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Exploration on the Characteristics and Important Relationships of Xi Jinping's New Concept of Coordinated Economic Development
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作者 Huang Shaoqin Sun Peng Wu Hao 《Contemporary Social Sciences》 2017年第4期96-108,共13页
During the 18 th CPC National Congress, Xi Jinping raised a series of economic development thoughts, namely the five new concepts of development; innovative, coordinated, green, open and shared development, and put fo... During the 18 th CPC National Congress, Xi Jinping raised a series of economic development thoughts, namely the five new concepts of development; innovative, coordinated, green, open and shared development, and put forward a series of economic development thoughts including"people-centered"economic development. These are the new summations of the practice of socialism with Chinese characteristics, and the latest achievements in the theory of the political economics of socialism with Chinese characteristics."People-centered"is both the starting point and final purpose of coordinated economic development, an accurate mastery of social and economic development rules, and also the foundation and specific manifestation of coordinated development between people and society. People's subjectivity, coordinated inclusiveness and dialectics of development are the characteristics of coordinated economic development. Regional coordinated development, urbanrural coordinated development, industrial coordinated development, coordinated development of national defense and economic construction, supply and demand coordinated development, ecological coordinated development and interacted coordinated development of the global economy are the important relationships of coordinated economic development that we have to recognize and handle properly. 展开更多
关键词 Xi Jinping’s new concept of coordinated development new feature important relationships
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A Comparative Study Of New Criticism&Semiotic Criticism——In Terms Of Their View Of The Relationship Between The Fictional World&The Real World
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作者 Chen Hong-Qin 《宜宾学院学报》 1997年第4期53-57,共5页
As we know,new criticism is the post—world school of critical theory insists onthe intrinsic value of work of art and focusses on the individual work alone asan independent unit of meaning.It is opposed to the critic... As we know,new criticism is the post—world school of critical theory insists onthe intrinsic value of work of art and focusses on the individual work alone asan independent unit of meaning.It is opposed to the critical practice of bringing histori-cal of brographical data to bear on the anterpretation of a work.To the new critocs,poet-.ry was a special kind of discourse,a means of communicating feeling and thought thatwould not be expressed in any other kind of language.It differed qualitatively from thelanguage of science,or philosophy,but it converyed valid meanings.Such critics set outto define and formalize the qualities of poetic thought and languagege,using the techniqueof close reading with special emphasis on the implicative values of works and on the vari-ous function of figurative language—imagery,metaphor and symbol—in the work. 展开更多
