Abstract： Somatic embryogenesis from lily bulb scales has not been studied in details, although tissue culture methods have been applied to the propagation for decades. The effects of different kinds and concentratio...Abstract： Somatic embryogenesis from lily bulb scales has not been studied in details, although tissue culture methods have been applied to the propagation for decades. The effects of different kinds and concentration of auxins for oriental lily somatic embryogenesis were investigated （Lilium hybrida var. Sorbonne）. 2, 4-dichlorophenoxyacetic acid （2, 4-D）, thidiazuron （TDZ） and α-naphthaleneacetic acid （NAA） media with benzyladenine（6-BA） and lactalbumin hydrolysate （LH） were used for embryogenic callus in the darkness. The best response on embryogenic callus formation was obtained on MS media supplemented 2, 4-D 2.0 mg·L^-1, 6-BA 0.5 mg·L^-1 and LH 300 mg·L^-1. Transfer embryogenic callus to the media with TDZ, 6-BA, kinetin （KT） supplemented 2, 4-D. The highest number of somatic embryos has been produced on medium with 0.5 mg·L^-1 2, 4-D and 0.3 mg·L^-1 KT. Germinated embryos with shoot axes were changed to MS media with 6-BA 0.5 mg·L^-1. The results suggest that in vitro culture of somatic embryogenesis from lily bulb scales can be used for plant regeneration.展开更多
Objective:The aim of the study is to investigate the relationship between antenatal depression and adverse perinatal outcomes.Methods:This prospective cohort study enrolled pregnant women between gestational ages of 1...Objective:The aim of the study is to investigate the relationship between antenatal depression and adverse perinatal outcomes.Methods:This prospective cohort study enrolled pregnant women between gestational ages of 12-20 weeks to complete the Chinese version of the Zung Self-Rating Depression Scale(SDS)and followed them for delivery from September 2015 to September 2016.Participants were classified into mild,moderate,and severe depression groups according to the SDS scores.Logistic regression was performed to assess the association between antenatal depression and perinatal outcomes including preterm birth(PTB),cesarean section use,hypertension disorders,gestational diabetes,and thyroid diseases during pregnancy.An age-stratified analysis was performed.Results:A total of 4,663 pregnant women were analyzed.As a result,13.8%,1.7%,and 0.2%of women were classified as mild,moderate,and severe depression,respectively.Severely depressed mothers were at higher risk for PTB(adjusted odds ratio[OR]=11.31,95%confidence interval[CI]2.13-60.03),especially spontaneous PTB.Moderate-depressed women were at higher risk for hyperthyroidism during pregnancy(adjusted OR=3.67,95%CI 1.10-12.27),while women with mild depression tended to choose cesarean sections(OR=1.24,95%CI=1.04-1.49).Age-stratified analysis indicated an elevated risk of adverse outcomes associated with depression in women aged<25 years,but the association was not significant.Conclusions:Antenatal depression was associated with PTB,hyperthyroidism,and cesarean use.Studies with large sample sizes should verify the relationship between PTB and antenatal depression to avoid casual events.展开更多
BACKGROUND Acute pancreatitis(AP),as a common acute abdomen disease,has a high incidence rate worldwide and is often accompanied by severe complications.Negative emotions lead to increased secretion of stress hormones...BACKGROUND Acute pancreatitis(AP),as a common acute abdomen disease,has a high incidence rate worldwide and is often accompanied by severe complications.Negative emotions lead to increased secretion of stress hormones,elevated blood sugar levels,and enhanced insulin resistance,which in turn increases the risk of AP and significantly affects the patient's quality of life.Therefore,exploring the intervention effects of narrative nursing programs on the negative emotions of patients with AP is not only helpful in alleviating psychological