Prognostic value of C-reactive protein levels within 6 hours after the onset of acute anterior myocardial infarction with primary PCI

Background Increased levels of inflammatory markers have been documented in various settings of coronary artery disease. The vulnerability of coronary lesions in acute myocardial infarction(AMI) at the time of onset may be related to serum levels of C reactive protein(CRP) on admission, before CRP levels are affected by myocardial damage.Objective This study assessed the predictive value of CRP levels within six hours after the onset of acute anterior myocardial infarction with primary percutaneous coronary intervention(PCI).Methods The plasma CRP of 76 patients with first acute anterior myocardial infarction was measured within 6 hours after onset. They were divided into 2 groups: group 1( n =20) with elevated CRP( ≥0.3mg/dl ) on admission within 6 hours after onset and group 2( n =56) with normal CRP( <0.3mg/dl ) within 6 hours after onset. All patients were treated by primary PCI. The primary combined end points, including death due to cardiac causes, re MI related to the infarction artery(RIA) and repeat intervention of the RIA, and the restenosis rate were assessed in relation to CRP levels within 6 hours after onset. Left ventricular end diastolic volume index(EDVI),end systolic volume index(ESVI),and ejection fraction(EF) on admission and 6 month after the onset were assessed by left ventriculography. Changes in EDVI(ΔEDVI),ESVI(ΔESVI), and EF(ΔEF) were obtained by subtracting respective on admission values from corresponding 6 month follow up values. Results There were no significant differences in baseline characteristics between the two groups. The primary combined end points were significantly more frequent in group 1(20%) than those in group 2( 1.79% , P <0.01 ).In addition, restenosis rates were significantly higher in group 1 than in group 2(41.18% vs 16.07%, P<0.05). Group 1 showed greater increases in left ventricular volume and less improvement in EF compared with group 2(ΔEDVI 6.31 ±2.17 vs 3.29 ±9.46ml/m 2 , ΔESVI 5.92 ±2.31 vs 3.86 ±1.08ml/m 2 , ΔEF 1.92 ±0.47 vs 4.79 ±1.73% , P <0.05 , respectively).Conclusions CRP levels within 6 hours after the onset of AMI might predict adverse outcome after primary PCI and progressive ventricular remodeling within 6 month of AMI.

Correlation of Serum C-Reactive Protein with Disease Severity in Tuberculosis Patients

Purpose: To study the factors influencing sputum smear conversion including Serum C-Reactive Protein (CRP) and its correlation with disease severity in tuberculosis patients. Method: Levels of Serum-CRP concentrations were deter-mined in 60 patients with pulmonary tuberculosis, 30 healthy volunteers and patients in follow-up after completion of antitubercular treatment (DOTS therapy). Results: Serum-CRP levels were found to be significantly higher in smear-positive group as compared with the follow-up patients and smear-negative control group. The values were 43.65 ± 23.68, 9.88 ± 5.23 and 4.04 ± 3.85 mg/L respectively (P Conclusion: Serum-CRP levels are significantly correlated with disease severity in patients with active pulmonary tuberculosis. Thus these findings from the present study would certainly add new criteria for early diagnosis of TB, which may lead to development of new strategies to treat TB.

Comparative evaluation of acute phase proteins by C-reactive protein(CRP)and serum amyloid A(SAA)in nonhuman primates and feline carnivores

The feasibility of a commercially available assay for C-reactive protein(CRP,CRP for humans:hCRP,and CRP for dogs:vCRP)and a trial reagent of serum amyloid A(SAA,vSAA for animals)were applied to the measurement of acute phase proteins in zoo animals,particularly in nonhuman primates and feline carnivores was evaluate.Results showed that hCRP and vSAA methods were applicable to measure CRP and SAA in Haplorhini.There was a highly signifcant correlation between both parameters with remarkably high correlation coefcient.A higher proportion of Bonnet macaques in Haplorhini,and the linear regression with good correlation between hCRP and vSAA levels were observed.Reference values in healthy Bonnet macaques were hCRP(46.86±30.97 nmol/L)and vSAA(9.06±1.95μg/mL).Although Ring-tailed lemur,which belonging to Strepsirrhini,showed low vSAA concentrations(reference values:1.08±0.47μg/mL),vSAA in patients was apparently elevated.The vCRP and vSAA methods were applicable to measurements of CRP and SAA in feline carnivores for highly signifcant correlation between both parameters.Theses two methods were also been deteded in lions,tigers and cheetahs.vSAA assays can be applied to measure SAA levels in other carnivores and herbivores.In conclusion,vSAA systems have potential utility as diagnostic tools for health screening and prediction in zoo animals.