关键词 World A Comparative Study Of New Criticism&Semiotic Criticism VIEW In Terms Of their View Of the relationship between the Fictional World&the real World
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RELATIONSHIP BETWEEN MAN AND NATURE IN TRADITIONAL HUMAN RIGHTS CONCEPTS OF CHINA AND WEST
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作者 MENG PEIYUAN 《The Journal of Human Rights》 2003年第1期6-9,共4页
As the process of economic globalization gathers momentum, dialogue between different cultures has become extremely important. There should be dialogue between the Chinese and Western cultures under the principle of e... As the process of economic globalization gathers momentum, dialogue between different cultures has become extremely important. There should be dialogue between the Chinese and Western cultures under the principle of equality and mutual respect and understanding to increase consensus for mutual development. 展开更多
关键词 that MAN relationship between MAN and NATURE IN TRADITIONAL HUMAN RIGHTS conceptS OF CHINA and WEST of for in
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Changing Concepts of City and Urban Planning Practices in Guangzhou(1949–2010): An Approach to Sustainable Urban Development 被引量:2
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作者 XUE Desheng HUANG Gengzhi +1 位作者 GUAN Jingwen LIN Jiarong 《Chinese Geographical Science》 SCIE CSCD 2014年第5期607-619,共13页
By focusing on Guangzhou, this paper examines how different concepts of city in terms of urban-rural relationships are shaped in transforming China since 1949, and explores how they influence the way in which urban pl... By focusing on Guangzhou, this paper examines how different concepts of city in terms of urban-rural relationships are shaped in transforming China since 1949, and explores how they influence the way in which urban planning are practiced. In the pre-reform era, the city was defined as a productive space under the system of urban-rural segregation established for securing rapid industrialization. Urban planning was thus only concerned with the development of urban areas. In the 1980 s and 1990 s after the reform, institutional change and economic transformation mobilized the urban-rural linkages. The development of suburban areas was then considered by urban planning, but this consideration was a passive response to urban problems such as population explosion and water resources protection. Since the new millennium, the agenda for sustainable development and problems of uneven urban-rural development have called for the development of urban-rural integration. In this context, the city is conceptualized as a complex of mountain, city, river, farmland and sea, reconstructing the ideology of urban-rural division in defining urban development. Urban planning has therefore sought in an active way to develop a sustainable city embracing rural and natural elements, and to balance economic growth and environmental protection. It is argued that developing the concept of city as a complex of urban and rural elements contributes to the urban planning for sustainable urban development, while this conceptualization relies on the recognition of the integrated urban-rural relationship. 展开更多
