CHDTEPDB:Transcriptome Expression Profile Database and Interactive Analysis Platform for Congenital Heart Disease

CHDTEPDB(URL:http://chdtepdb.com/)is a manually integrated database for congenital heart disease(CHD)that stores the expression profiling data of CHD derived from published papers,aiming to provide rich resources for investigating a deeper correlation between human CHD and aberrant transcriptome expression.The develop-ment of human diseases involves important regulatory roles of RNAs,and expression profiling data can reflect the underlying etiology of inherited diseases.Hence,collecting and compiling expression profiling data is of critical significance for a comprehensive understanding of the mechanisms and functions that underpin genetic diseases.CHDTEPDB stores the expression profiles of over 200 sets of 7 types of CHD and provides users with more convenient basic analytical functions.Due to the differences in clinical indicators such as disease type and unavoidable detection errors among various datasets,users are able to customize their selection of corresponding data for personalized analysis.Moreover,we provide a submission page for researchers to submit their own data so that increasing expression profiles as well as some other histological data could be supplemented to the database.CHDTEPDB is a user-friendly interface that allows users to quickly browse,retrieve,download,and analyze their target samples.CHDTEPDB will significantly improve the current knowledge of expression profiling data in CHD and has the potential to be exploited as an important tool for future research on the disease.

Correlation between endothelia cells activation and imbalance of cytokines in pulmonary hypertension of congenital heart disease

Objective To explore the correlation between endothelia cells activation and cytokines (ET-1, NO) levels in patients with pulmonary hypertension (PH), and to discuss their roles in the development of PH. Methods Twenty patients with simple ventricular septal defect (VSD) were chosen as controls, and 30 patients with PH were studied. Plasma levels of ET-1 and NO were measured by radioimmunoassay or colorimetric method. Before cardiopulmonary bypass was established, the specimens from right lung were fixed with formaldehyde solution, embedded with paraffin and stained by SP immunohistochemistry. Intercellular adhesion molecule-1 (ICAM-1) expression was measured through the determination of the light density with computer imaging technology. Results Compared with that of the patients with simple VSD, the light density of ICAM-1 and plasma level of ET-1 increased in patients with PH; but plasma level of NO decreased (P<0.05). Positive correlation was observed between ICAM-1 and ET-1/NO (P<0.05). Conclusion Endothelia cells activation and imbalance of ET-1/NO might play an important role in the development of PH.

Efficacy of Danlou Tablet in the Treatment of Coronary Heart Disease with Phlegm and Blood Stasis Syndrome and Its Effects on Serum Inflammatory Factors

[Objectives] To explore the efficacy of Danlou Tablet( DLT) in the treatment of coronary heart disease( CHD) with phlegm and blood stasis syndrome and its effects on serum inflammatory factors. [Methods]One hundred and ninety-seven patients with CHD and phlegm and blood stasis syndrome in our hospital from January 2016 to January 2018 were selected and randomly divided into two groups: control group( n =98) treated with aspirin plus atorvastatin,and research group( n =99) treated with DLT and aspirin plus atorvastatin for one month. The clinical efficacy and incidence of adverse reactions were observed. Serum secretory phospholipase A2( s PLA2),lipoprotein-associated phospholipase A2( LP-PLA2),oxidized low-density lipoprotein( ox-LDL),monocyte chemoattractant protein-1( MCP-1) and World Health Organization Quality of Life( WHOQOL-100) scores were compared before and after one month of treatment. [Results] The total effective rate was93. 94% in the research group,which was higher than that in the control group( 79. 59%,P < 0. 05);the levels of serum s PLA2,LP-PLA2,ox-LDL and MCP-1 in the research group were lower than those in the control group after one month of treatment( P < 0. 05). There was no statistical significance of the difference in the total incidence of adverse reactions between the research group and the control group( P > 0. 05).After one month of treatment,WHOQOL-100 scores were higher in two groups,which were higher in the research group than that in the control group( P < 0. 05). [Conclusions]DLT can significantly reduce the level of serum inflammatory factors,improve the quality of life in patients with CHD and phlegm and blood stasis syndrome.

