依达拉奉右莰醇联合丁苯酞氯化钠注射液对急性脑梗死患者血清单核细胞趋化蛋白-1、内皮素-1表达的影响

目的探讨依达拉奉右莰醇联合丁苯酞氯化钠注射液对急性脑梗死患者血清单核细胞趋化蛋白-1(MCP-1)、内皮素-1(ET-1)表达及动脉粥样硬化斑块的影响。方法选取河北省邯郸市中医院2020年12月至2022年8月收治的急性脑梗死患者140例,按随机数字表法分为联合组和对照组,各70例。两组患者均予丁苯酞氯化钠注射液,联合组患者加用依达拉奉右莰醇注射液,均治疗14 d。结果联合组总有效率为91.43%,显著高于对照组的68.57%(P<0.05)。治疗后,两组患者的颈动脉内中膜厚度、斑块面积、美国国立卫生研究院卒中量表(NIHSS)评分、改良Rankin量表(MRS)评分及血清MCP-1、ET-1、丙二醛、超氧化物歧化酶水平均显著降低(P<0.05),且联合组均显著低于对照组(P<0.05);联合组和对照组患者治疗期间不良反应发生率相当(12.86%比14.29%,P>0.05)。结论依达拉奉右莰醇联合丁苯酞氯化钠注射液治疗急性脑梗死的临床疗效良好,可有效改善患者的神经功能缺损情况、颈动脉粥样硬化斑块稳定性及内皮功能,降低氧化应激水平,提高生活质量,且安全性良好。

血必净注射液调控HMGB1/TLR4/NF-κB通路对脓毒症小鼠肺损伤的保护作用

目的 研究血必净注射液调控高迁移率族蛋白1(HMGB1)/Toll样受体4(TLR4)/核因子-κB(NF-κB)通路对脓毒症小鼠肺损伤的保护作用。方法 雄性C57BL/6小鼠随机分为对照组,模型组和低、中、高剂量血必净组,阴性对照(NC)组,NC+模型组,NC+高剂量血必净组,HMGB1+高剂量血必净组。采用盲肠结扎穿孔术建立脓毒症肺损伤模型,造模前给予NC慢病毒或HMGB1慢病毒尾静脉注射,造模当天给予血必净注射液(剂量5、10、15mL/kg)腹腔注射,2次/d,连续3 d,末次给药后24 h进行取材和检测。比较各组间肺组织病理改变,湿重(W)/干重(D)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量,裂解型Caspase-3、HMGB1、TLR4、NF-κB表达水平的差异。结果 模型组小鼠肺组织出现肺损伤的病理改变,W/D、TNF-α、IL-1β、IL-6、MDA的含量及裂解型Caspase-3、HMGB1、TLR4、NF-κB的表达水平均高于对照组,SOD的含量低于对照组(P<0.05)。不同剂量血必净组小鼠肺损伤的病理改变减轻,W/D、TNF-α、IL-1β、IL-6、MDA的含量及裂解型Caspase-3、HMGB1、TLR4、NF-κB的表达水平低于模型组,SOD的含量高于模型组(P<0.05)。HMGB1+高剂量血必净组小鼠肺损伤的病理改变加重,W/D、TNF-α、IL-1β、IL-6、MDA的含量及裂解型Caspase-3、HMGB1、TLR4、NF-κB的表达水平均高于NC+高剂量血必净组,SOD的含量低于NC+高剂量血必净组(P<0.05)。结论 血必净注射液对脓毒症小鼠肺损伤具有保护作用,并减轻炎症反应、氧化应激、细胞凋亡,其相关的分子机制为抑制HMGB1/TLR4/NF-κB通路。