关键词 urban-rural relationship concept of city urban planning sustainable urban development GUANGZHOU
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Innovation and practice in the service model of the nursing team in the 37◦C comprehensive ward
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作者 Ruijuan Huang Yuxin Zhang Guanfeng Fu 《Discussion of Clinical Cases》 2023年第3期6-11,共6页
Objective:To establish a nursing service model of 37◦C“six-center service”and study the effect of this model on improving nursing services in the course of nursing work.Methods:The semi-structured interview method a... Objective:To establish a nursing service model of 37◦C“six-center service”and study the effect of this model on improving nursing services in the course of nursing work.Methods:The semi-structured interview method and group meeting method are used to establish the 37◦C nursing service model and implement the“general health”concept of“benevolence-based and central enterprise quality.”The newly established 37◦C“six-center service”(enthusiastic reception,patient explanation,careful observation,sincere help,warm-hearted delivery and heart-to-heart visit)nursing model in the comprehensive ward of Baogang Hospital(Inner Mongolia)runs through the three phases of patient admission,hospitalization and discharge,providing patients with warm care services,so that patients can truly enjoy the warmth of 37◦C nursing and friendly family flair during the peri-hospitalization period.Results:(1)The established 37◦C“six-heart service”nursing service model has been treated as a nursing service path and is widely used in the hospital;(2)After the implementation of the 37◦C nursing service,patients,nurses,doctors,hospitals and the society show an effectively improved satisfaction;(3)The 37◦C“six-heart service”nursing service model is established as a hospital nursing service brand;(4)The 37◦C“six-heart service”nursing service brand has received enthusiastic responses in Baotou,Health Management Technology Co.,Ltd.and GENERTEC.Conclusions:The established 37◦C“six-heart service”nursing service model can effectively improve the quality of nursing service and establish a nursing service brand,which can be promoted across the country. 展开更多
关键词 Nursing service concept Nurse-patient relationship Innovative nursing practices
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Molecular Characterization and mRNA Expression of Cytochrome P450 1A1 and Cytochrome P450 3A in Liver of Kafue Lechwe (<i>Kobus leche kafuensis</i>) as Potential Biomarkers of Pollution of the Kafue River Basin, Zambia
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作者 Ethel M’kandawire Michelo Syakalima +6 位作者 Kaampwe Muzandu Girja Pandey Martin Simuunza Shouta M. M. Nakayama Yusuke K. Kawai Yoshinori Ikenaka Mayumi Ishizuka 《Engineering(科研)》 2014年第2期51-58,共8页
The aim of this study was to characterize CYP1A1 and CYP3A in liver of the Kafue lechwe from Lochnivar and Blue Lagoon GMAs and to determine their mRNA expression levels as potential biomarkers of pollution. Increasin... The aim of this study was to characterize CYP1A1 and CYP3A in liver of the Kafue lechwe from Lochnivar and Blue Lagoon GMAs and to determine their mRNA expression levels as potential biomarkers of pollution. Increasing attention is paid to the problem of pesticide pollution by people. Risk assessment of pesticide should not be only based on the chemical analysis of environmental samples, since this method could not truly distinguish whether the pesticide was harmful to environment. Thus, biological assessment should be established. 展开更多
