The Murine (C3H/He) Epidermal la^+ Dendritic Cells (la^+ DECs), Thy-1^+ Dendritic Cells (Thy-1^+DECs) and Aging

Identification and enumeration of both dendritic Ia^+ epi-dermal cells (Ia^+DECs) and dendritic Thy-1^+ epidermalcells (Thy-1^+DECa) from various parts of the body wereexamined by using epidermal sheets of C3H/He inbred miceof different age groups and indirect immunofluorescent tech-nique. A significant decline of both Ia^+DECs and Thy-1^+DECs in the mice of the aged group was demonstrated anddifferent densities and different distribution patterns betweenIa^+DECs and Thy-1^+DECs were obserged. These findingsmay imply that the decline of both Ia^+DECs and Thy-1^+DECs in the aged group may reflect the alterations of im-mune response in aging.

地塞米松和硫唑嘌呤对小鼠Thy-1^+树突状表皮细胞的影响

Thy-1阳性树突状表皮细胞(Thy-1^+dEC)是一种存在于正常小鼠表皮内的树突状细胞。应用抗Thy-1抗原的单克隆抗体和间接免疫荧光技术,我们证实:(1)外用地塞米松或硫唑嘌吟均可使小鼠Thy-1^+dEC密度明显降低(P<0.01);(2)系统应用不同剂量的地塞米松可使小鼠Thy-1^+dEC密度明显降低,(P<0.01),其降低程度与剂量呈直线负相关。