Quantitative studies of the regular distribution pattern for Salmonella enteritidis in the internal organs of mice after oral challenge by a specific real-time polymerase chain reaction

AIM:To identify and understand the regular distribution pattern for Salmonella enteritidis (S. enteritidis) in the internal organs of mice after an oral challenge over a 3 wk period. METHODS:Assays based on the serovar-specific DNA sequence of S. enteritidis from GenBank, and a serovar-specific real-time, fluorescence-based quantitative polymerase chain reaction (FQ-PCR) were developed for the detection of S. enteritidis. We used this assay to detect genomic DNA of S. enteritidis in the blood and the internal organs, including heart, liver, spleen, kidney, pancreas, and gallbladder, from mice after oral challenge at different time points respectively.RESULTS:The results showed that the spleen was positive at 12 h post inoculation (PI), and the blood was at 14 h PI. The organism was detected in the liver and heart at 16 h PI, the pancreas was positive at 20 h PI, and the final organs to show positive results were the kidney and gallbladder at 22 h PI. The copy number of S. enteritidis DNA in each tissue reached a peak at 24-36 h PI, with the liver and spleen containing high concentrations of S. enteritidis, whereas the blood, heart, kidney, pancreas, and gallbladder had low concentrations. S. enteritidis populations began to decrease and were not detectable at 3 d PI, but were still present up to 12 d PI in the gallbladder, 2 wk for the liver, and 3 wk for the spleen without causing apparent symptoms. CONCLUSION:The results provided significant data for understanding the life cycle of S. enteritidis in the internal organs, and showed that the liver and spleen may be the primary sites for setting itself up as a commensa over a long time after oral challenge. Interestingly, it may be the first time reported that the gallbladder is a site of carriage for S. enteritidis over a 12 d period. This study will help to understand the mechanisms of action of S. enteritidis infection in vivo.

Development of a Porcine cDNA Microarray:Analysis of Clenbuterol Responding Genes in Pig(Sus scrofa) Internal Organs

Pig （Sus scrofa） fat accumulation can be reduced by feeding with high dosages of clenbuterol, but the molecular mechanism has not yet been explained. In our study, a porcine cDNA microarray representing 3 358 pig genes was successfully developed. This microarray is the first porcine DNA microarray in China and its false positive rate is 0.98%, which means the microarray platform is reliable. The microarray can be used to study gene expression profiles in multiple pig tissues because the present genes percentage of adipose, skeletal muscle, heart, liver, lung, kidney, and spleen were all more than 60%. This microarray was used to identify the genes responding to clenbuterol stimulation in pig internal organs, including heart, liver, lung, spleen, and kidney. Many genes were identified including enzymes involved in lipids metabolism （lipoprotein lipase up-regulated in liver, heart and lung, ATP-citrate lyase and carnitine palmitoyltransferase II precursor up-regulated in liver, succinyl-CoA up-regulated in lung, mitochondrial malate dehydrogenase down-regulated in spleen）, and signaling pathway genes （cAMP-protein kinase A signaling pathway was found up-regulated in liver, heart, lung, and kidney as reported previously, while transforming growth factor was found down-regulated in heart and lung）. However, no common gene responding to clenbuterol administration was found in all tissues. The expression levels of 14 genes were analyzed using real-time PCR with 82.1% of them induced to express similar magnitudes as in the microarray analyses. This work offers some understanding of how clenbuterol so effectively reduces pig adipose accumulation on the molecular level.

Effects of phytase supplementation on growth performance, slaughter performance, growth of internal organs and small intestine, and serum biochemical parameters of broilers

The objective of this study was to investigate the effects of phytase supplementation on growth performance, slaughter performance, growth of internal organs and small intestine, and serum biochemical indices of broilers. A total of 360 1-day-old Ross 308 broilers were randomly divided into 4 groups with 3 replicates and 30 broilers per replicate (15 male, 15 female). The treatments were fed with basal diet supplemented with 0%, 0.01%, 0.02% and 0.03% phytase. The results showed that: 1) Phytase supplementation increased the body weight gain and the body weight of Ross 308 broilers (P serum calcium (Ca) consistency was 0.02%. According to the above analysis, the feasible supplementation of phytase (enzyme activity for 5000 u/g) in broiler dietary was 0.02%.

Preliminary Measurement of Internal Organs of Congjiang Fragrance Pig and Huanjiang Fragrance Pig

Fragrance pig is a famous miniature local breed in China,which is similar to human on the aspects of physical structure,anatomy,nutrition,metabolism and blood biochemical indicators. The internal organs of Congjiang fragrance pig and Huanjiang fragrance pig with different month ages were weighed. The results showed that the proportion of stomach in body weight in Congjiang fragrance pig was higher than that in Min pig,Harbin white and Landrace,and the proportion of large intestine in body weight in Congjiang fragrance pig was also higher than that in ordinary pigs; the weights of heart,liver and kidney in 8- 10 months old Congjiang fragrance pig were similar to that in Chinese adults. This provided reference data for future in-depth development and utilization of fragrance pig.