玛湖凹陷玛页1井风城组页岩油地质甜点优选

玛湖凹陷风城组属于具有物源多、岩性复杂、整体含油、甜点分散等特征的混积型碱湖沉积,为了对页岩油进行高效勘探和开发,需要对页岩油地质甜点优选。以高压压汞、岩石热解等实验结果为基础,对玛页1井风城组储集层及其页岩油可动性等进行评价,构建页岩油地质甜点优选模型,评价页岩油地质甜点垂向分布特征。结果表明:孔隙度、总有机碳含量、脆性矿物含量和游离烃含量与100倍总有机碳含量之差分别是评价风城组储集层储集性能、含油性、脆性和页岩油可动性的参数;利用4个参数构建页岩油地质甜点优选模型,玛页1井风城组一类、二类和三类页岩油地质甜点的页岩油甜点因子分别为大于0.2823、0.0111~0.2823和小于0.0111;玛页1井风城组一类页岩油地质甜点主要分布在风二段上部和风三段,岩性以泥岩和白云质泥岩为主。

紫癜1号方治疗儿童过敏性紫癜性肾炎临床观察

目的:观察紫癜1号方对紫癜性肾炎(HSPN)患儿外周血嗜酸性粒细胞及其凋亡因子Fas蛋白表达的影响。方法:选取2019年10月1日—2020年12月30日于深圳市中医院门诊和住院部诊治的HSPN患儿60例,随机分为观察组和对照组,各30例。观察组予紫癜1号方加减及中药针剂治疗,对照组给予西医常规治疗,2组疗程均为12周。观察治疗前、治疗第0、4、8、12周各时间段的尿N-乙酰氨基葡萄糖苷酶(NAG)、尿微量白蛋白(mALB)、β2-微球蛋白(β2-MG),以及血嗜酸性粒细胞(EOS)、死亡诱导因子(Fas)等变化情况和疗效变化。结果:治疗第4、8周时,观察组总有效率分别为83.33%、96.67%,明显高于对照组,组间比较有显著性差异(P<0.05);第4、8周时,观察组降低患儿NAG的效果显著优于对照组(P<0.01);各个观察时点观察组降低β2-MG的效果均优于对照组(P<0.05);第4、12周时,观察组降低m ALB的效果优于对照组(P<0.05)。治疗第4周时,促进Fas抗原表达、降低EOS的效果对照组优于观察组,差异有统计学意义(P<0.01)。结论:紫癜1号方在早期能改善HSPN患儿临床症状,对改善早期肾损害指标起效更快,作用更持久。

红景天注射液对帕金森病患者治疗效果及血清DJ-1/Nrf2通路相关氧化应激指标的影响

目的 探究红景天注射液对帕金森病患者治疗效果及血清DJ-1/核因子相关因子2(Nrf2)通路相关氧化应激指标的影响。方法 前瞻性选取2021年6月至2022年6月来河北北方学院附属第一医院治疗的120例帕金森病患者作为研究对象,按照随机数字表法将患者分为研究组与对照组,每组各60例。对照组患者接受常规抗帕金森病治疗,研究组患者在此基础上联用红景天注射液治疗,连续治疗4周。比较两组患者的临床疗效,治疗前、治疗后4周的氧化应激指标[超氧化物歧化酶(SOD)、丙二醛、谷胱苷肽过氧化物酶(GSH-Px)、DJ-1蛋白、Nrf2],治疗前、治疗后4个月的帕金森病综合症状、生活质量情况,并观察两组患者不良反应发生情况。结果 治疗后,观察组患者的治疗有效率为96.61%,明显高于对照组(83.05%),差异有统计学意义(P<0.05)。治疗后4周,观察组患者血清SOD、GSH-Px、Nrf2水平分别为(44.27±2.19) U/mL、(44.12±6.72) U/L、(669.68±87.23) U/L,均明显高于对照组[(31.29±2.97) U/mL、(40.82±4.12) U/L、(602.09±52.31) U/L],而血清丙二醛、DJ-1蛋白水平分别为(2.27±0.86) nmol/mL、(7.08±2.19)μg/L,均明显低于对照组(3.69±1.15) nmol/mL、(10.92±1.29)μg/L,差异均有统计学意义(P<0.05)。治疗后4个月,观察组患者的UPDRS评分为(46.23±2.34)分,明显低于对照组[(63.28±1.93)分],差异有统计学意义(P<0.05)。治疗后4个月,观察组患者的生理功能、躯体功能、社会功能、心理状态、情感功能5个方面的评分分别为(73.12±5.22)、(78.23±2.34)、(81.23±2.13)、(88.29±3.91)、(86.39±3.23)分,均明显高于对照组[(53.13±6.48)、(59.28±4.92)、(69.24±5.91)、(71.23±2.03)、(71.13±3.25)分],差异均有统计学意义(P<0.05)。治疗后,观察组患者不良反应发生率为8.47%,明显低于对照组(23.73%),差异有统计学意义(P<0.05)。结论 在常规抗帕金森病治疗基础上,采用红景天注射液治疗帕金森病可明显提高患者的临床疗效,抑制DJ-1/Nrf2通路相关氧化应激反应,改善帕金森病症状,提高患者生活质量,降低不良反应发生率。

