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Expression of Thyroid Transcription Factor-1(TTF-1)in Lung Carcinomas and Its Correlations with Apoptosis and Angiogenesis 被引量:3
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作者 Xiaoyan Bai Hong Shen Chunhui Zhou Hao Wang 《Chinese Journal of Clinical Oncology》 CSCD 2009年第1期16-20,共5页
OBJECTIVE To investigate the correlations between the expression of thyroid transcription factor-1 (TTF-1) and apoptosis and angiogenesis in lung carcinomas. METHODS A 829 microarray of the paraffin tissue chips was... OBJECTIVE To investigate the correlations between the expression of thyroid transcription factor-1 (TTF-1) and apoptosis and angiogenesis in lung carcinomas. METHODS A 829 microarray of the paraffin tissue chips was constructed, which contained 196 lung carcinomas, 10 normal lung tissues, and 1 muscular tissue. Terminal deoxynucleotidyl transferase mediated nick end labeling (TUNEL) and immunohistochemical SP method were used to detect apoptosis and expression of TTF-1 and CD34 in different types of lung carcinomas. A Leica Q500 MC image analysis system was used to measure and calculate TTF-1 positive unit (PU), apoptotic index (AI) and microvessel density (MVD). RESULTS AI of lung small cell carcinoma and large cell carcinoma were smaller than those of lung adenocarcinoma and squamous cell carcinoma (P = 0.000). AI of lung carcinomas with lymph node metastases was smaller than that of those without (P = 0.039). AI of lung carcinomas in TNM stage I-IV was smaller than that in stage I (P = 0.008). The PU of the TTF-1 was negatively correlated with AI in small cell lung carcinoma (r = -0.752, P = 0.000). MVD of lung carcinomas without lymph node metastases was smaller than that of those with lymph node metastasis (P = 0.031). MVD of lung carcinomas in TNM stage I was smaller than that in stage I-IV (P = 0.040). The PU of TTF-1 was positively correlated with MVD in lung adenocarcinoma (r = 0.708, P = 0.000). CONCLUSION There is a negative correlation between TTF-1 PU and AI in small cell lung carcinoma. TTF-1 PU and AI may be correlated with each other. There is a positive correlation between TTF-1 PU and MVD in lung adenocarcinoma. TTF-1 may induce the development of lung adenocarcinoma by inducing tumor angiogenesis. 展开更多
关键词 lung carcinoma thyroid transcription factor-1 tissue chip APOPTOSIS angiogenesis.
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Reliability of a Tissue Microarray in Detecting Thyroid Transcription Factor-1 Protein in Lung Carcinomas 被引量:1
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作者 Xiaoyan Bai Hong Shen 《Chinese Journal of Clinical Oncology》 CSCD 2007年第1期9-15,共7页
OBJECTIVE To compare the expression of the thyroid transcription factor-1 (TTF-1) in human normal adult type Ⅱ alveolar epithelial cells, embryonic pneumocytes and cancer cells of lung carcinoma and metastatic lymp... OBJECTIVE To compare the expression of the thyroid transcription factor-1 (TTF-1) in human normal adult type Ⅱ alveolar epithelial cells, embryonic pneumocytes and cancer cells of lung carcinoma and metastatic lymph nodes using a tissue microarray (TMA) along with paired conventional full sections, and to investigate the reliability of tissue microarrays in detecting protein expression in lung carcinoma. METHODS A lung carcinoma TMA including 765 cores was constructed. TTF-1 protein expression in both TMA and paired conventional full sections were detected by the immunohistochemical SP method using a monoclonal antibody to TTF-1. A PU (Positive Unit) of TTF-1 protein was assessed quantitatively by the Leica Q500MC image analysis system with results from the paired conventional full sections as controls. RESULTS There was no significance between TMA and paired conventional full sections in TTF-1 expression in different nuclei of the lung tissue. CONCLUSION TTF-1 protein expression in lung carcinoma detected by TMA was highly concordant with that of paired full sections. TMA is a reliable method in detecting protein expression. 展开更多
关键词 lung carcinoma transcription factor-1(TTF-1 liability. tissue microarray thyroid immunohistochemistry reliability.
