Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury

A preliminary study from our research group showed that picroside II inhibited neuronal apop- tosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. The aim of the present study was to validate the neuroprotective effects of picroside II and optimize its therapeutic time window and dose in a rat model of cerebral ischemia. We found that picroside Ⅱ inhibited cell apoptosis and reduced the expression of neuron-specific enolase, a marker of neuronal damage, in rats after cerebral ischemic injury. The optimal treatment time after ischemic injury and dose were determined, respectively, as follows： （1） 2.0 hours and 10 mg/kg according to the results of toluidine blue staining; （2） 1.5 hours and 10 mg/kg according to early apoptotic ratio by flow cytometry; （3） 2.0 hours and 10 mg/kg according to immunohistochemical and western blot analysis; and （4） 1.5 hours and 10 mg/kg according to reverse transcription polymerase chain reaction. The present findings suggest that an intraperitoneal injection of 10 mg/kg picroside II 1.5-2.0 hours after cerebral ischemic injury in rats is the optimal dose and time for therapeutic benefit.

Experimental verification of therapeutic doses for the superficially-placed tumor radiotherapy with heavy ions at HIRFL

Up to now, clinical trials of heavy-ion radiotherapy for superficially placed tumors have been carried out for six times and over 60 selected patients have been treated with 80--100 MeV/u carbon ions supplied by the Heavy Ion Research Facility in Lanzhou （HIRFL） at the Institute of Modern Physics, Chinese Academy of Sciences since November, 2006. A passive irradiation system and a dose optimization method for radiotherapy with carbon-ion beams have been developed. Experimental verification of longitudinally therapeutic dose distributions was conducted under the condition of simulating patient treatment in the therapy terminal at HIRFL. The measured depth-dose distributions basically coincide with the expected ones. These results indicate that the irradiation system and the dose optimization method are effective in the ongoing carbon-ion radiotherapy for shallow-seated tumors at HIRFL.

Professor Jia Chunsheng's instruction on clinical application of"elements for acupuncture effect orientation"in the Song to Elucidate Mysteries

Professor Jia Chunsheng is one of the sixth of old traditional Chinese medicine experts,and often instructs students in ordinary clinics to attach importance to learning and clinical practices of classic works.Now Professor Jia Chunsheng selects a poet-Song to Elucidate Mysteries in the Guidelines of Acupuncture Classic by Dou Hanqing in the Jin and Yuan Dynasties,and requires the students to read carefully,investigate deeply,and apply at times;Moreover,Professor Jia Chunsheng discusses the"important factors to achieve good clinical therapeutic effects by acupuncture"contained in the Song to Elucidate Mysteries,in many aspects of understanding acupoint characteristics,defining clearly acupoint compatibility,selecting appropriate needling manipulation,how to determine needling depth,how to determine needling direction,how to determine needle retention time,and how to select acupuncture&moxibustion method,etc.Now they are discussed as follows,to discover and spread Dou's acupuncture technique and to increase clinical therapeutic effects.