注射用乌司他丁联合纤维支气管镜吸痰治疗重症肺炎的疗效及对患者血清sTREM-1、sCD163的影响

目的:探究注射用乌司他丁联合纤维支气管镜吸痰治疗重症肺炎的疗效及对患者血清sTREM-1、sCD163的影响。方法:选取2020年6月至2023年6月滨州医学院附属医院收治的重症肺炎患者为研究对象。将符合排纳标准的92例重症肺炎患者随机分为对照组(n=46)和联合组(n=46)。对照组在常规治疗基础上行纤维支气管镜吸痰,联合组在对照组的基础上加用乌司他丁。观察两组患者的炎性因子水平(PCT、TNF-α、CRP)及sTREM-1、sCD163水平、血气分析指标(血氧分压和氧合指数)、临床疗效。结果:(1)治疗前,两组炎性因子水平对比(P>0.05),治疗后,联合组的炎性因子水平(TNF-α、CRP、PCT)均低于对照组(P<0.05)。(2)治疗前,两组血清sTREM-1、sCDl63水平对比(P>0.05),治疗后,联合组的血清sTREM-1、sCDl63水平均低于对照组(P<0.05)。(3)治疗前,两组血氧分压、氧合指数对比(P>0.05),治疗后,联合组的血氧分压、氧合指数均低于对照组(P<0.05)。(4)联合组的有效率为95.65%高于对照组的82.61%(P<0.05)。结论:注射用乌司他丁联合纤维支气管镜吸痰可有效改善重症肺炎患者的炎性因子水平、血气分析指标、临床疗效。

Evaluation of the efficacy and safety of endoscopic band ligation in the treatment of bleeding from mild to moderate gastric varices type 1

BACKGROUND According to practice guidelines,endoscopic band ligation(EBL)and endoscopic tissue adhesive injection(TAI)are recommended for treating bleeding from esophagogastric varices.However,EBL and TAI are known to cause serious complications,such as hemorrhage from dislodged ligature rings caused by EBL and hemorrhage from operation-related ulcers resulting from TAI.However,the optimal therapy for mild to moderate type 1 gastric variceal hemorrhage(GOV1)has not been determined.Therefore,the aim of this study was to discover an individualized treatment for mild to moderate GOV1.AIM To compare the efficacy,safety and costs of EBL and TAI for the treatment of mild and moderate GOV1.METHODS A clinical analysis of the data retrieved from patients with mild or moderate GOV1 gastric varices who were treated under endoscopy was also conducted.Patients were allocated to an EBL group or an endoscopic TAI group.The differences in the incidence of varicose relief,operative time,operation success rate,mortality rate within 6 wk,rebleeding rate,6-wk operation-related ulcer healing rate,complication rate and average operation cost were compared between the two groups of patients.RESULTS The total effective rate of the two treatments was similar,but the efficacy of EBL(66.7%)was markedly better than that of TAI(39.2%)(P<0.05).The operation success rate in both groups was 100%,and the 6-wk mortality rate in both groups was 0%.The average operative time(26 min)in the EBL group was significantly shorter than that in the TAI group(46 min)(P<0.01).The rate of delayed postoperative rebleeding in the EBL group was significantly lower than that in the TAI group(11.8%vs 45.1%)(P<0.01).At 6 wk after the operation,the healing rate of operation-related ulcers in the EBL group was 80.4%,which was significantly greater than that in the TAI group(35.3%)(P<0.01).The incidence of postoperative complications in the two groups was similar.The average cost and other related economic factors were greater for the EBL than for the TAI(P<0.01).CONCLUSION For mild to moderate GOV1,patients with EBL had a greater one-time varix eradication rate,a greater 6-wk operation-related ulcer healing rate,a lower delayed rebleeding rate and a lower cost than patients with TAI.

