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Flexible interwoven termini determine the thermal stability of thermosomes
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作者 Kai Zhang Li Wang +5 位作者 Yanxin Liu Kwok-Yan Chan Xiaoyun Pang Klaus Schulten Zhiyang Dong Fei Sun 《Protein & Cell》 SCIE CSCD 2013年第6期432-444,共13页
Group II chaperonins,which assemble as double-ring complexes,assist in the refolding of nascent peptides or denatured proteins in an ATP-dependent manner.The mo-lecular mechanism of group II chaperonin assembly and th... Group II chaperonins,which assemble as double-ring complexes,assist in the refolding of nascent peptides or denatured proteins in an ATP-dependent manner.The mo-lecular mechanism of group II chaperonin assembly and thermal stability is yet to be elucidated.Here,we selected the group II chaperonins(cpn-αand cpn-β),also called thermosomes,from Acidianus tengchongensis and in-vestigated their assembly and thermal stability.We found that the binding of ATP or its analogs contributed to the successful assembly of thermosomes and enhanced their thermal stabilities.Cpn-βis more thermally stable than cpn-α,while the thermal stability of the hetero thermo-some cpn-αβis intermediate.Cryo-electron microscopy reconstructions of cpn-αand cpn-βrevealed the interwo-ven densities of their non-conserved fl exible N/C-termini around the equatorial planes.The deletion or swapping of their termini and pH-dependent thermal stability assays revealed the key role of the termini electrostatic interac-tions in the assembly and thermal stability of the ther-mosomes. 展开更多
关键词 group II chaperonin thermosome thermal stability SELF-ASSEMBLY fl exible terminus
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Structural variation of molecular chaperone group Ⅱ by crystallography and cryo-electron microscopy
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作者 Gabe Lander 孙飞 《生物物理学报》 CAS CSCD 北大核心 2009年第S1期7-8,共2页
Chaperonins, a class of molecular chaperones, are oligomeric complexes acting as a protein-folding chamber in an ATP-dependent manner. Chaperonins have been classifed
关键词 CHAPERONIN thermosome cryo-electron MICROSCOPY SYMMETRY CONFORMATION change
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