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肝细胞癌患者癌组织TTF-1、p53、p63表达变化及其对术后生存的影响
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作者 罗珂 丁莉 刘美 《实用肝脏病杂志》 CAS 2024年第3期434-437,共4页
目的探讨肝细胞癌(HCC)患者癌组织甲状腺转录因子(TTF)-1、p53和p63表达变化及其对术后生存的影响。方法2019年1月~2021年12月我院收治的HCC患者56例,均行肝内肿瘤切除术治疗,术后随访2年。采用免疫组化法检测癌组织和癌旁组织TTF-1、p5... 目的探讨肝细胞癌(HCC)患者癌组织甲状腺转录因子(TTF)-1、p53和p63表达变化及其对术后生存的影响。方法2019年1月~2021年12月我院收治的HCC患者56例,均行肝内肿瘤切除术治疗,术后随访2年。采用免疫组化法检测癌组织和癌旁组织TTF-1、p53和p63表达。结果HCC患者癌组织TTF-1表达阳性率为33.9%,显著低于癌旁组织的73.2%(P<0.05),而p53和p63表达阳性率分别为76.8%和64.3%,显著高于癌旁组织的7.1%和32.1%(P<0.05);34例Ⅰ~Ⅱ期和19例高分化肿瘤患者癌组织TTF-1阳性率分别为47.1%和63.1%,显著高于22例Ⅲ~Ⅳ期和37例中/低分化者的13.6%和18.9%(P<0.05);Ⅲ~Ⅳ期肿瘤、38例有淋巴结转移、21例低分化肿瘤和22例有门静脉癌栓的HCC患者癌组织p53阳性率分别为95.4%、89.5%、100.0%和95.4%,显著高于Ⅰ~Ⅱ期肿瘤、无淋巴结转移、中/高分化和无门静脉癌栓者(分别为64.7%、50.0%、59.5%和64.7%,P<0.05);Ⅲ~Ⅳ期肿瘤、有淋巴结转移和低分化肿瘤组织p63阳性率分别为86.4%、73.7%和85.7%,显著高于Ⅰ~Ⅱ期、无淋巴结转移和中/高分化者的50.0%、44.4%和48.7%(P<0.05);19例TTF-1阳性者1 a和2 a生存率分别为84.2%和73.3%,显著高于37例阴性者的51.4%和35.1%(P<0.05);43例p53阳性者1 a和2 a生存率分别为53.5%和39.3%,显著低于13例阴性者的92.3%和76.9%(P<0.05);36例p63阳性者1 a和2 a生存率分别为47.2%和33.3%,显著低于20例阴性者的90.0%和75.0%(P<0.05)。结论HCC患者癌组织TTF-1阳性表达率较低,而p53和p63阳性表达率较高,而它们都可能在HCC发病过程中起作用,并影响患者生存率,值得进一步研究。 展开更多
关键词 肝细胞癌 甲状腺转录因子-1 p53基因 P63基因 生存
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Optimized thyroid transcription factor-1 core promoter-driven microRNA-7 expression effectively inhibits the growth of human non-small-cell lung cancer cells
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作者 Shipeng CHEN Lian GUAN +8 位作者 Xu ZHAO Jing YANG Longqing CHEN Mengmeng GUO Juanjuan ZHAO Chao CHEN Ya ZHOU Yong HAN Lin XU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2022年第11期915-930,共16页
Targeted gene therapy has become a promising approach for lung cancer treatment.In our previous work,we reported that the targeted expression of microRNA-7(miR-7)operated by thyroid transcription factor-1(TTF-1)promot... Targeted gene therapy has become a promising approach for lung cancer treatment.In our previous work,we reported that the targeted expression of microRNA-7(miR-7)operated by thyroid transcription factor-1(TTF-1)promoter inhibited the growth of human lung cancer cells in vitro and in vivo;however,the intervention efficiency needed to be further improved.In this study,we identified the core promoter of TTF-1(from-1299 bp to-871 bp)by 5’deletion assay and screened out the putative transcription factors nuclear factor-1(NF-1)and activator protein-1(AP-1).Further analysis revealed that the expression level of NF-1,but not AP-1,was positively connected with the activation of TTF-1 core promoter in human non-small-cell lung cancer(NSCLC)cells.Moreover,the silencing of NF-1 could reduce the expression level of miR-7 operated by TTF-1 core promoter.Of note,we optimized four distinct sequences to form additional NF-1-binding sites(TGGCA)in the sequence of TTF-1 core promoter(termed asTTF-1 promoter),and verified the binding efficiency of NF-1 on theTTF-1 promoter by electrophoretic mobility shift assay(EMSA).As expected,theTTF-1 promoter could more effectively drive miR-7 expression and inhibit the growth of human NSCLC cells in vitro,accompanied by a reduced transduction of NADH dehydrogenase(ubiquinone)1αsubcomplex 4(NDUFA4)/protein kinase B(Akt)pathway.Consistently,TTF-1 promoter-driven miR-7expression could also effectively abrogate the growth and metastasis of tumor cells in a murine xenograft model of human NSCLC.Finally,no significant changes were detected in the biological indicators or the histology of some important tissues and organs,including heart,liver,and spleen.On the whole,our study revealed that the optimized TTF-1 promoter could more effectively operate miR-7 to influence the growth of human NSCLC cells,providing a new basis for the development of microRNA-based targeting gene therapy against clinical lung cancer. 展开更多
