Objective:To observe the effect of captopril on the tumor necrosis factor-α(TNF- α) level and arterial blood gases in acute lung injury(All) induced by HCL in rats,and to analyze its protective mechanism.Methods:Fif...Objective:To observe the effect of captopril on the tumor necrosis factor-α(TNF- α) level and arterial blood gases in acute lung injury(All) induced by HCL in rats,and to analyze its protective mechanism.Methods:Fifty Wistar rats were selected and randomly divided into three groups,with 20 rats in Group Ⅰ and Ⅱ,respectively and 10 animals in Group Ⅲ.ALI model was constructed by intratracheal injection of diluted hydrochloric acid(pH=1.25.1.2 mL/kg).Group Ⅰrats received not any treatment after construction of AM model.Group Ⅱ rats were treated with captopril(5 mg/kg,i.p.) 5 min after induction of ALI.Group Ⅲ served as normal control without any treatment.Ninety minutes after construction of ALI model,all the rats were sacrificed.Blood was withdrawn for detection of TNF- α level and arterial blood gases index.And lung tissue slices of the three groups were prepared for observation of pathologic histology changes.Results:TNF- α level in serum of Group Ⅰ and Ⅱ rats was significantly higher than that in Group Ⅲ(P<0.05),while TNF- α level in serum of Group Ⅱ was significantly lower in Group Ⅰ(P<0.05).PaCO_2 level was significantly higher(P<0.05),while PaO_2 was significantly lower(P<0.05) in Group Ⅰ and Ⅱ rats than those in Group Ⅲ.PaCO_2 was significantly lower(P<0.05) and PaO_2 was significantly higher(P<0.05) in Group Ⅱ than those in Group Ⅰ.Histological observation showed diffuse congestion and severe edema of luug tissue,obvious thickening and structure damage of alveolar walls and a large amount of neutrophil infiltration in Group Ⅰ rats.Group Ⅱ rats showed mild edema of lung tissue;only a small portion of alveolar walls showed thickening and only a few of neutrophil infiltration could be observed.The degree of injury was remarkably slighter than that of Group Ⅰ rats.Group Ⅲ rats showed clear lung tissue structure and normal morphology:alveolar walls were uniform and the margin was smooth and few neutrophil could be observed.Conclusions:Captopril can significantly reduce serum TNF- α level,elevate PaO_2 and reduce PaCO_2 in rats with ALI.It has a protective effect on ALI rats.展开更多
AIM: To approach the relationship between alveolar echinococcosis (AE) pathology and level of sIL-2R,TNF-α and IFN-γ in sera and the significance of cytokines in development of AE.METHODS: After 23 patients with AE ...AIM: To approach the relationship between alveolar echinococcosis (AE) pathology and level of sIL-2R,TNF-α and IFN-γ in sera and the significance of cytokines in development of AE.METHODS: After 23 patients with AE were confirmed by ELISA and ultrasound, their sera were collected and the concentrations of sIL-2R,TNF-α and IFN-γ were detected by double antibody sandwich. Twelve healthy adults served as controls. According to the status of livers of AE patients by ultrasound scanning, they were divided into 4 groups: P2,P3, P4 groups and C group (control). Average of concentrations of sIL-2R, TNF-α and IFN-yin homologous group was statistically analyzed by both ANOV and Newman-Keuls, respectively.RESULTS: The mean of sIL-2R in P2 group was 97±29, P3:226±80, P4:194±23 and control group (111±30)×10^3 u/L(P<0.01). The mean of TNF-α in P2 group was 1.12±0.20, P3:3.67±1.96, P4:1.30±0.25 and control group 0.40±0.19 μg/L(P<0.01). The mean of IFN-γ in P2 group was 360±20, P3:486±15, P4:259±19 and control group: 16±2 ng/L (P<0.01).Judged by ANOV and Newman-Keuls, the mean concentrations of sIL-2R, TNF-α and IFN-γ had a significant difference among groups. Except for P2group, the mean sIL-2R between other groups of AE patients had a significant difference (P<0.05). The mean of TNF-α concentration in P3 group was the highest (P<0.01). The mean of IFN-γ concentration in all patients was higher than that in control group (P<0.01),but there was no difference between AE groups (P>0.05).CONCLUSION: Low sIL-2R level indicates an early stage of AE or stable status, per contra, a progression stage. Higher level of TNF-α might be related to the lesion of liver. The role of single IFN-γ is limited in immunological defense against AE and it can not fully block pathological progression.展开更多