stress and improving quality of life but also has significant implications for improving disease outcomes and prognosis.AIM To construct a narrative nursing model for negative emotions in patients with AP and verify its efficacy in application.METHODS Through Delphi expert consultation,a narrative nursing model for negative emotions in patients with AP was constructed.A non-randomized quasi-experimental study design was used in this study.A total of 92 patients with AP with negative emotions admitted to a tertiary hospital in Nantong City of Jiangsu Province,China from September 2022 to August 2023 were recruited by convenience sampling,among whom 46 patients admitted from September 2022 to February 2023 were included in the observation group,and 46 patients from March to August 2023 were selected as control group.The observation group received narrative nursing plan,while the control group was given with routine nursing.Self-rating anxiety scale(SAS),self-rating depression scale(SDS),positive and negative affect scale(PANAS),caring behavior scale,patient satisfaction scale and 36-item short form health survey questionnaire(SF-36)were used to evaluate their emotions,satisfaction and caring behaviors in the two groups on the day of discharge,1-and 3-month following discharge.RESULTS According to the inclusion and exclusion criteria,a total of 45 cases in the intervention group and 44 cases in the control group eventually recruited and completed in the study.On the day of discharge,the intervention group showed significantly lower scores of SAS,SDS and negative emotion(28.57±4.52 vs 17.4±4.44,P<0.001),whereas evidently higher outcomes in the positive emotion score,Caring behavior scale score and satisfaction score compared to the control group(P<0.05).Repeated measurement analysis of variance showed that significant between-group differences were found in time effect,inter-group effect and interaction effect of SAS and PANAS scores as well as in time effect and inter-group effect of SF-36 scores(P<0.05);the SF-36 scores of two groups at 3 months after discharge were higher than those at 1 month after discharge(P<0.05).CONCLUSION The application of narrative nursing protocols has demonstrated significant effectiveness in alleviating anxiety,ameliorating negative emotions,and enhancing satisfaction among patients with AP.展开更多
目的了解综合医院门诊多躯体症状患者的躯体疾病和精神心理特点,并探讨其相关因素。方法 2012年3月至10月采用方便取样的方法,对北京协和医院消化内科、中医科和心理医学科门诊候诊患者通过躯体症状严重程度量表(somatic symptom scale ...目的了解综合医院门诊多躯体症状患者的躯体疾病和精神心理特点,并探讨其相关因素。方法 2012年3月至10月采用方便取样的方法,对北京协和医院消化内科、中医科和心理医学科门诊候诊患者通过躯体症状严重程度量表(somatic symptom scale of the Patient Health Questionnaire,PHQ-15)进行连续筛查。依据PHQ-15分数≥10或<10,将患者分为多躯体症状组(SOM+组)和对照组(SOM-组)。每科各组均纳入25例患者,共纳入150例患者。所纳入患者均完成7份自评问卷及结构化半定式访谈,包括过去12个月所患躯体疾病和简明国际神经精神访谈。结果 SOM+组患者女性(69.3%比53.3%)和低于大学文化程度者(54.8%比43.2%)所占比例较SOM-组更高(P<0.05)。SOM+组患者并未发现患有更多的可明确诊断的躯体疾病;而其抑郁发作、广泛性焦虑障碍和疑病症的患病率以及抑郁症状严重程度量表(depression scale of the Patient Health Questionnaire,PHQ-9)、广泛性焦虑量表(Generalized Anxiety Disorder 7-item scale,GAD-7)、健康焦虑量表(Whiteley-7 scale,WI-7)得分均显著高于SOM-组(P均<0.05),其日常生活和就医行为受症状影响更显著(P均<0.01)。对多躯体症状预测因素进行Logistic回归分析(P<0.01,调整R2=0.53),发现PHQ-9和WI-7总分高以及没有职业为影响因素。结论多躯体症状患者并未发现伴有更多可明确诊断的躯体疾病;此类患者的焦虑及抑郁程度更高,生活质量更低,且其症状对日常生活和就医影响更大;抑郁和健康焦虑水平高及没有职业是多躯体症状的预测因素。展开更多
BACKGROUND The brain is the most complex organ in the human body.Treatment for a glioma always involves a multi-disciplinary team.Nursing care in fast-track surgery or enhanced recovery after surgery is such kind of w...BACKGROUND The brain is the most complex organ in the human body.Treatment for a glioma always involves a multi-disciplinary team.Nursing care in fast-track surgery or enhanced recovery after surgery is such kind of work implemented by an interdisciplinary team to provide services to patients to improve their outcomes.AIM To explore the effects of nursing care in fast-track surgery on postoperative pain,psychological state,and patient