关键词 Lung cancer thyroid transcription factor-1(TTF-1) PROMOTER MicroRNA-7(miR-7) Nuclear factor-1(NF-1)
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TTF-1启动子调控miR-7表达对人肺癌95D细胞体外凋亡的影响 被引量:3
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作者 卢佳 陈超 +6 位作者 廖珍媛 崔盼盼 雷良玉 徐华林 田丹 郑静 徐林 《西安交通大学学报(医学版)》 CAS CSCD 北大核心 2017年第2期176-181,共6页
目的观察甲状腺转录因子-1(TTF-1)启动子调控miR-7表达对人肺癌细胞体外凋亡的影响,并探讨其意义。方法将前期构建的TTF-1启动子调控miR-7真核表达载体(命名为p-T-miR-7)与对照载体p-cont分别体外瞬时转染人肺癌95D细胞,采用FCM法检测95... 目的观察甲状腺转录因子-1(TTF-1)启动子调控miR-7表达对人肺癌细胞体外凋亡的影响,并探讨其意义。方法将前期构建的TTF-1启动子调控miR-7真核表达载体(命名为p-T-miR-7)与对照载体p-cont分别体外瞬时转染人肺癌95D细胞,采用FCM法检测95D细胞凋亡比例;TUNEL法检测95D细胞凋亡情况;Western blot检测各组中磷酸化Caspase3和磷酸化Caspase9的蛋白的表达;共聚焦显微技术检测miR-7靶分子CGGBP1和EGFR蛋白的表达;最后,采用Western blot检测各组中磷酸化Akt和Erk蛋白的表达。结果体外转染48h后,与p-cont转染组相比,转染p-T-miR-7组细胞凋亡比例明显增加(P<0.05);磷酸化Caspase3和磷酸化Caspase9蛋白水平均显著增加(P<0.05),同时EGFR和CGGBP1蛋白表达均明显降低,且细胞中磷酸化Akt和Erk蛋白水平亦显著降低(P<0.05)。结论 TTF-1启动子调控miR-7表达可导致人肺癌细胞的凋亡。这为后续基于miR-7的人肺癌基因靶向治疗策略的开发提供了前期实验依据。 展开更多
关键词 miR-7 甲状腺转录因子-1(TTF-1) 启动子 基因治疗 肺癌
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胸腔积液中转移性肺腺癌细胞TTF-1的表达及基因突变分析 被引量:2
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作者 周春辉 申洪 李晓楠 《临床与实验病理学杂志》 CAS CSCD 北大核心 2007年第6期685-689,共5页
目的探讨甲状腺转录因子-1(thyroid transcription factor-1,TTF-1)在胸腔积液转移性肺腺癌细胞中的表达和基因突变情况。方法采用免疫组化SP法检测TTF-1蛋白的表达情况,采用PCR-SSCP法与DNA测序相结合对TTF-1第1、2外显子进行突变分析... 目的探讨甲状腺转录因子-1(thyroid transcription factor-1,TTF-1)在胸腔积液转移性肺腺癌细胞中的表达和基因突变情况。方法采用免疫组化SP法检测TTF-1蛋白的表达情况,采用PCR-SSCP法与DNA测序相结合对TTF-1第1、2外显子进行突变分析。结果37例转移性肺腺癌中28例表达TTF-1,11例肺外腺癌中均无表达,其检测的灵敏度为75.7%,特异度为100%。胸水中转移性腺癌肺源性组TTF-1的表达与非肺源性组比较差异具有显著性(P<0.01)。4例转移性肺腺癌TTF-1外显子2区引物2A、2S所扩增片段b出现明显电泳迁移率的改变,经DNA双向测序,均出现单碱基缺失,2例同时出现单碱基置换,突变率为10.8%。结论TTF-1在胸腔积液转移性肺腺癌的检测中具有较高的特异度和灵敏度,在排除甲状腺癌的可能性后,TTF-1可作为判断胸腔积液中转移性肺腺癌的组织特异性指标。胸水转移性肺腺癌中存在TTF-1基因外显子2区的基因突变。 展开更多
关键词 胸腔积液 肺肿瘤 甲状腺转录因子-1 免疫组织化学 PCR-SSCP 基因突变
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促甲状腺激素受体与TTF-1、Pax-8在甲状腺乳头状癌中的表达及意义 被引量:3
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作者 柳林 王丽哲 +2 位作者 张永庆 李如江 庄宝祥 《中国医院统计》 2020年第4期322-325,共4页
目的探讨促甲状腺激素受体(TSHR)、甲状腺特异性转录因子-1(TTF-1)、成框基因8编码的转录因子(Pax-8)在甲状腺乳头状癌(PTC)、桥本氏病(HT)合并甲状腺乳头状癌、结节性甲状腺肿(NG)与正常甲状腺组织中的表达及意义。方法收集2018年9月至... 目的探讨促甲状腺激素受体(TSHR)、甲状腺特异性转录因子-1(TTF-1)、成框基因8编码的转录因子(Pax-8)在甲状腺乳头状癌(PTC)、桥本氏病(HT)合并甲状腺乳头状癌、结节性甲状腺肿(NG)与正常甲状腺组织中的表达及意义。方法收集2018年9月至2019年6月在某院内分泌科确诊并在甲状腺外科行手术治疗的甲状腺组织,包括单纯PTC 16例、HT合并PTC 24例,NG 32例及癌旁正常甲状腺组织40例。将术中获得的甲状腺组织进行脱水、透明、包埋处理,分别行TSHR、TTF-1、Pax-8的免疫组织化学染色,显微镜下观察染色结果。采用χ^2检验进行多组间率的比较,差别有统计学意义时采用χ^2分割做两两比较。结果1)TSHR、TTF-1在甲状腺组织的强阳性表达在4组间的差别均有统计学意义(P<0.001,P=0.004),Pax-8在甲状腺组织的强阳性表达在4组间的差别无统计学意义(P=0.642)。2)调整检验水准α′=0.008,进一步两两比较发现:TSHR在PTC组的强阳性表达低于在NG组与癌旁正常组织(P<0.001,P=0.002),在HT合并PTC组的强阳性表达低于在NG组(P=0.001),TTF-1在HT合并PTC组的强阳性表达低于在NG组(P=0.002);其他组两两比较均无统计学意义(P>0.008)。3)在全部PTC组织中,TSHR、TTF-1的阳性表达与性别、年龄、肿瘤直径、淋巴结转移等均无关;Pax-8的阳性表达在有淋巴结转移者低于无淋巴结转移者(P=0.030),与性别、年龄、肿瘤直径无关。结论TSHR与TTF-1在PTC中的强阳性表达低于NG和癌旁正常甲状腺组织,Pax-8在不同类型甲状腺组织中表达无差别。Pax-8在PTC组织中的低表达易出现淋巴结转移。 展开更多
关键词 甲状腺乳头状癌 自身免疫性甲状腺疾病 促甲状腺激素受体 甲状腺特异性转录因子-1 成框基因8编码的转录因子
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TTF-1相关蛋白26磷酸化位点对人SP-B基因表达的影响
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作者 柴新群 邓飞涛 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2007年第6期707-710,共4页