HIV-1 Transmission among Injecting Drug Users is Principally Derived from Local Circulating Strains in Guangxi, China

Objective The mode of human immunodeficiency virus(HIV) transmission via injection drug use(IDU)still exists, and the recent shift in IDU-related transmission of HIV infection is largely unknown. The purpose of this study was to analyze the spatiotemporal sources and dynamics of HIV-1 transmission through IDU in Guangxi.Methods We performed a molecular epidemiological investigation of infections across Guangxi from2009 to 2019. Phylogenetic and Bayesian time-geographic analyses of HIV-1 sequences were performed to confirm the characteristics of transmission between IDUs in combination with epidemiological data.Results Among the 535 subjects, CRF08_BC(57.4%), CRF01_AE(28.4%), and CRF07_BC(10.7%) were the top 3 HIV strains;72.6% of infections were linked to other provinces in the transmission network;93.6% of sequence-transmitted strains were locally endemic, with the rest coming from other provinces,predominantly Guangdong and Yunnan;92.1% of the HIV transmission among people who inject drugs tended to be transmitted between HIV-positive IDUs.Conclusion HIV recombinants were high diversity, and circulating local strains were the transmission sources among IDUs in Guangxi. However, there were still cases of IDUs linked to other provinces.Coverage of traditional prevention strategies should be expanded, and inter-provincial collaboration between Guangxi, Yunnan, and Guangdong provinces should be strengthened.

Effect of Salvia Miltiorrhiza Injection on Blood Pressure and Cardiac Function in Rats with Gestational Hypertension and Preeclampsia

Objective: This study is to observe the effects of Salvia miltiorrhiza injection on blood pressure and cardiac function in rats with pregnancy-induced hypertension and preeclampsia. Methodology: Syncytiotrophoblast microvilli (stbm) and l-arginine nitrosyl methyl ester were screened out via caudal vein injection. Twenty gestational hypertension-preeclampsia model SD (Sprague Dawley) rats successfully induced by L-NAME (L-arginine Nitrosyl methyl ester) were randomly divided into 2 groups (model group and Danshen injection group, n = 10). Then another 10 normal pregnant SD rats without model were selected as blank control group. The Salvia miltiorrhiza injection group was given Salvia miltiorrhiza injection (0.5 g?kg?1?d?1) through tail vein, and the control group and model group were given equal volume of normal saline through tail vein injection. All three groups were treated by tail vein injection once a day (d) for 7 days. After treatment, heart rate (HR), Systolic pressure (SP), diastolic pressure (DP) and mean arterial pressure (MAP) were measured by tail artery. Left ventricular end-diastolic diameter (LVDd) and Left ventricular end systolic diameter (LVDs) were recorded by echocardiography. Left ventricular end diastolic pressure (LVEDP), left ventricular systolic pressure (LVSP), left ventricular ejection fraction (left ventricular ejection) fraction, LVEF) and the maximum rate of increase/decrease of left ventricular pressure during isovolemic systole (+dp/dtmax/?dp/dtmax);Endothelin-1 (ET-1) levels in rat tail vein blood were detected by ELISA. Results: SP, DP, MP, HR, LVSP, LVDs and ?dp/dtmaxx were all decreased, plasma ET-1 expression was low, and LVDd, LVEDP, LVEF, and +dp/dtmax were all increased in the Salvia miltiorroot injection group, with statistical significance compared to the model group (p Conclusion: Salvia miltiorrhiza injection can improve the cardiac function and reduce blood pressure in rats with pregnancy-induced hypertension and preeclampsia, and the mechanism may be related to alleviating systemic arteriolar spasm by regulating ET-1 level.