satisfaction with nursing for glioma.METHODS From June 2018 to June 2020,138 patients who underwent operation for glioma at Cancer Hospital Affiliated to Chongqing University were selected.They were categorized into groups according to different nursing care that they received.Of them,69 patients receiving nursing care in fast-track surgery were included in an experimental group,and 69 patients receiving conventional postoperative nursing were included in a control group.Visual analogue scale was used to evaluate postoperative pain in the two groups immediately after the operation and at 3 d after the operation.Self-rating anxiety scale(SAS)and self-rating depression scale(SDS)were used to evaluate the psychological status of patients immediately after operation and on the 3rd postoperative day.A self-made satisfaction scale for patient satisfaction with nursing was used to evaluate and compare patient satisfaction with nursing between the two groups.RESULTS Time to excretion,time to out-of-bed activities,and length of hospital stay were significantly shorter in the observation group than in the control group(P<0.05).There was no significant difference in duration of operative time or intraoperative bleeding between the two groups(P>0.05).There was no significant difference in postoperative pain score between the two groups(P>0.05).The pain score was significantly lower in the observation group than in the control group at 3 d after the operation(P<0.05).There was no significant difference in postoperative SAS or SDS score between the two groups(P>0.05).SAS and SDS scores were significantly lower in the observation group than in the control group at 3 d after operation(P<0.05).The rate of patient satisfaction with nursing was 94.2%in the observation group,which was significantly higher than that(81.2%)of the control group(P<0.05).CONCLUSION Nursing care in fast-track surgery can relieve postoperative pain,anxiety,and depression,and improve patient satisfaction with nursing in patients with glioma,which is worthy of clinical application.展开更多
目的评价重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)联合度洛西汀在伴躯体症状老年抑郁症患者中的疗效。方法选取2018年12月至2019年12月住院治疗的伴躯体症状老年抑郁症患者186例,随机数表法随机分为对照组...目的评价重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)联合度洛西汀在伴躯体症状老年抑郁症患者中的疗效。方法选取2018年12月至2019年12月住院治疗的伴躯体症状老年抑郁症患者186例,随机数表法随机分为对照组及观察组,每组93例,分别采用度洛西汀及度洛西汀联合rTMS治疗。干预前及干预结束后2个月采用老年抑郁量表(GDS)、汉密尔顿焦虑量表(HAMA)、汉密尔顿抑郁量表(HAMD)、重复性成套神经心理状态测试(RBANS)以及不良反应量表(TESS)评价2组的干预效果,并采用临床疗效总量表评估治疗有效率。结果对照组和观察组治疗总有效率分别为93.55%及82.80%,观察组显著高于对照组,差异有统计学意义(P<0.05)。治疗前,2组患者治疗前HAMD评分、GDS评分及HAMA评分对比差异无统计学意义(P>0.05),治疗后上述指标均显著低于下降且观察组均显著低于对照组(P<0.05)。治疗前2组患者即刻记忆、视觉广度、言语、注意、延迟记忆等评分比较差异无统计学意义(P>0.05),治疗后上述指标均显著升高且观察组显著高于对照组(P<0.05)。治疗前2组患者TESS评分对比差异无统计学意义(P>0.05),治疗后TESS评分均显著降低且观察组显著低于对照组(P<0.05)。结论重复经颅刺激联合度洛西汀可以显著改善伴躯体症状的老年抑郁症患者的心理状况,具有较高的有效性和安全性,值得进一步推广。展开更多
文摘Abstract： Somatic embryogenesis from lily bulb scales has not been studied in details, although tissue culture methods have been applied to the propagation for decades. The effects of different kinds and concentration of auxins for oriental lily somatic embryogenesis were investigated （Lilium hybrida var. Sorbonne）. 2, 4-dichlorophenoxyacetic acid （2, 4-D）, thidiazuron （TDZ） and α-naphthaleneacetic acid （NAA） media with benzyladenine（6-BA） and lactalbumin hydrolysate （LH） were used for embryogenic callus in the darkness. The best response on embryogenic callus formation was obtained on MS media supplemented 2, 4-D 2.0 mg·L^-1, 6-BA 0.5 mg·L^-1 and LH 300 mg·L^-1. Transfer embryogenic callus to the media with TDZ, 6-BA, kinetin （KT） supplemented 2, 4-D. The highest number of somatic embryos has been produced on medium with 0.5 mg·L^-1 2, 4-D and 0.3 mg·L^-1 KT. Germinated embryos with shoot axes were changed to MS media with 6-BA 0.5 mg·L^-1. The results suggest that in vitro culture of somatic embryogenesis from lily bulb scales can be used for plant regeneration.