关键词 Lochnivar GAME MANAGEMENT Area Blue Lagoon GAME MANAGEMENT Area Pestcides/Herbicides BIOMONITORING Phylogenetic relationships real-Time RT-PCR
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The Cross-Correlation Relationship Between the Real Estate Industry and High-Quality Economic Development
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作者 Mengwan ZHANG Fengfeng GAO +2 位作者 Jialin GAO Weiguo CHEN Chong Aik LEE 《Journal of Systems Science and Information》 CSCD 2021年第6期704-720,共17页
Based on China’s real estate investment(REI), commercial housing sales(CHS), and GDP data from 2001 to 2020, we studied the dependence, asymmetry, and transmission direction of China’s real estate and economy. Their... Based on China’s real estate investment(REI), commercial housing sales(CHS), and GDP data from 2001 to 2020, we studied the dependence, asymmetry, and transmission direction of China’s real estate and economy. Their cross-correlation is demonstrated through the deep crosscorrelation analysis(DCCA) algorithm, multifractal asymmetric detrended cross-correlation analysis(MF-ADCCA) algorithm and DCCA algorithm based on a time delay. Both REI and CHS have persistence with GDP. The real estate industry and economy have asymmetric multifractal characteristics.Meanwhile, real estate and the economy have different trends, and they can affect each other with the same length of time delay. This effect diminishes in a short period of time, and there are unsteady fluctuations in cross-correlation persistence in the long term. 展开更多
关键词 real estate industry high-quality economic development cross-correlation relationship
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总体国家安全观视域下中华民族共同体意识的逻辑展开
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作者 张洪 李雨萌 《中国人民警察大学学报》 2025年第1期61-69,共9页
加强中华民族共同体意识与总体国家安全观之间逻辑关系的研究,对丰富国家安全理论有重要作用。在表层逻辑上,二者存在相互促进与相互依存两种关系;在深层逻辑上,可以从哲学与国家战略两方面深入探讨二者之间的关系。在厘清中华民族共同... 加强中华民族共同体意识与总体国家安全观之间逻辑关系的研究,对丰富国家安全理论有重要作用。在表层逻辑上,二者存在相互促进与相互依存两种关系;在深层逻辑上,可以从哲学与国家战略两方面深入探讨二者之间的关系。在厘清中华民族共同体意识与总体国家安全观之间逻辑关系的基础上,通过构建总体国家安全观视域下中华民族共同体意识的理论框架,完善总体国家安全观视域下中华民族共同体意识的政策和法律支持,丰富总体国家安全观视域下中华民族共同体意识的实践路径,可以更好地促进中华民族共同体意识的增强,加强对国家安全的维护。 展开更多
关键词 中华民族共同体意识 总体国家安全观 国家安全 逻辑关系
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基于Android系统的学习平台应用
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作者 张鹏飞 《绵阳师范学院学报》 2014年第2期103-106,共4页
研究应用并评测了一个基于Android系统的实时学习平台,为学生提供一种新的学习手段。系统是基于学生体验设计的学习系统,包括在线学习、测验、音频、视频播放、双向信息系统、协同概念地图学习系统等功能。学生可以利用本学习系统,进行... 研究应用并评测了一个基于Android系统的实时学习平台,为学生提供一种新的学习手段。系统是基于学生体验设计的学习系统,包括在线学习、测验、音频、视频播放、双向信息系统、协同概念地图学习系统等功能。学生可以利用本学习系统,进行情境式的移动学习,整体环境让学生有良好的学习体验,而所有学习信息都记录在学习管理系统中,这也更能方便教师制定教学计划。经实际使用验证,本系统能有效的提高学生的学习效率,改善学生的学习体验。 展开更多
关键词 andROID 实时学习 情境学习 概念地图
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Enigma of Exam-oriented Education in China:A comparison on Sino-U.S.Educational Concepts in Higher Education
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作者 何爱玲 杨坚 《海外英语》 2013年第1X期99-101,共3页
