The process of rice(Oryza sativa L.)seedling cultivation is often subjected to adverse environmental stress.Biostimulants regulate the robust growth of rice seedlings and play a crucial role in promoting the green and...The process of rice(Oryza sativa L.)seedling cultivation is often subjected to adverse environmental stress.Biostimulants regulate the robust growth of rice seedlings and play a crucial role in promoting the green and ecological development of agriculture.In this study,1.0 and 2.0 g•m^(-2) of the biostimulant were applied to soil in rice seedbeds.Growth indicators of rice,antioxidant enzyme activities and soil physicochemical characteristics were assessed at the 2.5-leaf and 4-leaf stages of rice.The results indicated that applying 2.0 g•m^(-2) of the biostimulant at both the 2.5-leaf and 4-leaf stages had the most significant promoting effect on rice growth.At the 2.5-leaf and 4-leaf stages,the number of fibrous roots increased by 23.43%and 22.25%,stem base width increased by 19.05%and 19.58%,above ground dry weight increased by 18.09%and 16.47%,root dry weight increased by 19.67%and 18.28%,leaf peroxidase(POD)activity increased by 34.44%and 42.94%,superoxide dismutase(SOD)activity increased by 37.24%and 56.79%,malondialdehyde(MDA)content decreased by 18.60%and 27.67%,and chlorophyll content increased significantly by 28.31%and 34.24%,respectively.At the 4-leaf stage of rice,urease,phosphatase and cellulase activities in the seedbed soil increased by 42.13%,25.96%and 33.59%,respectively,while soil alkaline nitrogen,available phosphorus and available potassium content decreased by 19.76%,19.02%and 17.88%,respectively.The application of biostimulants played a crucial role in promoting the growth of rice seedlings and enhancing soil nutrient absorption.展开更多
The research used bioassay method to explore seed tolerance of Cd in terms of seed germination and seedling growth. Specifically, 12 rice varieties of three types were investigated under Cd stress, covering germinatio...The research used bioassay method to explore seed tolerance of Cd in terms of seed germination and seedling growth. Specifically, 12 rice varieties of three types were investigated under Cd stress, covering germination rate, shooting index, vigor index, seedling and shoot lengths, and fresh weights. The results showed that as Cd concentration grew, seed germination and seedling growth kept increasingly inhibited; germination index, vigor index, root length, shoot length and fresh weights went down; inhibition effects on seed germination and seedling growth were conventional rice〉two-line hybrid rice〉three-line hybrid ,rice. Shanyou 9588, Tianyou 998, Chuanxiang 8, and Y Liangyou 1 performed ~rongly in Cd tolerance; C Liangyou 651, Nei 5 You 263, Xiangwanxian 16, Huanghuazhan, Xiangwanxian 13, and Zhunliangyou 527 performed poorly in Cd tolerance, and Cd tolerance of rest treatments were moderate.展开更多
The effects of farming method, rice variety and seedling-raising method were studied on the population growth quality and grain yield of the mechanized transplanting rice by way of three-factor split plot design. The ...The effects of farming method, rice variety and seedling-raising method were studied on the population growth quality and grain yield of the mechanized transplanting rice by way of three-factor split plot design. The results showed that the no-tillage mechanical transplanting treatment was poorer than the conventional mechanical transplanting treatment in transplanting quality in general, but the former was similar to the latter in population growth quality. In regard to grain yield, the no-tillage mechanical transplanting treatment was lower than the conventional me- chanical transplanting treatment. Raising both pot-mat seedling and plastic-tray seedling did not make much difference in population growth and grain yield of mechanized transplanting rice, so both of the seedling-raising methods can be adopted according to practical conditions.展开更多
