In this study,fucoidans were extracted from the sea cucumber Thelenota ananas(Ta-FUCs)by enzymatic degradation.Four products with molecular weights of 1380.3,524.0,182.4,and 110.3 kDa were obtained,and the Ta-FUC show...In this study,fucoidans were extracted from the sea cucumber Thelenota ananas(Ta-FUCs)by enzymatic degradation.Four products with molecular weights of 1380.3,524.0,182.4,and 110.3 kDa were obtained,and the Ta-FUC showing optimal anti-adipogenic activities was determined.Results of MTT and Oil red O staining analyses showed that the Ta-FUCs inhibited the proliferation and differentiation of 3T3-L1 adipocytes.Futhermore,Ta-FUCs significantly downregulated the key transcriptional factors,such as SREBP-1c,PPARγ,and C/EBPαof adipocytes.The Ta-FUCs also activated Wnt/β-catenin pathway-related genes,such asβ-catenin,LRP5,and FrZ.The Ta-FUCs suppressed lipid accumulation in 3T3-L1 adipocytes possibly by decreasing the expression of genes ACC,FAS,ME,GPAT,DGAT,and PILN,which are important in the synthesis of fatty acids and triglycerides;and by increasing the expression of genes PPARα,CPT-1α,and ACOX,which are crucial in fatty acidβ-oxidation.The anti-adipogenic activities initially increased and then declined with decreasing molecular weight.Among the Ta-FUCs,the 182.4 kDa Ta-FUC exhibited optimal bioactivities.This study reports for the first time that Ta-FUCs can prevent obesity by regulating the differentiation and lipid accumulation of adipocytes.展开更多
目的研究梅花参体内的化学成分。方法利用DA101大孔树脂,硅胶,ODS Rp C-18反相硅胶．Sephadex LH-20柱色谱和HPLC方法进行分离纯化,根据所得化合物的理化性质和波谱与质谱数据(尤其是2D-NMR和ESI—MS技术),鉴定其结构。结果得到4个化合...目的研究梅花参体内的化学成分。方法利用DA101大孔树脂,硅胶,ODS Rp C-18反相硅胶．Sephadex LH-20柱色谱和HPLC方法进行分离纯化,根据所得化合物的理化性质和波谱与质谱数据(尤其是2D-NMR和ESI—MS技术),鉴定其结构。结果得到4个化合物,分别鉴定为:holothuria A(1),胸腺嘧啶(2),尿嘧啶(3),胆甾醇(4)。结论4个化合物均为首次从该种动物中得到。展开更多
基金This study was supported by the National Key Research and Development Program of China(No.2018YFC0311203)the Key Research and Development Plan in Shandong Province(No.2016YYSP017).
文摘In this study,fucoidans were extracted from the sea cucumber Thelenota ananas(Ta-FUCs)by enzymatic degradation.Four products with molecular weights of 1380.3,524.0,182.4,and 110.3 kDa were obtained,and the Ta-FUC showing optimal anti-adipogenic activities was determined.Results of MTT and Oil red O staining analyses showed that the Ta-FUCs inhibited the proliferation and differentiation of 3T3-L1 adipocytes.Futhermore,Ta-FUCs significantly downregulated the key transcriptional factors,such as SREBP-1c,PPARγ,and C/EBPαof adipocytes.The Ta-FUCs also activated Wnt/β-catenin pathway-related genes,such asβ-catenin,LRP5,and FrZ.The Ta-FUCs suppressed lipid accumulation in 3T3-L1 adipocytes possibly by decreasing the expression of genes ACC,FAS,ME,GPAT,DGAT,and PILN,which are important in the synthesis of fatty acids and triglycerides;and by increasing the expression of genes PPARα,CPT-1α,and ACOX,which are crucial in fatty acidβ-oxidation.The anti-adipogenic activities initially increased and then declined with decreasing molecular weight.Among the Ta-FUCs,the 182.4 kDa Ta-FUC exhibited optimal bioactivities.This study reports for the first time that Ta-FUCs can prevent obesity by regulating the differentiation and lipid accumulation of adipocytes.
文摘目的研究梅花参体内的化学成分。方法利用DA101大孔树脂,硅胶,ODS Rp C-18反相硅胶．Sephadex LH-20柱色谱和HPLC方法进行分离纯化,根据所得化合物的理化性质和波谱与质谱数据(尤其是2D-NMR和ESI—MS技术),鉴定其结构。结果得到4个化合物,分别鉴定为:holothuria A(1),胸腺嘧啶(2),尿嘧啶(3),胆甾醇(4)。结论4个化合物均为首次从该种动物中得到。
