Soybean hulls, an abundant byproduct of soybean processing, contain rich phytochemicals, fibers, proteins, and minerals. Currently soybean hulls are primarily used as animal feeds. For value-added soybean hull utiliza...Soybean hulls, an abundant byproduct of soybean processing, contain rich phytochemicals, fibers, proteins, and minerals. Currently soybean hulls are primarily used as animal feeds. For value-added soybean hull utilization, 25% soybean hulls were substituted for amaranth or whole oat flour (WOF) in novel gluten-free cookies. Composition, nutritional values, water-holding capacities, correlation between properties, and pasting and rheological properties of soybean hulls, amaranth, and WOF were appraised in comparison to wheat flour. Water loss, cookie texture, and geometrical properties of the cookies were examined. The results disclosed that soybean hulls, amaranth and WOF contain higher protein content, minerals, fiber, special amino acids, and critical vitamins (C and K) than wheat flour. Considerably higher total amino acid content was found in soybean hulls (18.33%) than wheat flour (12.77%). Water-holding capacities increased by replacing amaranth and WOF with soybean hulls. Soybean hulls exhibited higher rheological elastic properties than amaranth, WOF and wheat flours. The soybean hulls utilized in amaranth or WOF cookies greatly improved their nutritional value, the water retention and moisture content along with acceptable physical properties when compared to wheat flour cookies. This study explored the feasibility and potential of utilizing soybean hulls with amaranth and WOF in gluten-free bakery products and other food applications.展开更多
Background: The roll-out of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was certainly among the fastest in medical history. Vaccination campaigns around the world began a year after t...Background: The roll-out of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was certainly among the fastest in medical history. Vaccination campaigns around the world began a year after the outbreak in 2019. When Austria started vaccinating the population in 2020, we took the opportunity to collect data from the first sets of patients receiving the vaccine in our study region of East Tyrol. Purpose: Many studies have been conducted examining the immunogenicity of the new vaccines using classic serological test methods in combination with an IFN-γ ELISpot. Undeniable disadvantages of using IFN-γ to characterize the status of the cellular immunity are that 1) being an acute phase cytokine, IFN-γ loses signal strength in the long run and 2) IFN-γ does not provide information about the involvement of T helper 2 (Th2) cells in the immune process. This implies that it can affect false negative data about the cell-mediated immune status. Method: Therefore, in addition to a chemiluminescent immunoassay and the enzymatic IFN-γ ELISpot, this study included a fluorescent ELISpot assay using precoated human SARS-CoV-2-specific IFN-γ/IL-2/IL-5 ELISpot kits to show a more holistic overview on the involvement of T helper 1 (Th1) cells as signal senders of IL-2 and Th2 cells as senders of IL-5. Results and Conclusion: Our study confirms good immunogenicity of Pfizer/BioNTech BNT162b2 COVID-19 (Comirnaty) with strong Th1 and vanishingly small Th2 participation. The fluorescent three color iSpot can improve the diagnostic results’ significance for the individual, especially when the infection has been longer in the past and the IFN-γ signal diminishes.展开更多
A comparative study of microsporogenesis in male-sterile and male-fertile grain amaranth was conducted using electron-microscopy.The onset of microsporogenesis breakdown in male-sterile is at the mononuclear pollen st...A comparative study of microsporogenesis in male-sterile and male-fertile grain amaranth was conducted using electron-microscopy.The onset of microsporogenesis breakdown in male-sterile is at the mononuclear pollen stage following the release of microspores from tetrads.Abnormality in the behavior of degenerated tapetum was observed, which failed to envelope individual microspores after their release, leading to an abnormal vacuolation in the mononuclear pollen grains.As a result, the normal thickening of pollen wall could not occur and pollen grains could not engorge, causing male sterility.展开更多