Understanding the characteristics of rice productivity is of great importance for achieving high yield formation.However,such traits have not yet been studied for different ages of hydroponically grown long-mat rice s...Understanding the characteristics of rice productivity is of great importance for achieving high yield formation.However,such traits have not yet been studied for different ages of hydroponically grown long-mat rice seedlings(HLMS),which constitutes a new method of seedling cultivation.Field experiments were conducted to evaluate the effects of seedling age on the growth stage,photosynthesis characteristics,dry matter production,and yield of HLMS.A conventional japonica rice cultivar(Wuyunjing 24)and an indica hybrid rice cultivar(6 Liangyou 9368)were used as test materials.The results showed that the whole phase was shortened by 13-15 days for young seedlings(13-day-old)compared with old seedlings(27-day-old),which occurred because the growth process accelerated with the transplantation of young seedlings.As seedling age increased,the dry matter weight of stems of individual plants and of the population increased at the transplanting stage but decreased at the maturity stage(MS).Compared with that of 27-day-old seedlings,the average ratio of panicle weight to total plant dry weight of 13-day-old seedlings during a 2-year period increased by 3.71%for Wuyunjing 24 and by 3.78%for 6 Liangyou 9368 at the MS.Moreover,as seedling age increased,the leaf area index and photosynthetic potential decreased for both cultivars,and the photosynthetic rate markedly decreased at the heading stage(HS).With the exception of that of Wuyunjing 24 from the jointing stage to the HS in 2014,the crop growth rate was higher for young seedlings than for old seedlings.Grain yield significantly decreased with seedling age,but no significant difference was detected between the 13-and 20-day-old seedlings for either cultivar.Therefore,equilibrious and high biological yield formation,vigorous growth in the late stages,and high photosynthetic production capacity are important characteristics and causes of the efficient and sustainable output of photosynthetic systems and for achieving high yield formation in young transplanted seedlings(13-20-day-old).展开更多
The seedlings of 6 rice varieties were treated with Cd concentrations of 25,50 and 100μmol/L for 5 d.The results showed that the longest root length,seedling height,fresh weight per plant and dry weight per plant of ...The seedlings of 6 rice varieties were treated with Cd concentrations of 25,50 and 100μmol/L for 5 d.The results showed that the longest root length,seedling height,fresh weight per plant and dry weight per plant of 6 rice varieties were significantly inhibited.The inhibiting effects showed a trend as the following:the longest root length>fresh weight per plant>dry weight per plant>seedling height,and the inhibiting effect increased with the increase of Cd stress concentration.It is more economical and reasonable to use 50μmol/L Cd stress concentration to identify Cd tolerance of rice seedlings.And it is more scienti fic to evaluate the Cd tolerance of rice seedling comprehensively by using the 4 indexes of longest root length,fresh weight per plant,dry weight per plant and seedling height.展开更多
Rice with polyembryony characterized bytwin seedlings is a good genetic tool for apomixisresearch.A study on the effect of five plantgrowth regulators(IAA,KT,GA,2,4-D and6-BAP)on rate of twin seedlings in 3 rice varie...Rice with polyembryony characterized bytwin seedlings is a good genetic tool for apomixisresearch.A study on the effect of five plantgrowth regulators(IAA,KT,GA,2,4-D and6-BAP)on rate of twin seedlings in 3 rice varie.ties with polyembryony(Shuang 3,Shuang 13and Lu 52)was conducted.The results showed展开更多
Using early indica rice Zhongzao 39 and japonica rice Xiushui 134 as the experimental materials, the effects of 4 temperature levels after transplantation on turning green, leaf growth, tillering, dry matter accumulat...Using early indica rice Zhongzao 39 and japonica rice Xiushui 134 as the experimental materials, the effects of 4 temperature levels after transplantation on turning green, leaf growth, tillering, dry matter accumulation and nutrition absorption were studied using a greenhouse and an artificial climate chest. We found that (1) tillering did not occur and growth of one new leaf took over 15 days at 16℃. New leaf growth took over 10 days and tillering occurred after 15 days of transplanta- tion at 19 ℃. Tillering appeared 10 days after planting and new leaf growth took 5 days between 22 and 25 ℃. This showed that low temperature impeded the turn- ing green of rice seedlings and the temperature must reach above 19 ℃ to ensure timely appearance of new leaf, tillering and turning green in rice seedlings. (2) When the temperature was 10 increased, both 100-seedling dry weight and nitrogen absorption increased. At temperatures lower 11 than 19 ℃, both dry weight incre- ment and nitrogen absorption were low. Nitrogen absorption of all