Development of SA-533 Type B CL. 1+SA-240 Type 304L roll-bonded clad steel plate for safety injection tank of CAP1400 nuclear power plant

Aiming to meet the demand of the country＇ s nuclear demonstration project on the CAP1400 nuclear power plant, Baosteel uses the roll-bonding technology and develops the SA-533 Type B CL. 1 ＋ SA-240 Type 304L high-strength and high-toughness clad steel plate with a shear strength of over 310 MPa for the nuclear power plant＇ s safety injection tank. The properties of the quenched and tempered and the simulated post-weld heat treatment states are systematically studied herein through a comprehensive inspection and evaluation of the composition,microstructure,and properties of the clad steel plate. The results show that the bonding interface has high shear strength and that the base metal has high strength and good toughness at low temperatures. Hence, the performance fully meets the technical requirements of the CAP1400 nuclear power plant＇ s safety injection tank in the country＇ s nuclear demonstration project. The roll-bonded clad steel plate can be used to manufacture the safety injection tank of the CAP1400 nuclear power plant.

舒肝宁注射液的^(1)H-NMR指纹图谱分析

目的对舒肝宁注射液的^(1)H-NMR指纹图谱进行分析,为其质量控制标准技术升级提供参考依据。方法使用Noesygppr1d脉冲序列[recycle delay(RD)-90°-t 1-90°-t_(m)-90°-ACQ]采集1H-NMR谱图,质子共振频率为600.13 MHz,实验温度为298 K,通过方法学考察验证分析方法可靠性,采用相关系数法和夹角余弦法评价不同批次舒肝宁注射液样品的相似度。结果对舒肝宁注射液的^(1)H-NMR指纹图谱进行分析,共鉴定出88种化学成分,且不同批次的舒肝宁注射液具有较高的相似度。结论建立了一种基于^(1)H-NMR的舒肝宁注射液质量控制方法,可为舒肝宁注射液技术升级提供参考依据。

疏血通注射液辅助治疗对老年缺血性脑卒中患者认知功能及TGF-β_(1)、Smad1的影响

目的:探讨疏血通注射液辅助治疗对老年缺血性脑卒中患者认知功能及转化生长因子-β_(1)(TGF-β_(1))、Smad同源物1(Smad1)的影响。方法:选择2021年4月—2023年4月昆山市锦溪人民医院收治的82例老年缺血性脑卒中患者作为研究对象。将随机分为观察组、对照组,各41例。对照组采用常规治疗,观察组在对照组基础上采用疏血通注射液辅助治疗。比较两组认知功能、TGF-β_(1)、Smad1、自理能力和神经功能、不良反应发生率。结果:治疗后,两组简易精神状态检查量表(MMSE)、蒙特利尔认知评估量表(MoCA)评分高于治疗前,临床痴呆评定量表(CDR)评分低于治疗前,且观察组MMSE、MoCA评分高于对照组,CDR评分低于对照组,差异有统计学意义(P<0.05)。治疗后,两组TGF-β_(1)低于治疗前,Smad1高于治疗前,且观察组TGF-β_(1)低于对照组,Smad1高于对照组,差异有统计学意义(P<0.05)。治疗后,两组日常生活能力量表(ADL)评分高于治疗前,美国国立卫生研究院卒中量表(NIHSS)评分低于治疗前,且观察组ADL评分高于对照组,NHISS评分低于对照组,差异有统计学意义(P<0.05)。观察组不良反应总发生率为7.32%,低于对照组的14.63%,但两组间比较,差异无统计学意义(P>0.05)。结论:疏血通注射液辅助治疗能够有效改善老年缺血性脑卒中患者的认知功能、TGF-β_(1)、Smad1,提高患者自理能力及神经功能,具有较高的安全性。

