Objective: To evaluate the short-term and middle-long-term therapeutic effects of deep puncture of Tianshu (天枢ST 25) for slow transit constipation (STC). Methods: Sixty cases of STC patients were evenly randomized i...Objective: To evaluate the short-term and middle-long-term therapeutic effects of deep puncture of Tianshu (天枢ST 25) for slow transit constipation (STC). Methods: Sixty cases of STC patients were evenly randomized into acupuncture group and medication group. In acupuncture group, electroacupuncture (EA, 20 Hz, continuous waves and tolerable strength) was applied to bilateral Tianshu (ST 25) for 30 min after deep puncture. The treatment was given once daily, 5 sessions every week, two weeks altogether. Patients of control group were ordered to take Lactulose (10 mL/time, b.i.d), two weeks altogether. Cleveland Constipation Score (CCS) and colonic transit time (CCT) were used to evaluate the therapeutic effect. Results: After treatment, both CCS and CCT showed that the therapeutic effect of acupuncture group was significantly superior to that of medication group (P<0.05). Six-months’ follow up showed that the therapeutic effect of acupuncture still maintained in 13 of the 22 cases visited, while that of control group only maintained in 1 of the 19 cases. Conclusion: The therapeutic effect of deep puncture of Tianshu (ST 25) for STC is definite and has a middle-long-term effect. The patients have no any unfavorable reactions.展开更多
Objective:To observe the effect of acupuncture on local skin tissue metabolites of Tianshu(ST25)in rats with Crohn's disease(CD)using metabolomics and to explore the mechanism of acupuncture treatment metabolites ...Objective:To observe the effect of acupuncture on local skin tissue metabolites of Tianshu(ST25)in rats with Crohn's disease(CD)using metabolomics and to explore the mechanism of acupuncture treatment metabolites and pathway on local skin tissue of Tianshu(ST25).Materials and Methods:Thirty-eight Sprague-Dawley rats were randomly assigned to a normal group(n=14)and a CD modeling group(n=24).Rats in the CD modeling group were administered with 2,4,6-trinitrobenzene sulfonic acid and ethanol enema,in addition to the normal group.Four normal rats and four CD modeling rats were selected at random after model identification was established.Furthermore,CD modeling group rats were randomly assigned to two groups of 10 rats:the model group and acupuncture group.Rats in the acupuncture group underwent acupuncture of Tianshu(ST25)and Shangjuxu(ST37)for 7 days.After the intervention,the colon tissue was collected from each group of rats and the pathological changes were observed through hematoxylin and eosin(HE)staining.The local skin tissues at Tianshu(ST25)of the rats in each group were taken,and the proton nuclear magnetic resonance technique was used to detect the metabonomics of the local skin tissues of Tianshu(ST25)in rats.Results:Compared with the normal group,the following were observed in the model group after HE staining:irregular colon morphology and other pathological changes such as intestinal mucosal hyperemia,edema,ulcers,polyps,and inflammatory cell infiltration.In the acupuncture group,colon tissue structure was relatively complete and layered,the colon gland structure was restored,and inflammatory cell infiltration was significantly improved.The choline,glycerin,glycine,guanidoacetic acid,and proline levels were significantly higher in the model group than in the normal group(P<0.05 or P<0.01).Compared with the model group,the acupuncture group had significantly increased contents of alanine,leucine,L-phenylalanine,and tyrosine in the skin(P<0.05 or P<0.01).Valine,leucine,and L-isoleucine biosynthesis and L-phenylalanine metabolism were the main metabolic pathways involved in the changes in the local skin tissues of the rats in each group,biosynthesis of tyrosine,and tryptophan.Conclusion:The change in the metabolites in the local area of Tianshu(ST25)may be related to intestinal disease.The acupuncture of Tianshu(ST25)can improve intestinal inflammatory reaction in rats with CD,and this finding may be related to the regulation of amino acid metabolites and their pathways in the local acupoint tissues of Tianshu(ST25)by acupuncture.展开更多
Herb-partitioned moxibustion can effectively mitigate visceral pain, a major symptom in inflammatory bowel disease, but the analgesic lnechanism is still unclear. Moreover, extracellular signal-regulated kinase, subst...Herb-partitioned moxibustion can effectively mitigate visceral pain, a major symptom in inflammatory bowel disease, but the analgesic lnechanism is still unclear. Moreover, extracellular signal-regulated kinase, substance P, and neurokinin-1 are involved in formation of central hyperalgesia. Thus, we postulated that the analgesic effect of herb-partitioned moxibustion may be associated with these factors. Accordingly, in this study, we established an inflammatory bowel disease visceral pain model in rat by enema with a mixed solution of 5% trinitrobenzenesulfonic acid and 50% ethanol. Bilateral Tianshu （ST25） and Qihai （CV6） points were selected for herb-partitioned moxi- bustion. Our results showed that herb-partitioned moxibustion improved visceral pain and down-regulated extracellular signal-regulated kinase, substance P, and neurokinin-1 protein and mRNA expression in dorsal root ganglia. These results indicate that down-regulation of extracellular signal-regulated kinase, substance E and neurokinin-1 protein and mRNA may be a central mechanism for the analgesic effect of herb-partitioned moxibustion.展开更多
文摘Objective: To evaluate the short-term and middle-long-term therapeutic effects of deep puncture of Tianshu (天枢ST 25) for slow transit constipation (STC). Methods: Sixty cases of STC patients were evenly randomized into acupuncture group and medication group. In acupuncture group, electroacupuncture (EA, 20 Hz, continuous waves and tolerable strength) was applied to bilateral Tianshu (ST 25) for 30 min after deep puncture. The treatment was given once daily, 5 sessions every week, two weeks altogether. Patients of control group were ordered to take Lactulose (10 mL/time, b.i.d), two weeks altogether. Cleveland Constipation Score (CCS) and colonic transit time (CCT) were used to evaluate the therapeutic effect. Results: After treatment, both CCS and CCT showed that the therapeutic effect of acupuncture group was significantly superior to that of medication group (P<0.05). Six-months’ follow up showed that the therapeutic effect of acupuncture still maintained in 13 of the 22 cases visited, while that of control group only maintained in 1 of the 19 cases. Conclusion: The therapeutic effect of deep puncture of Tianshu (ST 25) for STC is definite and has a middle-long-term effect. The patients have no any unfavorable reactions.