organs increased obviously between 19 and 22 ℃. Thus, 19 ℃ was found to be an minimum tem- perature for plant organs to absorb nutrients. (3)Tiller of seedling cultivated on dry- land soil and substrate grew quickly and tillering occurred about 7 days after trans- planting. After that, the growth rate was about 0.18 tiller per day. For seedlings cultivated in slurry, tillering occurred 10 days after transplanting and the tillers grew at a rate of 0.16 tiller per day. However, substrate choice during the seedling rais- ing stage had no significant influence on leaf age. (4) Japonica rice exhibited stronger resistance to low temperature than indica rice. Tillering began at about the same time after transplanting in both rice varieties, but japonica rice exhibited higher tillering speed than early indica rice. The speed of leaf growth was higher in japoni- ca rice before transplantation but higher in indica rice after transplantation.展开更多
[Objective] With a rice variety "Long Rice 11" as a test cultivar,an experiment of raising rice seedlings with the new substrata prepared from biochar,and maize stalks,rice husks,organic fertilizer,turf,zeolite,fine...[Objective] With a rice variety "Long Rice 11" as a test cultivar,an experiment of raising rice seedlings with the new substrata prepared from biochar,and maize stalks,rice husks,organic fertilizer,turf,zeolite,fine river sand and arable layer soil by mixing according to certain volume proportions was caried out,in order to investigate the physical and chemical properties of different organic-material seedling-raising substrata and the effects of these substrata on seedling growth.[Method] The experiment raised seedlings in greenhouses and adopted randomized block arrangement.[Result] The substratum of biochar mixed with maize stalks and rice husks could increase the maximum water-holding capacity of the substratum,reduce the volume weight of the substratum and improve the buffering effect of the substratum.It also had great effects on the contents of alkali-hydrolyzale nitrogen and rapidly available potassium in the substratum,and could improve the root number and substantial degree of rice seedlings.[Conclusion] Biochar with maize stalks and rice husks(the treament HC) is the optimal substratum in this study.展开更多
This study was conducted to investigate the effects of bast fiber seedling film on rice seedling growth. In this study, indica cultivar 'Xiangwanxian 13' and indica hybrid cuitivar 'Y Liangyou 1' were used to inve...This study was conducted to investigate the effects of bast fiber seedling film on rice seedling growth. In this study, indica cultivar 'Xiangwanxian 13' and indica hybrid cuitivar 'Y Liangyou 1' were used to investigate the changes of aboveground part growth and root respiration enzyme activities of rice seedlings af- ter the application of bast fiber seedling film. The results showed that, compared with the CK, the accumulation of shoot biomass, chlorophyll content, root bleeding intensity, soluble sugar content, and nitrate nitrogen content were significantly im- proved with the application of bast fiber seedling film. In addition, the activities of malate dehydrogenas (MDH) and alcohol dehydrogenas (ADH) were higher in rice seedling roots raised with bast fiber seedling film, while there was no significant change in lactate dehydrogenase (LDH) after the application of bast fiber seedling film. However, there were no significant differences in these indexes between the two cultivars. All the results indicated that the growth and development of rice seedlings could be improved with the application of bast fiber seedling film.展开更多
The genetic pathways of rice seedling growth have a major impact on seedling emergence from soil and development.In this study,we identified a new bHLH transcription factor,BEAR1,from rice RNAi mutant library.Both the...The genetic pathways of rice seedling growth have a major impact on seedling emergence from soil and development.In this study,we identified a new bHLH transcription factor,BEAR1,from rice RNAi mutant library.Both the BEAR1-RNAi and bear1 CRISPR mutants had accelerated seedling growth.Histological section of leaves showed accelerated development of lacuna and vascular bundles in bear1 mutant.GUS staining revealed that BEAR1 was highly expressed in coleoptiles and leaves at seedling stage.Expression analysis of gibberellin(GA)biosynthesis and metabolic genes and content determination of active GAs indicated that the expression of GA biosynthesis genes,especially OsKS4 and OsCPS2,were upregulated and the GAs content were significantly increased in bear1,which correlated with the seedling phenotype of bear1 mutant.Molecular and biochemical assays revealed that BEAR1 directly binds to the promoter of OsKS4,thereby repressing its expression.Haplotypes analysis showed clear differentiation in indica and japonica rice varieties,and a strong correlation with seedling height.These findings provide novel insights into the regulation of seedling growth in rice.展开更多