目的探讨甲状腺转录因子-1相关蛋白26(TAP26)对人肺表面活性蛋白-B(hSP-B)基因表达的调控机制。方法采用定点诱变PCR技术,分别突变掉TAP26的4个磷酸化位点(S48,S66,T219和T167S168),并获得TAP26的4个相应突变体;通过体外细胞共转染和荧... 目的探讨甲状腺转录因子-1相关蛋白26(TAP26)对人肺表面活性蛋白-B(hSP-B)基因表达的调控机制。方法采用定点诱变PCR技术,分别突变掉TAP26的4个磷酸化位点(S48,S66,T219和T167S168),并获得TAP26的4个相应突变体;通过体外细胞共转染和荧光素酶报告基因活性值检测技术分别检测上述4个突变体对hSP-B基因启动子活性的影响。结果与野生型TAP26比较,突变体质粒TAP26(T167S168→V167A168)、TAP26(S48→A48)和TAP26(T219→V219)分别与hSP-B基因启动子共转染后所测荧光素酶活性值无明显差异(P>0.05);而当DNA浓度为400 ng的突变体质粒TAP26(S66→A66)与hSP-B基因启动子共转染后所测荧光素酶活性值则明显降低(P<0.05)。结论突变体质粒TAP26(S66→A66)不能促进hSP-B的表达,从而TAP26的S66蛋白激酶C(PKC)位点是TAP26移位到细胞核的重要部位。 展开更多
关键词 甲状腺转录因子-1相关蛋白26 肺表面活性蛋白-B 基因调节
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TTF-1基因突变与先天性甲状腺功能低下
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作者 罗燕斐 赵正言 《中国妇幼健康研究》 2006年第2期120-122,共3页
甲状腺转录因子1是含一同源盒域的转录因子,属于NKX2家族。其与甲状腺的发育有关,并且调控甲状腺球蛋白、甲状腺过氧化物酶和促甲状腺激素受体基因的转录。其基因除在甲状腺中表达,还表达于肺、前脑和垂体。甲状腺转录因子1基因突变可... 甲状腺转录因子1是含一同源盒域的转录因子,属于NKX2家族。其与甲状腺的发育有关,并且调控甲状腺球蛋白、甲状腺过氧化物酶和促甲状腺激素受体基因的转录。其基因除在甲状腺中表达,还表达于肺、前脑和垂体。甲状腺转录因子1基因突变可能导致突变的甲状腺转录因子1不能与靶基因结合。人类甲状腺转录因子1基因突变可表现为先天性甲状腺功能低下、神经系统发育异常或合并肺部症状,且均为杂合突变。 展开更多
关键词 甲状腺转录因子1 先天性甲状腺功能低下 基因突变
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Foregut duplication cysts:A report of two cases with emphasis on embryogenesis 被引量:5
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作者 Thaer Khoury Louis Rivera 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第1期130-134,共5页
Duplication cyst of the stomach with a pseudostratifie columnar ciliated epithelium is extremely rare.We de scribe two cases of these cysts,with emphasis on the immunophenotype and embryogenesis.The first patien was a... Duplication cyst of the stomach with a pseudostratifie columnar ciliated epithelium is extremely rare.We de scribe two cases of these cysts,with emphasis on the immunophenotype and embryogenesis.The first patien was a 29-year-old man who presented with crampin abdominal pain in his left lower quadrant.The secon patient was a 26-year-old woman who had a history over several years,of chronic epigastric abdominal pai radiating to her back.Both lesions were surgically re moved.They showed the same histomorphology.Th cysts were lined by a pseudostratified respiratory ep thelium with ciliated cells.The first cyst was connecte to the stomach,while the second cyst was not connect ed.Both cysts expressed thyroid transcription factor(TTF-1) and surfactant.In this report,we explore th possible embryogenesis of these lesions in the light o TTF-1 and surfactant expression. 展开更多
关键词 Duplication cyst STOMACH thyroid transcription factor-1 SURFACTANT EMBRYOgeneSIS
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TTF-1 is useful for primary site determination in duodenal metastasis 被引量:2
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作者 Tomofumi Miura Yuichi Shimaoka +7 位作者 Junichiro Nakamura Satoshi Yamada Tsutomu Miura Masahiko Yanagi Kazuhiro Sato Hiroyuki Usuda Iwao Emura Toru Takahashi 《World Journal of Gastrointestinal Oncology》 SCIE CAS 2010年第9期360-363,共4页
We report here on a case of duodenal metastasis from primary lung adenocarcinoma. A 69-year old man was diagnosed with primary lung adenocarcinoma. Four courses of combined chemotherapy with carboplatin and paclitaxel... We report here on a case of duodenal metastasis from primary lung adenocarcinoma. A 69-year old man was diagnosed with primary lung adenocarcinoma. Four courses of combined chemotherapy with carboplatin and paclitaxel associated with irradiation of 60 Gy shrunk the lung tumor. However, soon after,the para-aortic lymph node became swollen. Esophagogastroduodenoscopy revealed three duodenal tumors. Differential diagnosis between malignant lymphoma and metastatic duodenal cancer