The objective of the present study was to gain better insight into the physiological mechanisms on the effects of copper （Cu） on photosynthesis and active oxygen metabolism in three-colored amaranth plant （Amaranth...The objective of the present study was to gain better insight into the physiological mechanisms on the effects of copper （Cu） on photosynthesis and active oxygen metabolism in three-colored amaranth plant （Amaranthus tricolor）. Three- colored amaranth seedlings were subjected to different Cu levels in soils during the entire experimental period. The parameters of growth, photosynthesis, mineral elements contents, and active oxygen metabolism were investigated using plant physiological methods. The results showed that 2.0 and 4.0 mmol Cu kg^-1 treatments decreased the whole plant biomass to 91 and 73% of the control, respectively. The net photosynthetic rate （Pn） and the stomatal conductance （gs） were similarly reduced in the third leaves of three-colored amaranth seedlings treated with 2.0 and 4.0 mmol Cu kg^-1 soil, respectively. None of the investigated Cu levels decreased the internal CO2 concentration （Ci）. The effect of Cu on the potential efficiency of photosystem Ⅱ （Fv/Fm） was negligible, whereas the effect of Cu on the PS Ⅱ quantum efficiency （ΦPS Ⅱ） after plant adaptation in actinic irradiation was more noticeable. On the other hand, decreases in water percentage, contents of photosynthetic pigments and mineral elements including Fe, K, and Mg, and significant increase in the Cu content were observed in the third leaves of Cu-treated plants. Superoxide dismutase （SOD） and peroxidase （POD） activities as well as the proline （Pro） content significantly increased in the third leaves of the three-colored amaranth seedlings treated with 2.0 and 4.0 mmol Cu kg^-1 soil, while catalase （CAT） and ascorbate peroxidase （APX） activities as well as the contents of carotenoid （Car）, glutathione （GSH）, and ascorbic acid （AsA） decreased, and accompanied by the increases in the contents of hydrogen peroxide （H2O2）, superoxide anion （O2^-）, and malondialdehyde （MDA）, and electrolyte leakage. As a result of the imbalance of active oxygen metabolism, Pn and ΦPS Ⅱ decreased, and peroxidization enhanced under levels of 2.0 and 4.0 mmol Cu kg^-1 soil. Finally, the growth of three-colored amaranth plant was significantly inhibited.展开更多
The potential harm of soil cadmium pollution to ecological environment and human health has been increasingly widely concerned. Phytoremediation, as a kind of new and effective technology, has become an important meth...The potential harm of soil cadmium pollution to ecological environment and human health has been increasingly widely concerned. Phytoremediation, as a kind of new and effective technology, has become an important method for cleaning up cadmium in contaminated sites. The amaranth （Amaranthus mangostanus L.） is widely distributed and has abundant varieties in China, its rapid growth and large biomass can be served as candidate for cadmium hyperaccumulators for phytoremediation. To obtain cadmium hyper-accumulator, Cd uptake in 23 amaranth cultivars from different ecological region was investigated under hydroponic culture condition. Meanwhile, pot experiment was established to probe phytoremediation potentiality of Cd contaminated soil by amaranth. Three treatment （Cd 5, 10, and 25 mg kg^-1） were imposed to red soil, yellow brown soil, and vegetable soil. The results showed that under hydroponic culture with Cd 3 mg L^-1, the cadmium concentration in the shoots of the cultivar Tianxingmi reached 260 mg kg^-1, and its total cadmium uptake was the highest among various cultivars. In the treatment Cd 25 mg kg^-1, the cadmium concentration in the shoots of the cultivar Tianxingmi reached 212 mg kg^-1, while bioaccumulation factor and shoot purification rate reached 8.50 and 3.8%, respectively. Further, the total biomass and shoot biomass were not decreased significantly under Cd exposure. These results suggested that cultivar Tianxingmi is a typical Cd hyperaccumulator, and can be expected to be used in phytoremediation of Cd contaminated soil.展开更多
The growth of clones of seven Amaranthus palmeri x A. spinosus hybrids was compared to type specimens of A. palmeri and A. spinosus. The hybrids came from the field where they were originally discovered and clones of ...The growth of clones of seven Amaranthus palmeri x A. spinosus hybrids was compared to type specimens of A. palmeri and A. spinosus. The hybrids came from the field where they were originally discovered and clones of the type specimens and hybrids were established under greenhouse conditions and used to compare growth rates. A. palmeri had the highest growth rate and A. spinosus the lowest growth rate based on height, node counts, and dry weight accumulation. A. palmeri also had the greatest number of days to flowering and A. spinosus the fewest. Hybrids had intermediary growth rates and days to flowering, but differed from each other with regard to sex identity. The hybrids were either dioecious like A. palmeri or, if monoecious, had patterns unlike A. spinosus. Spine length and texture also varied in hybrids and some were without spines. Hybrid 16Ci was short compared to all others and had succulent leaves and stems, which easily separated from the plant body. These hybridizations resulted in morphologically distinct types with acquisition of physical traits intermediate to the type specimens which may drive evolution of these species.展开更多