This study was conducted to isolate and characterize the plant growth-promoting potential of fungal endophytes from the roots of Diket red,a traditional rice plant from the Cordillera,Northern Luzon.Eighteen morphospe...This study was conducted to isolate and characterize the plant growth-promoting potential of fungal endophytes from the roots of Diket red,a traditional rice plant from the Cordillera,Northern Luzon.Eighteen morphospecies of filamentous endophytes were isolated of which twelve isolates were successfully identified to the species level.These isolates were identified as Aspergillus versicolor,Aspergillus sp.,Chaetosphaeria sp.,Cladosporium cladosporioides,Hypocrea lixii,Microascus murinus,and Trichoderma harzianum.The identified twelve isolates were selected to screen in vitro for their plant growth-promoting characteristics,and evaluated in vivo for their beneficial effects on seedling vigor and early seedling growth.Isolate PPL14 produced the highest IAA at 55.5μg ml^(-1)and M.murinus PPL10 produced the highest amount of IAA at 3.73μg IAA mg^(-1)dry mycelia wt.The seedling vigor assay and in vivo plant growth promotion bioassay indicated overall positive effects of culture filtrate(CF)application of the endophyte isolates.Rice seeds and seedlings grown in aseptic condition and treated with endophyte CFs displayed significantly enhanced levels of germination,seedling vigor,shoot,root,and total plant growth,and biomass compared to non-treated control.Other plant growth-promoting characteristics were also studied including phosphate solubilization,siderophore production,ammonia production,and catalase activity.This study supports the potential use of fungal endophytes as bio-inoculants for plant growth promotion and enhancement of nutrient assimilation of agriculturally important crops.展开更多
The effects of soil moisture content, pot seedling buried depth, and pot seedling height on the survival rate and growth of dry planted seedlings were researched by field experiment. The results showed that the seedli...The effects of soil moisture content, pot seedling buried depth, and pot seedling height on the survival rate and growth of dry planted seedlings were researched by field experiment. The results showed that the seedling survival rate and growth were enhanced with the increase of soil moisture content after pot seedling transplanting, but there was no significant difference in the survival rate and growth of seedlings with 85% and 100% of soil moisture content. Pot seedling buried depth was one of the important factors affecting the survival rate, and when the pot seedlings were buried 2 cm deep, the seedlings showed high survival rate and excellent growth. Under the same condition of soil moisture content and buried depth, seedlings with pot seedling height of 15 cm had the best survival rate and growth. Therefore, the optimized conditions for dry-cultivated rice seedling planting were soil moisture content of 85%, pot seedling buried depth of 2 cm, and pot seedling height of 15 cm.展开更多
Phosphorus(P)is an essential element for plant growth but is often limiting in ecosystems;therefore,improving the P fertilizer use efficiency is important.Biochar and arbuscular mycorrhizal fungi(AMF)may enhance P cyc...Phosphorus(P)is an essential element for plant growth but is often limiting in ecosystems;therefore,improving the P fertilizer use efficiency is important.Biochar and arbuscular mycorrhizal fungi(AMF)may enhance P cycling in paddy soils that contain high content of total P but low content of available P(AP).In this study,the effects of biochar addition and Rhizophagus irregularis inoculation on the organic and inorganic P contents and phosphatase activities in paddy soils,rice seedling growth,and AMF colonization were investigated.Compared with no biochar addition,biochar addition enhanced the percentage of spore germination at day 7,hyphal length,most probable number,and mycorrhizal colonization rate of R.irregularis by 32%,662%,70%,and 28%on average,respectively.Biochar and R.irregularis altered soil P cycling and availability.Biochar and R.irregularis,either individually or in combination,increased soil AP content by 2%–48%.Rice seedlings treated with biochar and R.irregularis produced greater biomass,improved root morphology,and increased nutrient uptake compared with those of the control without biochar and R.irregularis.The results suggest that combined application of biochar and R.irregularis is beneficial to rice cultivation in paddy soils with high content of total P but low content of AP.展开更多