文摘Objective:The aim of the study is to investigate the relationship between antenatal depression and adverse perinatal outcomes.Methods:This prospective cohort study enrolled pregnant women between gestational ages of 12-20 weeks to complete the Chinese version of the Zung Self-Rating Depression Scale(SDS)and followed them for delivery from September 2015 to September 2016.Participants were classified into mild,moderate,and severe depression groups according to the SDS scores.Logistic regression was performed to assess the association between antenatal depression and perinatal outcomes including preterm birth(PTB),cesarean section use,hypertension disorders,gestational diabetes,and thyroid diseases during pregnancy.An age-stratified analysis was performed.Results:A total of 4,663 pregnant women were analyzed.As a result,13.8%,1.7%,and 0.2%of women were classified as mild,moderate,and severe depression,respectively.Severely depressed mothers were at higher risk for PTB(adjusted odds ratio[OR]=11.31,95%confidence interval[CI]2.13-60.03),especially spontaneous PTB.Moderate-depressed women were at higher risk for hyperthyroidism during pregnancy(adjusted OR=3.67,95%CI 1.10-12.27),while women with mild depression tended to choose cesarean sections(OR=1.24,95%CI=1.04-1.49).Age-stratified analysis indicated an elevated risk of adverse outcomes associated with depression in women aged<25 years,but the association was not significant.Conclusions:Antenatal depression was associated with PTB,hyperthyroidism,and cesarean use.Studies with large sample sizes should verify the relationship between PTB and antenatal depression to avoid casual events.
文摘BACKGROUND Acute pancreatitis(AP),as a common acute abdomen disease,has a high incidence rate worldwide and is often accompanied by severe complications.Negative emotions lead to increased secretion of stress hormones,elevated blood sugar levels,and enhanced insulin resistance,which in turn increases the risk of AP and significantly affects the patient's quality of life.Therefore,exploring the intervention effects of narrative nursing programs on the negative emotions of patients with AP is not only helpful in alleviating psychological stress and improving quality of life but also has significant implications for improving disease outcomes and prognosis.AIM To construct a narrative nursing model for negative emotions in patients with AP and verify its efficacy in application.METHODS Through Delphi expert consultation,a narrative nursing model for negative emotions in patients with AP was constructed.A non-randomized quasi-experimental study design was used in this study.A total of 92 patients with AP with negative emotions admitted to a tertiary hospital in Nantong City of Jiangsu Province,China from September 2022 to August 2023 were recruited by convenience sampling,among whom 46 patients admitted from September 2022 to February 2023 were included in the observation group,and 46 patients from March to August 2023 were selected as control group.The observation group received narrative nursing plan,while the control group was given with routine nursing.Self-rating anxiety scale(SAS),self-rating depression scale(SDS),positive and negative affect scale(PANAS),caring behavior scale,patient satisfaction scale and 36-item short form health survey questionnaire(SF-36)were used to evaluate their emotions,satisfaction and caring behaviors in the two groups on the day of discharge,1-and 3-month following discharge.RESULTS According to the inclusion and exclusion criteria,a total of 45 cases in the intervention group and 44 cases in the control group eventually recruited and completed in the study.On the day of discharge,the intervention group showed significantly lower scores of SAS,SDS and negative emotion(28.57±4.52 vs 17.4±4.44,P<0.001),whereas evidently higher outcomes in the positive emotion score,Caring behavior scale score and satisfaction score compared to the control group(P<0.05).Repeated measurement analysis of variance showed that significant between-group differences were found in time effect,inter-group effect and interaction effect of SAS and PANAS scores as well as in time effect and inter-group effect of SF-36 scores(P<0.05);the SF-36 scores of two groups at 3 months after discharge were higher than those at 1 month after discharge(P<0.05).CONCLUSION The application of narrative nursing protocols has demonstrated significant effectiveness in alleviating anxiety,ameliorating negative emotions,and enhancing satisfaction among patients with AP.
