RNPS1 stabilizes NAT10 protein to facilitate translation in cancer via tRNA ac^(4)C modification

Existing studies have underscored the pivotal role of N-acetyltransferase 10(NAT10) in various cancers. However, the outcomes of protein-protein interactions between NAT10 and its protein partners in head and neck squamous cell carcinoma(HNSCC) remain unexplored. In this study, we identified a significant upregulation of RNA-binding protein with serine-rich domain 1(RNPS1) in HNSCC, where RNPS1 inhibits the ubiquitination degradation of NAT10 by E3 ubiquitin ligase, zinc finger SWIM domain-containing protein 6(ZSWIM6), through direct protein interaction, thereby promoting high NAT10 expression in HNSCC. This upregulated NAT10 stability mediates the enhancement of specific tRNA ac^(4)C modifications, subsequently boosting the translation process of genes involved in pathways such as IL-6 signaling, IL-8 signaling, and PTEN signaling that play roles in regulating HNSCC malignant progression, ultimately influencing the survival and prognosis of HNSCC patients. Additionally, we pioneered the development of TRMC-seq, leading to the discovery of novel t RNA-ac^(4)C modification sites, thereby providing a potent sequencing tool for tRNAac^(4)C research. Our findings expand the repertoire of tRNA ac^(4)C modifications and identify a role of tRNA ac^(4)C in the regulation of mRNA translation in HNSCC.

Petrogenesis and Tectonic Implications of the Early Triassic Nianzi Adakitic Granite Unit in the Yanshan Fold and Thrust Belt:New Constraints from U-Pb Geochronology and Sr-Nd-Hf Isotopes

The Nianzi granite unit,which includes the Nianzi,Xiaolianghou and Xiawopu granitic intrusions,is a significant component of the northern part of the North China Craton(NCC)and is situated in the Yanshan fold and thrust belt(YFTB).However,there is still debate regarding the tectonic evolutionary history of the YFTB during the late Permian to Triassic period,specifically regarding the timing of subduction and collision between the NCC and the Paleo-Asian Ocean.The Nianzi granite unit exhibits unique petrological,geochronological and geochemical signatures that shed light on the tectonic evolutionary history of the YFTB.This study presents detailed petrology,whole-rock geochemistry,together with Sr-Nd isotopic,zircon U-Pb dating and Lu-Hf isotopic data of the granites within the Nianzi granite unit.Our findings demonstrate that the granites primarily consist of subhedral K-feldspar,plagioclase,quartz,minor biotite and hornblende,with accessory titanite,apatite,magnetite and zircon.Zircon U-Pb dating indicates that the Xiaolianghou granite was emplaced at 247.5±0.62 Ma.Additionally,the adakitic characteristics of the Nianzi,Xiawopu and Xiaolianghou granitic intrusions,such as high Sr and Ba contents and high ratios of Sr/Y and(La/Yb)N,combined with negative Sr-Nd and Lu-Hf isotopes(87Sr/86Sr)i=0.705681–0.7057433,εNd(t)=−21.98 to−20.97,zirconεHf(t)=−20.26 to−9.92,as well as the I-type granite features of high SiO_(2),Na_(2)O and K_(2)O/Na_(2)O ratios,enriched Rb,K,Sr and Ba,along with depleted Th,U,Nb,Ta,P and Ti,suggest that the Nianzi granitic unit was mainly derived from the partial melting of a thickened lower crust containing hydrous,calc-alkaline to high-K calc-alkaline,mafic to intermediate metamorphic rocks.In light of these parameters,we further integrate our data with previous studies and conclude that the Nianzi granitic unit was generated in a post-collisional extensional environment during the Early Triassic.

IPSec的NAT穿越技术应用

在总部和分部之间建立IPSec隧道,当分部与总部的专线出现故障时,这是一种安全有效的连接方案。但在实际连接过程中,有的分部只能先由网络中的NAT设备进行地址转换才能访问Internet,而IPSec不允许修改数据包。通过分析IPSec协议2种工作模式下数据包封装格式,给出了IPSec的NAT穿越步骤以及具体实例,对IPsec的NAT穿越技术应用具有一定的借鉴意义。

Thrust Optimization of Flapping Wing via Gradient Descent Technologies

The current work aims at employing a gradient descent algorithm for optimizing the thrust of a flapping wing. An in-house solver has been employed, along with mesh movement methodologies to capture the dynamics of flow around the airfoil. An efficient framework for implementing the coupled solver and optimization in a multicore environment has been implemented for the generation of optimized solutionsmaximizing thrust performance & computational speed.

