Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model

Hypoxia is a typical feature of the tumor microenvironment,one of the most critical factors affecting cell behavior and tumor progression.However,the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells.This study reports a three-dimensional(3D)brain tumor model obtained by encapsulating U87MG(U87)cells in a hydrogel containing type I collagen.It also documents the effect of various oxygen concentrations(1%,7%,and 21%)in the culture environment on U87 cell morphology,proliferation,viability,cell cycle,apoptosis rate,and migration.Finally,it compares two-dimensional(2D)and 3D cultures.For comparison purposes,cells cultured in flat culture dishes were used as the control(2D model).Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase(G0 phase)/gap I phase(G1 phase)than those cultured in the 2D model.Besides,the two models yielded significantly different cell morphologies.Finally,hypoxia(e.g.,1%O2)affected cell morphology,slowed cell growth,reduced cell viability,and increased the apoptosis rate in the 3D model.These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function,and can be more representative of the tumor microenvironment than 2D culture systems.The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.

Virtual and augmented reality systems and three-dimensional printing of the renal model—novel trends to guide preoperative planning for renal cancer

ObjectiveThis study aimed to explore the applications of three-dimensional (3D) technology, including virtual reality, augmented reality (AR), and 3D printing system, in the field of medicine, particularly in renal interventions for cancer treatment.MethodsA specialized software transforms 2D medical images into precise 3D digital models, facilitating improved anatomical understanding and surgical planning. Patient-specific 3D printed anatomical models are utilized for preoperative planning, intraoperative guidance, and surgical education. AR technology enables the overlay of digital perceptions onto real-world surgical environments.ResultsPatient-specific 3D printed anatomical models have multiple applications, such as preoperative planning, intraoperative guidance, trainee education, and patient counseling. Virtual reality involves substituting the real world with a computer-generated 3D environment, while AR overlays digitally created perceptions onto the existing reality. The advances in 3D modeling technology have sparked considerable interest in their application to partial nephrectomy in the realm of renal cancer. 3D printing, also known as additive manufacturing, constructs 3D objects based on computer-aided design or digital 3D models. Utilizing 3D-printed preoperative renal models provides benefits for surgical planning, offering a more reliable assessment of the tumor's relationship with vital anatomical structures and enabling better preparation for procedures. AR technology allows surgeons to visualize patient-specific renal anatomical structures and their spatial relationships with surrounding organs by projecting CT/MRI images onto a live laparoscopic video. Incorporating patient-specific 3D digital models into healthcare enhances best practice, resulting in improved patient care, increased patient satisfaction, and cost saving for the healthcare system.

A sub-grid scale model for Burgers turbulence based on the artificial neural network method

The present study proposes a sub-grid scale model for the one-dimensional Burgers turbulence based on the neuralnetwork and deep learning method.The filtered data of the direct numerical simulation is used to establish thetraining data set,the validation data set,and the test data set.The artificial neural network(ANN)methodand Back Propagation method are employed to train parameters in the ANN.The developed ANN is applied toconstruct the sub-grid scale model for the large eddy simulation of the Burgers turbulence in the one-dimensionalspace.The proposed model well predicts the time correlation and the space correlation of the Burgers turbulence.

Three-dimensional structural models,evolution and petroleum geological significances of transtensional faults in the Ziyang area,central Sichuan Basin,SW China

With drilling and seismic data of Transtensional(strike-slip)Fault System in the Ziyang area of the central Sichuan Basin,SW China plane-section integrated structural interpretation,3-D fault framework model building,fault throw analyzing,and balanced profile restoration,it is pointed out that the transtensional fault system in the Ziyang 3-D seismic survey consists of the northeast-trending F_(I)19 and F_(I)20 fault zones dominated by extensional deformation,as well as 3 sets of northwest-trending en echelon normal faults experienced dextral shear deformation.Among them,the F_(I)19 and F_(I)20 fault zones cut through the Neoproterozoic to Lower Triassic Jialingjiang Formation,presenting a 3-D structure of an“S”-shaped ribbon.And before Permian and during the Early Triassic,the F_(I)19 and F_(I)20 fault zones underwent at least two periods of structural superimposition.Besides,the 3 sets of northwest-trending en echelon normal faults are composed of small normal faults arranged in pairs,with opposite dip directions and partially left-stepped arrangement.And before Permian,they had formed almost,restricting the eastward growth and propagation of the F_(I)19 fault zone.The F_(I)19 and F_(I)20 fault zones communicate multiple sets of source rocks and reservoirs from deep to shallow,and the timing of fault activity matches well with oil and gas generation peaks.If there were favorable Cambrian-Triassic sedimentary facies and reservoirs developing on the local anticlinal belts of both sides of the F_(I)19 and F_(I)20 fault zones,the major reservoirs in this area are expected to achieve breakthroughs in oil and gas exploration.

