Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling

Three-dimensional(3D)culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.In cancer and stem cell research,the natural cell characteristics and architectures are closely mimicked by the 3D cell models.Thus,the 3D cell cultures are promising and suitable systems for various proposes,ranging from disease modeling to drug target identification as well as potential therapeutic substances that may transform our lives.This review provides a comprehensive compendium of recent advancements in culturing cells,in particular cancer and stem cells,using 3D culture techniques.The major approaches highlighted here include cell spheroids,hydrogel embedding,bioreactors,scaffolds,and bioprinting.In addition,the progress of employing 3D cell culture systems as a platform for cancer and stem cell research was addressed,and the prominent studies of 3D cell culture systems were discussed.

A Coordinate-Free Approach to the Design of Generalized Griffis-Duffy Platforms

Architectural singularity belongs to the Type II singularity,in which a parallel manipulator(PM)gains one or more degrees of freedom and becomes uncontrollable.PMs remaining permanently in a singularity are beneficial for linearto-rotary motion conversion.Griffis-Duffy(GD)platform is a mobile structure admitting a Bricard motion.In this paper,we present a coordinate-free approach to the design of generalized GD platforms,which consists in determining the shape and attachment of both the moving platform and the fixed base.The generalized GD platform is treated as a combination of six coaxial single-loop mechanisms under the same constraints.Owing to the inversion,hidden in the geometric structure of these single-loop mechanisms,the mapping from a line to a circle establishes the geometric transformation between the fixed base and the moving platform based on the center of inversion,and describes the shape and attachment of the generalized GD platform.Moreover,the center of inversion not only identifies the location of rotation axis,but also affects the shape of the platform mechanism.A graphical construction of generalized GD platforms using inversion,proposed in the paper,provides geometrically feasible solutions of the manipulator design for the requirement of the location of rotation axis.

Design Method for Optimizing the Interactive Interface of Live Broadcasting Platform for the Elderly Users

In the era of network live broadcasting for everyone,the development of live broadcasting platforms is also more intelligent and diversified.However,in the face of a large group of elderly users,the interface interaction design mode used is still mainly based on the interaction mode for young groups,and is not designed for elderly users.Therefore,a design method for optimizing the interaction interface of live broadcasting platform for elderly users was proposed in this study.Firstly,the case study method and Delphi expert survey method were used to determine the design needs of elderly users and the design mode was analysed.Secondly,the orthogonal design principle was used to design a test sample of the interactive interface of live broadcasting platform applicable for the elderly users,and then a user evaluation system was established to calculate the weights of the design elements using hierarchical analysis,and then the predictive relationship between the design mode of the interactive interface of live broadcasting platform and the elderly users was established by Quantitative Theory I.Finally,Genetic Algorithm was applied to generate the optimized design scheme.The results showed that the design method based on the Genetic Algorithm and the combination of Quantitative Theory can scientifically and effectively optimize the design of the interactive interface of the live broadcasting platform for the elderly users,and improve the experience of the elderly users.

A combination of digital design and three-dimensional printing to assist treatment of thoracolumbar compression fractures using percutaneous kyphoplasty

Objective:To evaluate the clinical efficacy of the preoperative digita1 design combined with three dimensional(3D)printing models to assist percutaneous kyphoplasty(PKP)treatment for thoracolumbar compression frac tures.Methods:From January 2018 to August 2020,we obtained data of 99 patients diagnosed thoracolumbar compression fractures.These patients were divided into control group(n=50)underwent traditional PKP surgery,and observation group(n=49)underwent preoperative digital design combined with 3D printing model assisted PKP treatment.The clinical efficacy was evaluated with five parameters,including operation time,number of intraoperative radiographs,visual analogue scale(VAS)score,Cobb Angle change,and high compression rate of injured vertebrae.Results:There were statistically significant differences of operation time and number of intraoperative radio graphs between the two groups(P<0.05).For VAS score,Cobb Angle change and vertebral height compression rate,all of these three parameters were significantly improved when the patients accepted surgery teatment in two groups(P<0.05).However,there were no significant differences between control group and observation group for these three parameters either before or after surgery(P>0.05).Conclusions:Through the design of preoperative surgical guide plate and the application of 3D printing model to guide the operation,the precise design of preoperative surgical puncture site and puncture Angle of the injured vertebra was realized,the number of intraoperative radiographs was reduced,the operation time was shortened and the operation efficiency was improved.