Twice NAT在防火墙部署中的探讨与应用

Twice NAT,顾名思义,两次NAT,也称为策略NAT。原本的NAT技术是为了解决IPv4公网地址枯竭的问题而诞生的技术,NAT技术除了能够转换IP地址之外,也能够对给定协议的端口号进行转换,且NAT技术具有隐藏内部IP地址的作用,有一定的安全性。Twice NAT提供了另外一种内、外网设备地址互相转换的灵活性,解决了一些场景下的设备互访问题。本研究主要以防火墙的Twice NAT转换逻辑、配置验证为基础,探讨Twice NAT转换的思路,望能为计算机网络设备配置以及安全设备配置提供一些有参考价值的内容。

基于NATCA-GreaterYOLO的航拍小目标检测

为解决通用目标检测模型在无人机航拍场景下存在的物体尺度变化剧烈及复杂的背景干扰等问题,本文主要对基于NATCA-Greater YOLO的航拍小目标检测进行研究。在特征提取网络的最后一层,加入邻域注意力转换器(neighborhood attention transformer,NAT),以保留足够的全局上下文信息,并提取更多不同的特征。同时,在特征融合网络(Neck)部分,加入坐标注意力(coordinate attention,CA)模块,以获取通道信息和更长范围的位置信息,将原卷积块中的激活函数替换为Meta-ACON,并使用NAT作为新网络的预测层,以VisDrone2019-DET目标检测数据集为基准,在VisDrone2019-DET-test-dev数据集上进行测试。为了评估NATCA-Greater YOLO模型在航拍小目标检测任务中的有效性,采用Faster R-CNN、RetinaNet和单步多框目标检测(single shot multiBox detector,SSD)等检测网络在测试集上进行对比检测。研究结果表明,NATCA-Greater YOLO检测的平均精度为42%,与最先进的检测网络TPH-YOLOv5相比,NATCA-Greater YOLO的检测精度提升了2.9%,说明该模型可以准确地定位并识别目标。该研究具有一定的创新性。

Large-scale thrusting at the northern Junggar Basin since Cretaceous and its implications for the rejuvenation of the Central Asian Orogenic Belt

The Wulungu Depression is the northernmost first-order tectonic unit in the Junggar Basin. It can be divided into three sub-units： the Hongyan step-fault zone, the Suosuoquan sag and the Wulungu south slope. The Cenozoic strata in the basin are intact and Mesozoic-Cenozoic deformation can be observed in the Wulungu step-fault zone, so this is an ideal place to study the Mesozoic-Cenozoic deformation. By integration of fault-related folding theories, regional geology and drilling data, the strata of the Cretaceous-Paleogene systems are divided into small layers which are selected as the subjects of this research. The combination of the developing unconformity with existing growth strata makes it conceivable that faults on the step-fault zone have experienced different degrees of reactivation of movement since the Cretaceous. Evolutionary analyses of the small layers using 2D-Move software showed certain differences in the reactivation of different segments of the Wulungu Depression such as the timing of reactivation of thrusting, for which the reactivity time of the eastern segment was late compared with those of the western and middle segments. In addition the resurrection strength was similarly slightly different, with the shortening rate being higher in the western segment than in the other segments. Moreover, the thrust fault mechanism is basement-involved combined with triangle shear fold, for which a forward evolution model was proposed.

Critical Thrust Force Forecasting for Drilling Exit Delamination of Carbon Fibre Reinforced Plastics

Exit delamination is excessive drilling thrust force.Therefore,it is necessary to investigate the critical thrust force which cause exit delamination when carbon fibre reinforced plastics(CRFP)is drilled.According to the linear elastic fracture mechanics,the mechanics of composite material and the classical thin plate bending theory,a common theoretical model of the critical drilling thrust force for CFRP plates is established.Compared with the experimental data of previous studies,the results show that the theoretical values agree well with the experimental values.This model can be used to forecast the critical thrust force for the drilling-induced delamination of CFRP.