The Effect of Silica Dust Exposure on the Serum Clara Cell Protein 16 Levels in Chinese Workers

Pneumoconiosis is a systemic occupational disease that commonly manifests as diffuse fibrosis of the lungs caused by long-term inhalation of silica dust in the production process. The development of pneumoconiosis is a chronic and progressive process;once it occurs, it is irreversible, and gradually develops into lung injury and respiratory failure, and can cause death regardless of exposure to dust. The incidence of pneumoconiosis is the highest in China and the direct and indirect economic loss as a result of pneumoconiosis is approximately 9 billion yuan per year.

Study on the correlation and predictive value of serum pregnancyassociated plasma protein A, triglyceride and serum 25-hydroxyvitamin D levels with gestational diabetes mellitus

BACKGROUND Gestational diabetes mellitus(GDM)is a concern due to its rapid increase in incidence in recent years.AIM To investigate the correlation and predictive value of serum pregnancyassociated plasma protein A(PAPP-A),triglyceride(TG),and 25-hydroxyvitamin D[25-(OH)D]with GDM in early pregnancy.METHODS A total of 99 patients in early pregnancy admitted to Peking University International Hospital from November 2015 to September 2017 were included,and underwent a fasting glucose test and oral glucose tolerance test screening at 24-28 wk of pregnancy.Of these cases with GDM,51 were assigned to group A and the remaining 48 cases without GDM were enrolled in group B.Serum PAPP-A,TG and 25-(OH)D in the two groups were compared and their correlation with blood sugar was analyzed.In addition,their diagnostic value in GDM was determined using receiver operating characteristic(ROC)curve analysis.RESULTS Group A had markedly lower serum PAPP-A and 25-(OH)D levels and a significantly higher serum TG level than group B,with statistical significance(P<0.05).Furthermore,Pearson analysis identified that PAPP-A and 25-(OH)D levels were negatively correlated with fasting blood glucose(FBG)levels(r=-0.605,P<0.001),(r=-0.597,P<0.001),while TG and FBG levels were positively correlated(r=0.628,P<0.001).The sensitivity,specificity,area under the curve(AUC)and optimal cut-off value of serum PAPP-A level in the diagnosis of GDM were 72.55%,82.35%,0.861 and 16.340,respectively,while the sensitivity of TG in diagnosing GDM was 86.27%,the specificity was 66.67%,the AUC was 0.813,with an optimal cut-off value of 1.796.The corresponding sensitivity,specificity,AUC and optimal cut-off value of serum 25-(OH)D were 64.71%,70.59%,0.721 and 23.140,respectively.Moreover,multivariate logistic regression analysis revealed that FBG,vascular endothelial growth factor,Flt-1,serum PAPP-A,TG,and 25-(OH)D were related risk factors leading to GDM in patients.CONCLUSION Serum PAPP-A,TG,and 25-(OH)D levels are all correlated with blood glucose changes in GDM,and are independent factors affecting the occurrence of GDM and have certain value in the diagnosis of GDM.