Optic diagnosis of neutral beam injection on HL-1M

During the operation of a high-power neutral beam injection (NBI) system on theHL-1M tokamak, an optical diagnostic means using CCD camera was developed to characterize theNBI performance. The vacuum valve opening process and NBI period in the HL-1M experimentwere displayed by a lot of photos taken with this means. Thus, the Hα emission profiles of theneutral beam (NB) and its interaction with plasma were given. Finally, the reason possible forplasma breakdown during NBI mode Ⅱ discharge was investigated. Therefore, this in-situ diagnosiscan provide more information of the NBI.

Fuelling and Diagnostics with Pellet Injection in the HL-1M Tokamak

The pellet injection experiments for fuelling and diagnostics have been carried out on the HL-1M tokamak. The eight-pellet injector was installed on HL-1M. A reliable monitordetector and camera system was set up to take initial pellet photographs and measure the initial pellet speed and size. High fuelling efficiency of 60 % - 100 % and a density profile with a peaking factor of 1.8 - 2.0 were obtained. The maximum density close to 10^14/cm^3 in HL-1M was achieved with newly optimized combined fuelling techniques. Two typical models of pellet ablation have been utilized for simulative calculation of the ablation rates in HL-1M. In comparison with the distribution of the measured Hα emission intensity from the digital data of the CCD camera, the experimental result seems more optimistic for core fuelling than theoretical predictions by the two models. The safety factor profile q（r） has been extracted from the information provided by the CCD camera during the pellet injection. The reliability of the measured results depends mainly on the calibration of the imaging space position. Based on the calibration, the measured q-profile becomes more reasonable than those published previously for the same shot number and same photograph.

酚磺乙胺注射液与白眉蛇毒血凝酶、维生素K_(1)联合在新生儿便血治疗中的价值

目的:分析酚磺乙胺注射液与白眉蛇毒血凝酶、维生素K_(1)联合在新生儿便血治疗中的价值。方法:选取2019年10月—2022年12月江南大学附属儿童医院收治的90例新生儿便血患儿作为研究对象,按随机数表法分成两组,对照组实施酚磺乙胺注射液治疗,观察组实施酚磺乙胺注射液与白眉蛇毒血凝酶及维生素K_(1)治疗。比较两组临床疗效、不良反应发生率、炎症因子水平。结果:观察组总有效率高于对照组,差异有统计学意义(P<0.05)。两组不良反应发生率对比,差异无统计学意义(P>0.05)。观察组白细胞介素-6(interleukin-6,IL-6)、C反应蛋白(C-reactive protein,CRP)低于对照组,白细胞介素-10(interleukin-10,IL-10)高于对照组,差异有统计学意义(P<0.05)。结论:酚磺乙胺注射液与白眉蛇毒血凝酶、维生素K_(1)联合在新生儿便血治疗中可行性较高,可以改善炎症状态,提升治疗效果和安全性。

Effects of astragalus injection on the skin lesion degree and Caspase-14,SOCS1 and STAT3 levels of psoriasis model in Balb/c nude mice