Enhance the Influence of China-UK “One Belt and One Road” Cooperation

The Belt and Road Initiative is a new concept of opening-up put forward by Chinese leader Xi Jinping after he took office.It is the most influential international initiative since China’s reform and opening up.Its implementation has achieved remarkable results in the past few years,with more than expected results.More and more countries in the world have supported and responded to the Belt and Road Initiative.China and the UK are countries with important influence in the world.The Belt and Road Initiative has great potential and far-reaching impact between two countries.This paper analyzes the theory of influence of the Belt and Road and discusses the practical problems existing in the China-UK Belt and Road Cooperation.By applying the research method of matching theory with reality,the author puts forward some measures to enhance the influence of the Belt and Road Cooperation between the two countries.It is of great academic and practical significance to study the Belt and Road Theory and the international relations between China and Britain.

Sets up scientific development views, pushes the construction of the Dalian Northeast Asia international shipping center

this article elaborated the important theoretic and practical significance which the strategic decision proposed by The Central Committee of the CCP and the State Council to constructed Dalian city the Northeast Asia important international shipping center manifested; moreover, put forward in the face of the historic opportunity, how to insist on the scientific development view in order to impel the construction of the Dalian international shipping center.

The 13th Humen International Fashion Fair The View of a Gorgeous Back

Cheerful songs and passionate dance bring out the best of all. In a sea of harvest celebration, the 13th Humen International Fashion Fair concluded with a complete success.During only 5 days from Nov. 21 to 25, the famous fashion city Humen became a

基于Photo Sphere Viewer的轻量化可量测街景系统研究

随着新型基础测绘体系发展,测绘技术和装备不断升级,大量街景数据随着车载激光扫描工作一并被采集。针对该数据路线随机性大、密集程度高等特点,以及传统街景地图系统插件受限、扩展性不强、无法量测等局限,本文设计了基于Photo Sphere Viewer的轻量化可量测街景系统,在开源插件的基础上实现了对其组件的扩充、实现了行进方向、地图交互等系统功能的设计优化,进一步实现了基于深度图的街景地图量测功能。生产作业队伍对系统的使用结果表明,该系统可以辅助生产作业队伍进行属性采集、纹理更新、数据检查等多项工作,操作灵活便捷,且具有较强的可扩展性,便于基于生产需求进行改进调整,提高了生产效率,为新型基础测绘成果进一步深化应用提供了良好的借鉴作用。

Constructing global view with an ontology-based method for information sharing in the virtual organization

The problem of constructing global view of heterogeneous information sources for information sharing is becoming more and more important due to the availability of multiple information sources within the virtual organization. Global view is defined to provide a unified representation of the information in the different sources by analyzing concept schemas associated with them and resolving possible semantic heterogeneity. An ontology-based method for global view construction is proposed. In the method, （ 1 ） Based on the formal ontologies, the concept of semantic affinity is introduced to assess the level of semantic relationship between information classes from different information sources; （2） Information classes are classified by semantic affinity levels using clustering procedures so that their different representations can be analyzed for unification; （3） Global view is constructed starting from selected clusters by unifying representation of their elements. The application example of using the method in the joint-aerial defense organization is illustrated and the result shows that the proposed method is feasible.

Exergy Analysis of Organic Rankine Cycles with Zeotropic Working Fluids

Waste heat recovery is one of the possible solutions to improve the efficiency of internal combustion engines.Instead of wasting the exhaust stream of an energy conversion system into the environment,its residual energy content can be usefully recovered,for example in Organic Rankine Cycles(ORC).This technology has been largely consolidated in stationary power plants but not yet for mobile applications,such as road transport,due to the limitations in the layout and to the constraints on the size and weight of the ORC system.An ORC system installed on the exhaust line of a bus powered by a natural gas spark ignition engine has been investigated.The thermal power available at engine exhaust has been evaluated by measuring gas temperature and mass flow rate during real driving operation.The waste thermal power has been considered as heat input for the ORC plant simulation.A detailed heat exchanger model has been developed because it is a crucial component for the ORC performance.The exergy analysis of the ORC was performed comparing different working fluids:R601,R1233zd(E)and two zeotropic blends of the two organic pure fluids.The model allowed the evaluation of the ORC produced energy over the driving cycle and the potential benefit on the engine efficiency.