Hyper accuracy three-dimensional virtual anatomical rainbow model facilitates surgical planning and safe selective clamping during robot-assisted partial nephrectomy

ObjectiveTo highlight the role of hyper accuracy three-dimensional(3D)reconstruction in facilitating surgical planning and guiding selective clamping during robot-assisted partial nephrectomy(RAPN).MethodsA transperitoneal RAPN was performed in a 62-year-old male patient presenting with a 4 cm right anterior interpolar renal mass(R.E.N.A.L nephrometry score 7A).An abnormal vasculature was observed,with a single renal vein and two right renal arteries originating superiorly to the vein and anterior,when dividing in their segmental branches.According to the hyper accuracy 3D(HA3D^(®))rainbow model(MEDICS Srl,Turin,Italy),one branch belonging to one of the segmental arteries was feeding the tumor.This allowed for an accurate prediction of the area vascularized by each arterial branch.The 3D model was included in the intraoperative console view during the whole procedure,using the TilePro feature.A step-by-step explanation of the procedure is provided in the video attached to the present article.ResultsThe operative time was 90 min with a warm ischemia time on selective clamping of 13 min.Estimated blood loss was 180 mL.No intraoperative complication was encountered and no drain was placed at the end of the procedure.The patient was discharged on postoperative Day 2,without any early postoperative complications.The final pathology report showed a pathological tumor stage 1 clear cell renal cell carcinoma with negative surgical margins.ConclusionThe present study and the attached video illustrate the value of 3D rainbow model during the planning and execution of a RAPN with selective clamping.It shows how the surgeon can rely on this model to be more efficient by avoiding unnecessary surgical steps,and to safely adopt a“selective”clamping strategy that can translate in minimal functional impact.

Three-dimensional turbulent model of heat transfer and fluid flow in GTAW process

A two-equation K-ε turbulent fluid flow model is built to model the heat transfer and fluid flow in gas tungsten arc welding （GTAW） process of stainless steel S US310 and S US316. This model combines the buoyancy force, lorentz force and marangni force as the driving forces of thefluidflow in the weld pool. The material properties are functions of temperature in this model. The simulated results show that the molten metal flowing outward is mainly caused by the marangoni convection, which makes the weld pool become wider and shallower. The comparison of the weld pool shape of SUS310 and SUS316 shows that the slight differences of the value of thermal conductivity mainly attributes to the difference of the weld pool shape and the distinction of heat transport in laminar and turbulent model makes large diversity in the simulated results.

Leading-edge receptivity of boundary layer to three-dimensional free-stream turbulence

The laminar-turbulent transition has always been a hot topic of fluid mechanics. Receptivity is the initial stage and plays a crucial role in the entire transition process. The previous studies of receptivity focus on external disturbances such as sound waves and vortices in the free stream, whereas those on the leading-edge receptivity to the three-dimensional free-stream turbulence (FST), which is more general in the nature, are rarely reported. In consideration of this, this work is devoted to investigating the receptivity process of three-dimensional Tollmien-Schlichting (T-S) wave packets excited by the three-dimensional FST in a flat-plate boundary layer numerically. The relations between the leading-edge receptivity and the turbulence intensity are established, and the influence of the FST directions on the propagation directions and group velocities of the excited T-S wave packets is studied. Moreover, the leading-edge receptivity to the anisotropic FST is also studied. This parametric investigation can contribute to the prediction of laminar-turbulent transition.

NUMERICAL INVESTIGATION OF THREE-DIMENSIONAL VISCOUS INCOMPRESSIBLE FLOWS IN DIVERGENT CURVED CHANNELS AND TURBULENT MODEL STUDY

In order to make the numerical calculation of viscous flows more convenient for the flows in channel with complicated profile governing equations expressed in the arbitrary curvilinear coordinates were derived by means of Favre density-weighted averaged method, and a turbulent model with effect of curvature modification was also derived. The numerical calculation of laminar and turbulent flown in divergent curved channels was carried out by means of parabolizeil computation method. The calculating results were used to analyze and investigate the aerodynamic performance of talor cascades in compressors preliminarily.

