Herbal medicine from natural resources plays an important role as antibacterial and antioxidant agents. The present investigation was designed to evaluate the antibacterial and antioxidant properties of thyme (Thymus ...Herbal medicine from natural resources plays an important role as antibacterial and antioxidant agents. The present investigation was designed to evaluate the antibacterial and antioxidant properties of thyme (Thymus vulgaris L.) essential oil (TEO) and/or chitosan (CH) in vitro. Results indicated that TEO exhibited high radical scavenging activity (RSA) toward DPPH, ABTS, linoleic acid deterioration and iron chelation activity. TEO exhibited high amount of total phenolic compounds (TPC) related to its terpenes. The TPC of TEO was 177.3 mg GAE g-1 demonstrated 149.8 μmol of TE g-1 DPPH-RSA and 192.4 μmol of TE g-1 ABTS-RSA. The antioxidant capacity of TEO exhibited 68.9% reduction when evaluated by β-carotene bleaching assay. The reducing power activity related to iron chelation was 142.8 μmol of AAE g-1. The TEO exhibited a high content of Thymol (41.04%) as major compound over 14 identified components by GC-MS analysis followed by 1,8-Cineole (14.26%), γ-Terpinene (12.06%), p-Cymene (10.50%) and α-Terpinene (9.22%). TEO exhibited antimicrobial activity in vitro and MIC noticed that TEO was efficiently affected pathogens in vitro. Indeed, CH exhibited negligible or very low antimicrobial activity. In conclusion, both investigated TEO and TEO-CHmix have strong antibacterial activity against many pathogenic bacteria and need exploitation as an alternative source of natural antibacterial and antioxidant agents for potential applications.展开更多
Objective: To focus on the analysis of chemical constituents of the Thymus vulgaris L.(locally known as "Zaitra" or "Za'atar"; Family: Lamiaceae) which is available in the market of Saudi Arabi...Objective: To focus on the analysis of chemical constituents of the Thymus vulgaris L.(locally known as "Zaitra" or "Za'atar"; Family: Lamiaceae) which is available in the market of Saudi Arabia.Methods: The Zaitra oil was analyzed by gas chromatography-mass spectrometry. The mass spectra were compared with the standard spectra available in National Institute Standard and Technique library.Results: The results indicated that the Thymus oil is composed of many chemical compounds including a pinene, thymol and caryophyllene which are biologically active and also used in various diseases.Conclusions: It can be concluded that the Thymus vulgaris due to presence of many bioactive compounds can be used as a new potential source of medicine for the treatment of various types of illness.展开更多
Currently, there are no data in the literature on the topical treatment with Thymus vulgaris essential oil (EO) in bacterial skin infections of dogs. This study evaluates the effectiveness of Thymus vulgaris EO for to...Currently, there are no data in the literature on the topical treatment with Thymus vulgaris essential oil (EO) in bacterial skin infections of dogs. This study evaluates the effectiveness of Thymus vulgaris EO for topical use in dogs with skin 18 half-breed dogs, affected by skin infections housed in a rescue shelter were studied. The bacteria isolated from these dogs were identified by MALDI-TOF-MS. The susceptibility of the isolated microorganisms to Thymus vulgaris L. EO was estimated in vitro by bacteriological test (CLSI 2015), in comparison to some antimicrobials drugs (amoxicillin-clavulanic acid, doxycy-cline, thiamphenicol and marbofloxacin) and to Citrus bergamia EO. The dogs, divided in two groups at random, were treated topically for 7 days with Thymus vulgaris L. EO (Group 1: n. 10 animals) and Citrus bergamia Risso e Poiteau EO (Group 2: n. 8 animals) respectively. The bacteria isolated were: Staphylococcus pseudintermedius (7 samples), Staphylococcus sciuri (4), ESBL Escherichia coli (3) and Proteus mirabilis (4). In all animals treated with Thymus vulgaris EO, the clinical signs decreased rapidly within 5 days from the administration, with complete remission 7 days after the treatment. No bacterial growth was observed from skin swabs after 7 days of treatment. None of the treated animals showed local or general side effects. The use of Thymus vulgaris EO could be a possible alternative or additional treatment to antibiotics in dermatological infections, particularly in cases refractory to conventional therapy.展开更多
[Objective] This study aimed to compare and analyze the chemical compositions, the relative contents and the antibacterial activity of the essential oils extracted from the tissue culture seedlings, the aseptic seedli...[Objective] This study aimed to compare and analyze the chemical compositions, the relative contents and the antibacterial activity of the essential oils extracted from the tissue culture seedlings, the aseptic seedlings and the common seedlings from seeds of Thymus vulgaris. [Method] The essential oil was extracted by steam distillation; the chemical compositions and their relative contents were analyzed by gas chromatography-mass spectrometry （GC/MS） and peak area normalization method; and the antimicrobial activity was tested by filter paper method. [Result] A total of 54 compositions were identified from these three samples, they had similar main compositions： thymol, m-Cymene, terpinene and so on, and these compositions were slightly different in contents. In addition, the essential oils from these three samples exhibited obvious antibacterial effect against Escherichia coli and Staphylococcus aureus, in which the antibacterial activity against S. aureus was stronger than against E. coli. [Conclusion] It is possible to produce essential oil by tissue culture technology; the main compositions and antibacterial activity of the oils remained almost the same.展开更多
