Objective:To explore whether thrombopoietin can exert a protective effect against doxorubicin-induced cardiotoxicity by modulating the sirtuin 1(SIRT1)signaling pathway.Methods:H9c2 cell viability was determined by CC...Objective:To explore whether thrombopoietin can exert a protective effect against doxorubicin-induced cardiotoxicity by modulating the sirtuin 1(SIRT1)signaling pathway.Methods:H9c2 cell viability was determined by CCK-8 and cardiomyocyte apoptosis was detected by TUNEL assay.The protein expressions of SIRT1 and p38 MAPK were measured by Western blot.RT-qPCR was also used to determine SIRT1 mRNA expression.In addition,intracellular reactive oxygen species levels and antioxidant enzyme activities were evaluated.Results:Thrombopoietin treatment reversed doxorubicin-induced decline in H9c2 cell viability.It also increased SIRT1 and decreased p-p38 MAPK protein expressions.In addition,thrombopoietin significantly attenuated doxorubicin-induced apoptosis and oxidative stress,and enhanced antioxidant enzyme activities.However,silencing SIRT1 abrogated the protective effects of thrombopoietin,as evidenced by reduced cell viability and increased oxidative stress and reactive oxygen species levels.Conclusions:Thrombopoietin alleviates doxorubicin-induced cardiomyocyte injury by reducing oxidative stress and apoptosis via the SIRT1/p38 MAPK pathway.However,its protective effects need to be further verified in animal tests.展开更多
当代女性着装裸露度呈现多样化、包容化的高裸露度,但是着装裸露度在不同场合下有着明显的差异,在工作场合裸露度相比非工作场合较低。在男性视角下,对于女性着装裸露度在不同时间、不同地点、不同场合等维度有不同的偏好标准。本文基...当代女性着装裸露度呈现多样化、包容化的高裸露度,但是着装裸露度在不同场合下有着明显的差异,在工作场合裸露度相比非工作场合较低。在男性视角下,对于女性着装裸露度在不同时间、不同地点、不同场合等维度有不同的偏好标准。本文基于医学上界定皮肤烧伤面积的“九法则”原理结合TPO理论,通过Eyetech Digital Systems软件对男性进行视觉测试,得出男性观看女性身体的视线规律,分析不同女性着装裸露度对于男性测试者的接受程度。展开更多
基金supported by the Natural Science Foundation of Hainan Province High-level Talent Project(grant number 820RC644)Innovative Research Projects for Postgraduate Students at Hainan Medical University(grant number HYYS2022B08).
文摘Objective:To explore whether thrombopoietin can exert a protective effect against doxorubicin-induced cardiotoxicity by modulating the sirtuin 1(SIRT1)signaling pathway.Methods:H9c2 cell viability was determined by CCK-8 and cardiomyocyte apoptosis was detected by TUNEL assay.The protein expressions of SIRT1 and p38 MAPK were measured by Western blot.RT-qPCR was also used to determine SIRT1 mRNA expression.In addition,intracellular reactive oxygen species levels and antioxidant enzyme activities were evaluated.Results:Thrombopoietin treatment reversed doxorubicin-induced decline in H9c2 cell viability.It also increased SIRT1 and decreased p-p38 MAPK protein expressions.In addition,thrombopoietin significantly attenuated doxorubicin-induced apoptosis and oxidative stress,and enhanced antioxidant enzyme activities.However,silencing SIRT1 abrogated the protective effects of thrombopoietin,as evidenced by reduced cell viability and increased oxidative stress and reactive oxygen species levels.Conclusions:Thrombopoietin alleviates doxorubicin-induced cardiomyocyte injury by reducing oxidative stress and apoptosis via the SIRT1/p38 MAPK pathway.However,its protective effects need to be further verified in animal tests.
文摘当代女性着装裸露度呈现多样化、包容化的高裸露度,但是着装裸露度在不同场合下有着明显的差异,在工作场合裸露度相比非工作场合较低。在男性视角下,对于女性着装裸露度在不同时间、不同地点、不同场合等维度有不同的偏好标准。本文基于医学上界定皮肤烧伤面积的“九法则”原理结合TPO理论,通过Eyetech Digital Systems软件对男性进行视觉测试,得出男性观看女性身体的视线规律,分析不同女性着装裸露度对于男性测试者的接受程度。