Phase Error Compensation of Three-Dimensional Reconstruction Combined with Hilbert Transform

Nonlinear response is an important factor affecting the accuracy of three-dimensional image measurement based on the fringe structured light method.A phase compensation algorithm combined with a Hilbert transform is proposed to reduce the phase error caused by the nonlinear response of a digital projector in the three-dimensional measurement system of fringe structured light.According to the analysis of the influence of Gamma distortion on the phase calculation,the algorithm establishes the relationship model between phase error and harmonic coefficient,introduces phase shift to the signal,and keeps the signal amplitude constant while filtering out the DC component.The phase error is converted to the transform domain,and compared with the numeric value in the space domain.The algorithm is combined with a spiral phase function to optimize the Hilbert transform,so as to eliminate external noise,enhance the image quality,and get an accurate phase value.Experimental results show that the proposed method can effectively improve the accuracy and speed of phase measurement.By performing phase error compensation for free-form surface objects,the phase error is reduced by about 26%,and about 27%of the image reconstruction time is saved,which further demonstrates the feasibility and effectiveness of the method.

Three-dimensional visualization of soil pore structure using computed tomography

The geometric and spatial characteristics of pore structures determine the permeability and water retention of soils, which have important effects on soil functional diversity and ecological restoration. Until recently, there have not been tools and methods to visually and quantitatively describe the characteristics of soil pores. To solve this problem, this research reconstructs the geometry and spatial distribution of soil pores by the marching cubes method, texture mapping method and the ray casting method widely used in literature. The objectives were to explore an optimal method for three-dimensional visualization of soil pore structure by comparing the robustness of the three methods on soil CT images with single pore structure and porosity ranging from low (2–5%) to high (12–18%), and to evaluate the reconstruction performance of the three methods with different geometric features. The results demonstrate that there are aliases (jagged edges) and deficiency at the boundaries of the model reconstructed by the marching cubes method and pore volumes are smaller than the ground truth, whereas the results of the texture mapping method lack the details of pore structures. For all the soil images, the ray casting method is preferable since it better preserves the pore characteristics of the ground truth. Furthermore, the ray casting method produced the best soil pore model with higher rendering speed and lower memory consumption. Therefore, the ray casting method provides a more advanced method for visualization of pore structures and provides an optional technique for the study of the transport of moisture and the exchange of air in soil.

Estimation of the Scale of Artificial Reef Sets on the Basis of Underwater 3D Reconstruction

The creation of three-dimensional models from an unorganized set of points is an active research area in computer graphics.One of the purposes of this study is to explore the 3D reconstruction of a cube-type artificial reef(CTAR)set by linear structured light and binocular stereo vision technology in an underwater environment.The experimental setup is composed of two ca-meras in a stereo vision configuration.The alpha shapes method can be used to construct a surface that most closely reflects the arti-ficial reef set described by the points.A parameter study is conducted to assess the scales of the set(i.e.,usable volume,surface area,projected area,height,and base diameter)on the basis of 3D reconstruction.Experimental results show that the quality of 3D recon-struction in an underwater environment is acceptable for estimating the scale size of the CTAR set.According to the measurement of the scale sizing of the CTAR set,the relationships between the parameters of the CTAR set and the number of CTAR modules were determined.Moreover,the usable volume of the CTAR set can be estimated depending on the basis of the number of CTAR modules.

Towards dynamic structure of biological complexes at atomic resolution by cryo-EM

Cryo-electron microscopy makes use of transmission electron microscopy to image vitrified biological samples and reconstruct their three-dimensional structures from two-dimensional projections via computational approaches. After over40 years of development, this technique is now reaching its zenith and reforming the research paradigm of modern structural biology. It has been gradually taking over X-ray crystallography as the mainstream method. In this review, we briefly introduce the history of cryo-EM, recent technical development and its potential power to reveal dynamic structures. The technical barriers and possible approaches to tackle the upcoming challenges are discussed.