Effect of Psychological Education Combined with Progressive Muscle Relaxation Training on the Symptom Cluster and Rehospitalization Rate of Elderly Patients with Coronary Heart Disease

Objective:To investigate the effect of psychological education combined with progressive muscle relaxation training on the symptom cluster and rehospitalization of elderly patients with coronary heart disease.Methods:This study is a longitudinal randomized controlled study involving 140 elderly patients with coronary heart disease.The patients were divided into two groups:an intervention group and a control group,with 70 cases in each group,via random number table.The patients in the control group received routine nursing,whereas those in the intervention group received psychological education combined with progressive muscle relaxation training for 4 weeks on the basis of routine nursing.The effect of the intervention was evaluated before intervention,at the end of 1 month,3 months,and 6 months after intervention.Results:At the end of 1 month,3 months,and 6 months,the sleep,fatigue,anxiety,and functional status of the patients in the intervention group were significantly better than those of the control group,with statistical significance difference(P<0.05).The rehospitalization rate of the intervention group was lower than that of the control group,and the difference was statistically significant(X2=10.685,P=0.001).Conclusion:Psychological education combined with progressive muscle relaxation training is effective in alleviating the symptom cluster of elderly patients with coronary heart disease and reducing their rehospitalization rate;thus,it should be popularized.

Maternal pre-conception serum homocysteine instead of folate concentration predict offspring congenital heart diseases:evidence from the real world patient data

Objective This study aimed to investigate predictability of preconception maternal circulating folate and homocysteine concentrations,glycemic and lipid profiles to the risk of congenital heart disease(CHD)in offspring.Methods A cohort of pregnancies was derived from a single hospital where pre-and post-conceptional serum folate,homocysteine,lipids,glycemic profiling and neonatal CHD screening program were facilitated.

Combined effects of hypertension and angiotensin Ⅱ on the risk of coronary heart disease:a population-based prospective cohort study among Inner Mongolians in China

Objective To investigate the combined effects of hypertension and angiotensinⅡon the risk of coronary heart disease(CHD)on the basis of a 10-year prospective study in an Inner Mongolian population of China.Methods Based on a cross-sectional survey,a prospective cohort study was conducted from June 2003 to July 2012 among 2,530 Mongolian people.

Depression as a risk factor for coronary heart disease—How strong is the evidence?

A critical appraisal is made of the evidence that depression is a causal risk factor for coronary heart disease. PubMed and Science Citation Index were searched for relevant papers. Forty eight papers satisfying inclusion criteria and reporting an association between a measure of depression and a coronary disease outcome were compared in terms of baseline assessment, exposure and endpoint definition, covariates measured and whether changes in, or treatment of, depression was assessed during follow-up. There was considerable variation in the definition of depression and coronary heart disease and contradictory findings are reported. Conventional risk factors for coronary heart disease were not assessed consistently or adequately. Only three of the forty-eight papers gave consideration to the time course of depression during follow-up and prior to study entry. Potentially confounding variables such as anxiety, personality traits and other psychiatric disorders were not taken into consideration in the majority of papers. Treatment of depression during the follow-up period was not mentioned in any of the papers. In light of identified methodological shortcomings and the inconsistent findings reported we suggest that there is as yet no convincing evidence that depression is an independent causal risk factor for coronary heart disease.

Research progress on the correlation between TCM syndromes of coronary heart disease and blood biochemical indexes

The researches on the correlation between TCM syndromes of coronary heart disease and blood biochemical indexes from Jan.2011 to Jul.2020 were reviewed,in order to explore the association of different TCM syndromes of coronary heart disease with blood biochemical indexes,such as blood lipid metabolism,inflammatory factors,coagulation-fibrinolysis system,blood rheology and vascular endothelial function,which was hoped to provide a guidance on the clinical combined application of TCM syndrome differentiation and biochemical indexes of coronary heart disease,so as to promote the objectification and standardization of TCM syndrome differentiation.