文摘The process of rice(Oryza sativa L.)seedling cultivation is often subjected to adverse environmental stress.Biostimulants regulate the robust growth of rice seedlings and play a crucial role in promoting the green and ecological development of agriculture.In this study,1.0 and 2.0 g•m^(-2) of the biostimulant were applied to soil in rice seedbeds.Growth indicators of rice,antioxidant enzyme activities and soil physicochemical characteristics were assessed at the 2.5-leaf and 4-leaf stages of rice.The results indicated that applying 2.0 g•m^(-2) of the biostimulant at both the 2.5-leaf and 4-leaf stages had the most significant promoting effect on rice growth.At the 2.5-leaf and 4-leaf stages,the number of fibrous roots increased by 23.43%and 22.25%,stem base width increased by 19.05%and 19.58%,above ground dry weight increased by 18.09%and 16.47%,root dry weight increased by 19.67%and 18.28%,leaf peroxidase(POD)activity increased by 34.44%and 42.94%,superoxide dismutase(SOD)activity increased by 37.24%and 56.79%,malondialdehyde(MDA)content decreased by 18.60%and 27.67%,and chlorophyll content increased significantly by 28.31%and 34.24%,respectively.At the 4-leaf stage of rice,urease,phosphatase and cellulase activities in the seedbed soil increased by 42.13%,25.96%and 33.59%,respectively,while soil alkaline nitrogen,available phosphorus and available potassium content decreased by 19.76%,19.02%and 17.88%,respectively.The application of biostimulants played a crucial role in promoting the growth of rice seedlings and enhancing soil nutrient absorption.
基金Supported by Loudi City Science and Technology Project(2014-1)State Key Laboratory of Hybrid RiceKey Laboratory of Pesticide Harmless Application of Hunan Province~~
文摘The research used bioassay method to explore seed tolerance of Cd in terms of seed germination and seedling growth. Specifically, 12 rice varieties of three types were investigated under Cd stress, covering germination rate, shooting index, vigor index, seedling and shoot lengths, and fresh weights. The results showed that as Cd concentration grew, seed germination and seedling growth kept increasingly inhibited; germination index, vigor index, root length, shoot length and fresh weights went down; inhibition effects on seed germination and seedling growth were conventional rice〉two-line hybrid rice〉three-line hybrid ,rice. Shanyou 9588, Tianyou 998, Chuanxiang 8, and Y Liangyou 1 performed ~rongly in Cd tolerance; C Liangyou 651, Nei 5 You 263, Xiangwanxian 16, Huanghuazhan, Xiangwanxian 13, and Zhunliangyou 527 performed poorly in Cd tolerance, and Cd tolerance of rest treatments were moderate.
基金Supported by National Science and Technology Innovation Program for High-yielding and High-efficiency Grain Crops(2013BAD07B13-02)Special Fund for Agro-scientific Research in the Public Interest(201303102)~~
文摘The effects of farming method, rice variety and seedling-raising method were studied on the population growth quality and grain yield of the mechanized transplanting rice by way of three-factor split plot design. The results showed that the no-tillage mechanical transplanting treatment was poorer than the conventional mechanical transplanting treatment in transplanting quality in general, but the former was similar to the latter in population growth quality. In regard to grain yield, the no-tillage mechanical transplanting treatment was lower than the conventional me- chanical transplanting treatment. Raising both pot-mat seedling and plastic-tray seedling did not make much difference in population growth and grain yield of mechanized transplanting rice, so both of the seedling-raising methods can be adopted according to practical conditions.
基金Funding was provided by the National Key Research and Development Program of China(2017YFD0301200,2018YFD0300803,2015BAD01B03)the Jiangsu Key Research and Development Program,China(BE2017369)the Jiangsu Agricultural Science and Technology Innovation Fund,China(JASTIF,CX(18)1002).