The Design of a Three-Dimensional Physical Modeling System for Real-Time Groundwater Flows

In the past decades,physical modeling has been widely used in hydrogeology for teaching,studying and exhibition purposes.Most of these models are used to illustrate hydrogeological profiles,but few can depict three-dimensional groundwater flows,making it impossible to validate groundwater flows simulated by numerical methods with physical modeling.

A Win-Win Undergraduate Graduation Design Model for Students, Teachers, and Training Platforms

Undergraduate graduation design is an important link in the process of undergraduate training,and university platforms have invested a lot of manpower and material resources for this purpose.However,while carrying out student training,it has gradually become the consensus of most university platforms to achieve a win-win situation for teachers and training platforms in order to achieve optimization of resource allocation and motivation of student training.After discussion and practice,this paper proposes a set of undergraduate graduation design training concepts and training modes with a win-win situation for students,teachers,and training platforms.

Structural Design and Optimization of Comb-type Electric Bicycle Three-dimensional Parking Garage

With the reduction of urban land, the three-dimensional garage is increasingly built with its advantages of saving land. But the current three-dimensional garage is built for the car. It is hardly stereo parking garage for electric bicycles. This paper designed a hollow tower electric bicycle stereo parking garage with fork comb structure, based on the analysis of the characteristics of electric bicycles and the characteristics of existing three-dimensional garages. A fixed comb is mounted on the garage frame. The movable comb is mounted on the middle lift mechanism of the garage. The access of the vehicle is achieved by the exchange of the comb. The key comb structure was modeled using SolidWorks software and the stress distribution of the structure was analyzed. It was optimized by MATLAB software. The result shows that this structure can improve access efficiency. The quality of the comb structure can be minimized under the constraints of strength requirements.

Design of an Acoustic Levitator for Three-Dimensional Manipulation of Numerous Particles

We present a design of an acoustic levitator consisting of three pairs of opposite transducer arrays.Three orthogonal standing waves create a large number of acoustic traps at which the particles are levitated in mid-air.By changing the phase difference of transducer arrays,three-dimensional manipulation of particles is successfully realized.Moreover,the relationship between the translation of particles and the phase difference is experimentally investigated,and the result is in agreement with the theoretical calculation.This design can expand the application of acoustic levitation in many fields,such as biomedicine,ultrasonic motor and new materials processing.

Three-dimensional global interconnect based on a design window

Based on a stochastic wire length distributed model, the interconnect distribution of a three-dimensional integrated circuit （3D IC） is predicted exactly. Using the results of this model, a global interconnect design window for a giga-scale system-on-chip （SOC） is established by evaluating the constraints of 1） wiring resource, 2） wiring bandwidth, and 3） wiring noise. In comparison to a two-dimensional integrated circuit （2D IC） in a 130-nm and 45-nm technology node, the design window expands for a 3D IC to improve the design reliability and system performance, further supporting 3D IC application in future integrated circuit design.

Set up the Mechanical Curriculum System Based on Three-Dimensional Design

The changes of design methods and manufacturing techniques have brought new requirements for engineers in enterprises, and therefore brought a challenge to the traditional teaching system of mechanical major courses. A new teaching system based on three-dimensional design to cultivate modern engineers with solid specialty bases and high creativity in a wide range of fields is presented.

Integration system research and development for three-dimensional laser scanning information visualization in goaf

An integration processing system of three-dimensional laser scanning information visualization in goaf was developed. It is provided with multiple functions, such as laser scanning information management for goaf, cloud data de-noising optimization, construction, display and operation of three-dimensional model, model editing, profile generation, calculation of goaf volume and roof area, Boolean calculation among models and interaction with the third party soft ware. Concerning this system with a concise interface, plentiful data input/output interfaces, it is featured with high integration, simple and convenient operations of applications. According to practice, in addition to being well-adapted, this system is favorably reliable and stable.