Acrylamide Alters Cytoskeletal Protein Level in Rat Serum

To study biomarker of acrylamide （ACR） induced neuropathy, Wistar rats received 20 or 40 mg/kg of ACR by ip injection and the levels of light neurofilament （NF-L）, middle NF （NF-M）, heavy NF （NF-H）, β-actin, α-tubulin, and β-tubulin proteins in serum were evaluated using both SDS-PAGE and Western blotting. Compared to controls, NF-L and NF-M decreased,

Effects of Crude Protein Level of Concentrate Supplement on Production Performance and Serum Biochemical Parameters in Post-fattening Hainan Yellow Cattle

[Objective]The paper was to investigate the effects of crude protein level of concentrate supplement on production performance and serum biochemical parameters of post-fattening Hainan Yellow Cattle.[Method]Twelve Hainan Yellow Cattles with the same genetic backgrounds and similar initial weight of(168.13±0.55)kg were randomly divided into three treatments.Cattle were fed with concentrate supplement containing different levels of crude protein.The trial lasted 60 d.[Result]The daily gain of 17.56%crude protein group was significantly higher than those of 15.55%and 13.56%crude protein groups(P<0.05),and the gross profit of 17.56%crude protein group was markedly higher than that of 13.56%crude protein group(P<0.05),but the feed gain ratio of 17.56%crude protein group was significantly lower than that of 13.56%crude protein group(P<0.05).Compared with 15.55%and 13.56%crude protein groups,the serum total protein content markedly increased(P<0.05),but the urea nitrogen level significantly decreased(P<0.05).[Conclusion]When crude protein level of concentrate supplement was 17.56%,post-fattening Hainan Yellow Cattle could obtain better production performance.

饲粮蛋白水平和酿酒酵母对秦川肉牛血常规指标和氮磷代谢的影响

【目的】研究不同蛋白水平饲粮及酿酒酵母(SC)对秦川肉牛血液生理生化指标和氮磷代谢的影响。【方法】选择18头14月龄初始体质量为(298±9.78)kg/头、健康的秦川肉牛(母牛)作为试验动物,采用2种蛋白水平(13.23%(A1)和(10.59%(A2))饲粮和3种酿酒酵母添加水平(0(B1),4×10^(8) CFU/kg(B2),8×10^(8) CFU/kg(B3))作为两因素进行饲喂试验。采用全收粪(尿)法测定各处理秦川肉牛粪便和尿液中的氮磷含量,试验结束时于晨饲前采用颈静脉穿刺法采血,测定血常规指标和酶活性,试验期55 d。【结果】①试验期11~55 d,饲粮中添加8×10^(8) CFU/kg SC显著提高了秦川肉牛的采食量(P<0.05),A1B3组秦川肉牛采食量最高,显著高于A1B1组(P<0.05)。②B3组秦川肉牛血清中甘油三酯含量显著低于B1组(P<0.05),A2B3组秦川肉牛血清中总蛋白、白蛋白、球蛋白、免疫球蛋白G和免疫球蛋白M含量均显著高于A2B1组(P<0.05)。③B3组秦川肉牛粗蛋白(CP)和中性洗涤纤维(NDF)的表观消化率较B1组分别显著提高了8.44%和16.90%(P<0.05),A2B3组秦川肉牛的粗蛋白、有机物和酸性洗涤纤维表观消化率最高。④与A2B1组相比,A2B3组秦川肉牛粪氮排放减少了31.28%,尿氮排放减少了19.67%,氮消化率提高了7.66%,氮沉积率提高了62.57%。⑤B2和B3组秦川肉牛的粪磷含量显著低于B1组,A2B3组秦川肉牛磷消化率、磷沉积率相较于A2B1组显著升高了35.96%和45.94%(P<0.05)。【结论】本试验条件下,添加8×10^(8) CFU/kg酿酒酵母能够提高秦川肉牛的采食量、免疫水平和营养成分消化率,减少氮磷排放。在10.59%粗蛋白水平下,饲粮添加8×10^(8) CFU/kg酿酒酵母饲喂秦川肉牛效果最好。