Amaranthus cruentus is one of the sixty Amaranthus species of world. It is widely grown as a green leafy vegetable and for its grain in many tropical countries of Africa, Central and South American, Canada, Mexico and...Amaranthus cruentus is one of the sixty Amaranthus species of world. It is widely grown as a green leafy vegetable and for its grain in many tropical countries of Africa, Central and South American, Canada, Mexico and parts of Asia. Depleted soil fertility under intensive cultivation is a major limitation to production of the vegetable. The study investigated the effects of two types of poultry manure on biomass yield and nutrient uptake of Amaranth cruentus in a greenhouse at the University of Manitoba, Winnipeg, Manitoba, Canada in 2011. The experiment was a 2 × 3 × 3 factorial arranged into a Randomized Completely Block Design and replicated three times. The factors were two soil types, two sources of poultry manure and urea, and N rates at 0, 60 and 120 kg⋅N⋅ha−1. Nitrogen uptake by the plants that treated with poultry manure from Canada (PMC) and Urea was 172.5 vs. 171.4 mg⋅pot−1 and 169.5 vs. 163.7 mg⋅pot−1 respectively which were significantly greater than the poultry manure from Nigeria (PMN), 100.6 mg⋅pot−1 and 110.0 mg⋅pot−1 for Red River and Glenhope soil series, respectively. Nitrogen uptake was similar at the N rates of 60 and 120 kg⋅N⋅ha−1 (140.7 vs. 155.6 mg⋅pot−1) in the Red River soil, while N uptake at 120 kg⋅N⋅ha−1 was greater than at 60 kg⋅N⋅ha−1 in Glenhope soil. Shoot dry matter of the vegetable grown in soils treated with PMC and urea was significantly greater than from PMN in Red River soil (10.1, 9.6 and 6.7 g⋅pot−1) and in Glenhope soil (8.8, 8.1 and 6.4 g⋅pot−1). Phosphorus, K, Ca and Mg uptake at 60 and 120 kg⋅</展开更多
The kinetics of K+ absorption and the utilization of both applied K and K in a calcareous alluvisol with low available K by different genotypes of grain amaranth (Amaranthus spp.), an ancient pseudo-cereal that produc...The kinetics of K+ absorption and the utilization of both applied K and K in a calcareous alluvisol with low available K by different genotypes of grain amaranth (Amaranthus spp.), an ancient pseudo-cereal that produces a large biomass and a rich source of healthy nutrients and mineral elements, were studied by means of ion depletion technique and pot experiments. Grain amaranth had a high affinity for K+ uptake in comparison with corn and cotton. However, there were differences among Amaranthus spp. in K absorption and utilization. Some cultivars such as R104, CX-4, Du001, Hy015 and Hr029 which had a higher rate of K absorption and stronger affinity for K+ were typical varieties with high K-use efficiency (KUE). The results showed that high KUE cultivars grew quickly, possessed stronger ability to take up soil slowly available K and mineral K, and did not respond to K fertilization in the soil with low available K. Correspondingly, grain amaranth cultivars CX-77, Cr024, Vd001, Re003 and Sn003 were relatively low in KUE. Compared with high KUE cultivars, they took up more soil available K and both of their dry matter accumulation and K uptake responded to K applied significantly.展开更多
文摘Soybean hulls, an abundant byproduct of soybean processing, contain rich phytochemicals, fibers, proteins, and minerals. Currently soybean hulls are primarily used as animal feeds. For value-added soybean hull utilization, 25% soybean hulls were substituted for amaranth or whole oat flour (WOF) in novel gluten-free cookies. Composition, nutritional values, water-holding capacities, correlation between properties, and pasting and rheological properties of soybean hulls, amaranth, and WOF were appraised in comparison to wheat flour. Water loss, cookie texture, and geometrical properties of the cookies were examined. The results disclosed that soybean hulls, amaranth and WOF contain higher protein content, minerals, fiber, special amino acids, and critical vitamins (C and K) than wheat flour. Considerably higher total amino acid content was found in soybean hulls (18.33%) than wheat flour (12.77%). Water-holding capacities increased by replacing amaranth and WOF with soybean hulls. Soybean hulls exhibited higher rheological elastic properties than amaranth, WOF and wheat flours. The soybean hulls utilized in amaranth or WOF cookies greatly improved their nutritional value, the water retention and moisture content along with acceptable physical properties when compared to wheat flour cookies. This study explored the feasibility and potential of utilizing soybean hulls with amaranth and WOF in gluten-free bakery products and other food applications.