Minimizing the risk of international spread of coronavirus disease 2019 (COVID-19) outbreak by targeting travelers

The Coronavirus Disease-2019 (COVID-19) outbreak has become a global health emergency owing to its magnitude, attributed deaths, and its propensity to spread across the world. In-fact, owing to its quick spread across international boundaries and the resulting caseload, the disease has been declared as a Public Health Emergency of International Concern on 30 January 2020. It is worth noting that out of the 395 cases detected in other nations, 165 (41.8%) have a positive history of travel to China. As of now, the World Health Organization has not recommended for any restrictions on the travel or trade aspects, but has clearly specified that implementation of International Health Regulations should be strictly done at the airports and seaports. In conclusion, the COVID-19 outbreak has created an alarm across the globe as the causative virus is novel in nature. However, strengthening of standard infection control practices and adoption of preventive measures for travelers can significantly minimize the threat of further transmission of the disease.

DISTRIBUTION OF SOME ELEMENTS IN HUMAN HAIR AND ORGANS DETERMINED BY NAA

Twenty four male autopsies were obtained from Shanghai. The samples of liver, lung, kidney cortex, brain and scalp hair were collected from the autopsies. The elements As, Br, Ca, Cd, Cl, Co, Cr, Cu, Fe, Hg, Mg, Mn, Na, Rb, 8, Se and Zn were determined by neutron activation analysis (NAA). The present study provides the elemental levels of different tissues for normal male adults. The distribution pattern of elements was discussed. Great difference on distribution of some elements in different tissues was observed.

Performance analysis and improvement of geothermal binary cycle power plant in oilfield

In order to improve the efficiency of a geothermal power plant, oil wells in the high water cut stage were used as geothermal wells, thereby improving the recovery ratio and economic benefit. A new function that reflects both the technical and economic performances was put forward and used as the objective function. An organic Rankine cycle (ORC) was analyzed through the energetic and exergetic analyses, and the reasons for low efficiency were pinpointed. Results indicate that geothermal water directly transferring heat to the working fluid reduces energy dissipation and increases cycle efficiencies. The net power output with an internal heat exchanger (IHE) is averagely 5.3% higher than that without an IHE. R601a and R601 can be used to replace R123 for geothermal water below 110℃. Moreover, the modified ORC dramatically outperforms the actual one.

Social and Economic Considerations for Creating Sustainable Climate Change Haven Communities

As Climate Change Haven Communities are constructed across the Northern Hemisphere, it will be necessary to attract two types of migrants to populate them. The first group consists of professionals and companies in eco-sustainable businesses, such as law firms, insurance companies, investment firms, banking, technological innovation, mass media, medical research and pharmaceutical research. The second group will consist of persons engaged in organic/eco-sustainable agriculture whose crops and animal husbandry practices can be transferred successfully to Climate Change Haven regions. The present research focuses on the social and economic variables that must be taken into account to insure that each new Climate Change Haven Community becomes successfully integrated with the local population and forms a cohesive, harmonious social structure. Examples are given from the United States, France, Spain, Portugal and Italy.

Application of Organic Growth Views in Residential Spaces

This paper explored relevant contents, methods and comprehensive efficiency of organic growth view in residential spaces based on personal experience, so as to fi gure out a new approach of residential space design with high economic efficiency, participation and sustainable connotations.

Research on governance of HFO use and carriage on ships in accordance with the Polar Code

In this paper, we discuss the environmental damages caused by the use and carriage of Heavy Fuel Oil(HFO) by ships in Arctic waters. We also review efforts made by major international and regional organizations in governing the use and carriage of HFO by ships in Arctic waters after implementation of the Polar Code, and analyze the obstacles and difficulties that lie ahead. By illustrating the features of the Arctic Council and the International Maritime Organization(IMO), which govern Arctic waters, we examine measures taken by these two organizations in tackling HFO issues. These include: assessing indigenous and local community’s reliance on HFO transportation in the Arctic, developing risk prevention measures for ships operating in Arctic waters, seeking economic alternatives to HFO that contribute to a greener economy, developing a package of HFO governance measures, strengthening cooperation between relevant international and regional organizations that govern HFO to provide suggestions for follow-up discussions on HFOs, and optimize governance by relevant organizations and determine a better global solution to governing the use and carriage of HFO by ships. In addition, we discuss the feasibility of an IMO-imposed HFO ban from the perspective of the Arctic governance to clearly grasp the path of its future development.

The Second International Conference on HSLA Steels——Beijing,China,October 1990 Organized by THE CHINESE SOCIETY OF METALS

GENERAL INFORMATIONThe Chinese Society of Metals is pleased to announcethat The Second International Conference on HSLASteels (HSLA’90) will be held in Beijing,China inOctober 1990.Much progress in research and devel-opment of HSLA steels has been achieved by theworld’s metallurgical and materials community since