HBsAg不同状态下NAT反应性献血者血清学标志物特征分析

目的 了解HBsAg不同状态下核酸检测(NAT)反应性献血者HBV血清学标志物特征。方法 收集2021年9月~2022年5月核酸检测反应性标本,且ELISA检测HBsAg-/初筛HBsAg+复检阴性/单试剂HBsAg+标本,TMA鉴别非反应性标本加做罗氏PCR单人份复检,用电化学发光免疫法(ECLI)定量检测乙肝两对半进行统计分析。结果 共收集66份标本,HBsAg-/NAT+标本55份,抗-HBc阳性率为87.3%(48/55),抗-HBs、抗-HBc 2项阳性率为43.6%(24/55),抗-HBe、抗-HBc 2项阳性率为45.5%(25/55),单项抗-HBs阳性率为10.9%(6/55)和全阴性占比1.8%(1/55);酶免初筛HBsAg+复检阴性/NAT+标本7份,抗-HBc阳性率为100%(7/7),抗-HBe、抗-HBc 2项阳性率为71.4%(5/7);单试剂HBsAg+/NAT+标本4份,HBsAg、抗-HBs、抗-HBe、抗-HBc 4项阳性率为50%(2/4),抗-HBe、抗-HBc 2项阳性率为100%(4/4)。TMA鉴别非反应性且抗-HBc-标本PCR单人份复检为非反应性。在HBsAg-/NAT+标本与单试剂HBsAg+/NAT+标本中抗-HBe、抗-HBc 2项均阳性有差异(P<0.05)。结论 HBsAg-/NAT+献血者大多数为隐匿性乙型肝炎病毒感染,抗-HBe、抗-HBc 2项均阳性与HBV感染状态具有相关性,TMA鉴别非反应性且抗-HBc-不排除HBV DNA非反应性。

Early Cretaceous Thrust and Nappe Tectonics in North Qilian Shan,Northern Tibetan Plateau:Evidence from Field Mapping,Geochronology,and Deep Structural Analysis

The North Qilian Shan fold and thrust belt,located at the northern Tibetan Plateau and southern margin of the Hexi Corridor,is a key tectonic unit to decode the formation and expansion of the plateau.Previous studies emphasize the Cenozoic deformation due to the far-field response to the Indo-Asian collision,but the Mesozoic deformations are poorly constrained in this area.We conducted detailed field mapping,structural analysis,geochronology,and structural interpretation of deep seismic reflectional profiling and magnetotelluric(MT)sounding,to address the superposed results of the Mesozoic and Cenozoic deformation.The results recognized the North Qilian thrust and nappe system(NQTS),the root and the frontal belt are the North Qilian thrust(NQT),and the Yumu Shan klippe(YK),respectively.The middle belt is located between the NQT and the YK.Monzonitic granite zircon U-Pb dating from the middle belt yields an age of ca.415 Ma,which is similar to south NQT.The thrusting displacement is estimated at ca.48 km by structural interpretation of deep profiles.The timing is constrained in the early stage of the Early Cretaceous by the formation of simultaneous growth strata.We suggest that the NQTS has resulted from the far-field effect of the Lhasa-Qiangtang collision,and the Yumu Shan is uplifted by the superposed Cenozoic deformation.

Heterogeneity and differential hydrocarbon accumulation model of deep reservoirs in foreland thrust belts: A case study of deep Cretaceous Qingshuihe Formation clastic reservoirs in southern Junggar Basin, NW China

Using the data of drilling, logging, core, experiments and production, the heterogeneity and differential hydrocarbon accumulation model of deep reservoirs in Cretaceous Qingshuihe Formation(K1q) in the western section of the foreland thrust belt in southern Junggar Basin are investigated. The target reservoirs are characterized by superimposition of conglomerates, sandy conglomerates and sandstones, with high content of plastic clasts. The reservoir space is mainly composed of intergranular pores. The reservoirs are overall tight, and the sandy conglomerate has the best physical properties. The coupling of short deep burial period with low paleotemperature gradient and formation overpressure led to the relatively weak diagenetic strength of the reservoirs. Specifically, the sandy conglomerates show relatively low carbonate cementation, low compaction rate and high dissolution porosity. The special stress-strain mechanism of the anticline makes the reservoirs at the top of the anticline turning point more reformed by fractures than those at the limbs, and the formation overpressure makes the fractures in open state. Moreover, the sandy conglomerates have the highest oil saturation. Typical anticline reservoirs are developed in deep part of the thrust belt, but characterized by "big trap with small reservoir". Significantly, the sandy conglomerates at the top of anticline turning point have better quality, lower in-situ stress and higher structural position than those at the limbs,with the internal hydrocarbons most enriched, making them high-yield oil/gas layers. The exponential decline of fractures makes hydrocarbon accumulation difficult in the reservoirs at the limbs. Nonetheless, plane hydrocarbon distribution is more extensive at the gentle limb than the steep limb.