To identify the roles of serum IL-18, IL- 10, TNF-α and sIL-2R in the pathogenisis of chronic hepatitis C and the effects of interferon on the men- tioned serum cytokines. Methods: The levels of IL-18, IL-10, TNF-α ...To identify the roles of serum IL-18, IL- 10, TNF-α and sIL-2R in the pathogenisis of chronic hepatitis C and the effects of interferon on the men- tioned serum cytokines. Methods: The levels of IL-18, IL-10, TNF-α and sIL-2R were detected in 10 healthy controls, 24 a- symptomatic HCV carriers, and 27 patients with chronic hepatitis C (before and after IFN treatment) by enzyme linked immunosorbent assay (ELISA). Results: The levels of IL-18, IL-10, TNF-α and sIL- 2R in the patients of chronic hepatitis C were higher than those in the healthy controls (P<0.05) and in asymptomatic HCV carriers (P<0. 05). The values of the mentioned cytokines showed a significant posi- tive correlation to GPT. The levels of the mentioned cytokines decreased obviously after IFN treatment (P <0. 05), while the serum levels of IL-10 and sIL-2R reduced in sequence in no-response group, partial- response group and complete-response group. Conclusions: IL-18, IL-10, TNF-α and sIL-2R co- participate in the pathogenisis of chronic hepatitis C, and are used to evaluate the effect of IFN on the im- mune state of organisms, and IL-10 and sIL-2R are important for predicting the anti-viral efficacy of IFN.展开更多
Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control...Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control,sham operation, model and EA groups, and each of them were further divided into IR 12 hr, IR 24 hr and IR 48 hr subgroups, with 10 cases being in each subgroup. Cerebral ischemia was produced by occlusion of the middle cerebral artery for 0.5 hr. Shuigou (GV 26) and Baihui (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus. Serum IL 1β and tumor necrosis factor (TNF) α contents were assayed by using radioimmunoassay. Results: Results showed that serum IL 1β and TNF α contents of model group were significantly higher than those of normal control and sham operation groups at IR 12 hr, 24 hr and 48 hr (P<0.05), meaning that following IR, the production and release of these two cytokines in the blood increased obviously; comparison between EA and model groups displayed that serum IL 1β and TNF α levels of EA group were strikingly lower than those of model group (P<0.01), suggesting that EA stimulation can suppress IR induced increase of production and release of these two cytokines. Conclusion: EA can suppress cerebral IR induced increase of serum IL 1β and TNF α, which may contribute to the effect of acupuncture in resisting cerebral IR injury in the treatment of stroke.展开更多
基金supported by Science and Technology Research and Development Program of Tangshan City,Reference No.07130233d
文摘Objective:To observe the effect of captopril on the tumor necrosis factor-α(TNF- α) level and arterial blood gases in acute lung injury(All) induced by HCL in rats,and to analyze its protective mechanism.Methods:Fifty Wistar rats were selected and randomly divided into three groups,with 20 rats in Group Ⅰ and Ⅱ,respectively and 10 animals in Group Ⅲ.ALI model was constructed by intratracheal injection of diluted hydrochloric acid(pH=1.25.1.2 mL/kg).Group Ⅰrats received not any treatment after construction of AM model.Group Ⅱ rats were treated with captopril(5 mg/kg,i.p.) 5 min after induction of ALI.Group Ⅲ served as normal control without any treatment.Ninety minutes after construction of ALI model,all the rats were sacrificed.Blood was withdrawn for detection of TNF- α level and arterial blood gases index.And lung tissue slices of the three groups were prepared for observation of pathologic histology changes.Results:TNF- α level in serum of Group Ⅰ and Ⅱ rats was significantly higher than that in Group Ⅲ(P<0.05),while TNF- α level in serum of Group Ⅱ was significantly lower in Group Ⅰ(P<0.05).PaCO_2 level was significantly higher(P<0.05),while PaO_2 was significantly lower(P<0.05) in Group Ⅰ and Ⅱ rats than those in Group Ⅲ.PaCO_2 was significantly lower(P<0.05) and PaO_2 was significantly higher(P<0.05) in Group Ⅱ than those in Group Ⅰ.Histological observation showed diffuse congestion and severe edema of luug tissue,obvious thickening and structure damage of alveolar walls and a large amount of neutrophil infiltration in Group Ⅰ rats.Group Ⅱ rats showed mild edema of lung tissue;only a small portion of alveolar walls showed thickening and only a few of neutrophil infiltration could be observed.The degree of injury was remarkably slighter than that of Group Ⅰ rats.Group Ⅲ rats showed clear lung tissue structure and normal morphology:alveolar walls were uniform and the margin was smooth and few neutrophil could be observed.Conclusions:Captopril can significantly reduce serum TNF- α level,elevate PaO_2 and reduce PaCO_2 in rats with ALI.It has a protective effect on ALI rats.