文摘Understanding the characteristics of rice productivity is of great importance for achieving high yield formation.However,such traits have not yet been studied for different ages of hydroponically grown long-mat rice seedlings(HLMS),which constitutes a new method of seedling cultivation.Field experiments were conducted to evaluate the effects of seedling age on the growth stage,photosynthesis characteristics,dry matter production,and yield of HLMS.A conventional japonica rice cultivar(Wuyunjing 24)and an indica hybrid rice cultivar(6 Liangyou 9368)were used as test materials.The results showed that the whole phase was shortened by 13-15 days for young seedlings(13-day-old)compared with old seedlings(27-day-old),which occurred because the growth process accelerated with the transplantation of young seedlings.As seedling age increased,the dry matter weight of stems of individual plants and of the population increased at the transplanting stage but decreased at the maturity stage(MS).Compared with that of 27-day-old seedlings,the average ratio of panicle weight to total plant dry weight of 13-day-old seedlings during a 2-year period increased by 3.71%for Wuyunjing 24 and by 3.78%for 6 Liangyou 9368 at the MS.Moreover,as seedling age increased,the leaf area index and photosynthetic potential decreased for both cultivars,and the photosynthetic rate markedly decreased at the heading stage(HS).With the exception of that of Wuyunjing 24 from the jointing stage to the HS in 2014,the crop growth rate was higher for young seedlings than for old seedlings.Grain yield significantly decreased with seedling age,but no significant difference was detected between the 13-and 20-day-old seedlings for either cultivar.Therefore,equilibrious and high biological yield formation,vigorous growth in the late stages,and high photosynthetic production capacity are important characteristics and causes of the efficient and sustainable output of photosynthetic systems and for achieving high yield formation in young transplanted seedlings(13-20-day-old).
基金Supported by Changsha Science and Technology Project(kq180103)Hunan Agricultural Science and Technology Innovation Fund Project(2017XC10)Hunan Agricultural Science and Technology Innovation Fund Project(2017JC12)~~
文摘The seedlings of 6 rice varieties were treated with Cd concentrations of 25,50 and 100μmol/L for 5 d.The results showed that the longest root length,seedling height,fresh weight per plant and dry weight per plant of 6 rice varieties were significantly inhibited.The inhibiting effects showed a trend as the following:the longest root length>fresh weight per plant>dry weight per plant>seedling height,and the inhibiting effect increased with the increase of Cd stress concentration.It is more economical and reasonable to use 50μmol/L Cd stress concentration to identify Cd tolerance of rice seedlings.And it is more scienti fic to evaluate the Cd tolerance of rice seedling comprehensively by using the 4 indexes of longest root length,fresh weight per plant,dry weight per plant and seedling height.
文摘Rice with polyembryony characterized bytwin seedlings is a good genetic tool for apomixisresearch.A study on the effect of five plantgrowth regulators(IAA,KT,GA,2,4-D and6-BAP)on rate of twin seedlings in 3 rice varie.ties with polyembryony(Shuang 3,Shuang 13and Lu 52)was conducted.The results showed
基金Supported by Special Scientific Research Funds for Commonweal Section(Agriculture)(201203029,201003016)China Rice Industry System Project(2011-2015)Special Funding for Basic Scientific Research and Zhejiang Provincial Natural Science Foundation(LY13C130004)~~
文摘Using early indica rice Zhongzao 39 and japonica rice Xiushui 134 as the experimental materials, the effects of 4 temperature levels after transplantation on turning green, leaf growth, tillering, dry matter accumulation and nutrition absorption were studied using a greenhouse and an artificial climate chest. We found that (1) tillering did not occur and growth of one new leaf took over 15 days at 16℃. New leaf growth took over 10 days and tillering occurred after 15 days of transplanta- tion at 19 ℃. Tillering appeared 10 days after planting and new leaf growth took 5 days between 22 and 25 ℃. This showed that low temperature impeded the turn- ing green of rice seedlings and the temperature must reach above 19 ℃ to ensure timely appearance of new leaf, tillering and turning green in rice seedlings. (2) When the temperature was 10 increased, both 100-seedling dry weight and nitrogen absorption increased. At temperatures lower 11 than 19 ℃, both dry weight incre- ment and nitrogen absorption were low. Nitrogen absorption of all organs increased obviously between 19 and 22 ℃. Thus, 19 ℃ was found to be an minimum tem- perature for plant organs to absorb nutrients. (3)Tiller of seedling cultivated on dry- land soil and substrate grew quickly and tillering occurred about 7 days after trans- planting. After that, the growth rate was about 0.18 tiller per day. For seedlings cultivated in slurry, tillering occurred 10 days after transplanting and the tillers grew at a rate of 0.16 tiller per day. However, substrate choice during the seedling rais- ing stage had no significant influence on leaf age. (4) Japonica rice exhibited stronger resistance to low temperature than indica rice. Tillering began at about the same time after transplanting in both rice varieties, but japonica rice exhibited higher tillering speed than early indica rice. The speed of leaf growth was higher in japoni- ca rice before transplantation but higher in indica rice after transplantation.