文摘Background: The roll-out of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was certainly among the fastest in medical history. Vaccination campaigns around the world began a year after the outbreak in 2019. When Austria started vaccinating the population in 2020, we took the opportunity to collect data from the first sets of patients receiving the vaccine in our study region of East Tyrol. Purpose: Many studies have been conducted examining the immunogenicity of the new vaccines using classic serological test methods in combination with an IFN-γ ELISpot. Undeniable disadvantages of using IFN-γ to characterize the status of the cellular immunity are that 1) being an acute phase cytokine, IFN-γ loses signal strength in the long run and 2) IFN-γ does not provide information about the involvement of T helper 2 (Th2) cells in the immune process. This implies that it can affect false negative data about the cell-mediated immune status. Method: Therefore, in addition to a chemiluminescent immunoassay and the enzymatic IFN-γ ELISpot, this study included a fluorescent ELISpot assay using precoated human SARS-CoV-2-specific IFN-γ/IL-2/IL-5 ELISpot kits to show a more holistic overview on the involvement of T helper 1 (Th1) cells as signal senders of IL-2 and Th2 cells as senders of IL-5. Results and Conclusion: Our study confirms good immunogenicity of Pfizer/BioNTech BNT162b2 COVID-19 (Comirnaty) with strong Th1 and vanishingly small Th2 participation. The fluorescent three color iSpot can improve the diagnostic results’ significance for the individual, especially when the infection has been longer in the past and the IFN-γ signal diminishes.
文摘A comparative study of microsporogenesis in male-sterile and male-fertile grain amaranth was conducted using electron-microscopy.The onset of microsporogenesis breakdown in male-sterile is at the mononuclear pollen stage following the release of microspores from tetrads.Abnormality in the behavior of degenerated tapetum was observed, which failed to envelope individual microspores after their release, leading to an abnormal vacuolation in the mononuclear pollen grains.As a result, the normal thickening of pollen wall could not occur and pollen grains could not engorge, causing male sterility.
基金the Natural Science Foundation of Zhejiang Province, China （Y504256）.
文摘The objective of the present study was to gain better insight into the physiological mechanisms on the effects of copper （Cu） on photosynthesis and active oxygen metabolism in three-colored amaranth plant （Amaranthus tricolor）. Three- colored amaranth seedlings were subjected to different Cu levels in soils during the entire experimental period. The parameters of growth, photosynthesis, mineral elements contents, and active oxygen metabolism were investigated using plant physiological methods. The results showed that 2.0 and 4.0 mmol Cu kg^-1 treatments decreased the whole plant biomass to 91 and 73% of the control, respectively. The net photosynthetic rate （Pn） and the stomatal conductance （gs） were similarly reduced in the third leaves of three-colored amaranth seedlings treated with 2.0 and 4.0 mmol Cu kg^-1 soil, respectively. None of the investigated Cu levels decreased the internal CO2 concentration （Ci）. The effect of Cu on the potential efficiency of photosystem Ⅱ （Fv/Fm） was negligible, whereas the effect of Cu on the PS Ⅱ quantum efficiency （ΦPS Ⅱ） after plant adaptation in actinic irradiation was more noticeable. On the other hand, decreases in water percentage, contents of photosynthetic pigments and mineral elements including Fe, K, and Mg, and significant increase in the Cu content were observed in the third leaves of Cu-treated plants. Superoxide dismutase （SOD） and peroxidase （POD） activities as well as the proline （Pro） content significantly increased in the third leaves of the three-colored amaranth seedlings treated with 2.0 and 4.0 mmol Cu kg^-1 soil, while catalase （CAT） and ascorbate peroxidase （APX） activities as well as the contents of carotenoid （Car）, glutathione （GSH）, and ascorbic acid （AsA） decreased, and accompanied by the increases in the contents of hydrogen peroxide （H2O2）, superoxide anion （O2^-）, and malondialdehyde （MDA）, and electrolyte leakage. As a result of the imbalance of active oxygen metabolism, Pn and ΦPS Ⅱ decreased, and peroxidization enhanced under levels of 2.0 and 4.0 mmol Cu kg^-1 soil. Finally, the growth of three-colored amaranth plant was significantly inhibited.