Three-dimensional structure of CA1 pyramidal cells in rat hippocampus——Optical recording of LSM and computer simulation of fractal structure

The optical recording of three-dimensional(3-D)reconstruction of CA1 pyramidal cells wasderived from the studies on the CA1 region of the hippocampus in adult male Wistar rats.The recordingwas produced by the Confocal Laser Scan Microscope(LSM-10).The attemption was to outline themorphological neural network of CA1 pyramidal cells organization,following the trail of axo-dendritic connec-tions in 3-D spatial distributions among neurons.The fractal structure of neurons with their dendritic andaxonal trees using fractal algorithm was noticed,and 2—18 simulated cells were obtained using PC-486 comput-er.The simulational cells are similar in morphology to the natural CA1 hippocampal pyramidal cells.There-fore,the exploitation of an advanced neurohistological research technique combining optical recording of theLSM-10 and computer simulation of fractal structure can provide the quantitative fractal structural basis forchaosic dynamics of brain.

Cryo-electron microscopy for structural biology:current status and future perspectives

Recently, significant technical breakthroughs in both hardware equipment and software algorithms have enabled cryo-electron microscopy(cryo-EM) to become one of the most important techniques in biological structural analysis. The technical aspects of cryo-EM define its unique advantages and the direction of development. As a rapidly emerging field, cryo-EM has benefitted from highly interdisciplinary research efforts. Here we review the current status of cryo-EM in the context of structural biology and discuss the technical challenges. It may eventually merge structural and cell biology at multiple scales.

染色体三维结构的预测方法研究

目前基因组学领域中染色体三维结构重建是热点研究问题。已有相关文献表明,基因组的DNA突变、复制、胚胎的发育和转录长链非编码RNA的传播等跟染色质的三维结构有着密切的关联。Hi-C实验提供基因组位点之间的接触频率的全基因组图谱,推测反映其染色体的平均空间组织。文中通过在Hi-C数据基础上对染色体三维结构重建的相关文献进行分析,总结了目前重建染色体三维空间结构的经典算法原理和性能,以期能更深入地研究染色体三维结构重建算法,并系统的掌握三维染色体空间结构预测算法的发展方向。

基于多尺度图像重建的硅负极结构演化定量分析

为了深入了解硅基负极在循环过程中的结构演化,本文首次应用电极和颗粒水平多尺度的三维图像重建技术,对微米硅负极在电循环过程中的结构进行了可视化的定量表征;通过分析电解液中氟代碳酸乙烯酯(FEC)添加剂的影响,生动展示了硅负极的结构/成分演变与电化学性能之间的联系.利用聚焦离子束和扫描电子显微镜(FIB-SEM)技术,对电极中的三种组分(硅活性颗粒,非活性组分和孔隙)的体积和形状变化进行了定量的分析,并统计了循环过程中硅颗粒的平均尺寸.结合电化学分析结果表明,电解液FEC添加剂可以在硅负极的循环早期形成稳定的固态电解质界面(SEI),从而有效抑制硅颗粒的巨大体积膨胀和粉化现象,提高电极的循环稳定性.本文提出的方法为具有类似体积膨胀问题的电极体系提供了新的研究视角.

染色体三维结构重建方法研究

染色体三维结构重构问题是近年生物领域中基因组学的热点研究问题,是以二维交互频率数据为基础来预测其三维空间结构。最新相关实验表明染色质的三维空间结构对于基因表达、调控等方面都具有重要意义。而Hi-c数据能利用染色质交互信息形成二维接触矩阵重构出染色体三维结构。本综述以染色体三维结构重建方法为研究对象,通过对染色体三维结构重建方法进行比较分析,综述了目前基于Hi-c数据在染色体三维结构重建中的经典方法,系统介绍了染色体三维结构重建技术的发展脉络,以促进染色体三维结构重建的进一步研究。