Aim To study the effect and mechanism of Chlorogenic acid in allergic rhinitis （AR） mice. MethodsAR was induced in BALB/c mice according to a published method with ovalbumin （OVA）. The AR model was de- veloped by ...Aim To study the effect and mechanism of Chlorogenic acid in allergic rhinitis （AR） mice. MethodsAR was induced in BALB/c mice according to a published method with ovalbumin （OVA）. The AR model was de- veloped by intraperitoneal injection of adjuvant suspension on every other day for 7 times. Adjuvant suspension in- clude 50 μg of OVA and 2rag of AI（OH） s, which were combined in 2ml of normal saline （NS）. Then, the mice received a nasal drip of 10% OVA daily for 10days, and in normal control were dripped NS. After administration with 50 mg· kg^-1, 100 mg · kg^-1 and 200 mg · kg^-1 of Chlorogenic acid for 30min, The mice were attacked lo- cally for 10 days. We observed behavior analysis, evaluated the weight of nosal draingage, related the index of thy- mus and spleen, determined the effect of Chlorogenic acid on the contents of cytokines, IgE and histamine by ELISA in AR mice and analyzed the mRNA expression of inflammatory cytokines （IL-4, IL-5, IL-10, IFN-y） in the nasal mucosa tissues samples by RT-PCR. Results Chlorogenic acid decreased the number of nose-scratching events in 10min, the weight of nosal draingage, the index of spleen, the content of IL-4 in nasal lavage fluid（NLF） and the contents of cytokines, IgE and histamine in serum; while increased the index of thymus, the content of IFN-γ in NLF, the contents of IFN-γ in serum and the rate of IFN-γ/IL-4 ; The data showed a statistically signifi- cant up-regulation of IFN-γ mRNA expressions and down-regulation of IL-4mRNA, IL-5mRNA, IL-10mRNA ex- pressions after adiminstration of Chlorogenic acid. Conclusion Chlorogenic acid might exert the effect in AR mice through regulating the balance of Thl and Th2.展开更多
As probiotics in the gut, Lactobacilli are believed to play important roles in the development and maintenance of both the mucosal and systemic immune system of the host. This study was aimed to investigate the immuno...As probiotics in the gut, Lactobacilli are believed to play important roles in the development and maintenance of both the mucosal and systemic immune system of the host. This study was aimed to investigate the immunomodulatory function of candiate lactobacilli on T cells. Lactobacilli were isolated from healthy human feces and the microbiological characteristics were identified by API 50 CHL and randomly amplified polymorphic DNA （RAPD） assays. Anti-CD3 antibody activated peripheral blood mononuclear cells （PBMCs） were treated by viable, heat-killed lactobacilli and genomic DNA of lactobacilli, and cytokine profiles were tested by ELISA. Isolated lactobacilli C44 and C48 were identified as L. acidophilus and L. paracacei, which have properties of acid and bile tolerance and inhibitor effects on pathogens. Viable and heat-killed C44 and C48 induced low levels of proinflammatory cytokines （TNF-a, IL-6 and IL-8） and high levels of IFN-y and IL-12p70 in PBMCs. In anti- CD3 antibody activated PBMCs, viable and heat-killed C44 increased Th2 cytokine levels （IL-5, IL-6 and IL-10）, and simultaneously enhanced Thl responses by inducing IFN-y and IL-12p70 production. Different from that of lactabacillus strains, their genomic DNA induced low levels of IL-12p70, IFN-y and proinflammatory cytokines in PBMCs with or without anti-CD3 antibody activation. These results provided in vitro evidence that the genomic DNA of strains of C44 and C48, especially C44, induced weaker inflammation, and may be potentially applied for treating allergic diseases.展开更多
文摘Aim To study the effect and mechanism of Chlorogenic acid in allergic rhinitis （AR） mice. MethodsAR was induced in BALB/c mice according to a published method with ovalbumin （OVA）. The AR model was de- veloped by intraperitoneal injection of adjuvant suspension on every other day for 7 times. Adjuvant suspension in- clude 50 μg of OVA and 2rag of AI（OH） s, which were combined in 2ml of normal saline （NS）. Then, the mice received a nasal drip of 10% OVA daily for 10days, and in normal control were dripped NS. After administration with 50 mg· kg^-1, 100 mg · kg^-1 and 200 mg · kg^-1 of Chlorogenic acid for 30min, The mice were attacked lo- cally for 10 days. We observed behavior analysis, evaluated the weight of nosal draingage, related the index of thy- mus and spleen, determined the effect of Chlorogenic acid on the contents of cytokines, IgE and histamine by ELISA in AR mice and analyzed the mRNA expression of inflammatory cytokines （IL-4, IL-5, IL-10, IFN-y） in the nasal mucosa tissues samples by RT-PCR. Results Chlorogenic acid decreased the number of nose-scratching events in 10min, the weight of nosal draingage, the index of spleen, the content of IL-4 in nasal lavage fluid（NLF） and the contents of cytokines, IgE and histamine in serum; while increased the index of thymus, the content of IFN-γ in NLF, the contents of IFN-γ in serum and the rate of IFN-γ/IL-4 ; The data showed a statistically signifi- cant up-regulation of IFN-γ mRNA expressions and down-regulation of IL-4mRNA, IL-5mRNA, IL-10mRNA ex- pressions after adiminstration of Chlorogenic acid. Conclusion Chlorogenic acid might exert the effect in AR mice through regulating the balance of Thl and Th2.
基金supported by Global Watch International Secondment ChinaUK Department of Trade and Industry
文摘As probiotics in the gut, Lactobacilli are believed to play important roles in the development and maintenance of both the mucosal and systemic immune system of the host. This study was aimed to investigate the immunomodulatory function of candiate lactobacilli on T cells. Lactobacilli were isolated from healthy human feces and the microbiological characteristics were identified by API 50 CHL and randomly amplified polymorphic DNA （RAPD） assays. Anti-CD3 antibody activated peripheral blood mononuclear cells （PBMCs） were treated by viable, heat-killed lactobacilli and genomic DNA of lactobacilli, and cytokine profiles were tested by ELISA. Isolated lactobacilli C44 and C48 were identified as L. acidophilus and L. paracacei, which have properties of acid and bile tolerance and inhibitor effects on pathogens. Viable and heat-killed C44 and C48 induced low levels of proinflammatory cytokines （TNF-a, IL-6 and IL-8） and high levels of IFN-y and IL-12p70 in PBMCs. In anti- CD3 antibody activated PBMCs, viable and heat-killed C44 increased Th2 cytokine levels （IL-5, IL-6 and IL-10）, and simultaneously enhanced Thl responses by inducing IFN-y and IL-12p70 production. Different from that of lactabacillus strains, their genomic DNA induced low levels of IL-12p70, IFN-y and proinflammatory cytokines in PBMCs with or without anti-CD3 antibody activation. These results provided in vitro evidence that the genomic DNA of strains of C44 and C48, especially C44, induced weaker inflammation, and may be potentially applied for treating allergic diseases.