基金supported by the National Key Basic Research (973) Program of China (2002CB410809)
文摘The potential harm of soil cadmium pollution to ecological environment and human health has been increasingly widely concerned. Phytoremediation, as a kind of new and effective technology, has become an important method for cleaning up cadmium in contaminated sites. The amaranth （Amaranthus mangostanus L.） is widely distributed and has abundant varieties in China, its rapid growth and large biomass can be served as candidate for cadmium hyperaccumulators for phytoremediation. To obtain cadmium hyper-accumulator, Cd uptake in 23 amaranth cultivars from different ecological region was investigated under hydroponic culture condition. Meanwhile, pot experiment was established to probe phytoremediation potentiality of Cd contaminated soil by amaranth. Three treatment （Cd 5, 10, and 25 mg kg^-1） were imposed to red soil, yellow brown soil, and vegetable soil. The results showed that under hydroponic culture with Cd 3 mg L^-1, the cadmium concentration in the shoots of the cultivar Tianxingmi reached 260 mg kg^-1, and its total cadmium uptake was the highest among various cultivars. In the treatment Cd 25 mg kg^-1, the cadmium concentration in the shoots of the cultivar Tianxingmi reached 212 mg kg^-1, while bioaccumulation factor and shoot purification rate reached 8.50 and 3.8%, respectively. Further, the total biomass and shoot biomass were not decreased significantly under Cd exposure. These results suggested that cultivar Tianxingmi is a typical Cd hyperaccumulator, and can be expected to be used in phytoremediation of Cd contaminated soil.
文摘The growth of clones of seven Amaranthus palmeri x A. spinosus hybrids was compared to type specimens of A. palmeri and A. spinosus. The hybrids came from the field where they were originally discovered and clones of the type specimens and hybrids were established under greenhouse conditions and used to compare growth rates. A. palmeri had the highest growth rate and A. spinosus the lowest growth rate based on height, node counts, and dry weight accumulation. A. palmeri also had the greatest number of days to flowering and A. spinosus the fewest. Hybrids had intermediary growth rates and days to flowering, but differed from each other with regard to sex identity. The hybrids were either dioecious like A. palmeri or, if monoecious, had patterns unlike A. spinosus. Spine length and texture also varied in hybrids and some were without spines. Hybrid 16Ci was short compared to all others and had succulent leaves and stems, which easily separated from the plant body. These hybridizations resulted in morphologically distinct types with acquisition of physical traits intermediate to the type specimens which may drive evolution of these species.
文摘Amaranthus cruentus is one of the sixty Amaranthus species of world. It is widely grown as a green leafy vegetable and for its grain in many tropical countries of Africa, Central and South American, Canada, Mexico and parts of Asia. Depleted soil fertility under intensive cultivation is a major limitation to production of the vegetable. The study investigated the effects of two types of poultry manure on biomass yield and nutrient uptake of Amaranth cruentus in a greenhouse at the University of Manitoba, Winnipeg, Manitoba, Canada in 2011. The experiment was a 2 × 3 × 3 factorial arranged into a Randomized Completely Block Design and replicated three times. The factors were two soil types, two sources of poultry manure and urea, and N rates at 0, 60 and 120 kg⋅N⋅ha−1. Nitrogen uptake by the plants that treated with poultry manure from Canada (PMC) and Urea was 172.5 vs. 171.4 mg⋅pot−1 and 169.5 vs. 163.7 mg⋅pot−1 respectively which were significantly greater than the poultry manure from Nigeria (PMN), 100.6 mg⋅pot−1 and 110.0 mg⋅pot−1 for Red River and Glenhope soil series, respectively. Nitrogen uptake was similar at the N rates of 60 and 120 kg⋅N⋅ha−1 (140.7 vs. 155.6 mg⋅pot−1) in the Red River soil, while N uptake at 120 kg⋅N⋅ha−1 was greater than at 60 kg⋅N⋅ha−1 in Glenhope soil. Shoot dry matter of the vegetable grown in soils treated with PMC and urea was significantly greater than from PMN in Red River soil (10.1, 9.6 and 6.7 g⋅pot−1) and in Glenhope soil (8.8, 8.1 and 6.4 g⋅pot−1). Phosphorus, K, Ca and Mg uptake at 60 and 120 kg⋅</
文摘The kinetics of K+ absorption and the utilization of both applied K and K in a calcareous alluvisol with low available K by different genotypes of grain amaranth (Amaranthus spp.), an ancient pseudo-cereal that produces a large biomass and a rich source of healthy nutrients and mineral elements, were studied by means of ion depletion technique and pot experiments. Grain amaranth had a high affinity for K+ uptake in comparison with corn and cotton. However, there were differences among Amaranthus spp. in K absorption and utilization. Some cultivars such as R104, CX-4, Du001, Hy015 and Hr029 which had a higher rate of K absorption and stronger affinity for K+ were typical varieties with high K-use efficiency (KUE). The results showed that high KUE cultivars grew quickly, possessed stronger ability to take up soil slowly available K and mineral K, and did not respond to K fertilization in the soil with low available K. Correspondingly, grain amaranth cultivars CX-77, Cr024, Vd001, Re003 and Sn003 were relatively low in KUE. Compared with high KUE cultivars, they took up more soil available K and both of their dry matter accumulation and K uptake responded to K applied significantly.