Effect of RANS Turbulence Model on Aerodynamic Behavior of Trains in Crosswind

The numerical simulation based on Reynolds time-averaged equation is one of the approved methods to evaluate the aerodynamic performance of trains in crosswind.However,there are several turbulence models,trains may present different aerodynamic performances in crosswind using different turbulence models.In order to select the most suitable turbulence model,the inter-city express 2(ICE2)model is chosen as a research object,6 different turbulence models are used to simulate the flow characteristics,surface pressure and aerodynamic forces of the train in crosswind,respectively.6 turbulence models are the standard k-ε,Renormalization Group(RNG)k-ε,Realizable k-ε,Shear Stress Transport(SST)k-ω,standard k-ωand Spalart-Allmaras(SPA),respectively.The numerical results and the wind tunnel experimental data are compared.The results show that the most accurate model for predicting the surface pressure of the train is SST k-ω,followed by Realizable k-ε.Compared with the experimental result,the error of the side force coefficient obtained by SST k-ωand Realizable k-εturbulence model is less than 1%.The most accurate prediction for the lift force coefficient is achieved by SST k-ω,followed by RNG k-ε.By comparing 6 different turbulence models,the SST k-ωmodel is most suitable for the numerical simulation of the aerodynamic behavior of trains in crosswind.

New perspective in statistical modeling of wall-bounded turbulence

Despite dedicated effort for many decades,statistical description of highly technologically important wall turbulence remains a great challenge.Current models are unfortunately incomplete,or empirical,or qualitative.After a review of the existing theories of wall turbulence,we present a new framework,called the structure ensemble dynamics （SED）,which aims at integrating the turbulence dynamics into a quantitative description of the mean flow.The SED theory naturally evolves from a statistical physics understanding of non-equilibrium open systems,such as fluid turbulence, for which mean quantities are intimately coupled with the fluctuation dynamics.Starting from the ensemble-averaged Navier-Stokes（EANS） equations,the theory postulates the existence of a finite number of statistical states yielding a multi-layer picture for wall turbulence.Then,it uses order functions（ratios of terms in the mean momentum as well as energy equations） to characterize the states and transitions between states.Application of the SED analysis to an incompressible channel flow and a compressible turbulent boundary layer shows that the order functions successfully reveal the multi-layer structure for wall-bounded turbulence, which arises as a quantitative extension of the traditional view in terms of sub-layer,buffer layer,log layer and wake. Furthermore,an idea of using a set of hyperbolic functions for modeling transitions between layers is proposed for a quantitative model of order functions across the entire flow domain.We conclude that the SED provides a theoretical framework for expressing the yet-unknown effects of fluctuation structures on the mean quantities,and offers new methods to analyze experimental and simulation data.Combined with asymptotic analysis,it also offers a way to evaluate convergence of simulations.The SED approach successfully describes the dynamics at both momentum and energy levels, in contrast with all prevalent approaches describing the mean velocity profile only.Moreover,the SED theoretical framework is general,independent of the flow system to study, while the actual functional form of the order functions may vary from flow to flow.We assert that as the knowledge of order functions is accumulated and as more flows are analyzed, new principles（such as hierarchy,symmetry,group invariance,etc.） governing the role of turbulent structures in the mean flow properties will be clarified and a viable theory of turbulence might emerge.

Effect of Time Step Size and Turbulence Model on the Open Water Hydrodynamic Performance Prediction of Contra-Rotating Propellers

A growing interest has been devoted to the contra-rotating propellers （CRPs） due to their high propulsive efficiency, torque balance, low fuel consumption, low cavitations, low noise performance and low hull vibration. Compared with the single-screw system, it is more difficult for the open water performance prediction because forward and aft propellers interact with each other and generate a more complicated flow field around the CRPs system. The current work focuses on the open water performance prediction of contra-rotating propellers by RANS and sliding mesh method considering the effect of computational time step size and turbulence model. The validation study has been performed on two sets of contra-rotating propellers developed by David W Taylor Naval Ship R ＆ D center. Compared with the experimental data, it shows that RANS with sliding mesh method and SST k-ω turbulence model has a good precision in the open water performance prediction of contra-rotating propellers, and small time step size can improve the level of accuracy for CRPs with the same blade number of forward and aft propellers, while a relatively large time step size is a better choice for CRPs with different blade numbers.

