The haemocompatibility of Ti-3Zr-2Sn-3Mo-25Nb biomedical alloy was studied after surface heparinization. A layer of sol-gel TiO2 films was applied on the alloy samples followed by active treatment in the bio-functiona...The haemocompatibility of Ti-3Zr-2Sn-3Mo-25Nb biomedical alloy was studied after surface heparinization. A layer of sol-gel TiO2 films was applied on the alloy samples followed by active treatment in the bio-functionalized solution for introducing the OH- and groups, and then the heparin was immobilized on the active TiO2 films through the electrostatic self assembly technology. It is shown that the heparinized films are mainly composed of anatase and rutile with smooth and dense surface. In vitro blood compatibility was evaluated by haemolysis test, clotting time and platelet adhesion behavior tests. The results show that the haemocompatibility of the alloy could be significantly improved by surface heparinization.展开更多
A layer of porous film containing Ca and P was prepared by the micro-arc oxidation method on the surface of a novel near β biomedical Ti-3Zr-2Sn-3Mo-25Nb alloy, and then NH2- active group was introduced to the films ...A layer of porous film containing Ca and P was prepared by the micro-arc oxidation method on the surface of a novel near β biomedical Ti-3Zr-2Sn-3Mo-25Nb alloy, and then NH2- active group was introduced to the films by activation treatment. The phase composition, surface micro-topography and elemental characteristics of the micro-arc oxidation films were investigated with XRD, SEM, EDS and XPS, and the osteoinduction of the micro-arc oxidation films was tested using the simulated body fluid immersion, the in-vitro osteoblast cultivation test and animal experiment. The results show that the oxide layer is a kind of porous ceramic intermixture and contains Ca and P. The films in the simulated body fluid can induce apatite formation, resulting in excellent bioactivity. The cell test discovers that osteoblasts can grow well on the surface of micro-arc oxidation films. And the Ti-3Zr-2Sn-3Mo-25Nb biomedical alloy coated with active porous calcium-phosphate films shows better osteoinduction in vivo.展开更多
In the present work,hierarchical nanostructured titanium dioxide(TiO2) films were fabricated on Ti-25Nb-3Mo-2Sn-3Zr(TLM) alloy for biomedical applications via one-step anodization process in ethylene glycolbased elect...In the present work,hierarchical nanostructured titanium dioxide(TiO2) films were fabricated on Ti-25Nb-3Mo-2Sn-3Zr(TLM) alloy for biomedical applications via one-step anodization process in ethylene glycolbased electrolyte containing 0.5wt% NH4F.The nanostructured TiO2 films exhibited three distinct types depending on the anodization time:top irregular nanopores(INP)/beneath regular nanopores(RNP),top INP/middle regular nanotubes(RNT)/bottom RNP and top RNT with underlying RNP.The evolution of the nanostructured TiO2 films with anodization time demonstrated that self-organizing nanopores formed at the very beginning and individual nanotubes originated from underlying nanopore dissolution.Furthermore,a modified two-stage self-organizing mechanism was introduced to illustrate the growth of the nanostructured TiO2 films.Compared with TLM titanium alloy matrix,the TiO2 films with special nano-structure hold better hydrophilicity and higher specific surface area,which lays the foundation for their biomedical applications.展开更多
基金Project (31100693/C100302) supported by the National Natural Science Foundation of ChinaProject (31011120049) supported by the Australia-China Special Fund, International Science Linkages Program co-supported by the Department of Innovation, Industry, Science and Research of Australia, and the Ministry of Science and Technology and National Science Foundation of China+1 种基金Project(2010ZDKG-96) supported by the Major Subject of "13115" Programs of Shaan’xi Province, ChinaProject (2012CB619102) supported by the National Basic Research Program of China
文摘The haemocompatibility of Ti-3Zr-2Sn-3Mo-25Nb biomedical alloy was studied after surface heparinization. A layer of sol-gel TiO2 films was applied on the alloy samples followed by active treatment in the bio-functionalized solution for introducing the OH- and groups, and then the heparin was immobilized on the active TiO2 films through the electrostatic self assembly technology. It is shown that the heparinized films are mainly composed of anatase and rutile with smooth and dense surface. In vitro blood compatibility was evaluated by haemolysis test, clotting time and platelet adhesion behavior tests. The results show that the haemocompatibility of the alloy could be significantly improved by surface heparinization.
基金Project (2005CB623904) supported by the National Basic Research Program of ChinaProject (30770586) supported by the National Natural Science Foundation of China+1 种基金Project (31011120049) supported by the Australia-China special fund, International Science Linkages Program co-supported by the Department of Innovation, Industry, Science and Research of Australia, and the Ministry of Science and Technology and National Science Foundation of ChinaProject (2010ZDKG-96) supported by the major Subject of "13115" Programs of Shaan’xi Province, China
文摘A layer of porous film containing Ca and P was prepared by the micro-arc oxidation method on the surface of a novel near β biomedical Ti-3Zr-2Sn-3Mo-25Nb alloy, and then NH2- active group was introduced to the films by activation treatment. The phase composition, surface micro-topography and elemental characteristics of the micro-arc oxidation films were investigated with XRD, SEM, EDS and XPS, and the osteoinduction of the micro-arc oxidation films was tested using the simulated body fluid immersion, the in-vitro osteoblast cultivation test and animal experiment. The results show that the oxide layer is a kind of porous ceramic intermixture and contains Ca and P. The films in the simulated body fluid can induce apatite formation, resulting in excellent bioactivity. The cell test discovers that osteoblasts can grow well on the surface of micro-arc oxidation films. And the Ti-3Zr-2Sn-3Mo-25Nb biomedical alloy coated with active porous calcium-phosphate films shows better osteoinduction in vivo.
基金National Key Technology R&D Program(2012BAI18B02)National Natural Science Foundation of China(31100693/C100302)+2 种基金Major State Basic Research Development Program of China(2012CB619102)National High Technology Research and Development Program of China(2011AA030101)Industrial Research Projects of Shaanxi Province(2012K07-03)
基金Supported by the National Natural Science Foundation of China(No.51372169)Natural Science Foundation of Tianjin(No.11JCZDJC17300)
文摘In the present work,hierarchical nanostructured titanium dioxide(TiO2) films were fabricated on Ti-25Nb-3Mo-2Sn-3Zr(TLM) alloy for biomedical applications via one-step anodization process in ethylene glycolbased electrolyte containing 0.5wt% NH4F.The nanostructured TiO2 films exhibited three distinct types depending on the anodization time:top irregular nanopores(INP)/beneath regular nanopores(RNP),top INP/middle regular nanotubes(RNT)/bottom RNP and top RNT with underlying RNP.The evolution of the nanostructured TiO2 films with anodization time demonstrated that self-organizing nanopores formed at the very beginning and individual nanotubes originated from underlying nanopore dissolution.Furthermore,a modified two-stage self-organizing mechanism was introduced to illustrate the growth of the nanostructured TiO2 films.Compared with TLM titanium alloy matrix,the TiO2 films with special nano-structure hold better hydrophilicity and higher specific surface area,which lays the foundation for their biomedical applications.