饲粮蛋白质水平对舍饲育肥牦牛生长性能、养分表观消化率及血清生化指标的影响

试验旨在探究不同粗蛋白水平饲粮对冷季舍饲集中育肥牦牛生长性能、养分表观消化率及血清生化指标的影响。试验选取体况良好、体重(180.00±11.67)kg的公牦牛30头,随机分为3组,每组10个重复,每个重复1头牦牛。设计3个粗蛋白水平分别为12.5%(Ⅰ组)、15.5%(Ⅱ组)和18.5%(Ⅲ组)的全混合饲粮(TMR)。预试期30 d,正式试验期90 d。结果显示,Ⅰ组、Ⅱ组牦牛的总增重、平均日增重显著高于Ⅲ组(P<0.05);Ⅲ组牦牛的料重比显著高于Ⅰ组、Ⅱ组(P<0.05)。Ⅱ组、Ⅲ组牦牛的粗灰分表观消化率显著高于Ⅰ组(P<0.05)。Ⅲ组牦牛血清中谷丙转氨酶(ALT)活性显著高于Ⅱ组(P<0.05);Ⅲ组尿素氮(BUN)含量显著高于Ⅰ组、Ⅱ组(P<0.05)。研究表明,中蛋白质水平饲粮在牦牛育肥过程所呈现的育肥效果较好,冷季集中育肥牦牛推荐饲粮蛋白质水平为15.5%。

饲粮蛋白质水平对藏羊生长性能、血清生化指标及肉品质的影响

试验旨在探究不同蛋白质水平饲粮对藏羊生长性能、血清生化指标和肉品质的影响。试验选用初始体重相近[(15.40±0.81)kg]且体况良好的2月龄高原型藏羊公羔90只,随机分为3个组,每组5个重复。各组分别饲喂蛋白质水平为12%(A组)、14%(B组)和16%(C组)的饲粮。整个试验分预试期7 d和正试期90 d。结果显示:①各组间的平均日增重、平均日采食量及料重比均差异不显著(P>0.05)。②A组的血清谷丙转氨酶活性高于B组(P>0.05)和C组(P<0.05),B组的血清葡萄糖含量显著低于其他两组(P<0.05),A组的血清总蛋白含量低于B组(P<0.05)和C组(P>0.05)。③C组的肌肉中糖原含量显著高于A组(P<0.05),C组的肌肉中苹果酸脱氢酶活性显著高于其他两组(P<0.05)。④C组的肌纤维直径显著高于B组(P<0.05),B组和C组的肌纤维密度显著高于A组(P<0.05),B组的肌纤维横截面积显著高于C组(P<0.05)。综上,饲粮蛋白水平为16%时,有利于增强藏羊的生长性能。在饲粮蛋白水平为14%时,有利于增强育肥期藏羊肌肉品质。

不同乳产量、乳蛋白率双峰驼血液生化指标和激素水平的对比研究

为了研究不同乳产量和乳蛋白率泌乳双峰驼血液生化指标和激素含量的差异,选用24峰体况、胎次相近的处于泌乳初期的阿拉善双峰驼,分为高产乳量组、低产乳量组、高乳蛋白组、低乳蛋白组,测定其血液指标,包括血清生化指标和血清激素水平。结果显示,在乳产量分组中,高产组双峰驼钙离子和钾离子显著低于低产组(P<0.05),非酯化脂肪酸极显著低于低产组(P<0.01),血清胰岛素浓度显著高于低产组(P<0.05);在乳蛋白率分组中,高乳蛋白组的血尿素氮浓度极显著高于低乳蛋白组(P<0.01),高乳蛋白组的孕酮和雌三醇显著低于低乳蛋白组(P<0.05),催乳素、雌酮和瘦素极显著低于低乳蛋白组(P<0.01)。结果表明,在相同的饲养管理下,离子代谢和糖代谢对乳产量的差异起到重要作用,乳蛋白的合成效率的差异可能主要由激素调节。

The effects of enhanced external counterpulsation on the serum level of C-reactive protein and endothelin-1 in patients with ischemic stroke

Objective To explore the effects of enhanced external counterpulsation(EECP)on the serum level of C-reactive protein and endothelin-1 in patients with cerebral ischemic stroke,to provide clinical evidence for the treatment and secondary prevention of patients with serebral ischemic stroke.Methods Total 187 patients with ischemic stroke and enrolled measure the serum level