A USM-Θ two-phase turbulence model for simulating dense gas-particle flows

A second-order moment two-phase turbulence model for simulating dense gas-particle flows （USM-Θ model）, combining the unified second-order moment twophase turbulence model for dilute gas-particle flows with the kinetic theory of particle collision, is proposed. The interaction between gas and particle turbulence is simulated using the transport equation of two-phase velocity correlation with a two-time-scale dissipation closure. The proposed model is applied to simulate dense gas-particle flows in a horizontal channel and a downer. Simulation results and their comparison with experimental results show that the model accounting for both anisotropic particle turbulence and particle-particle collision is obviously better than models accounting for only particle turbulence or only particle-particle collision. The USM-Θ model is also better than the k-ε-kp-Θ model and the k-ε-kp-εp-Θ model in that the first model can simulate the redistribution of anisotropic particle Reynolds stress components due to inter-particle collision, whereas the second and third models cannot.

DEVELOPMENT OF A HYBRID MODEL FOR THREE-DIMENSIONAL GIS

This paper presents a hybrid model for three-dimensional Geographical Information Systems which is an integration of surface- and volume-based models. The Triangulated Irregular Network (TIN) and octree models are integrated in this hybrid models. The TIN model works as a surface-based model which mainly serves for surface presentation and visualization. On the other hand, the octree encoding supports volumetric analysis. The designed data structure brings a major advantage in the three-dimensional selective retrieval. This technique increases the efficiency of three-dimensional data operation.

Modeling of Fluid Turbulence Modification Using Two-time-scale Dissipation Models and Accounting for the Particle Wake Effect

Presently developed two-phase turbulence models under-predict the gas turbulent fluctuation, because their turbulence modification models cannot fully reflect the effect of particles. In this paper, a two-time-scale dis- sipation model of turbulence modification, developed for the two-phase velocity correlation and for the dissipation rate of gas turbulent kinetic energy, is proposed and used to simulate sudden-expansion and swirling gas-particle flows. The proposed two-time scale model gives better results than the single-time scale model. Besides, a gas tur- bulence augmentation model accounting for the finite-size particle wake effect in the gas Reynolds stress equation is proposed. The proposed turbulence modification models are used to simulate two-phase pipe flows. It can prop- erly predict both turbulence reduction and turbulence enhancement for a certain size of particles observed in ex- periments.

A three-dimensional semi-implicit unstructured grid finite volume ocean model

A new three-dimensional semi-implicit finite-volume ocean model has been developed for simulating the coastal ocean circulation, which is based on the staggered C-unstructured non-orthogonal grid in the hor- izontal direction and z-level grid in the vertical direction. The three-dimensional model is discretized by the semi-implicit finite-volume method, in that the free-surface and the vertical diffusion are semi-implicit, thereby removing stability limitations associated with the surface gravity wave and vertical diffusion terms. The remaining terms in the momentum equations are discretized explicitly by an integral method. The partial cell method is used for resolving topography, which enables the model to better represent irregular topography. The model has been tested against analytical cases for wind and tidal oscillation circulation, and is applied to simulating the tidal flow in the Bohal Sea. The results are in good agreement both with the analytical solutions and measurement results.

Applying resolved-scale linearly forced isotropic turbulence in rational subgrid-scale modeling

In previous attempts of rational subgrid-scale (SGS) modeling by employing the Kolmogorov equation of filtered (KEF) quantities, it was necessary to assume that the resolved-scale second-order structure function is stationary. Forced isotropic turbulence is often used as a framework for establishing and validating such SGS models based on stationary restrictions, for it generates statistical stationary samples. However, traditional forcing method at low wavenumbers cannot provide an analytic form of forcing term for a complete KEF in physical space, which has been illustrated to be essential in the modeling of such SGS models. Thus, an alternative forcing method giving an analytic forcing term in physical space is needed for rational SGS modeling. Giving an analytic linear driving term in physical space, linearly forced isotropic turbulence should be considered an ideal theoretical framework for rational SGS modeling. In this paper, we demonstrate the feasibility of establishing a rational SGS model with stationary restriction based on linearly forced isotropic turbulence. The performance of this rational SGS model is validated. We, therefore, propose the use of linearly forced isotropic turbulence as a complement to free-decaying isotropic turbulence and low-wavenumber forced isotropic turbulence for SGS model validations.

