目的:提高宫颈癌筛查的诊断率。方法:采用宫颈管刷分别收集宫颈外口和宫颈管的脱落细胞,将传统巴氏涂片和膜式液基薄层(TCT)两种方法作双盲比较,然后两组对宫颈有病变的患者做阴道镜组织活检,有异常图像者,取组织病理,最终作评估。结果...目的:提高宫颈癌筛查的诊断率。方法:采用宫颈管刷分别收集宫颈外口和宫颈管的脱落细胞,将传统巴氏涂片和膜式液基薄层(TCT)两种方法作双盲比较,然后两组对宫颈有病变的患者做阴道镜组织活检,有异常图像者,取组织病理,最终作评估。结果:两组经阴道检查在2 981例中TCTCIN I 56例,CIN II 27例,CIN III 9例,SCC 6例;巴氏CIN I 21例,CIN II 13例,CIN III 4例,SCC 2例。TCT与传统巴氏检出率比较明显高于传统巴氏涂片。结论:宫颈液基标本收集方法有利于细胞学和肿瘤相关HPV、DNA双重检查。与巴氏细胞学涂片技术相比ThinPrep液基细胞学检查敏感性高,使发现低度和高度病变的敏感度提高。展开更多
A new modification method for glass slides was developed and applied to make ThinPrep Pap smears,in order to increase the adhesion ability of cervical exfoliative cells.3-glycidyloxypropyl trimethoxysilane(GOPS) was c...A new modification method for glass slides was developed and applied to make ThinPrep Pap smears,in order to increase the adhesion ability of cervical exfoliative cells.3-glycidyloxypropyl trimethoxysilane(GOPS) was coated on the glass slides firstly on the slides,then poly-L-lysine(PLL) was covalently modified onto the above epoxy-terminated slides to form GOPS-PLL double decorated slides.The modified slides were characterized using X-ray photoelectron spectroscopy(XPS) and atomic force microscopy(AFM).The cell adhesion ability effect was tested and compared with traditional PLL coated slides by fixing the cervical exfoliative cells on the double adorned slides.The control test was conducted by the bare glass slides unmodified.The cell morphology of cervical exfoliative cells adhered on different slides was observed under the microscope after Papanicolaou staining.The number of cervical exfoliative cells on the unmodified slides,PLL coated slides and GOPS-PLL coated slides was 10307300,32837226 and 41197280(n=12),respectively.The data among the three different modification methods showed significant differences(one-way analysis of variance,ANOVA test,Po0.05).The cell capturing effect of the GOPS-PLL slide was the best among the three different modified slides.In addition,the GOPS-PLL slide could enhance the uniformity of the adhered cells and be widely applied to the ThinPrep system for cervical carcinoma screening to increase the accuracy rate of diagnosis.展开更多
文摘目的:提高宫颈癌筛查的诊断率。方法:采用宫颈管刷分别收集宫颈外口和宫颈管的脱落细胞,将传统巴氏涂片和膜式液基薄层(TCT)两种方法作双盲比较,然后两组对宫颈有病变的患者做阴道镜组织活检,有异常图像者,取组织病理,最终作评估。结果:两组经阴道检查在2 981例中TCTCIN I 56例,CIN II 27例,CIN III 9例,SCC 6例;巴氏CIN I 21例,CIN II 13例,CIN III 4例,SCC 2例。TCT与传统巴氏检出率比较明显高于传统巴氏涂片。结论:宫颈液基标本收集方法有利于细胞学和肿瘤相关HPV、DNA双重检查。与巴氏细胞学涂片技术相比ThinPrep液基细胞学检查敏感性高,使发现低度和高度病变的敏感度提高。
基金supported by National 863 Program (no.2007AA09210107)
文摘A new modification method for glass slides was developed and applied to make ThinPrep Pap smears,in order to increase the adhesion ability of cervical exfoliative cells.3-glycidyloxypropyl trimethoxysilane(GOPS) was coated on the glass slides firstly on the slides,then poly-L-lysine(PLL) was covalently modified onto the above epoxy-terminated slides to form GOPS-PLL double decorated slides.The modified slides were characterized using X-ray photoelectron spectroscopy(XPS) and atomic force microscopy(AFM).The cell adhesion ability effect was tested and compared with traditional PLL coated slides by fixing the cervical exfoliative cells on the double adorned slides.The control test was conducted by the bare glass slides unmodified.The cell morphology of cervical exfoliative cells adhered on different slides was observed under the microscope after Papanicolaou staining.The number of cervical exfoliative cells on the unmodified slides,PLL coated slides and GOPS-PLL coated slides was 10307300,32837226 and 41197280(n=12),respectively.The data among the three different modification methods showed significant differences(one-way analysis of variance,ANOVA test,Po0.05).The cell capturing effect of the GOPS-PLL slide was the best among the three different modified slides.In addition,the GOPS-PLL slide could enhance the uniformity of the adhered cells and be widely applied to the ThinPrep system for cervical carcinoma screening to increase the accuracy rate of diagnosis.