College English teaching in China has long been criticized for its orientation to examination that consequently brings about negative learning attitude and ineffective learning achievement.Chinese educationists have b... College English teaching in China has long been criticized for its orientation to examination that consequently brings about negative learning attitude and ineffective learning achievement.Chinese educationists have brought forward several ap proaches to solve this problem but little has been changed due to the cultural influences.This paper analyzes the roots of examoriented education system in China in perspective of culture by comparing the Sino-U.S.cultural elements in depth in tradition al culture,learning and teaching motivation,teacher-student relationship and learners'engagement in class.This paper finally poses two suggestions on how to change the current situation gradually:(1) Focus on the washback of evaluation system on edu cational concepts.(2) Transform the product-evaluation to process-evaluation gradually by standardizing the current evaluation approaches,especially the standards of process-evaluation. 展开更多
关键词 Sino-U.S.teaching concepts motivation teacher-stud
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The Principle of Calibration Using the Ionic Current——A New Calibration Method for Electro-Magnetic Flowmeters
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作者 Li Bin Sheng Yu (School of Automation) 《Advances in Manufacturing》 SCIE CAS 1998年第2期38-42,共5页
An equivalent relationship between electro magnetic flowmeters (EMFs) and the Hall effect is discussed in principle and three equivalent conclusions about their measuring equations are derived. Thereby,the calibratio... An equivalent relationship between electro magnetic flowmeters (EMFs) and the Hall effect is discussed in principle and three equivalent conclusions about their measuring equations are derived. Thereby,the calibration using the ionic current (CUIC) is introduced to the calibration of EMFs in a special Hall effect system. A basic system with these equations is specially given and discussed for realizing the method. Two key points about CUIC are proved by a simple experiment. 展开更多
关键词 calibration of EMFs equivalent relationship between EMFs and Hall effect ionic current being equivalent to the real flowrate of an EMF
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Partnership in nursing care: a concept analysis
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作者 Si-Yan Yuan Joan Murphy 《TMR Integrative Nursing》 2019年第1期21-26,I0002,共7页
Objectives: This concept analysis aims to provide a better understanding of the term “partnership” in nursing care. A critical review of empirical studies regarding this area, can lay the groundwork for improving pa... Objectives: This concept analysis aims to provide a better understanding of the term “partnership” in nursing care. A critical review of empirical studies regarding this area, can lay the groundwork for improving partnership between nurses and clients, as well as person- centered nursing care. Methods: A comprehensive literature search used various “partnership”,“nurse”and “clients”search terms to identify relevant studies published from 2007 to 2017. The selected