Objective: To investigate the effect of Astragalus Injection on the Skin Lesion Degree and Caspase-14, SOCS1 and STAT3 Levels of Psoriasis Model in Balb/c Nude Mice. Methods:Sixty Balb/c nude mice were randomly divided into groups A, B and C with 20 mice in each group. Group A mice were used as blank control, group B mice as model group and group C mice as treatment group. The PASI score of psoriasis, skin thickness, inflammatory factors, serum levels of Caspase-14, SOCS1 and STAT3 in three groups of mice were analyzed after 2 weeks of treatment. Result: After treatment, the P ASI score of group B was significantly higher than that of group C, with statistical significance (P < 0.05);there was statistical significance in the measurements of lesion skin of three groups of mice after treatment (P <0.05). Compared with the blank control group, the thickness of lesion skin in group B and C was significantly higher, and the thickness of lesion skin in treatment group was significantly lower than that in control group (P < 0.05). Compared with the blank control group, the inflammatory factors IL-17, IL-22 and IL-23 in the B and C groups were significantly increased, and the inflammatory factors IL-17, IL-22 and IL-23 in the treatment group were significantly lower than those in the model group. The levels of serum C aspase-14, SOCS1 and STAT3 in three groups of mice were significantly different after treatment (P < 0.05). Compared with the blank control group, the levels of serum C aspase-14 and SOCS1 in B and C groups were significantly lower and the levels of STAT3 were significantly higher, and the levels of inflammatory factors aspase-14 and SO in treatment group were significantly higher than those in control group. The level of CS1 was significantly lower than that of model group, and the level of STAT3 was significantly higher than that of model group (P <0.05). Conclusion: Astragalus membranaceus injection can effectively improve the degree of psoriasis in Balb/c nude mice. Its possible mechanism is that it can decrease the expression of Caspase-14 and SOCS1, reduce the degree of keratosis in the lesion site of mice, improve the local surface hyperplasia, increase the level of STAT3 and enhance the level of local cell proliferation, which is of positive significance for the rehabilitation of psoriasis.

顶空-气相色谱法同时测定维生素B_(1)片中9种溶剂残留量

目的 建立维生素B_(1)片中甲醇、乙醇、丙酮、甲酸乙酯、乙腈、正己烷、乙酸乙酯、四氢呋喃和正庚烷9种残留有机溶剂气相色谱分离测定方法。方法 采用顶空毛细管气相色谱法,6%(V/V)氰丙基苯基-94%(V/V)二甲基聚硅氧烷(DB-624)为固定液的毛细管柱(30 m×0.530 mm×3.00 μm),氢火焰离子化检测器(FID),载气为氮气,溶剂为N,N-二甲基甲酰胺(DMF)∶水(1∶1),按外标法计算残留溶剂的含量。结果 9种有机溶剂均能有效分离,峰响应与浓度均呈良好线性关系,相关系数r均大于0.999 64;平均回收率在92.2%~111.7%(n=9);精密度RSD值在0.3%~1.0%(n=6)。结论 该方法分离效果好、灵敏、可靠、准确,适用于维生素B1片中9种溶剂残留量的同时测定。

Clinical efficacy of Danshen injection in combined with local injection of triamcinolone acetonide in the treatment of oral submucous fibrosis and the effect on serum TGF-β1 and IL-6

Objective:To explore the clinical efficacy of Danshen injection in combined with local injection of triamcinolone acetonide in the treatment of oral submucous fibrosis (OSF) and the effect on serum TGF-β1 and IL-6.Methods:A total of 70 patients with OSF who were admitted in our hospital from October, 2015 to October, 2016 were included in the study and randomized into the observation group and the control group (n=35). The patients in the control group were given multipoint injection of triamcinolone acetonide (40 mg/time) in the basal region of oral mucosal regions. On this basis, the patients in the observation group were given additional Danshen injection (4 mL/time), 1 time/week, for 12 times. VAS was used for evaluation. The oral mucosa lesion area and mouth opening were measured. The morning fasting peripheral venous blood before and after treatment in the two groups was collected. The blood rheometer was used to detect the whole blood viscosity [high shear (HS), moderate shear (MS), and low shear (LS)], plasma viscosity (PV), and fibrinogen (FIB). ELISA was used to detect the serum TGF-β1 and IL-6 levels.Results: VAS score in the observation group was significantly reduced (P<0.05), mucosal lesion area was significantly less than that in the control group (P<0.05), and mouth opening was significantly greater than that in the control group (P<0.05). LS, MS, PV, and FIB after treatment in the observation group were significantly reduced when compared with before treatment (P<0.05), while LS, MS, HS, PV, and FIB after treatment in the control group were not significantly changed (P>0.05). TGF-β1 and IL-6 levels after treatment in the observation group were significantly lower than those in the control group (P<0.05).Conclusions: Danshen injection in combined with local injection of triamcinolone acetonide in the treatment of OSF can effectively reduce the serum TGF-β1 and IL-6 levels, inhibit the connective tissue fibrosis, and improve the pain, oral mucosal lesion area, and mouth opening, with a satisfactory clinical effect.