Clinical perspective on C-reactive protein in prognostication of major adverse cardiac events in the elderly with established coronary heart disease

The systemic response to tissue injury, regardless of cause is characterized by a cytokine-mediated alteration in the hepatic synthesis of a number of different plasma proteins,known collectively as 'acute phase reactants'. These proteins include C-reactive protein, serum amyloid A protein, alphal glycoprotein, ceruloplasmin, alpha macroglobulins, complement components (C1-C4, factor B, C9, C11), alpha1antitrypsin, alpha1 antichymotrypsin, fibrinogen, prothrombin,factor Ⅷ, plasminogen, haptoglobin, ferritin, immunoglobulins and lipoproteins. The initiation of the acute phase response is linked to the production of hormone-like polypeptide mediators now called cytokines, namedly, interleukin 1(IL-1),tumor necrosis factor, interferon gamma, interleukin 6 (IL-6),leukemia inhibitory factor, ciliary neurotropic factor, oncostatin M, and interleukin 11 (IL- 11).……

Study on the relationship between Lp-PLA2, D- dimer and Galectin-3 and atherosclerotic vulnerable plaque in patients with coronary heart disease

Objective: To explore the relationship and clinical value of serum phospholipase A2 (Lp-PLA2), d-dimers, and serum galectin-3 (galectin-3) with atherosclerotic vulnerable plaques in coronary artery patients with coronary heart disease. Methods: A total of 248 patients who underwent coronary angiography (CAG) and intravascular ultrasound (IVUS) in our hospital from June 2017 to September 2018 were selected and divided into vulnerable plaque group (89), stable plaque group (89) and control group (70) according to the examination results. The serum levels of Lp-PLA2, d-dimer and galectin-3 in three groups were compared, as well as their correlation with the detection parameters. To evaluate the clinical value of Lp-PLA2, d-dimer and galectin-3 in patients with coronary heart disease (CHD) with atherosclerotic vulnerable plaque. Results: Serum Lp-PLA2, d-dimer and galectin-3 levels were significantly different from the three groups (P<0.05), and the control group < stable plaque group <vulnerable plaque group (P<0.05). Correlation analysis showed that Lp-PLA2, d-dimer and galectin-3 were significantly positively correlated with plaque area, plaque load, necrotic core and calcified tissue (P<0.01), and negatively correlated with fibrous lipid and fibrous tissue (P<0.01). ROC curve showed that Lp-PLA2, d-dimer and galectin-3 had certain predictive value for vulnerable coronary atherosclerotic plaques (AUC=0.939, 0.977, 0.920, P<0.01), and the three combinations (AUC=0.986, P<0.01) had higher predictive value. Conclusion: Serum Lp-PLA2, d-dimer and galectin-3 are significantly correlated with coronary atherosclerotic vulnerable plaques in patients with coronary heart disease, with high sensitivity and specificity, which can be used for the diagnosis and treatment of early atherosclerotic vulnerable plaques.

Persistence of Dioxin TCDD in Southern Vietnam Soil and Water Environments and Maternal Exposure Pathways with Potential Consequences on Congenital Heart Disease Prevalence in Vietnam