献血者HBsAg阴性NAT可疑标本HBV感染状态确认

目的了解天津地区无偿献血者应用PANTHER单人份核酸检测(ID-NAT)后,联检反应性,鉴别试验阴性(NAT可疑)标本HBV感染情况。方法本中心2021年1—8月69362份无偿献血者标本经常规检测和ID-NAT检测后,共检出HBsAg-PANTHER核酸检测联检反应性而鉴别非反应性献血者标本(以下简称NAT可疑标本)66份。采用单纯随机抽样方法选取23份应用超高速离心富集病毒后,采用超敏荧光定量PCR(qPCR)检测HBV DNA,ID-NAT复测鉴别实验,补充电化学发光法乙肝两对半检测。结果23份NAT可疑标本中经血清学和分子生物学检测共确认HBV DNA阳性14份,HBV感染者抗-HBc阳性率为92.8%。感染者中92.8%(13/14)为隐匿性乙型肝炎病毒感染(OBI)。超敏荧光定量PCR检测共有10份标本获得病毒载量,5份可定量,病毒载量定量(11~464)IU/mL,中位数为15.4 IU/mL。结论NAT可疑标本中仍能检出60%标本为HBV DNA阳性。抗-HBc检测能排除大多数NAT无法检测到的OBI,应提高目前NAT检测灵敏度,确保输血安全。

Study of beam divergence and thrust vector eccentricity characteristics of the Hall thruster based on dual Faraday probe array planes and its applications

The accurate knowledge of the thrust vector eccentricity and beam divergence characteristics of Hall thrusters are of significant engineering value for the beneficial integration and successful application of Hall thrusters on spacecraft.For the characteristics of the plume bipolar diffusion due to the annular discharge channel of the Hall thruster,a Gaussian-fitted method for thrust vector deviation angle and beam divergence of Hall thrusters based on dual Faraday probe array planes was proposed in respect of the Hall thruster beam characteristics.The results show that the ratios of the deviation between the maximum and minimum values of the beam divergence angle and the thrust vector eccentricity angle using a Gaussian fit to the optimized Faraday probe dual plane to the mean value are 1.4%and 11.5%,respectively.The optimized thrust vector eccentricity angle obtained has been substantially improved,by approximately 20%.The beam divergence angle calculated using a Gaussian fitting to the optimized Faraday probe dual plane is approximately identical to the non-optimized one.The beam divergence and thrust vector eccentricity angles for different anode mass flow rates were obtained by averaging the beam divergence and thrust vector eccentricity angles calculated by the dual-plane,Gaussian-fitted ion current density method for different cross-sections.The study not only allows for an immediate and effective tool for determining the design of thrust vector adjustment mechanisms of spacecraft with different power Hall thrusters but also for characterizing the 3D spatial distribution of the Hall thruster plume.

Method of SLAS’s ground track manipulation based on tangential impulse thrust

Satellites with altitudes below 400 km are called super low altitude satellites(SLAS),often used to achieve responsive imaging tasks.Therefore,it is important for the manipulation of its ground track.Aiming at the problem of ground track manipulation of SLAS,a control method based on tangential impulse thrust is proposed.First,the equation of the longitude difference between SLAS and the target point on the target latitude is derived based on Gauss’s variational equations.On this basis,the influence of the tangential impulse thrust on the ground track’s longitude is derived.Finally,the method for ground track manipulation of SLAS under the tangential impulse thrust is proposed.The simulation results verify the effective-ness of the method,after manipulation,the satellite can visit the target point and revisit it for multiple days.

An alternative interpretation for the map expression of "abrupt" changes in lateral stratigraphic level near transverse zones in fold-thrust belts

The map expression of ＂abrupt＂ changes in lateral stratigraphic level of a thrust fault has been traditionally interpreted to be a result of the presence of （1） a lateral （or oblique） thrust-ramp, or （2） a frontal ramp with displacement gradient, and/or （3） a combination of these geometries. These geometries have been used to interpret the structures near transverse zones in fold-thrust belts （FTB）. This contribution outlines an alternative explanation that can result in the same map pattern by lateral variations in stratigraphy along the strike of a low angle thrust fault. We describe the natural example of the Leamington transverse zone, which marks the southern margin of the Pennsylvanian-Permian Oquirrh basin with genetically related lateral stratigraphic variations in the North American Sevier FTB. Thus, the observed map pattern at this zone is closely related to lateral stratigraphic variations along the strike of a horizontal fault. Even though the present-day erosional level shows the map pattern that could be interpreted as a lateral ramp, the observed structures along the Leamington zone most likely share the effects of the presence of a lateral （or oblique） ramp, lateral stratigraphic variations along the fault trace, and the displacement gradient.