文摘[Objective] With a rice variety "Long Rice 11" as a test cultivar,an experiment of raising rice seedlings with the new substrata prepared from biochar,and maize stalks,rice husks,organic fertilizer,turf,zeolite,fine river sand and arable layer soil by mixing according to certain volume proportions was caried out,in order to investigate the physical and chemical properties of different organic-material seedling-raising substrata and the effects of these substrata on seedling growth.[Method] The experiment raised seedlings in greenhouses and adopted randomized block arrangement.[Result] The substratum of biochar mixed with maize stalks and rice husks could increase the maximum water-holding capacity of the substratum,reduce the volume weight of the substratum and improve the buffering effect of the substratum.It also had great effects on the contents of alkali-hydrolyzale nitrogen and rapidly available potassium in the substratum,and could improve the root number and substantial degree of rice seedlings.[Conclusion] Biochar with maize stalks and rice husks(the treament HC) is the optimal substratum in this study.
基金Supported by Scientific and Technological Innovation Project of Chinese Academy of Agricultural Sciences(ASTIP-IBFC07)Production Post of Degradable Bast Fiber Mulching Film of National Agro-Industry Technology Research System for Bast and Leaf Fiber Crops(CARS-19-E23)~~
文摘This study was conducted to investigate the effects of bast fiber seedling film on rice seedling growth. In this study, indica cultivar 'Xiangwanxian 13' and indica hybrid cuitivar 'Y Liangyou 1' were used to investigate the changes of aboveground part growth and root respiration enzyme activities of rice seedlings af- ter the application of bast fiber seedling film. The results showed that, compared with the CK, the accumulation of shoot biomass, chlorophyll content, root bleeding intensity, soluble sugar content, and nitrate nitrogen content were significantly im- proved with the application of bast fiber seedling film. In addition, the activities of malate dehydrogenas (MDH) and alcohol dehydrogenas (ADH) were higher in rice seedling roots raised with bast fiber seedling film, while there was no significant change in lactate dehydrogenase (LDH) after the application of bast fiber seedling film. However, there were no significant differences in these indexes between the two cultivars. All the results indicated that the growth and development of rice seedlings could be improved with the application of bast fiber seedling film.
基金supported by the National Natural Science Foundation of China(32071932)Scientific Research Foundation for Scholars of HZNU(2019QDL015)the Natural Science Foundation of Zhejiang province(LQ22C130001)。
文摘The genetic pathways of rice seedling growth have a major impact on seedling emergence from soil and development.In this study,we identified a new bHLH transcription factor,BEAR1,from rice RNAi mutant library.Both the BEAR1-RNAi and bear1 CRISPR mutants had accelerated seedling growth.Histological section of leaves showed accelerated development of lacuna and vascular bundles in bear1 mutant.GUS staining revealed that BEAR1 was highly expressed in coleoptiles and leaves at seedling stage.Expression analysis of gibberellin(GA)biosynthesis and metabolic genes and content determination of active GAs indicated that the expression of GA biosynthesis genes,especially OsKS4 and OsCPS2,were upregulated and the GAs content were significantly increased in bear1,which correlated with the seedling phenotype of bear1 mutant.Molecular and biochemical assays revealed that BEAR1 directly binds to the promoter of OsKS4,thereby repressing its expression.Haplotypes analysis showed clear differentiation in indica and japonica rice varieties,and a strong correlation with seedling height.These findings provide novel insights into the regulation of seedling growth in rice.
