems that existed in the chemical research of SZRD and pointed out the direction for its further research.
文摘为研究厚朴三物汤治疗腹痛的作用机制,通过网络药理学和分子对接技术探究了厚朴三物汤的活性成分、作用靶点及信号通路机制。首先使用相关软件筛选厚朴三物汤的活性成分及腹痛相关靶点,接着利用绘图工具构建“药物成分-靶点”网络图及蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,然后使用用于注释、可视化和集成发现的数据库(database for annotation,visualization and integrated discovery,DAVID)进行生物信息学富集分析,最后进行蛋白分子对接。结果表明,厚朴三物汤治疗腹痛的核心蛋白为AKT1、表皮生长因子受体(epidermal growth factor receptor,EGFR)、ESR1、BCL2等;有效活性成分为3,4,5,7-四甲氧基黄酮、三甲氧基黄酮、木犀草素、香蜂草苷、异橙黄酮和去氢二松柏醇等;涉及的信号通路包括癌症相关途径、EGFR耐药性、HIF-1信号通路等。分子对接结果揭示了活性成分与靶点之间的高效结合潜力,厚朴三物汤可通过多成分、多靶点、多途径发挥治疗腹痛的作用。该研究为厚朴三物汤的临床应用提供了依据。