was endoscopically difficult. The histology of biopsied specimens was poorly differentiated adenocarcinoma. Immunohistochemical analysis revealed a positive reaction for thyroid transcription factor-1 (TTF-1). Thus, we concluded that these were metastatic duodenal tumors from lung adenocarcinoma. Two courses of gemcitabine led to a complete remission in this duodenal metastasisand para-aortic lymph node swelling with only scarring remaining in computed tomography. He is now on the continuous generalized chemotherapy. In conclusion, duodenal metastasis from primary lung adenocarcinoma is rare and hard to diagnose. In such an instance, TTF-1 immunostaining is crucial to obtain the correct diagnosis. 展开更多
关键词 Lung ADENOCARCINOMA DUODENAL METASTASIS thyroid transcription factor-1
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沉默Ttf1对神经元细胞及雌性大鼠弓状核内Kiss1和Gn RH基因表达的影响
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作者 臧少莲 尹小琴 +3 位作者 吕拥芬 许丽雅 郭盛 李嫔 《上海交通大学学报(医学版)》 CAS CSCD 北大核心 2020年第11期1447-1453,共7页
目的·探讨抑制神经元细胞和雌性大鼠下丘脑弓状核中甲状腺转录因子1(thyroid transcription factor 1,Ttf1)基因的表达对亲吻素(Kiss1)和促性腺激素释放激素(gonadotropin-releasing hormone,GnRH)基因表达的影响。方法·针对... 目的·探讨抑制神经元细胞和雌性大鼠下丘脑弓状核中甲状腺转录因子1(thyroid transcription factor 1,Ttf1)基因的表达对亲吻素(Kiss1)和促性腺激素释放激素(gonadotropin-releasing hormone,GnRH)基因表达的影响。方法·针对大鼠Ttf1基因的mRNA序列分别设计3对特异性的干扰序列(TTF1-shRNA1、TTF1-shRNA2、TTF1-shRNA3),包装成慢病毒质粒。以神经元细胞ND7-23为体外模型,采用real-time PCR及Western blotting技术筛选干扰效率最高的慢病毒质粒(LV-TTF1-shRNA),转染72 h后检测Kiss1和GnRH基因的表达。将LV-TTF1-shRNA和空载质粒注射到21日龄雌性大鼠的下丘脑弓状核内,分别在幼年期(25日龄)、青春发育早期(35日龄)、成年期(42日龄)检测弓状核内Kiss1和GnRH基因的表达变化并观察雌鼠的阴门开放时间。结果·慢病毒质粒转染ND7-23细胞72 h后,LV-TTF1-shRNA2组、LV-TTF1-shRNA3组的Ttf1 mRNA及蛋白表达均显著减少(均P<0.05),其中LV-TTF1-shRNA3干扰效果最佳。采用LV-TTF1-shRNA3抑制ND7-23细胞中Ttf1表达后,Kiss1和GnRH基因的mRNA表达均显著降低(均P<0.05)。雌鼠弓状核内双侧显微注射LV-TTF1-shRNA3后,25日龄、35日龄、42日龄大鼠弓状核Kiss1和GnRH的mRNA均显著降低(均P<0.05),35日龄和42日龄时KISS1蛋白水平明显降低,且雌鼠阴门开放时间延迟。结论·在细胞水平和雌性大鼠弓状核内抑制Ttf1基因表达均使得Kiss1和GnRH基因表达显著下调,也导致雌鼠阴门开放时间明显延迟。 展开更多
关键词 甲状腺转录因子1 亲吻素基因 促性腺激素释放激素 RNA干扰 慢病毒载体 青春期发育
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Involvement of chromatin and histone acetylation in the regulation of HIV-LTR by thyroid hormone receptor 被引量:4
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作者 HsiaSC WangH 《Cell Research》 SCIE CAS CSCD 2001年第1期8-16,共9页
The HIV-1 LTR controls the expression of HIV-1 viral genes and thus is critical for viral propagation and pathology. Numerous host factors have been shown to participate in the regulation of the LTR promoter. Among th... The HIV-1 LTR controls the expression of HIV-1 viral genes and thus is critical for viral propagation and pathology. Numerous host factors have been shown to participate in the regulation of the LTR promoter. Among them is the thyroid hormone (T3) receptor (TR). TR has been shown to bind to the critical region of the promoter that contain the NFbB and Sp1 binding sites. Interestingly, earlier transient transfection studies in tissue culture cells have yielded contradicting conclusions on the role of TR in LTR regulation, likely due to the use of different cell types and/or lack of proper chromatin organization. Here, using the frog oocyte as a model system that allows replication-coupled chromatin assembly, mimicking that in somatic cells, we demonstrate that unliganded heterodimers of TR and RXR (9-cis retinoic acid receptor) repress LTR while the addition of T3 relieves the repression and further activates the promoter. More importantly, we show that chromatin and unliganded TR/RXR synergize to repress the promoter in a histone deacetylase-dependent manner. 展开更多