Waterlogged soils and submerged sediments in wetlands and agricultural lands used for rice paddies and aquaculture have anaerobic conditions that slow and prevent the photo and microbial degradation of dioxin TCDD (2,3,7,8-tetracholorodibenzo-p-dioxin), enabling it to persist in environments for long periods. Over 1.6 million ha of land in southern Vietnam were sprayed with 2,4,5-T herbicides (e.g. Agent Orange) contaminated with dioxin TCDD during the Vietnam War (1961-1971);45% of these ha received four or more spray flight missions. Dioxins are endocrine disrupters and may induce cardiovascular disease, growth, and developmental defects, diabetes, hormonal dysfunctions and disruptions, certain cancers, and chloracne. Outpatient screening clinic 2020 data on Vietnamese children suspected of congenital heart disease (CHD) showed the childhood CHD prevalence rate in Vietnam of 13.356/1000, significantly elevated compared to the Asian CHD prevalence rate of 3.531/1000. CHD prevalence rate differences between North Vietnam (2.541/1000) and south of the 17th parallel (10.809/1000) were significant. Vietnamese farmers, especially pregnant women whose occupations involve daily contact with soil and sediments where dioxin TCDD persists in the environment may be at risk of dioxin accumulation from dermal exposure and bioaccumulation via diet. There is an urgent need for funded longitudinal genetic and clinical studies to assess CHD and other organ system childhood malformations due to in utero TCDD exposure. We recommend an integrated research design involving 1) site-specific locations that received high volumes and multiple spray loads of herbicides during the Vietnam War;2) soil sampling of submerged and waterlogged soils and sediments where TCDD may not have degraded;3) production areas of agriculture, fisheries, and other aquatic products;4) risk assessment dioxin levels in foods where TCDD is likely to bioaccumulate;5) child-bearing age and pregnant women with potentially high sensitivity to long-term low dose exposure, and 6) men and women in occupations that are in daily contact with contaminated soil and sediments as part of their job routines.

Systematic Evaluation and Meta-analysis on the Efficacy and Safety of Qidongyixin Oral Liquid(芪冬颐心口服液)in the Treatment of Coronary Heart Disease

Objective:To systematically evaluate the efficacy and safety of Qidongyixin Oral Liquid(芪冬颐心口服液)in the treatment of coronary heart disease.Methods:Randomized controlled trials(RCTs)on the treatment of coronary heart disease(CHD)with Qidongyixin Oral Liquid(芪冬颐心口服液)were screened out by systematically searching in CNKI,Wanfang Data,VIP,Sinomed,Cochrane Library,PubMed,Embase,Web of Science,Clinical-Trials.gov.Meta-analysis of the final included studies was performed according to RevMan 5.3 software.Results:A total of 9 studies were included,with a total sample size of 1224 cases,including 612 in the experimental group and 612 in the control group.Metaanalysis results showed that:Conventional treatment,combined with Qidongyixin Oral Liquid(芪冬颐心口服液)on the treatment of CHD had a significant efficiency,which was better than conventional treatment only(RR=1.18,95%CI[1.07-1.29],P=0.0005),and could better improve patients’electrocardiogram(RR=1.21,95%CI[1.08,1.35],P=0.001),increase left ventricle ejection fraction(MD=3.84,95%CI[3.34,4.33],P<0.00001),and reduce left ventricular end-diastolic diameter(MD=-2.81,95%CI[-3.84,-2.41],P<0.00001)and left ventricular end-systolic diameter(MD=-2.43,95%CI[-3.42,-1.43],P<0.00001).Conclusion:Qidongyixin Oral Liquid(芪冬颐心口服液)can improve the effective rate of the treatment of coronary heart disease,better improve patients’electrocardiogram,reduce the left ventricular end-diastolic and end-systolic diameter,and reduce the onset time of angina pectoris.However,the quality of the cases included in the study is relatively small,and the level of evidence is low.Therefore,RCT with large samples and rigorous design is still needed to prove the reliability of the results.