基金This project is supported by a Junior Thesis Fellowship Grant awarded to the authors by the Philippine Rice Research Institute(PhilRice).
文摘This study was conducted to isolate and characterize the plant growth-promoting potential of fungal endophytes from the roots of Diket red,a traditional rice plant from the Cordillera,Northern Luzon.Eighteen morphospecies of filamentous endophytes were isolated of which twelve isolates were successfully identified to the species level.These isolates were identified as Aspergillus versicolor,Aspergillus sp.,Chaetosphaeria sp.,Cladosporium cladosporioides,Hypocrea lixii,Microascus murinus,and Trichoderma harzianum.The identified twelve isolates were selected to screen in vitro for their plant growth-promoting characteristics,and evaluated in vivo for their beneficial effects on seedling vigor and early seedling growth.Isolate PPL14 produced the highest IAA at 55.5μg ml^(-1)and M.murinus PPL10 produced the highest amount of IAA at 3.73μg IAA mg^(-1)dry mycelia wt.The seedling vigor assay and in vivo plant growth promotion bioassay indicated overall positive effects of culture filtrate(CF)application of the endophyte isolates.Rice seeds and seedlings grown in aseptic condition and treated with endophyte CFs displayed significantly enhanced levels of germination,seedling vigor,shoot,root,and total plant growth,and biomass compared to non-treated control.Other plant growth-promoting characteristics were also studied including phosphate solubilization,siderophore production,ammonia production,and catalase activity.This study supports the potential use of fungal endophytes as bio-inoculants for plant growth promotion and enhancement of nutrient assimilation of agriculturally important crops.
基金Supported by the Key Research and Development Project of Shandong Province(2016GNC111005&2015GNC111019)
文摘The effects of soil moisture content, pot seedling buried depth, and pot seedling height on the survival rate and growth of dry planted seedlings were researched by field experiment. The results showed that the seedling survival rate and growth were enhanced with the increase of soil moisture content after pot seedling transplanting, but there was no significant difference in the survival rate and growth of seedlings with 85% and 100% of soil moisture content. Pot seedling buried depth was one of the important factors affecting the survival rate, and when the pot seedlings were buried 2 cm deep, the seedlings showed high survival rate and excellent growth. Under the same condition of soil moisture content and buried depth, seedlings with pot seedling height of 15 cm had the best survival rate and growth. Therefore, the optimized conditions for dry-cultivated rice seedling planting were soil moisture content of 85%, pot seedling buried depth of 2 cm, and pot seedling height of 15 cm.
基金financially supported by the Guiding Fund of the Central Government for Local Science and Technology Development,China(No.2023JH6/100100056)the Science and Technology Plan Project of Shenyang,China(No.22317-2-08)the Earmarked Fund for Modern Agroindustry Technology Research System,China(No.CARS-01-52)。
文摘Phosphorus(P)is an essential element for plant growth but is often limiting in ecosystems;therefore,improving the P fertilizer use efficiency is important.Biochar and arbuscular mycorrhizal fungi(AMF)may enhance P cycling in paddy soils that contain high content of total P but low content of available P(AP).In this study,the effects of biochar addition and Rhizophagus irregularis inoculation on the organic and inorganic P contents and phosphatase activities in paddy soils,rice seedling growth,and AMF colonization were investigated.Compared with no biochar addition,biochar addition enhanced the percentage of spore germination at day 7,hyphal length,most probable number,and mycorrhizal colonization rate of R.irregularis by 32%,662%,70%,and 28%on average,respectively.Biochar and R.irregularis altered soil P cycling and availability.Biochar and R.irregularis,either individually or in combination,increased soil AP content by 2%–48%.Rice seedlings treated with biochar and R.irregularis produced greater biomass,improved root morphology,and increased nutrient uptake compared with those of the control without biochar and R.irregularis.The results suggest that combined application of biochar and R.irregularis is beneficial to rice cultivation in paddy soils with high content of total P but low content of AP.