Mechanism of local scour around submarine pipelines based on numerical simulation of turbulence model

The mechanism of local scour around submarine pipelines is studied numerically based on a renormalized group （RNG） turbulence model. To validate the numerical model, the equilibrium profiles of local scour for two cases are simulated and compared with the experimental data. It shows that the RNG turbulence model can give an appropriate prediction for the configuration of equilibrium scour hole, and it is applicable to this situation. The local scour mechanism around submarine pipelines including the flow structure, shear stress distribution and pressure field is then analyzed and compared with experiments. For further comparison and validation, especially for the flow structure, a numerical calculation employing the large eddy simulation （LES） is also conducted. The numerical results of RNG demonstrate that the critical factor governing the equilibrium profile is the seabed shear stress distribution in the case of bed load sediment transport, and the two-equation RNG turbulence model coupled with the law of wall is capable of giving a satisfying estimation for the bed shear stress. Moreover, the piping phenomena due to the great difference of pressure between the upstream and downstream parts of pipelines and the vortex structure around submarine pipelines are also simulated successfully, which are believed to be the important factor that lead to the onset of local scour.

Three-dimensional numerical modeling of gravity anomalies based on Poisson equation in spacewavenumber mixed domain

In gravity-anomaly-based prospecting, the computational and memory requirements for practical numerical modeling are potentially enormous. Achieving an efficient and precise inversion for gravity anomaly imaging over large-scale and complex terrain requires additional methods. To this end, we have proposed a new topography-capable By performing a two-dimensional Fourier transform in the horizontal directions, threedimensional partial differential equations in the spatial domain were transformed into a group of independent, one-dimensional differential equations engaged with different wave numbers. These independent differential equations are highly parallel across different wave numbers. differential equations with different wave numbers, and the efficiency of solving fixedbandwidth linear equations was further improved by a chasing method. In a synthetic test, a prism model was used to verify the accuracy and reliability of the proposed algorithm by comparing the numerical solution with the analytical solution. We studied the computational precision and efficiency with and without topography using different Fourier transform methods. The results showed that the Guass-FFT method has higher numerical precision, while the standard FFT method is superior, in terms of computation time, for inversion and quantitative interpretation under complicated terrain.

Numerical prediction of inner turbulent flow in conical diffuser by using a new five-point scheme and DLR k-ε turbulence model

The internal turbulent flow in conical diffuser is a very complicated adverse pressure gradient flow.DLR k-ε turbulence model was adopted to study it.The every terms of the Laplace operator in DLR k-ε turbulence model and pressure Poisson equation were discretized by upwind difference scheme.A new full implicit difference scheme of 5-point was constructed by using finite volume method and finite difference method.A large sparse matrix with five diagonals was formed and was stored by three arrays of one dimension in a compressed mode.General iterative methods do not work wel1 with large sparse matrix.With algebraic multigrid method(AMG),linear algebraic system of equations was solved and the precision was set at 10-6.The computation results were compared with the experimental results.The results show that the computation results have a good agreement with the experiment data.The precision of computational results and numerical simulation efficiency are greatly improved.

Turbulence and cavitation models for time-dependent turbulent cavitating flows

Cavitation typically occurs when the fluid pressure is lower than the vapor pressure at a local thermodynamic state, and the flow is frequently unsteady and turbulent. To assess the state-of-the-art of computational capabilities for unsteady cavitating flows, different cavitation and turbulence model combinations are conducted. The selected cavitation models include several widely-used models including one based on phenomenological argument and the other utilizing interface dynamics. The k-e turbulence model with additional implementation of the filter function and density correction function are considered to reduce the eddy viscosity according to the computed turbulence length scale and local fluid density respectively. We have also blended these alternative cavitation and lustrate that the eddy viscosity turbulence treatments, to ilnear the closure region can significantly influence the capture of detached cavity. From the experimental validations regarding the force analysis, frequency, and the cavity visualization, no single model combination performs best in all aspects. Furthermore, the implications of parameters contained in different cavitation models are investigated. The phase change process is more pronounced around the detached cavity, which is better illustrated by the interfacial dynamics model. Our study provides insight to aid further modeling development.