Risk Assessment and Risk Perception of Coronary Heart Disease in Gaza Strip, Palastine

Aim: The aim of the present study was to estimate the prevalence of coronary heart diseases (CHD) risk factors among Ghaza (Palastine) university students, to assess the CHD risk among them using a suitable scoring system and to identify how they perceive their risk of CHD. Methods: During the period from May 2008 to May 2009, 501 students were involved. Participants were subjected to the following activities;self administered questionnaire: including socio-demographic data, medical history of chronic diseases, family history about CHD, knowledge and perception of risk factors, anthropometric measurements in addition to laboratory testing. Results: The mean age was 20.8 ± 2.07 years;54% were females. The prevalence of hypertension and DM was 3.6% and 0.4% while it was 2.6% for hypercholesterolemia. The mean levels of LDL-C (88 mg/dl vs 85.5 mg/dl) and HDL-C (52.4 mg/dl vs 42.6 mg/dl) were higher among females than among males. Smoking was more prevalent among males than among females (33.1% vs 1.7%) with a total prevalence of 19%. Overweight and obesity were more prevalent among males (30.7% vs 22.5% and 9.6% vs 5.6%). In contrast to risk perception female students tend to be more knowledgeable than males with regards to different aspects of CHD. The overall level of perceived risk was moderate. Logistic regression analysis revealed that age and sex were associated significantly by higher level of total perception (p < 0.05). Conclusion: Health education and health promotion programs should be implemented and integrated within the primary health care sectors and directed to university students before admission. Further research needed to be implemented on larger sample to test knowledge and perception of the public in regard to their risk for heart disease including school children and community so that education could be provided in a more focused manner.

Grid Search for Predicting Coronary Heart Disease by Tuning Hyper-Parameters

Diagnosing the cardiovascular disease is one of the biggest medical difficulties in recent years.Coronary cardiovascular(CHD)is a kind of heart and blood vascular disease.Predicting this sort of cardiac illness leads to more precise decisions for cardiac disorders.Implementing Grid Search Optimization(GSO)machine training models is therefore a useful way to forecast the sickness as soon as possible.The state-of-the-art work is the tuning of the hyperparameter together with the selection of the feature by utilizing the model search to minimize the false-negative rate.Three models with a cross-validation approach do the required task.Feature Selection based on the use of statistical and correlation matrices for multivariate analysis.For Random Search and Grid Search models,extensive comparison findings are produced utilizing retrieval,F1 score,and precision measurements.The models are evaluated using the metrics and kappa statistics that illustrate the three models’comparability.The study effort focuses on optimizing function selection,tweaking hyperparameters to improve model accuracy and the prediction of heart disease by examining Framingham datasets using random forestry classification.Tuning the hyperparameter in the model of grid search thus decreases the erroneous rate achieves global optimization.

Lipid Profile of Coronary Heart Disease Patients: A Prospective Observational Study

Background: Coronary heart disease (CHD) remains the major cause of mortality and morbidity in the entire world population despite therapeutic advances that control many risk factors. Lipid profile is regarded as an important factor in the development of coronary heart disease. There have been numerous studies confirming the association of hyperlipidemias with coronary heart disease in most of the Western as well as Asian countries of the world. But we have very little data on the relation between CHD and lipid profile. Aim of the Study: The aim of this study was to assess abnormal lipid levels and their association with coronary heart disease. Methods: This comparative observational study was conducted at the Department of Cardiology in M. Abdur Rahim Medical College, Dinajpur, Bangladesh from January 2019 to December 2019. Properly written consent was taken from all the participants before starting the main part of the investigation. Obeying inclusion and exclusion criteria of the study, a total of 131 participants were finalized as the study population. Among them, the Case Group was formed with 90 patients who had CHD, and with 41 healthy people, the Control Group was formed. All necessary data were collected, analyzed, and disseminated by several programs of MS-Office and SPSS version 23. Result: The study was conducted with 90 case group participants and 41 control group participants. Among the case group, 78% were male and 22% were female. Among the male case group participants, 44.29% were from 51 - 60 years of age, 35.71% were from 41 - 50 years of age and the rest 20% were above 60 years of age. On the other hand, among the female participants of Case Group, 45% were from 51 - 60 years of age, 30% from 41 - 50 years of age, and 25% were over 60 years of age. Regression analysis between the male and the female showed the P-value of 0.242. Serum cholesterol concentration and HDL concentration were significant between the Case and Control groups in all age groups and the P-value was <0.0001 in every age group. Triglyceride concentration was significant between the Case and Control groups in 41 - 50 and 51 - 60 years age groups with a P-value of <0.0001. But in the >60 years age group, we did not find any significant correlation and there the P-value was 0.478. LDL concentration between the groups showed significant correlations between the Case and Control groups in 41 - 50 and 51 - 60-years’ age groups where the P values were found, <0.0001 and 0.0002 respectively. But in the >60 years’ age group, we did not find any significant correlation and there the P- value was 0.515. Conclusion: In this study, it was found that the total cholesterol, triglycerides, HDL cholesterol, and LDL cholesterol concentrations were significantly higher in coronary heart disease (CHD) patients.