关键词 ACETYLATION Acquired Immunodeficiency Syndrome Animals CHROMATIN DIMERIZATION gene Expression Regulation Viral HIV Long Terminal Repeat HIV-1 Histone Deacetylases HISTONES Ligands NF-kappa B OOCYTES Receptors Retinoic Acid Receptors thyroid Hormone Response Elements Retinoid X Receptors transcription Factors Xenopus laevis
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甲状腺转录因子1、细胞角蛋白7及抑癌基因p53在非小细胞肺癌中的表达及与临床病理的关系 被引量:10
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作者 王红莉 曲延刚 李虹 《医学综述》 2016年第5期1012-1014,共3页
目的探讨甲状腺转录因子1(TTF-1)、细胞角蛋白7(CK7)以及抑癌基因p53三项指标在非小细胞肺癌(NSCLC)中的表达及其与临床病理的关系。方法选取2011年2月至2012年12月恩施自治州中心医院肿瘤切除术后的NSCLC患者44例病变组织作为观察组,另... 目的探讨甲状腺转录因子1(TTF-1)、细胞角蛋白7(CK7)以及抑癌基因p53三项指标在非小细胞肺癌(NSCLC)中的表达及其与临床病理的关系。方法选取2011年2月至2012年12月恩施自治州中心医院肿瘤切除术后的NSCLC患者44例病变组织作为观察组,另选35例肺良性病变组织作为对照组。通过免疫组织化学链霉亲和素生物素过氧化物酶复合物法检测TTF-1、CK7及p53在NSCLC及肺良性组织中的表达水平,探讨NSCLC中该3项指标与临床病理特征的相关性。结果 44例NSCLC患者中的TTF-1、CK7、p53在阳性表达率分别为45.5%(20/44)、45.5%(20/44)、52.3%(23/44),明显高于对照组的2.9%(1/35)、5.7%(2/35)、2.9%(1/35)(P<0.01)。男性患者TTF-1、CK7阳性率显著低于女性(P<0.05);无吸烟史患者TTF-1、CK7阳性率显著高于有吸烟史患者(P<0.05);高分化患者TTF-1、CK7、p53阳性率显著低于中低分化患者(P<0.05)。N0患者TTF-1、CK7、p53阳性率显著低于N1+N2患者(P<0.05)。生存时间≤3年患者TTF-1、CK7、p53阳性率显著高于生存时间>3年患者(P<0.05)。结论 TTF-1、CK7以及p53在NSCLC组织中呈异常表达,与肿瘤的发生、发展相关。 展开更多
关键词 非小细胞肺癌 甲状腺转录因子1 细胞角蛋白7 抑癌基因P53 临床病理
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肺癌表皮生长因子受体突变与甲状腺转录因子-1表达的相关性探讨
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作者 姚文莲 徐薪 《武警医学》 CAS 2015年第5期455-457,共3页
目的探讨肺癌中表皮生长因子受体(epidermal growth factor receptor,EGFR)突变状态与甲状腺转录因子-1(thyroid transcription factor-1,TTF-1)表达,以及与临床病理特征之间的关系。方法收集40例手术切除肺癌标本,采用扩增阻滞预编系统... 目的探讨肺癌中表皮生长因子受体(epidermal growth factor receptor,EGFR)突变状态与甲状腺转录因子-1(thyroid transcription factor-1,TTF-1)表达,以及与临床病理特征之间的关系。方法收集40例手术切除肺癌标本,采用扩增阻滞预编系统(ARMS)法和PCR技术检测肺癌组织中EGFR基因的突变情况。分析肺癌的临床病理特征、肿瘤组织学类型与EGFR突变的关系。结果 40例中,EGFR基因突变概率为57.5%(23/40),且突变率因性别、吸烟史、肿瘤组织学类型及TTF-1表达的不同而具有统计学差异(P<0.05)。结论肺癌中EGFR基因的突变状态与TTF-1表达、组织学类型、患者性别、吸烟史有关。TTF-1强表达的女性、不吸烟的腺癌患者中EGFR具有更高的突变率。 展开更多
关键词 肺癌 表皮生长因子受体 甲状腺转录因子 病理学特征 thyroid transcription factor-1
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转录因子CREB3L1在甲状腺癌组织中的表达及生物学意义
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作者 蒋洪棉 朱良琴 冯振博 《现代医药卫生》 2023年第8期1267-1274,共8页
目的探讨转录因子环腺苷酸响应元件结合蛋白3-样1(CREB3L1)在甲状腺癌(THCA)组织中的表达及其临床意义。方法从GEO、ArrayExpress、SRA、TCGA等数据库检索下载CREB3L1 mRNA在THCA组织中的表达谱,应用Wilcoxon非参数检验检测CREB3L1 mRNA... 目的探讨转录因子环腺苷酸响应元件结合蛋白3-样1(CREB3L1)在甲状腺癌(THCA)组织中的表达及其临床意义。方法从GEO、ArrayExpress、SRA、TCGA等数据库检索下载CREB3L1 mRNA在THCA组织中的表达谱,应用Wilcoxon非参数检验检测CREB3L1 mRNA在THCA组织和正常甲状腺组织中表达水平的差异;计算CREB3L1表达值的标准化均数差(SMD)及汇总受试者工作特征(SROC)曲线下面积等指标,综合评估CREB3L1 mRNA在THCA组织中的表达水平及其对THCA的区分能力。用上述数据集批量获取CREB3L1在THCA组织中的相关过表达基因,应用CistromeDB预测CREB3L1转录靶基因,用clusterProfiler对CREB3L1在THCA组织中的相关过表达基因、CREB3L1转录靶标交集基因进行基因本体和京都基因与基因组百科全书功能注释。在THPA数据库中查看CREB3L1蛋白在THCA组织中的表达水平。结果与正常甲状腺组织比较,THCA组织中CREB3L1蛋白表达水平呈上调趋势。基于基因芯片、高通量测序数据集共纳入1175例THCA组织和791例正常甲状腺组织样本。CREB3L1 mRNA在THCA组织中呈高表达[SMD=0.11,95%可信区间(95%CI):0.01~0.20],集成SROC曲线下面积为0.62(95%CI:0.57~0.66)。CREB3L1蛋白在THCA组织中高表达患者5年生存率比低表达者低。交叉基因主要富集在细胞周期信号通路上,主要生物学功能为细胞器裂解。细胞分裂周期6蛋白(CDC6)与CREB3L1呈正相关。结论CREB3L1在THCA组织中高表达不利于患者的预后,有可能通过靶向基因CDC6参与了THCA的发生、发展。 展开更多
关键词 转录因子 甲状腺肿瘤 基因表达 生物学 cAMP响应元件结合蛋白3-样1
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Cloning of human XAF1 gene promoter and assay of its transcription activity in a variety of cell lines
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作者 Qiong CHEN Qing YU +6 位作者 Yuhu SONG Peiyuan Li Ying CHANG Zhijun WANG Lifeng LIU Wei WU Jusheng LIN 《Frontiers of Medicine》 SCIE CSCD 2009年第2期148-152,共5页