基金National Natural Science Foundation of China(No.81603705,No.81774438)China Postdoctoral Science Foundation Project(No.2107M612567)+4 种基金Natural Science Foundation of Hunan Province(No.2021JJ30513,No.2017JJ3245)Scientific Research Project of Hunan Provincial Department of Education(No.21A0235,No.20B444)Hunan Traditional Chinese Medicine Research Project(No.C2022027)Hunan Province Youth Backbone Teacher Training Target Project(2021)Changsha Natural Science Foundation(No.kq2208183).
文摘Objective:To observe the effect of acupuncture on local skin tissue metabolites of Tianshu(ST25)in rats with Crohn's disease(CD)using metabolomics and to explore the mechanism of acupuncture treatment metabolites and pathway on local skin tissue of Tianshu(ST25).Materials and Methods:Thirty-eight Sprague-Dawley rats were randomly assigned to a normal group(n=14)and a CD modeling group(n=24).Rats in the CD modeling group were administered with 2,4,6-trinitrobenzene sulfonic acid and ethanol enema,in addition to the normal group.Four normal rats and four CD modeling rats were selected at random after model identification was established.Furthermore,CD modeling group rats were randomly assigned to two groups of 10 rats:the model group and acupuncture group.Rats in the acupuncture group underwent acupuncture of Tianshu(ST25)and Shangjuxu(ST37)for 7 days.After the intervention,the colon tissue was collected from each group of rats and the pathological changes were observed through hematoxylin and eosin(HE)staining.The local skin tissues at Tianshu(ST25)of the rats in each group were taken,and the proton nuclear magnetic resonance technique was used to detect the metabonomics of the local skin tissues of Tianshu(ST25)in rats.Results:Compared with the normal group,the following were observed in the model group after HE staining:irregular colon morphology and other pathological changes such as intestinal mucosal hyperemia,edema,ulcers,polyps,and inflammatory cell infiltration.In the acupuncture group,colon tissue structure was relatively complete and layered,the colon gland structure was restored,and inflammatory cell infiltration was significantly improved.The choline,glycerin,glycine,guanidoacetic acid,and proline levels were significantly higher in the model group than in the normal group(P<0.05 or P<0.01).Compared with the model group,the acupuncture group had significantly increased contents of alanine,leucine,L-phenylalanine,and tyrosine in the skin(P<0.05 or P<0.01).Valine,leucine,and L-isoleucine biosynthesis and L-phenylalanine metabolism were the main metabolic pathways involved in the changes in the local skin tissues of the rats in each group,biosynthesis of tyrosine,and tryptophan.Conclusion:The change in the metabolites in the local area of Tianshu(ST25)may be related to intestinal disease.The acupuncture of Tianshu(ST25)can improve intestinal inflammatory reaction in rats with CD,and this finding may be related to the regulation of amino acid metabolites and their pathways in the local acupoint tissues of Tianshu(ST25)by acupuncture.
基金supported by the National Natural Science Foundation of China,No.81273843,81674073a grant from the National Key Basic Research Program of China(973 Program)+1 种基金No.2015CB554501the Project Fund of Shanghai Municipal Commission of Health and Family Planning of China,No.20144Y0153,2017BR047
文摘Herb-partitioned moxibustion can effectively mitigate visceral pain, a major symptom in inflammatory bowel disease, but the analgesic lnechanism is still unclear. Moreover, extracellular signal-regulated kinase, substance P, and neurokinin-1 are involved in formation of central hyperalgesia. Thus, we postulated that the analgesic effect of herb-partitioned moxibustion may be associated with these factors. Accordingly, in this study, we established an inflammatory bowel disease visceral pain model in rat by enema with a mixed solution of 5% trinitrobenzenesulfonic acid and 50% ethanol. Bilateral Tianshu （ST25） and Qihai （CV6） points were selected for herb-partitioned moxi- bustion. Our results showed that herb-partitioned moxibustion improved visceral pain and down-regulated extracellular signal-regulated kinase, substance P, and neurokinin-1 protein and mRNA expression in dorsal root ganglia. These results indicate that down-regulation of extracellular signal-regulated kinase, substance E and neurokinin-1 protein and mRNA may be a central mechanism for the analgesic effect of herb-partitioned moxibustion.
