[Objectives]This study was conducted to determine the contents of benzoylmesaconine,benzoylaconitine and benzoylhypacoitine in the decoctions of Heishun pieces,Trichosanthis Fructus and their combination.[Methods]Heis...[Objectives]This study was conducted to determine the contents of benzoylmesaconine,benzoylaconitine and benzoylhypacoitine in the decoctions of Heishun pieces,Trichosanthis Fructus and their combination.[Methods]Heishun pieces,Trichosanthis Fructus and their combination were extracted for different time periods,and then grouped.HPLC was performed using an Agilent ZORBAX SB-C 18 chromatographic column(4.6 mm×250 mm,5μm)and acetonitrile-0.02 mol/L sodium dihydrogen phosphate as the mobile phase at a flow rate of 1 mL/min and a column temperature of 30℃,and the sample volume was 20μL.The detection wavelength was 230 nm.[Results]The total amounts of benzoylmesaconine,benzoylaconitine and benzoylhypacoitine in the single decoction group of Heishun pieces were all significantly different from those in the combined decoction group at corresponding time.[Conclusions]The total content of the benzoylaconitine type increased significantly after the combined decoction of Heishun pieces and Fructus Trichosanthis,which proves the scientificity of"eighteen incompatible medicaments,19 counteraction"in traditional Chinese medicine to some extent.展开更多
[Objectives]To explore the correlation of processing technology,physical parameters and chemical components during plain stir-baking of Trichosanthis Radix.[Methods]Based on mixture uniform experiment design,the Trich...[Objectives]To explore the correlation of processing technology,physical parameters and chemical components during plain stir-baking of Trichosanthis Radix.[Methods]Based on mixture uniform experiment design,the Trichosanthis Radix was prepared by plain stir-bake method.Delphi method was used to evaluate and select the highest-scoring processed product for measuring physical parameters.UV spectrophotometry was used to determine the contents of starch and polysaccharide.The correlation and linear regression model of processing technology,physical parameters and chemical components were established with the aid of SPSS 26.0[Results]After processing by plain stir-bake method,the relative density and chromaticity showed a decreasing trend in the processed products of Trichosanthis Radix,the oxidation value,hydroscopic rate and swelling decreased firstly and then increased,and pH increased firstly and then decreased.The content of total starch decreased,the content of polysaccharide increased,and there was a negative correlation between them.There was a significant positive correlation between temperature and oxidation value,swelling and hydroscopic rate,hydroscopic rate and polysaccharide,and there was a significant negative correlation between relative density and hydroscopic rate or polysaccharide,total starch and hydroscopic rate or swelling.The linear relation model between processing technology and physical parameters and chemical components was r2>0.9.[Conclusions]After processing by plain stir-bake method,the physical parameters of Trichosanthis Radix changed,and there may be mutual conversion between total starch and polysaccharides.To a certain extent,physical parameters can be used to evaluate the quality of processed products of Trichosanthis Radix.This study is expected to provide a reference for research on quality evaluation of processed products of traditional Chinese medicine.展开更多
基金Supported by Project for Enhancing Young and Middle-aged Teacher's Scientific Research Basic Ability in Colleges and Universities of Guangxi in 2023 (2023KY0324).
文摘[Objectives]This study was conducted to determine the contents of benzoylmesaconine,benzoylaconitine and benzoylhypacoitine in the decoctions of Heishun pieces,Trichosanthis Fructus and their combination.[Methods]Heishun pieces,Trichosanthis Fructus and their combination were extracted for different time periods,and then grouped.HPLC was performed using an Agilent ZORBAX SB-C 18 chromatographic column(4.6 mm×250 mm,5μm)and acetonitrile-0.02 mol/L sodium dihydrogen phosphate as the mobile phase at a flow rate of 1 mL/min and a column temperature of 30℃,and the sample volume was 20μL.The detection wavelength was 230 nm.[Results]The total amounts of benzoylmesaconine,benzoylaconitine and benzoylhypacoitine in the single decoction group of Heishun pieces were all significantly different from those in the combined decoction group at corresponding time.[Conclusions]The total content of the benzoylaconitine type increased significantly after the combined decoction of Heishun pieces and Fructus Trichosanthis,which proves the scientificity of"eighteen incompatible medicaments,19 counteraction"in traditional Chinese medicine to some extent.
基金Science and Technology Research and Development Project of Chengde City,Hebei Province(201706A043)Young Scholar Program of Hebei Pharmaceutical Association Hospital Pharmaceutical Research Project(2020—Hbsyxhqn0029)Public Health Service Subsidy Fund Project of Chinese Medicine Department,State Administration of Traditional Chinese Medicine(Guo Zhong Yi Gui Cai Fa[2015]No.21).
文摘[Objectives]To explore the correlation of processing technology,physical parameters and chemical components during plain stir-baking of Trichosanthis Radix.[Methods]Based on mixture uniform experiment design,the Trichosanthis Radix was prepared by plain stir-bake method.Delphi method was used to evaluate and select the highest-scoring processed product for measuring physical parameters.UV spectrophotometry was used to determine the contents of starch and polysaccharide.The correlation and linear regression model of processing technology,physical parameters and chemical components were established with the aid of SPSS 26.0[Results]After processing by plain stir-bake method,the relative density and chromaticity showed a decreasing trend in the processed products of Trichosanthis Radix,the oxidation value,hydroscopic rate and swelling decreased firstly and then increased,and pH increased firstly and then decreased.The content of total starch decreased,the content of polysaccharide increased,and there was a negative correlation between them.There was a significant positive correlation between temperature and oxidation value,swelling and hydroscopic rate,hydroscopic rate and polysaccharide,and there was a significant negative correlation between relative density and hydroscopic rate or polysaccharide,total starch and hydroscopic rate or swelling.The linear relation model between processing technology and physical parameters and chemical components was r2>0.9.[Conclusions]After processing by plain stir-bake method,the physical parameters of Trichosanthis Radix changed,and there may be mutual conversion between total starch and polysaccharides.To a certain extent,physical parameters can be used to evaluate the quality of processed products of Trichosanthis Radix.This study is expected to provide a reference for research on quality evaluation of processed products of traditional Chinese medicine.