文摘目的了解综合医院门诊多躯体症状患者的躯体疾病和精神心理特点,并探讨其相关因素。方法 2012年3月至10月采用方便取样的方法,对北京协和医院消化内科、中医科和心理医学科门诊候诊患者通过躯体症状严重程度量表(somatic symptom scale of the Patient Health Questionnaire,PHQ-15)进行连续筛查。依据PHQ-15分数≥10或<10,将患者分为多躯体症状组(SOM+组)和对照组(SOM-组)。每科各组均纳入25例患者,共纳入150例患者。所纳入患者均完成7份自评问卷及结构化半定式访谈,包括过去12个月所患躯体疾病和简明国际神经精神访谈。结果 SOM+组患者女性(69.3%比53.3%)和低于大学文化程度者(54.8%比43.2%)所占比例较SOM-组更高(P<0.05)。SOM+组患者并未发现患有更多的可明确诊断的躯体疾病;而其抑郁发作、广泛性焦虑障碍和疑病症的患病率以及抑郁症状严重程度量表(depression scale of the Patient Health Questionnaire,PHQ-9)、广泛性焦虑量表(Generalized Anxiety Disorder 7-item scale,GAD-7)、健康焦虑量表(Whiteley-7 scale,WI-7)得分均显著高于SOM-组(P均<0.05),其日常生活和就医行为受症状影响更显著(P均<0.01)。对多躯体症状预测因素进行Logistic回归分析(P<0.01,调整R2=0.53),发现PHQ-9和WI-7总分高以及没有职业为影响因素。结论多躯体症状患者并未发现伴有更多可明确诊断的躯体疾病;此类患者的焦虑及抑郁程度更高,生活质量更低,且其症状对日常生活和就医影响更大;抑郁和健康焦虑水平高及没有职业是多躯体症状的预测因素。
文摘BACKGROUND The brain is the most complex organ in the human body.Treatment for a glioma always involves a multi-disciplinary team.Nursing care in fast-track surgery or enhanced recovery after surgery is such kind of work implemented by an interdisciplinary team to provide services to patients to improve their outcomes.AIM To explore the effects of nursing care in fast-track surgery on postoperative pain,psychological state,and patient satisfaction with nursing for glioma.METHODS From June 2018 to June 2020,138 patients who underwent operation for glioma at Cancer Hospital Affiliated to Chongqing University were selected.They were categorized into groups according to different nursing care that they received.Of them,69 patients receiving nursing care in fast-track surgery were included in an experimental group,and 69 patients receiving conventional postoperative nursing were included in a control group.Visual analogue scale was used to evaluate postoperative pain in the two groups immediately after the operation and at 3 d after the operation.Self-rating anxiety scale(SAS)and self-rating depression scale(SDS)were used to evaluate the psychological status of patients immediately after operation and on the 3rd postoperative day.A self-made satisfaction scale for patient satisfaction with nursing was used to evaluate and compare patient satisfaction with nursing between the two groups.RESULTS Time to excretion,time to out-of-bed activities,and length of hospital stay were significantly shorter in the observation group than in the control group(P<0.05).There was no significant difference in duration of operative time or intraoperative bleeding between the two groups(P>0.05).There was no significant difference in postoperative pain score between the two groups(P>0.05).The pain score was significantly lower in the observation group than in the control group at 3 d after the operation(P<0.05).There was no significant difference in postoperative SAS or SDS score between the two groups(P>0.05).SAS and SDS scores were significantly lower in the observation group than in the control group at 3 d after operation(P<0.05).The rate of patient satisfaction with nursing was 94.2%in the observation group,which was significantly higher than that(81.2%)of the control group(P<0.05).CONCLUSION Nursing care in fast-track surgery can relieve postoperative pain,anxiety,and depression,and improve patient satisfaction with nursing in patients with glioma,which is worthy of clinical application.
文摘目的评价重复经颅磁刺激(repetitive transcranial magnetic stimulation,rTMS)联合度洛西汀在伴躯体症状老年抑郁症患者中的疗效。方法选取2018年12月至2019年12月住院治疗的伴躯体症状老年抑郁症患者186例,随机数表法随机分为对照组及观察组,每组93例,分别采用度洛西汀及度洛西汀联合rTMS治疗。干预前及干预结束后2个月采用老年抑郁量表(GDS)、汉密尔顿焦虑量表(HAMA)、汉密尔顿抑郁量表(HAMD)、重复性成套神经心理状态测试(RBANS)以及不良反应量表(TESS)评价2组的干预效果,并采用临床疗效总量表评估治疗有效率。结果对照组和观察组治疗总有效率分别为93.55%及82.80%,观察组显著高于对照组,差异有统计学意义(P<0.05)。治疗前,2组患者治疗前HAMD评分、GDS评分及HAMA评分对比差异无统计学意义(P>0.05),治疗后上述指标均显著低于下降且观察组均显著低于对照组(P<0.05)。治疗前2组患者即刻记忆、视觉广度、言语、注意、延迟记忆等评分比较差异无统计学意义(P>0.05),治疗后上述指标均显著升高且观察组显著高于对照组(P<0.05)。治疗前2组患者TESS评分对比差异无统计学意义(P>0.05),治疗后TESS评分均显著降低且观察组显著低于对照组(P<0.05)。结论重复经颅刺激联合度洛西汀可以显著改善伴躯体症状的老年抑郁症患者的心理状况,具有较高的有效性和安全性,值得进一步推广。