studies had to focus on partnership and nursing care. Walker and Avant’(2004) framework was used when synthesizing the evidence from empirical studies in the concept analysis. It helps to distinguish confusing or unclear concepts, and to promote critical thinking through analyzing its attributes, operational definitions, antecedents, consequences, and reference. Results: In nursing care, partnership is the relationship based on collaboration among individuals, who place trust and confidence in accountability, and who have capability and empathy. The antecedents are health problems or requirements, willingness to sign up as partners and ongoing commitment;while the consequences were presented as improvement in quality of service, treatment outcomes, clients’ safety and satisfaction, financial benefit as well as time constraints and professionals’ emotional burden. The Patient Partnership in Care questionnaire can help assess partnership in nursing care. Conclusion: This concept analysis informs the concept of partnership in nursing care by using Walker and Avant’(2004) framework. By synthesizing the evidence from empirical studies, it provides theoretical and clinical frameworks for nurses to innovate nurse-client relationships - nurses are seen as partners in care instead of experts, which can encourage clients’ self-management and improve person-centered nursing care. 展开更多
关键词 PARTNERSHIP Nursing care relationship concept analysis
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近代翻译文学:教育观念转变的“引擎”
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作者 李震 《教育学报》 CSSCI 北大核心 2024年第4期184-194,共11页
鸦片战争以降,西学翻译和教育变革成为国人呼声最高的两剂救亡药方。对于翻译中伴随的异质性,如何作用于教育转型的进程,进而引领着教育观念的转变,值得寻绎。潮水般涌入华夏大地的泰西文学,促使近代人才观不断位移,各类译著中的西方人... 鸦片战争以降,西学翻译和教育变革成为国人呼声最高的两剂救亡药方。对于翻译中伴随的异质性,如何作用于教育转型的进程,进而引领着教育观念的转变,值得寻绎。潮水般涌入华夏大地的泰西文学,促使近代人才观不断位移,各类译著中的西方人物,焕发出别具一格的魅力,为国人树立了一种新型人才“标杆”。西方教育小说中的教师,呈现出与传统中国教师截然不同的面相,成为形塑崭新师生关系的直接资源。在翻译小说的浸染之下,通俗文学逐渐深入人心,并在课堂上下、校园内外枝蔓攀升、花蕊广播。国人的知识结构在翻译文学的影响之下进行重组,中国教育模式得以吐故纳新,新的教育生态也逐渐建构起来。 展开更多
关键词 近代翻译文学 教育观念 人才观 师生关系 通俗教育
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新形势下中国对外话语的新天下观与国际传播的优化
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作者 李志凌 《昆明学院学报》 2024年第1期107-116,共10页
面对当今日益复杂的国际形势,中国的对外交往面临着一系列严峻挑战,在对外话语、国际传播等问题上需有与之相适的措施。由中国传统文化发展而来的新“天下观”,以其独特的政治哲学视角,为我们认清世界格局、处理国际关系打开了新的思路... 面对当今日益复杂的国际形势,中国的对外交往面临着一系列严峻挑战,在对外话语、国际传播等问题上需有与之相适的措施。由中国传统文化发展而来的新“天下观”,以其独特的政治哲学视角,为我们认清世界格局、处理国际关系打开了新的思路,也从认识论和方法论的角度,为对话世界提供了框架式构想。“天下”观的理论精神与构建“人类命运共同体”的基本理念一致,以实现世界各国人民的共同利益、和谐关系和携手发展为根本目的,从国际关系治理入手,以文明共生为目的,将政治生活和文化关系作为抓手,为我国的对外交流和文化传播设计出了系统化的工作格局和实施方案。 展开更多
关键词 新天下观 国际关系 对外话语 文化传播 表达路径
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周·三叩大门问地震——漫步地震五千年(4)
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作者 冯锐 《地震科学进展》 2024年第2期175-186,共12页
公元前780年的岐山地震是中国历史有明确纪年后的第一个震例,古代思想家没有停留于地震破坏的表面现象,而在探索地震规律上迈出了原始的第一步:伯阳父首次提出了阴阳斗争的地震机理,晏子注意了地震天象的关系,庄子指出了地震复发的特点... 公元前780年的岐山地震是中国历史有明确纪年后的第一个震例,古代思想家没有停留于地震破坏的表面现象,而在探索地震规律上迈出了原始的第一步:伯阳父首次提出了阴阳斗争的地震机理,晏子注意了地震天象的关系,庄子指出了地震复发的特点。本文从这3方面的地震实例,介绍了现代科学的认识和发展,最后简介了可公度性的基本概念。 展开更多
关键词 岐山地震 阴阳斗争 地震天象 地震复发
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马克思主义哲学究竟在何种意义上是“改变世界”的哲学
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作者 赵小狮 《运城学院学报》 2024年第6期1-6,共6页