文摘Herbal medicine from natural resources plays an important role as antibacterial and antioxidant agents. The present investigation was designed to evaluate the antibacterial and antioxidant properties of thyme (Thymus vulgaris L.) essential oil (TEO) and/or chitosan (CH) in vitro. Results indicated that TEO exhibited high radical scavenging activity (RSA) toward DPPH, ABTS, linoleic acid deterioration and iron chelation activity. TEO exhibited high amount of total phenolic compounds (TPC) related to its terpenes. The TPC of TEO was 177.3 mg GAE g-1 demonstrated 149.8 μmol of TE g-1 DPPH-RSA and 192.4 μmol of TE g-1 ABTS-RSA. The antioxidant capacity of TEO exhibited 68.9% reduction when evaluated by β-carotene bleaching assay. The reducing power activity related to iron chelation was 142.8 μmol of AAE g-1. The TEO exhibited a high content of Thymol (41.04%) as major compound over 14 identified components by GC-MS analysis followed by 1,8-Cineole (14.26%), γ-Terpinene (12.06%), p-Cymene (10.50%) and α-Terpinene (9.22%). TEO exhibited antimicrobial activity in vitro and MIC noticed that TEO was efficiently affected pathogens in vitro. Indeed, CH exhibited negligible or very low antimicrobial activity. In conclusion, both investigated TEO and TEO-CHmix have strong antibacterial activity against many pathogenic bacteria and need exploitation as an alternative source of natural antibacterial and antioxidant agents for potential applications.
基金Supported by Prince Sultan Military Medical City,Riyadh,Saudi Arabia(Grant Number#APX-2014-034)
文摘Objective: To focus on the analysis of chemical constituents of the Thymus vulgaris L.(locally known as "Zaitra" or "Za'atar"; Family: Lamiaceae) which is available in the market of Saudi Arabia.Methods: The Zaitra oil was analyzed by gas chromatography-mass spectrometry. The mass spectra were compared with the standard spectra available in National Institute Standard and Technique library.Results: The results indicated that the Thymus oil is composed of many chemical compounds including a pinene, thymol and caryophyllene which are biologically active and also used in various diseases.Conclusions: It can be concluded that the Thymus vulgaris due to presence of many bioactive compounds can be used as a new potential source of medicine for the treatment of various types of illness.
文摘Currently, there are no data in the literature on the topical treatment with Thymus vulgaris essential oil (EO) in bacterial skin infections of dogs. This study evaluates the effectiveness of Thymus vulgaris EO for topical use in dogs with skin 18 half-breed dogs, affected by skin infections housed in a rescue shelter were studied. The bacteria isolated from these dogs were identified by MALDI-TOF-MS. The susceptibility of the isolated microorganisms to Thymus vulgaris L. EO was estimated in vitro by bacteriological test (CLSI 2015), in comparison to some antimicrobials drugs (amoxicillin-clavulanic acid, doxycy-cline, thiamphenicol and marbofloxacin) and to Citrus bergamia EO. The dogs, divided in two groups at random, were treated topically for 7 days with Thymus vulgaris L. EO (Group 1: n. 10 animals) and Citrus bergamia Risso e Poiteau EO (Group 2: n. 8 animals) respectively. The bacteria isolated were: Staphylococcus pseudintermedius (7 samples), Staphylococcus sciuri (4), ESBL Escherichia coli (3) and Proteus mirabilis (4). In all animals treated with Thymus vulgaris EO, the clinical signs decreased rapidly within 5 days from the administration, with complete remission 7 days after the treatment. No bacterial growth was observed from skin swabs after 7 days of treatment. None of the treated animals showed local or general side effects. The use of Thymus vulgaris EO could be a possible alternative or additional treatment to antibiotics in dermatological infections, particularly in cases refractory to conventional therapy.
基金Supported by Key Science and Technology Project of Shenzhen City(2109k3070009)National Key Technology Research and Development Program during the 12 th Five-Year Plan Period (2011BAD18B02)~~
文摘[Objective] This study aimed to compare and analyze the chemical compositions, the relative contents and the antibacterial activity of the essential oils extracted from the tissue culture seedlings, the aseptic seedlings and the common seedlings from seeds of Thymus vulgaris. [Method] The essential oil was extracted by steam distillation; the chemical compositions and their relative contents were analyzed by gas chromatography-mass spectrometry （GC/MS） and peak area normalization method; and the antimicrobial activity was tested by filter paper method. [Result] A total of 54 compositions were identified from these three samples, they had similar main compositions： thymol, m-Cymene, terpinene and so on, and these compositions were slightly different in contents. In addition, the essential oils from these three samples exhibited obvious antibacterial effect against Escherichia coli and Staphylococcus aureus, in which the antibacterial activity against S. aureus was stronger than against E. coli. [Conclusion] It is possible to produce essential oil by tissue culture technology; the main compositions and antibacterial activity of the oils remained almost the same.