布美他尼联合阿魏酸哌嗪对慢性肾小球肾炎患者临床指标的影响

目的 探究布美他尼联合阿魏酸哌嗪对慢性肾小球肾炎(CGN)患者临床指标的影响。方法 选取2020年6月至2022年6月南充市中心医院CGN患者120例作为研究对象,采用随机数字表法将患者分为试验组(采用布美他尼+阿魏酸哌嗪治疗)和对照组(采用阿魏酸哌嗪治疗),每组60例。比较两组患者的临床疗效、炎症指标、肾纤维化和肾功能指标、24 h尿蛋白水平和不良反应发生率。结果 试验组临床疗效(96.67%)明显高于对照组(83.33%)(P<0.05);与治疗前比较,治疗后两组白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、凝血酶激活纤溶抑制物(TAFI)均有所降低,与对照组比较,试验组治疗后IL-6、TNF-α、TAFI水平更低(P<0.05);与治疗前比较,治疗后两组纤维连接蛋白(FN)、转化生长因子β1(TGF-β1)、金属蛋白酶组织抑制因子1(TIMP-1)等肾纤维化指标和血尿素氮(BUN)、血清肌酐(SCR)等肾功能指标均下降,与对照组比较,试验组治疗后肾纤维化、肾功能指标更低(P<0.05);两组治疗后红细胞、白细胞计数、24 h尿蛋白均下降,且试验组尿常规相关指标水平更低(P<0.05);两组不良反应发生率比较差异无统计学意义(P>0.05)。结论 布美他尼联合阿魏酸哌嗪能有效降低CGN患者的尿蛋白、血清TAFI水平和肾纤维化水平,改善肾脏功能。

黄葵胶囊联合缬沙坦对肾小球肾炎的疗效分析

目的探讨黄葵胶囊联合缬沙坦治疗肾小球肾炎(GN)的临床疗效。方法选取2020年6月至2022年2月商丘市中医院收治的GN患者共计94例,根据随机数字表法分成研究组(n=47)与对照组(n=47),对照组采用缬沙坦治疗,研究组采用黄葵胶囊联合缬沙坦治疗,对两组临床疗效、24 h尿蛋白水平、血清细胞因子水平及不良反应进行比较。结果研究组治疗有效率(93.62%)高于对照组(74.47%),差异有统计学意义(P<0.05);两组治疗后24 h尿蛋白排泄率(AER)、24 h尿蛋白量水平均降低,差异有统计学意义(P<0.05),研究组较对照组更低,差异有统计学意义(P<0.05);两组治疗后血清C反应蛋白(CRP)、转化生长因子-β(TGF-β1)、血尿素氮(BUN)、血肌酐(Scr)水平均降低,差异有统计学意义(P<0.05),且研究组较对照组更低,差异有统计学意义(P<0.05);研究组不良反应发生率(6.38%)低于对照组(21.28%),差异有统计学意义(P<0.05)。结论采用黄葵胶囊联合缬沙坦治疗GN患者,可提高治疗效率,改善蛋白水平及血清细胞因子水平,减少不良反应发生。

独活寄生汤联合BKA治疗中老年重度双间室膝关节骨关节炎的效果

目的:探讨独活寄生汤应用于双间室膝关节置换术(bicompartment knee arthroplasty,BKA)治疗中老年患者重度双间室膝关节骨关节炎的效果。方法:选取2020年1月—2023年1月在普定县中医医院诊断为重度双间室膝关节骨关节炎并接受BKA治疗的患者80例,按照随机数字表法分为对照组(40例)和观察组(40例)。对照组给予口服盐酸氨基葡萄糖治疗,观察组在对照组的基础上给予独活寄生汤治疗。比较两组C反应蛋白水平、红细胞沉降率、匹兹堡睡眠质量指数(PSQI)评分。结果:治疗2个月后,观察组总有效率为97.50%,明显高于对照组(82.50%);治疗2个月后,观察组红细胞沉降速率、血清C反应蛋白水平均显著下降,炎症反应水平均明显低于对照组,差异均有统计学意义(P<0.05)。治疗1、2个月后,观察组PSQI评分均明显低于对照组,差异均有统计学意义(P<0.05)。结论:独活寄生汤能够降低重度双间室膝关节骨关节炎患者的炎症反应水平,提高治疗效果,缓解疼痛,提高患者睡眠质量。