Optimal Design of Stewart Platform Safety Mechanism

A safety mechanism capable of moving at will within the range of its whole link lengths is designed based on the link space. Sixteen extreme poses are obtained in a Stewart platform. The singular points of the extreme poses are solved by using homotopy method as well as the judgment condition of singular points, and thereby the maximum link lengths are achieved. The rotation angles of joints and the distances between two neighboring links are analyzed in a calculation example in which that the mechanism moves among the extreme poses is assumed. Then an algorithm to test the safety mechanism is presented taking the constraint conditions into account. A safety mechanism having optimal properties of global movement is worked out by optimizing all structural parameters through minimizing the average condition number of extreme poses.

Three-dimensional(3D) Printing Technology Assisted by Minimally Invasive Surgery for Pubic Rami Fractures

The feasibility of three-dimensional (3D) printing technology cgmbined with minimally invasive surgery in the treatment of pubic rami fractures was explored.From August 2015 to October 2017,a series of 30 patients who underwent surgical stabilization of their anterior pelvic ring (all utilizing the 3D printing technology)by one surgeon at a single hospital were studied.The minimally invasive incisions were made through anterior inferior cilia spine and pubic nodule.Data collected included the operative duration,the blood loss,the damage of the important tissue,the biographic union and therecovery of the function after the operation.Measurements on inlet and outlet pelvic cardiograph were made immediately post-operation and at all follow-up clinic visits.The scores of reduction and function were measured during follow-up.Results showed that the wounds of 30 patients were healed in the first stage,and there was no injury of important structures such as blood vessels and nerves.According to the Matta criteria,excellent effectiveness was obtained in 22 cases and good in 8 cases.According to the functional evaluation criteria of Majeed,excellent effectiveness was obtained in 21 cases and good in 9 cases.It was suggested that the 3D printing technology assisted by minimally invasive surgery can better evaluate the pelvic fracture before operation,which was helpful in plate modeling, and can shorten surgery duration and reduce intraoperative blood loss and complications. The positioning accuracy was improved,and better surgical result was finally achieved.

Three-Dimensional Ocean Sensor Networks:A Survey

The past decade has seen a growing interest in ocean sensor networks because of their wide applications in marine research,oceanography,ocean monitoring,offshore exploration,and defense or homeland security.Ocean sensor networks are generally formed with various ocean sensors,autonomous underwater vehicles,surface stations,and research vessels.To make ocean sensor network applications viable,efficient communication among all devices and components is crucial.Due to the unique characteristics of underwater acoustic channels and the complex deployment environment in three dimensional(3D) ocean spaces,new efficient and reliable communication and networking protocols are needed in design of ocean sensor networks.In this paper,we aim to provide an overview of the most recent advances in network design principles for 3D ocean sensor networks,with focuses on deployment,localization,topology design,and position-based routing in 3D ocean spaces.

Concept Design,Modeling and Station-keeping Attitude Control of an Earth Observation Platform

The stratosphere airship provides a unique and promising platform for earth observation. Researches on the project design and control scheme for earth observation platforms are still rarely documented. Nonlinear dynamics, model uncertainties, and external disturbances contribute to the difficulty in maneuvering the stratosphere airship. A key technical challenge for the earth observation platform is station keeping, or the ability to remain fixed over a geo-location. This paper investigates the conceptual design, modeling and station-keeping attitude control of the near-space earth observation platform. A conceptual design of the earth observation platform is presented. The dynamics model of the platform is derived from the Newton-Euler formulation, and the station-keeping control system of the platform is formulated. The station-keeping attitude control approach for the platform is proposed. The multi-input multi-output nonlinear control system is decoupled into three single-input single-output linear subsystems via feedback linearization, the attitude controller design is carried out on the new linear systems using terminal sliding mode control, and the global stability of the closed-loop system is proven by using the Lyapunov theorem. The performance of the designed control system is simulated by using the variable step Runge-Kutta integrator. Simulation results show that the control system tracks the commanded attitude with an error of zero, which verify the effectiveness and robustness of the designed control system in the presence of parametric uncertainties. The near-space earth observation platform has several advantages over satellites, such as high resolution, fast to deploy, and convenient to retrieve, and the proposed control scheme provides an effective approach for station-keeping attitude control of the earth observation platform.