依达拉奉右莰醇对阿替普酶溶栓后MMP-9、ICAM-1、ET-1的影响及临床疗效分析

目的 探讨依达拉奉右莰醇对阿替普酶溶栓后血清细胞间黏附分子(ICAM-1)、内皮素-1(ET-1)、血浆基质金属蛋白酶(MMP-9)水平的影响及临床疗效。方法 选取湖南省脑科医院2021年12月-2022年12月急性缺血性脑卒中(AIS)患者80例,分对照组和观察组。对照组:阿替普酶溶栓治疗后标准化治疗;观察组:阿替普酶溶栓治疗后标准化治疗+依达拉奉右莰醇注射液,疗程2 w。评估临床疗效、临床症状评分、美国国立卫生研究所卒中量表(NIHSS)评分、ALBERTA卒中项目早期CT量表(ASPECTS)评分、Barthel (BI)指数评分,检测血液中MMP-9、ET-1、ICAM-1水平变化。结果 观察组有效率高于对照组(P<0.05)。治疗后,2组患者NIHSS评分、临床症状评分均降低(P<0.01),ASPECTS、BI指数评分均升高(P<0.01),血清MMP-9、ET-1、ICAM-1含量水平均降低(P<0.01),以观察组变化更明显(P <0.01)。结论 依达拉奉右莰醇注射液能明显抑制阿替普酶溶栓后患者的MMP-9、ET-1、ICAM-1细胞因子的表达,改善AIS超早期阿替普酶静脉溶栓患者临床症状,疗效确切。

CLINICAL OBSERVATION ON 60 CASES OF BENIGN HYPERPLASIA OF PROSTATE TREATED BY INJECTION OF "CHUAN-SHEN-TONG" INJECTIOINTO HUIYIN (CV1)

Objective: To observe the therapeutic e ffect of injection of "Chuan-Shen-Tong" Injectio (川参通注射液) at Huiyin (会阴 CV 1) on benign hyperplasia of prostate . Methods: 60 cases of benign hyperplasia of prostate patients were evenly randomized into treatment group and control group. Those of treatment group were treated with injection o f "Chuan-Shen-Tong" Injectio (composed of Rhizoma Ligustici Chuanxiong, Rad ix Salviae Miltiorrhizae, etc. for relieving hyperplasis of prostate, 4 mL/acupoin t) into Huiyin (CV 1) and the other 60 cases of control group were treated by oral administration of Hytrin (1 mg/d in the 1 st week and increasing 1 mg/3 d, 4 mg at m ost). After 28 days’ treatment, their therapeutic effects were compared. Result s: After treatment, of each 60 cases in treatment and control groups, 11 (18.3%) and 11 (11.7%) were controlled in their symptoms, 25 (41.7%) and 16 (26.7%) had marked improvement, 18 (30.0%) and 17 (28.3%) had improvement, and 6 (10.0%) an d 20 (33.3%) failed, with the effective rate and the markedly effective rate bei ng 90.0% and 66.7% in the treatment group and 66.7% and 38.3% in the control gro up respectively. The therapeutic effect of treatment group was significantly sup erior to that of control group (P<0.01 and P< 0.05). Conclusion: The therapeutic effect of point-injection of "Chuan-Shen-Tong" for benign hyperplasia of p rostate is better than that of oral administration of Hytrin.