To investigate the regulation of tumor sup-pressor XAF1 gene expression in digestive system cancers,we studied XAF1 gene promoter transcription activity and mRNA level in digestive system cancer cell lines(human hepat... To investigate the regulation of tumor sup-pressor XAF1 gene expression in digestive system cancers,we studied XAF1 gene promoter transcription activity and mRNA level in digestive system cancer cell lines(human hepatoma cell line HepG2,human colon cancer cell line LoVo,and human gastric cancer cell line AGS)and nontumor cell lines(human embryonic liver cell line L02(L02 cells)and human embryonic kidney 293 cells[HEK293 cells])as controls.1395-bp-promoter fragment of XAF1 gene was amplified by polymerase chain reaction(PCR)and cloned into pGL3-basic vector and pEGFP-1 vector to assay its promoter transcription activity.The plasmids were transfected into a variety of cell lines by lipofectamine 2000.The promoter transcription activity was determined by dual-luciferase report assay,and enhanced greenfluorescent protein(EGFP)-positive cells were detected byfluorescence microscope.The expression of XAF1 mRNA in HEK293 and L02 were significantly higher than that in any of the three digestive system cancer cell lines.The dual-luciferase reporter assay showed that the promoter transcription activity in digestive system tumor cell lines transfected with pGL3-XAF1p promoter was apparently lower than that of both HEK293 and L02 cells.Expression of greenfluorescent protein(GFP)under the control of XAF1 promoter in the three digestive system cancer cell lines was lower than that of both HEK293 and L02 cells.The activities of pGL3-XAF1p in the three digestive system cancer cell lines after treatment with heat stress were significantly lower than those in the unstressed cells.The results suggested that remarkably down-regulated XAF1 mRNA expression in digestive system cancer cell lines may be due to loss of transcription activity of XAF1 promoter. 展开更多
关键词 gene X-linked inhibitor of apoptosis protein associated factor-1(XAF1) PROMOTER transcription regulation
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Correlation of TTF-1 and Napsin A expression in CT-guided percutaneous puncture tissue with the malignant biology of cancer cells
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作者 Wen-Guo Sun Yang-Liang Kong Qi Wang 《Journal of Hainan Medical University》 2018年第7期79-83,共5页
Objective: To study the correlation of thyroid transcription factor-1 (TTF-1) and Napsin A expression in CT-guided percutaneous puncture tissue with the malignant biology of cancer cells. Methods: The lung tissues tha... Objective: To study the correlation of thyroid transcription factor-1 (TTF-1) and Napsin A expression in CT-guided percutaneous puncture tissue with the malignant biology of cancer cells. Methods: The lung tissues that were obtained from CT-guided percutaneous puncture in Tongchuan People's Hospital between March 2015 and December 2017 were selected and pathologically diagnosed with lung cancer tissues (n=78) and benign tissues (n=40), the RNA was extracted, and then the expression levels of TTF-1, NapsinA, oncogenes and epithelial-mesenchymal transition (EMT) genes were determined. Results: TTF-1, Pim1, C-myc, Nr4a1, KLF4 and N-cadherin mRNA expression levels in lung cancer tissues were significantly higher than those in benign tissues whereas NapsinA, Rb, LKB1, HIPK2 and E-cadherin mRNA expression levels were significantly lower than those in benign tissues;Pim1 and C-myc mRNA expression levels in lung cancer tissues with high TTF-1 expression were significantly higher