Design of A Bionic Spudcan and Analysis of Penetration and Extraction Performances for Jack-up Platform

The mechanisms of soil structure interaction have drawn much attention in the past years in the installation and operation of jack-up platform. A bionic spudcan produced by biomimetic of egg and snail shell is proposed, and the performance of the penetration and extraction are analyzed by numerical method. The geometric contour of egg and snail shell is measured, and its mathematical model is established respectively. According to the structure of existing spudcan of jack-up platform, three kinds of typical biomimetic spudcan are designed. Furthermore, numerical analysis models of biomimetic spudcan are established respectively to study the soil structure interaction mechanism in the process of penetration and extraction, and contrastive analysis of resistance characteristics are carried out. To conclude, the results show that the biomimetic spudcan facilitates the platform installation, and it is also beneficial to the improvement of the bearing capacity of spudcan.

Optimum Design of Jacket Platforms Considering Structure-Pile-Soil Interaction

This paper proposes an optimum design model for the offshore jacket platform considering multidesign criteria, multi-design constraints and the structure-pile-soil interaction, and gives an optimum design procedure in which the proposed optimum design model is used together with structural analysis software SAP91 and optimum algorithm software OPB1. The Chengbei (#)11 offshore platform, which lies in the Shengli oilfield, is designed by use of the above optimum design model. The results show that the optimum design model is stable, and it depends on neither the optimization algorithm nor initial values of design variables. All values of the objective function converge to the same minimum value, and the speed of convergence is high, showing that the proposed optimum design model is reasonable.

Parameter identification of inertially stabilized platforms using current command design

Accurate parameter identification is essential when designing controllers for inertially stabilized platforms （lSPs）. But traditional identification methods suffer from observation measurement noise and operating restrictions of ISPs. To address this issue, a novel identification method based on current command design and multilevel coordinate search （MCS） algorithm without any higher order measurement differentiations was proposed. The designed current commands were adopted to obtain parameter decoupled models with the platform operating under allowable conditions. MCS algorithm was employed to estimate the parameters based on parameter decoupled models. A comparison experiment between the proposed method and non-linear least square method was carried out and most of the relative errors of identified parameters obtained by the proposed method were below 10%. Simulation and experiment based on identified parameters were conducted. A velocity control structure was also developed with disturbance observer （DOB） for application in disturbance compensation control system of an ISR Experimental results show that the control scheme based on the identified parameters with DOB has the best disturbance rejection performance. It reduces the peak to peak value （PPV） of velocity error integral to 0.8 mrad which is much smaller than the value （10 mrad） obtained by the single velocity controller without DOB. Compared with the control scheme based on sweep model with DOB compensation, the proposed control scheme improves the PPV of velocity error integral by 1.625 times.

Optimal design and applicability of electric power steering system for automotive platform

The ongoing need for better fuel economy and lower exhaust pollution of vehicles has increased the employment of electric power steering(EPS)in automotives.Optimal design of EPS for a product family reduces the development and fabrication costs significantly.In this paper,the TOPSIS method along with the NSGA-Ⅱis employed to find an optimum family of EPS for an automotive platform.A multi-objective optimization problem is defined considering road feel,steering portability,RMS of Ackerman error,and product family penalty function(PFPF)as the conflicting objective functions.The results for the single objective optimization problems and the ones for the multi-objective optimization problem,as well as two suggested trade-off design points are presented,compared and discussed.For the two suggested points,performance at one objective function is deteriorated by about 1%,while the commonality is increased by 20%–40%,which shows the effectiveness of the proposed method in first finding the non-dominated design points and then selecting the trade-off among the obtained points.The results indicate that the obtained trade-off points have superior performance within the product family with maximum number of common parts.

Estimating Design Loads with Environmental Contour Approach Using Copulas for an Offshore Jacket Platform

Jacket-type offshore platforms are widely used for oil, gas field, and energy development in shallow water. The design of a jacket structure is highly dependent on target environmental variables. This study focuses on a strategy to estimate design loads for offshore jacket structures based on an environmental contour approach. In addition to the popular conditional distribution model, various classes of bivariate copulas are adopted to construct joint distributions of environmental variables. Analytical formulations of environmental contours based on various models are presented and discussed in this study. The design loads are examined by dynamic response analysis of jacket platform. Results suggest that the conditional model is not recommended for use in estimating design loads in sampling locations due to poor fitting results. Independent copula produces conservative design loads and the extreme response obtained using the conditional model are smaller than those determined by copulas. The suitability of a model for contour construction varies with the origin of wave data. This study provides a reference for the design load estimation of jacket structures and offers an alternative procedure to determine the design criteria for offshore structures.