Infliximab trough level combined with inflammatory biomarkers predict long-term endoscopic outcomes in Crohn’s disease under infliximab therapy

BACKGROUND Infliximab trough level(ITL)severely affects therapeutic outcomes of Crohn’s disease(CD)patients under infliximab(IFX).Recently,frontier research has focused on identifying ITL based on different therapeutic targets.Although previous studies have elaborated clinical value of ITL monitoring on short-term outcomes in CD patients during therapy,studies contraposing the predictive value of ITL on long-term endoscopic outcomes in CD patients are still scarce domestically and overseas.AIM To explore the predictive value of ITL in combination with inflammatory biomarkers on long-term endoscopic outcomes in CD with clinical remission during IFX maintenance therapy.METHODS CD patients with endoscopic remission under long-term IFX maintenance therapy in the First Affiliated Hospital of Zhejiang Chinese Medicine University from January 2012 to December 2020 were collected.ITL and inflammatory biomarkers were continuously monitored during the therapy.The Step I study was conducted from weeks 14 to 54 of IFX treatment.The Step II study was conducted from weeks 54 to 108 of IFX treatment.Endoscopic outcomes were defined as endoscopic activity(Crohn’s disease endoscopic index of severity score>2 points or Rutgeerts score>i1)and endoscopic remission(Crohn’s disease endoscopic index of severity score≤2 points or Rutgeerts≤i1).Endoscopic relapse free survival was defined as endoscopic remission at the beginning of the study stage and maintaining endoscopic remission during the study stage.RESULTS At week 14,low ITL[odds ratio(OR)=0.666,95%confidence interval(CI):0.514-0.862,P<0.01]and high fecal calprotectin(FCP)level(OR=1.002,95%CI:1.001-1.004,P<0.01)increased the risk of endoscopic activity at week 54.At week 54,low ITL(OR=0.466,95%CI:0.247-0.877,P<0.01)and high C-reactive protein(CRP)level(OR=1.590,95%CI:1.007-2.510,P<0.01)increased the risk of endoscopic activity at week 108.At week 14,ITL≤5.60μg/mL[area under the curve(AUC)=0.83,95%CI:0.73-0.90,P<0.001]and FCP>238μg/g(AUC=0.82,95%CI:0.72-0.89,P<0.001)moderately predicted endoscopic activity at week 54.ITL≤5.60μg/mL in combination with FCP>238μg/g indicated 82.0%possibility of endoscopic activity.At week 54,ITL≤2.10μg/mL(AUC=0.85,95%CI:0.72-0.93,P<0.001)and CRP>3.00 mg/L(AUC=0.73,95%CI:0.60-0.84,P=0.012)moderately predicted moderate endoscopic activity at week 108.ITL≤2.10μg/mL in combination with CRP>3.00 mg/L indicated 100.0%possibility of endoscopic activity.From weeks 14 to 54 of IFX treatment,patients with ITL>5.60μg/mL had higher rate of endoscopic relapse free survival than those with ITL≤5.60μg/mL(95.83%vs 46.67%).From weeks 54 to 108 of IFX treatment,patients with ITL>2.10μg/mL had higher rate of endoscopic survival free relapsed rate than those with ITL≤2.10μg/mL(92.68%vs 30.77%).CONCLUSION Combination of ITL,CRP,and FCP contribute to long-term endoscopic prognosis monitoring.During IFX maintenance treatment,low ITL,high CRP level,and high FCP level were independent risk factors of CD patients with clinical remission in adverse endoscopy outcomes within 1-year follow-up.