基金Supported by the STD3 Programme of the EC,No.TS3-CT94-0270
文摘AIM: To approach the relationship between alveolar echinococcosis (AE) pathology and level of sIL-2R,TNF-α and IFN-γ in sera and the significance of cytokines in development of AE.METHODS: After 23 patients with AE were confirmed by ELISA and ultrasound, their sera were collected and the concentrations of sIL-2R,TNF-α and IFN-γ were detected by double antibody sandwich. Twelve healthy adults served as controls. According to the status of livers of AE patients by ultrasound scanning, they were divided into 4 groups: P2,P3, P4 groups and C group (control). Average of concentrations of sIL-2R, TNF-α and IFN-yin homologous group was statistically analyzed by both ANOV and Newman-Keuls, respectively.RESULTS: The mean of sIL-2R in P2 group was 97±29, P3:226±80, P4:194±23 and control group (111±30)×10^3 u/L(P<0.01). The mean of TNF-α in P2 group was 1.12±0.20, P3:3.67±1.96, P4:1.30±0.25 and control group 0.40±0.19 μg/L(P<0.01). The mean of IFN-γ in P2 group was 360±20, P3:486±15, P4:259±19 and control group: 16±2 ng/L (P<0.01).Judged by ANOV and Newman-Keuls, the mean concentrations of sIL-2R, TNF-α and IFN-γ had a significant difference among groups. Except for P2group, the mean sIL-2R between other groups of AE patients had a significant difference (P<0.05). The mean of TNF-α concentration in P3 group was the highest (P<0.01). The mean of IFN-γ concentration in all patients was higher than that in control group (P<0.01),but there was no difference between AE groups (P>0.05).CONCLUSION: Low sIL-2R level indicates an early stage of AE or stable status, per contra, a progression stage. Higher level of TNF-α might be related to the lesion of liver. The role of single IFN-γ is limited in immunological defense against AE and it can not fully block pathological progression.
文摘To identify the roles of serum IL-18, IL- 10, TNF-α and sIL-2R in the pathogenisis of chronic hepatitis C and the effects of interferon on the men- tioned serum cytokines. Methods: The levels of IL-18, IL-10, TNF-α and sIL-2R were detected in 10 healthy controls, 24 a- symptomatic HCV carriers, and 27 patients with chronic hepatitis C (before and after IFN treatment) by enzyme linked immunosorbent assay (ELISA). Results: The levels of IL-18, IL-10, TNF-α and sIL- 2R in the patients of chronic hepatitis C were higher than those in the healthy controls (P<0.05) and in asymptomatic HCV carriers (P<0. 05). The values of the mentioned cytokines showed a significant posi- tive correlation to GPT. The levels of the mentioned cytokines decreased obviously after IFN treatment (P <0. 05), while the serum levels of IL-10 and sIL-2R reduced in sequence in no-response group, partial- response group and complete-response group. Conclusions: IL-18, IL-10, TNF-α and sIL-2R co- participate in the pathogenisis of chronic hepatitis C, and are used to evaluate the effect of IFN on the im- mune state of organisms, and IL-10 and sIL-2R are important for predicting the anti-viral efficacy of IFN.
文摘Purpose: To study the mechanism of early acupuncture treatment in resisting inflammatory injury in cerebral ischemia reperfusion (IR) rats. Methods: A total of 120 Wistar rats were randomly divided into normal control,sham operation, model and EA groups, and each of them were further divided into IR 12 hr, IR 24 hr and IR 48 hr subgroups, with 10 cases being in each subgroup. Cerebral ischemia was produced by occlusion of the middle cerebral artery for 0.5 hr. Shuigou (GV 26) and Baihui (GV 20) were punctured and stimulated electrically with an EA therapeutic apparatus. Serum IL 1β and tumor necrosis factor (TNF) α contents were assayed by using radioimmunoassay. Results: Results showed that serum IL 1β and TNF α contents of model group were significantly higher than those of normal control and sham operation groups at IR 12 hr, 24 hr and 48 hr (P<0.05), meaning that following IR, the production and release of these two cytokines in the blood increased obviously; comparison between EA and model groups displayed that serum IL 1β and TNF α levels of EA group were strikingly lower than those of model group (P<0.01), suggesting that EA stimulation can suppress IR induced increase of production and release of these two cytokines. Conclusion: EA can suppress cerebral IR induced increase of serum IL 1β and TNF α, which may contribute to the effect of acupuncture in resisting cerebral IR injury in the treatment of stroke.