Winding Function Theory Based Thrust Calculation on Nested-loop Secondary Linear Machine Adapted to Linear Metro

With advantages of strong drive capability,nested-loop secondary linear machine(NLS-LM)has great potentiality in linear metro.For its secondary structure with multiple loops,it is difficult to calculate the electromagnetic thrust of NLS-LM reasonably.Hence,in this paper,one thrust calculation method is proposed considering variable loop inductance and transient loop current.Firstly,to establish the secondary winding function,the modeling domain is confined to a limited range,and the equivalent loop span is employed by analyzing the coupling relationship between primary and secondary.Then,in order to obtain the secondary flux density,the transient secondary current is solved based on the loop impedance and induced voltage.Finally,the electromagnetic thrust can be calculated reasonably by the given primary current sheet and the calculated secondary flux density.Comprehensive simulations and experiments have demonstrated the effectiveness of the proposed method.

Integrated Sliding Mode Velocity Control of Linear Permanent Magnet Synchronous Motor with Thrust Ripple Compensation

In this paper,a compound sliding mode velocity control scheme with a new exponential reaching law(NERL)with thrust ripple observation strategy is proposed to obtain a high performance velocity loop of the linear permanent magnet synchronous motor(LPMSM)control system.A sliding mode velocity controller based on NERL is firstly discussed to restrain chattering of the conventional exponential reaching law(CERL).Furthermore,the unavoidable thrust ripple caused by the special structure of linear motor will bring about velocity fluctuation and reduced control performance.Thus,a thrust ripple compensation strategy on the basis of extend Kalman filter(EKF)theory is proposed.The estimated thrust ripple will be introduced into the sliding mode velocity controller to optimize the control accuracy and robustness.The effectiveness of the proposal is validated with experimental results.

NAT技术的实验设计与仿真实现

文章讲述了使用NAT网络地址转换的背景意义、定义以及它的三种不同类型,并通过思科模拟器Cisco Packet Tracer Student仿真平台设计并验证实现了静态NAT、动态NAT及网络地址端口转换NAPT的综合组网实验方案。通过实验,加深了学生对NAT协议工作原理的认知和理解,提高了学生的组网能力,提升了教学效果。

Shati/Nat8l在神经系统中作用及机制的研究进展

Shati/Nat8l是具有N-乙酰基转移酶活性的N-乙酰转移酶-8样蛋白(Nat8l),在多个大脑区域表达,主要调节N-乙酰-L-天冬氨酸(NAA)的量,参与神经系统的神经发生、髓鞘形成、神经可塑性、神经营养因子调节、多巴胺能系统等的生理病理过程,与认知功能障碍等多种神经系统疾病的发病机制相关。本文综述Shati/Nat8l在神经系统中的作用及其分子机制,以期为神经系统疾病的诊治提供新方向。

基于冗余执行器的航空发动机推力快速响应控制方法研究

面向飞/推一体化控制需求,开展涡扇发动机推力快速响应控制研究,提出了适用于推力最小相位系统的冗余控制方案。研究内容包括:(1)借鉴阀位控制思想,提出在传统燃油流量(Wf)回路基础上增设尾喷口喉道面积(A8)回路,通过挖掘冗余执行器变化速率潜力,提高推力响应速度,降低了对于燃油泵快速作动的需求,同时该方法可以使喷口在推力响应过程逐渐回中,系统具备持续的推力快速调节能力;(2)分析了A8调节推力的物理机理,指出容腔压力动力学主导的部件间流量匹配过程使得A8对推力具有直馈作用,是提高推力响应速度的有效途径。基于发动机部件级模型的评估结果表明,本文提出的方法在执行机构存在速率限制的情况下,实现了推力10 rad/s闭环带宽和A8回中的设计目标,方法可行、有效。在推力响应过程中,A8直馈作用对推力变化的贡献度最高时超过50%。随着对于推力控制模式和推力响应速度的日趋关注,应对容腔内的压力动态过程给予充分的重视。