than those in lung cancer tissues with low TTF-1 expression whereas Rb and LKB1 mRNA expression levels were significantly lower than those in lung cancer tissues with low TTF-1 expression;HIPK2 and E-cadherin mRNA expression levels in lung cancer tissues with high NapsinA expression were significantly higher than those in lung cancer tissues with low NapsinA expression whereas Nr4a1, KLF4 and N-cadherin mRNA expression levels were significantly lower than those in lung cancer tissues with low NapsinA expression. Conclusion: The high expression of TTF-1 and the low expression NapsinA in CT-guided percutaneous puncture tissue can promote the cancer cell proliferation and EMT respectively. 展开更多
关键词 Lung cancer thyroid transcription factor-1 NAPSIN A Cell proliferation Epithelial-mesenchymal transition
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甲状腺转录因子-1在毒性弥漫性甲状腺肿患者甲状腺细胞中的表达及生物学意义研究
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作者 庄端蓉 胡鑫 黄惠斌 《中国现代医学杂志》 CAS 2024年第23期34-40,共7页
目的探讨甲状腺转录因子-1(NKX2-1)在毒性弥漫性甲状腺肿患者甲状腺细胞中的表达及生物学意义。方法选取2012年1月—2020年1月福建医科大学附属第二医院内分泌科和甲状腺外科接收的毒性弥漫性甲状腺肿患者为研究对象,收集300例患者的甲... 目的探讨甲状腺转录因子-1(NKX2-1)在毒性弥漫性甲状腺肿患者甲状腺细胞中的表达及生物学意义。方法选取2012年1月—2020年1月福建医科大学附属第二医院内分泌科和甲状腺外科接收的毒性弥漫性甲状腺肿患者为研究对象,收集300例患者的甲状腺组织作为观察组,另收集100例健康甲状腺组织作为对照组。比较两组的一般临床资料,以及促甲状腺激素受体抗体(TRAb)、甲状腺过氧化物酶自身抗体(TPOAb)滴度;采用实时荧光定量聚合酶链反应(qRT-PCR)检测两组NKX2-1的表达;Western blotting检测WNT通路和TGF-β通路蛋白的表达。构建NKX2-1沉默细胞株和过表达NKX2-1慢病毒的载体,比较两种情况下细胞增殖和克隆能力。结果观察组女性占比多于对照组(P<0.05),TRAb滴度和TPOAb滴度均高于对照组(P<0.05)。观察组NKX2-1基因相对表达量高于对照组(P<0.05)。NKX2-1过表达细胞株的细胞增殖率和克隆形成率均高于NKX2-1沉默细胞株(P<0.05)。NKX2-1过表达与NKX2-1沉默细胞株培养1、2、4、6、8 d的吸光度值比较,采用重复测量设计的方差分析,结果:①不同时间点的吸光度值比较,差异有统计学意义(P<0.05);②NKX2-1过表达与NKX2-1沉默细胞株的吸光度值比较,差异有统计学意义(P<0.05),NKX2-1过表达组吸光度值较高,增殖能力较好;③两组的吸光度值变化趋势比较,差异有统计学意义(P<0.05)。结论NKX2-1与毒性弥漫性甲状腺肿患者甲状腺细胞的增殖有关,对患者预后评估及治疗有重要作用。 展开更多
关键词 毒性弥漫性甲状腺肿 甲状腺转录因子-1 基因表达 细胞增殖
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Breast metastasis from lung cancer:a report of two cases and literature review 被引量:3
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作者 Li Wang Shu-Ling Wang Hong-Hong Shen Feng-Ting Niu Yun Niu 《Cancer Biology & Medicine》 CAS CSCD 2014年第3期208-215,共8页
Breast metastasis from extra-mammary malignancy is rare. An incidence of 0.4% to 1.3% has been reported in literature. The primary malignancies that most commonly metastasize to the breast are leukemia, lymphoma, and ... Breast metastasis from extra-mammary malignancy is rare. An incidence of 0.4% to 1.3% has been reported in literature. The primary malignancies that most commonly metastasize to the breast are leukemia, lymphoma, and malignant melanoma. In this report, two cases of pulmonary metastasis to the breast were presented. A 40-year-old female manifested a right breast mass of 2-month duration. After physical examination was performed, a poorly defined mass was noted in the upper outer quadrant of the right breast. Another 49-year-old female manifested right breast mass of 5-day duration. A poorly defined mass was noted in the lower inner quadrant of the right breast. Mammography results also revealed breast cancer. The patients underwent local excision. After histological and immunohistochemical analyses were conducted, a primary lung carcinoma that metastasized to the breast was diagnosed. An accurate differentiation of metastasis to the breast from primary breast cancer is very important because the treatment and prognosis of the two differ significantly. 展开更多
关键词 Lung cancer breast metastasis immunohistochemistry thyroid transcription factor-1(TTF1
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