Pulse Wave Analysis in Patients with Coronary Heart Disease Based on Hilbert-Huang Transformation and Time-domain

Objective: To study the characteristics of pulse tracings in CHD, and objectively evaluate the significance of pulse signal in diagnosis and appreciation of therapeutic effect in patients with coronary heart disease(CHD), and accordingly provide with theoretic proofs for developing non-invasive technique of pulse diagnosis. Methods: By using the pulse detection system, pulse graphs in CHD patients, patients without CHD and "health" adults were collected and compared. Then characters of the pulse signal were analyzed with Hilbert-Huang transformation routine (HHT) and time-domain method respectively. Results: There existed characteristic change in pulse graph in CHD. ① h1,h3,h4,h3/h1,t,t5/t4 in time domain parameters of pulse graph increased and w1 was widened. ② 44% of C2 wave in HHT display showed chaotic and disorderly wave and irregularly wave amplitude in CHD. And 72% of C5 Wave exhibited in irregular wave with average wave amplitude over 10 gram-forces. These changes were significantly different from health adults. Conclusion: Characteristic wave of pulse graph extracted with methods of time domain or HHT routine might be considered as proofs for diagnosis and differentiation in CHD. Our researches prognosticate that pulse diagnosis can be used as an ancillary determination in occurrence of CHD for reasons of the advantage of convenient operation and non-invasion.

Coronary heart disease-related fatigue:risk factors,assessment and treatments

Coronary atherosclerotic heart disease is a disease of myocardial ischemia and hypoxia,which often presents as chest pain,dyspnea,cold sweat and fatigue.Fatigue is the subjective experience of patients,which is easy to be ignored,and will lead to the decline of patients’quality of life and physical activity level,etc.,with a high incidence and great harm.The purpose of this paper was to review the concept,risk factors,assessment tools and intervention measures of coronary atherosclerotic heart disease fatigue in order to provide a reference for identifying and improving the fatigue of coronary atherosclerotic heart disease.

Reducing cholesterol to prevent coronary heart disease

Coronary heart disease (CHD) remains the number one killer of men and women in the United States of America despite major advances in interventional technologies for the treatment of coronary artery disease. CHD is rapidly becoming a major cause of morbidity and mortality in developing nations as well and is now recognized as the leading cause of death worldwide.

Metabolic profile of heart tissue in cyanotic conaenital heart disease

Background Cyanotic congenital heart disease(CCHD)is one ofthe most common birth anomaly,in which chronic hypoxia is the basic pathophysiological process.Researches in the protective mechanisms of cardiomyocytes to chronic hypoxia have the potential to provide novel treatment strategies for many patients.Metabolomics is a study of metabolism at global level,which capture global biochemical events by assaying thousands of small molecules in tissues,followed by application of bioinformatics to define metabolomic signatures of the targets.In order to describe the features of metabolic remodeling of the heart of CCHD,we conducted our study on the effect of hypoxia on metabolism of human heart by using tissue from patients with CCHD compared with that from patients with non-cyanotic CHD(NCCHD).