Objective To report outcomes of nonsurgical and surgical management of Menire＇s disease at Beijing Tiantan Hospital. Methods Patients with Menire＇s disease were categorized into groups based on hearing and quality o...Objective To report outcomes of nonsurgical and surgical management of Menire＇s disease at Beijing Tiantan Hospital. Methods Patients with Menire＇s disease were categorized into groups based on hearing and quality of life. Individualized management was provided, including life style modification, drug therapies, endolymphatic sac decompression and labyrin- thectomy. Treatment outcomes were evaluated during up to 24 months follow up. Results Eighty seven patients underwent life style modification and drug therapies. The vertigo control rate of Grade A and B was 76.9% and 83.8% respectively. Six patients received surgical management, including endolymphatic sac decompression （n = 5） and labyrinthecto- my （n = 1）. For these patients, the vertigo control rate of Grade A and B was 80% and 100%, respectively. Conclu- lsions Management of Meni6re＇s disease depends on several factors, i.e. severities of vertigo and hearing loss, quality of life, surgical contraindications and patient subjective desire. The treatment is drug therapies for the majority of patients, as well as life style modification. Surgical indications are rare and the least invasive procedures should be considered first. The results of surgery are generally satisfying.展开更多
The vaccinia virus Tiantan(VTT)is widely utilized as a smallpox vaccine in China and holds significant impor-tance in the prevention of diseases stemming from poxvirus infections.Nevertheless,few studies have investi-...The vaccinia virus Tiantan(VTT)is widely utilized as a smallpox vaccine in China and holds significant impor-tance in the prevention of diseases stemming from poxvirus infections.Nevertheless,few studies have investi-gated the influence of VTT infection on host gene expression.In this study,we constructed time series transcriptomic profiles of HeLa cells infected with both VTT and western reserve(WR)strains.We observed similar patterns of viral gene expression,while the expression levels of host genes varied between the two strains.There was an immediate and significant repression of host gene expression,particularly in genes asso-ciated with oxidative phosphorylation.Conversely,genes involved in nerve growth factor(NGF)-stimulated transcription were significantly activated.The upregulation of genes linked to the ribonucleic acid(RNA)-induced silencing complex(RISC)suggested a potential role for posttranscriptional regulation in the interac-tion between the vaccinia virus and the host.In the later stages of infection,pathways such as extracellular matrix organization,neutrophil degranulation,complement and interferon responses,translation,and pro-grammed cell death are largely inhibited.A significant number of host genes exhibit correlations with changes in the expression levels of viral genes.The host genes that are negatively correlated with viral genes are mainly enriched in pathways associated with translation and the response to viral infection.This study significantly contributes to advancing our understanding of the dynamics between the vaccinia virus and the host,improv-ing the application of VTTs and facilitating the development of effective vaccines against diseases such as smallpox and monkeypox.展开更多
文摘Objective To report outcomes of nonsurgical and surgical management of Menire＇s disease at Beijing Tiantan Hospital. Methods Patients with Menire＇s disease were categorized into groups based on hearing and quality of life. Individualized management was provided, including life style modification, drug therapies, endolymphatic sac decompression and labyrin- thectomy. Treatment outcomes were evaluated during up to 24 months follow up. Results Eighty seven patients underwent life style modification and drug therapies. The vertigo control rate of Grade A and B was 76.9% and 83.8% respectively. Six patients received surgical management, including endolymphatic sac decompression （n = 5） and labyrinthecto- my （n = 1）. For these patients, the vertigo control rate of Grade A and B was 80% and 100%, respectively. Conclu- lsions Management of Meni6re＇s disease depends on several factors, i.e. severities of vertigo and hearing loss, quality of life, surgical contraindications and patient subjective desire. The treatment is drug therapies for the majority of patients, as well as life style modification. Surgical indications are rare and the least invasive procedures should be considered first. The results of surgery are generally satisfying.
基金supported by the National Key Research and Development Program of China(2022YFC2303401)the National Natural Science Foundation of China(82241066).
文摘The vaccinia virus Tiantan(VTT)is widely utilized as a smallpox vaccine in China and holds significant impor-tance in the prevention of diseases stemming from poxvirus infections.Nevertheless,few studies have investi-gated the influence of VTT infection on host gene expression.In this study,we constructed time series transcriptomic profiles of HeLa cells infected with both VTT and western reserve(WR)strains.We observed similar patterns of viral gene expression,while the expression levels of host genes varied between the two strains.There was an immediate and significant repression of host gene expression,particularly in genes asso-ciated with oxidative phosphorylation.Conversely,genes involved in nerve growth factor(NGF)-stimulated transcription were significantly activated.The upregulation of genes linked to the ribonucleic acid(RNA)-induced silencing complex(RISC)suggested a potential role for posttranscriptional regulation in the interac-tion between the vaccinia virus and the host.In the later stages of infection,pathways such as extracellular matrix organization,neutrophil degranulation,complement and interferon responses,translation,and pro-grammed cell death are largely inhibited.A significant number of host genes exhibit correlations with changes in the expression levels of viral genes.The host genes that are negatively correlated with viral genes are mainly enriched in pathways associated with translation and the response to viral infection.This study significantly contributes to advancing our understanding of the dynamics between the vaccinia virus and the host,improv-ing the application of VTTs and facilitating the development of effective vaccines against diseases such as smallpox and monkeypox.