Interleukins and Acute Phase Proteins of Bovine Sera during Natural Helminth Burden in Ibadan Nigeria

Inflammatory reactions in the gastrointestinal tract play an important role in the pathogenesis of gastroenteritis in bovine helminthosis. This study determined the serum concentrations of cytokines induced acute phase proteins (Serum Amyloid A (SAA), Haptoglobin (Hp) and C-reactive protein (CRP)) and the levels of immunoreactive interleukins (IL-1, IL-2, IL-3, IL-4, IL-5 and IL-6) in cattle naturally infected with helminths. We sampled a total of 480 slaughtered cattle of both sexes in a major abattoir in Ibadan, Nigeria. Sedimentation, floatation and modified McMaster techniques were employed to determine the degrees of helminth infections. Animals with eggs per gram (epg) less than 200 were adjudged to be apparently healthy. The serum concentrations of interleukins and acute phase proteins were determined using the Technicon AutoAnalyzer Model AA II. It was found that mean SAA (μg/l) and CRP (%) levels were significantly (P 0.05). Therefore, some acute phase proteins are involved in the pathophysiology of bovine helminthosis and are closely related to the inflammatory activation of the disease. In lieu of these findings, it is suggested that systemic markers of inflammation can identify subjects at high risk of natural bovine helminthosis and that IL-6 and SAA may be used as indicators for bovine helminthosis.

Changes of serum CRP, serum uric acid and immunoglobulin in patients with renal damage of systemic lupus erythematosus

Objective: To observe the expression level of serum CRP, serum uric acid and immunoglobulin (IgG, IgA and IgM) in patients with renal damage of systemic lupus erythematosus, and explore their clinical application value. Methods: A total of 95 patients with SLE were divided into lupus nephritis group (LN group, n = 64) and non-lupus nephritis group (NLN group n=31) according to the diagnostic criteria of lupus nephritis, and another 35 healthy subjects were selected as normal control group. Blood samples were collected from all subjects before treatment. Serum CRP and serum uric acid concentrations were measured by ELISA. The levels of serum immunoglobulins were detected by immunoturbidimetry in the same time. Finally, the results were analyzed statistically. Results: The levels of serum CRP and serum uric acid in patients with SLE were significantly higher than those in control group. The levels of serum CRP and serum uric acid in LN group of SLE patients were significantly higher than those in NLN group and control group. Serum CRP level in NLN group was significantly higher than that in control group. Serum uric acid level in NLN group was slightly higher than that of the control group, but the difference was not statistically significant. The level of serum immunoglobulin in SLE patients was higher than that in control group. The levels of serum immunoglobulin IgG, IgA and IgM in LN group of SLE patients were significantly higher than those in NLN group and control group. The levels of serum immunoglobulin IgG, IgA and IgM in NLN group of SLE patients were all higher than those in control group. Conclusion: Humoral immune of SLE patients with renal injury was activated at different degree, B-cell hyperthyroidism, severe systemic inflammatory response and kidney damage at varying degrees. Serum CRP, serum uric acid and immunoglobulin (IgG, IgA and IgM) may be involved in SLE renal injury pathological process, which is the early diagnosis of reference indicators.

Salivary cytokines and levels in denture stomatitis: An exploratory case-control study

We aimed to evaluate the microbial and inflammatory characteristics associated with Denture Stomatitis (DS) analyzing: l) Levels of salivary cytokines and cultivable C. albicans;2) DNA-DNA checkerboard on biofilm associated with mucosal tissue-bearing denture surfaces, 3) Serum C-reactive protein (CRP) levels. Thirty-two subjects were enrolled in the study with control (n = 17) and DS types II and III (n = 15) subjects. Samples were collected from unstimulated whole saliva, serum and swabs from denture surfaces. Salivary levels of inflammatory mediators and CRP were measured by multiplex. Samples from denture and mucosal surfaces were analyzed by DNA-DNA checkerboard. Saliva from DS subjects showed increase in IL-8 (p = 0.04) and IL-1β (p = 0.04) with trend for increase in IL-1β, TNFα and IL-6 levels. C. albicans higher counts in DS saliva (p = 0.03) showed association with elevated levels of IL-8 (p = 0.03) and IL-1α (p = 0.01). CRP levels were not different among groups (p = 0.74). DNA-DNA checkerboard analyses indicated typical periodontal pathogens below the detection threshold of 104 organisms on both denture and inflamed mucosal surfaces. The data suggest that DS is associated with elevation of salivary IL1 and IL-8 together with increased C. albicans. There was no evidence of systemic inflammation as measured by serum C-reactive protein levels.