马克思主义哲学之所以是区别于“解释世界”的旧哲学的“改变世界”的哲学,在于实践观点在世界观意义上的全面确立。以现实的人的实践活动为基点,从根本上变革了对把握世界的根据的理解,变革了对现实物质世界存在性质的理解,变革了对现... 马克思主义哲学之所以是区别于“解释世界”的旧哲学的“改变世界”的哲学,在于实践观点在世界观意义上的全面确立。以现实的人的实践活动为基点,从根本上变革了对把握世界的根据的理解,变革了对现实物质世界存在性质的理解,变革了对现实物质世界逻辑主体的理解,变革了人们关于物质的传统观念。确认现实世界既不是某种超验的精神运动过程,也不是自在的物质运动过程,而是由人的物质实践活动构成的世界,是现实的人以先在自然为前提改变世界创造历史的辩证发展过程。实践观点是马克思主义哲学的核心观点,实践概念才是马克思主义哲学的理论基石和逻辑起点。 展开更多
关键词 现实的人 现实物质世界 实践逻辑 新唯物主义物质观念
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基于行为决策理论的房产买卖双方购买决策差异研究:评估模式对房产偏好和估值的影响
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作者 张秀智 陆同源 《中国房地产金融》 2024年第6期55-64,共10页
房产购置是大部分消费者一生中最为重要的复杂决策之一。本文基于行为决策理论的视角和范式,对正在参与房产决策过程的买卖双方和模拟参与房产交易的被试进行房产偏好和估值调查(N=1038),研究了房地产买卖双方的评估模式、评估模式产生... 房产购置是大部分消费者一生中最为重要的复杂决策之一。本文基于行为决策理论的视角和范式,对正在参与房产决策过程的买卖双方和模拟参与房产交易的被试进行房产偏好和估值调查(N=1038),研究了房地产买卖双方的评估模式、评估模式产生的房产属性信息,关注差异及后果,以及卖方观点采择能力的调节作用。研究发现:(1)在房产买卖的决策中,买方处于联合评估模式,卖方处于单独评估模式,前者在决策中更易受房产数量信息属性的影响,后者则易受类别信息属性的影响,进而导致双方就同一房产的评价和估值产生差异。(2)卖方的观点采择能力起到调节作用。本研究的创新之处:将行为决策理论应用于房地产买卖决策领域,丰富和扩展了房地产消费行为理论。在实践层面的应用价值体现在:房地产中介及市场参与者应更关注房产买卖双方决策模式的不同,以及由此产生的对房产属性关注的差异,需要合理反馈到房地产市场营销策划方案中。 展开更多
关键词 房地产买卖 购买决策 行为决策理论 评估模式 不动产偏好
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STEAM教育理念下数学拓展课教学设计研究 被引量:4
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作者 赵千惠 张维忠 《浙江师范大学学报(自然科学版)》 CAS 2024年第2期234-240,共7页
为了达成培育真实情境问题解决能力的目标,围绕以跨学科为核心特征的STEAM教育,寻求数学拓展课的优化路径.从确定以真实情境问题解决能力为目标、设计体现多维化特征的教学内容、实施彰显数学化脉络的教学活动、设定关注学习过程的评价... 为了达成培育真实情境问题解决能力的目标,围绕以跨学科为核心特征的STEAM教育,寻求数学拓展课的优化路径.从确定以真实情境问题解决能力为目标、设计体现多维化特征的教学内容、实施彰显数学化脉络的教学活动、设定关注学习过程的评价侧重点4个维度切入,剖析STEAM教育理念对数学拓展课教学的指导价值,提炼教学基本理念,并构建了STEAM教育理念下的数学拓展课教学基本流程.最后归纳总结出教学设计与实施过程中应注意的关键点:防止过度数学生活化,妥善处理情境虚实;关注协作创新过程,培育工程设计思维;合理融入人文艺术,凸显数学学科本质. 展开更多
关键词 STEAM教育理念 数学拓展课 教学设计 问题解决能力 真实情境
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人与自然和谐共生的中国式现代化--一项基于多维度的历时性考察 被引量:1
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作者 马元喜 方盛举 《学术探索》 CSSCI 2024年第3期1-9,共9页
自中华人民共和国成立以来,中国的现代化建设历经传统、统筹与创新三个阶段,构成了富有中国特色的现代化建设图景。其中,关于自然价值观的更迭,从生产质料转变为保障条件,再到新型财富,其价值得以不断扩容与彰显。同时,随着自然价值观... 自中华人民共和国成立以来,中国的现代化建设历经传统、统筹与创新三个阶段,构成了富有中国特色的现代化建设图景。其中,关于自然价值观的更迭,从生产质料转变为保障条件,再到新型财富,其价值得以不断扩容与彰显。同时,随着自然价值观的变化,人与自然关系也从传统阶段的相对分离,转变为统筹阶段的共在共存,再向创新阶段的共生共荣关系抵近。在这一关系变迁中,从发展优先为起点,到发展与保护统筹兼顾,最终演变为生态优先,实现了经济效益、生态效益与社会效益的统一。纵观整个中国的现代化建设历程,绿色型发展作为通往人与自然和谐共生现代化的路径保障,实现了对传统发展模式的创造性解构,进而为通往人类现代化新形态贡献中国智慧与方案。 展开更多
关键词 中国式现代化 自然价值观 人与自然 发展与保护 绿色型发展
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球茎甘蓝MYB62转录因子的克隆与表达特征分析
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作者 王鑫淼 赵孟良 +2 位作者 邵登魁 尕桑 任延靖 《华北农学报》 CSCD 北大核心 2024年第2期62-70,共9页
为了明晰球茎甘蓝MYB62转录因子的序列特征及其在逆境胁迫后的表达特征,进一步探索球茎甘蓝MYB62转录因子的生物学功能,为后续MYB62转录因子功能鉴定提供理论依据,以球茎甘蓝为研究对象,采用同源克隆的方法获得球茎甘蓝MYB62转录因子基... 为了明晰球茎甘蓝MYB62转录因子的序列特征及其在逆境胁迫后的表达特征,进一步探索球茎甘蓝MYB62转录因子的生物学功能,为后续MYB62转录因子功能鉴定提供理论依据,以球茎甘蓝为研究对象,采用同源克隆的方法获得球茎甘蓝MYB62转录因子基因,并对其进行生物信息学分析,通过实时荧光定量PCR的方法分析MYB62在球茎甘蓝不同组织间以及逆境胁迫后的表达特征。基因克隆结果表明,BocMYB62基因gDNA全长1353 bp,CDS为837 bp,包括4个外显子和3个内含子,编码278个氨基酸。序列结构分析结果显示,BocMYB62为亲水性蛋白,具有2个SANT-MYB结构域,属于R2R3-MYB型MYB转录因子;空间结构预测显示其具有典型的α-螺旋结构;系统发育分析表明,BocMYB62与甘蓝型油菜MYB62亲缘关系最近。时空表达结果显示,BocMYB62在绿色球茎甘蓝中的表达量始终高于在紫色球茎甘蓝中,且存在明显的组织特异性,在干旱胁迫后BocMYB62表达量显著升高,胁迫12 h表达量最高,低温胁迫后BocMYB62表达量显著低于对照,在4℃低温胁迫时表达量最低,推断BocMYB62可能参与花青素生物合成调控,并可能参与逆境胁迫的调控作用。 展开更多
关键词 球茎甘蓝 MYB62 序列分析 保守基序 亲缘关系 荧光定量PCR
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