With the interdisciplinary development of multiple disciplines,mathematical models play a role as a bridge in the research process of Chinese herbal compounds,which can make full use of the interrelationships of knowl...With the interdisciplinary development of multiple disciplines,mathematical models play a role as a bridge in the research process of Chinese herbal compounds,which can make full use of the interrelationships of knowledge,making the research and use of Chinese herbal compounds from complex and cumbersome to simple.This paper reviewed the research and application of mathematical models in the production and preparation,pharmacodynamics,pharmacokinetics and other aspects of Chinese herbal compound prescriptions,providing a theoretical basis for the development of the modern Chinese medicine industry.展开更多
目的通过超高效液相色谱—质谱联用(ultra-performance liquid chromatography-orbitrap-mass spectrometry/mass spectrometry,UPLC-Orbitrap-MS/MS)对通络益晴方的化学成分进行快速分析及初步鉴定。方法使用ZORBAX Eclipse Plus C 18...目的通过超高效液相色谱—质谱联用(ultra-performance liquid chromatography-orbitrap-mass spectrometry/mass spectrometry,UPLC-Orbitrap-MS/MS)对通络益晴方的化学成分进行快速分析及初步鉴定。方法使用ZORBAX Eclipse Plus C 18色谱柱,以乙腈-0.1%甲酸水梯度洗脱,流速0.4 mL/min,柱温35℃;采用电喷雾离子源,正、负离子模式扫描,通过保留时间、精确相对分子质量和二级质谱裂解碎片,结合参考文献,对所得成分进行分析与初步鉴定。结果根据建立的色谱条件,从通络益晴方中初步鉴定出94个化学成分,其中有黄酮类化合物31个、酚酸类化合物14个、脂肪酸类化合物9个、皂苷类化合物6个、异黄酮类化合物5个、氨基酸类化合物4个、三萜类化合物4个、醛类化合物4个、多肽类化合物3个、苯丙素类化合物3个、醇类化合物3个,以及其他类化合物8个;正、负离子扫描模式下得到8个重复的成分。结论UPLC-Orbitrap-MS/MS技术能快速、初步鉴定出通络益晴方中化学成分,可为通络益晴方物质基础研究和新药研发提供依据。展开更多
Objective: To investigate the effects of Pinggan Prescription(平肝方, PGP) on hypertension by the associated methods of metabonomic and pharmacodynamic. Methods: A total of 32 male spontaneously hypertensive rats(SHRs...Objective: To investigate the effects of Pinggan Prescription(平肝方, PGP) on hypertension by the associated methods of metabonomic and pharmacodynamic. Methods: A total of 32 male spontaneously hypertensive rats(SHRs) were randomly divided into two groups by using the random number table method:a treatment group(n=18) and a model group(n=14). The Wistar rats(n=14) were used as the normal group.Different prescription were used to intervene three groups: the treatment group in which PGP extract was administered orally at a dose of 18.336 g/kg(PGP/body weight), and the model group in which physiological saline was administered at the equivalent dose. The same treatment was applied to the normal group as the model group. The blood pressure was measured by tail-cuff method, and pharmacodynamic indexes including cyclic adenosine monophosphate(c AMP) and angiotensinⅡ(Ang Ⅱ) were tested by enzyme-linked immunosorbent assay. The plasma samples from three groups were detected by gas chromatography-mass spectrometry(GC-MS). Results: Compared with the model group, blood pressure of treatment group was obviously reduced after continuous curing with PGP(P<0.01). The pharmacodynamic results illustrated that the content of Ang Ⅱincreased with the raised blood pressure and the c AMP expressed the converse trend. After curing with PGP, the content of Ang Ⅱ decreased, the difference between model group and treatment group was signi?cant(P<0.01),and the c AMP expressed the converse trend. Five potential biomarkers were identi?ed, including arachidonic acid,hexadecanoic acid, elaidic acid, octadecanedioic acid and 9,12-octadecadienoic acid. These metabolites had shown signi?cantly changes as followed: arachidonic acid, hexadecanoic acid and elaidic acid were signi?cantly higher and octadecanedioic acid and 9,12-octadecadienoic acid were lowered in the model group than those in the normal group. After the treatment of PGP, the metabolites had the trends of returning to normal along with the reduced blood pressure. Conclusions: PGP intervention for hypertension played a major role in the metabolism of arachidonic acid and linoleic acid. Metabonomic with pharmacodynamic methods could be potentially powerful tools to investigate the mechanism of Chinese medicine.展开更多
文摘With the interdisciplinary development of multiple disciplines,mathematical models play a role as a bridge in the research process of Chinese herbal compounds,which can make full use of the interrelationships of knowledge,making the research and use of Chinese herbal compounds from complex and cumbersome to simple.This paper reviewed the research and application of mathematical models in the production and preparation,pharmacodynamics,pharmacokinetics and other aspects of Chinese herbal compound prescriptions,providing a theoretical basis for the development of the modern Chinese medicine industry.
文摘目的通过超高效液相色谱—质谱联用(ultra-performance liquid chromatography-orbitrap-mass spectrometry/mass spectrometry,UPLC-Orbitrap-MS/MS)对通络益晴方的化学成分进行快速分析及初步鉴定。方法使用ZORBAX Eclipse Plus C 18色谱柱,以乙腈-0.1%甲酸水梯度洗脱,流速0.4 mL/min,柱温35℃;采用电喷雾离子源,正、负离子模式扫描,通过保留时间、精确相对分子质量和二级质谱裂解碎片,结合参考文献,对所得成分进行分析与初步鉴定。结果根据建立的色谱条件,从通络益晴方中初步鉴定出94个化学成分,其中有黄酮类化合物31个、酚酸类化合物14个、脂肪酸类化合物9个、皂苷类化合物6个、异黄酮类化合物5个、氨基酸类化合物4个、三萜类化合物4个、醛类化合物4个、多肽类化合物3个、苯丙素类化合物3个、醇类化合物3个,以及其他类化合物8个;正、负离子扫描模式下得到8个重复的成分。结论UPLC-Orbitrap-MS/MS技术能快速、初步鉴定出通络益晴方中化学成分,可为通络益晴方物质基础研究和新药研发提供依据。
基金Supported by the National Natural Science Foundation of China(No.81273700 and No.81102549)
文摘Objective: To investigate the effects of Pinggan Prescription(平肝方, PGP) on hypertension by the associated methods of metabonomic and pharmacodynamic. Methods: A total of 32 male spontaneously hypertensive rats(SHRs) were randomly divided into two groups by using the random number table method:a treatment group(n=18) and a model group(n=14). The Wistar rats(n=14) were used as the normal group.Different prescription were used to intervene three groups: the treatment group in which PGP extract was administered orally at a dose of 18.336 g/kg(PGP/body weight), and the model group in which physiological saline was administered at the equivalent dose. The same treatment was applied to the normal group as the model group. The blood pressure was measured by tail-cuff method, and pharmacodynamic indexes including cyclic adenosine monophosphate(c AMP) and angiotensinⅡ(Ang Ⅱ) were tested by enzyme-linked immunosorbent assay. The plasma samples from three groups were detected by gas chromatography-mass spectrometry(GC-MS). Results: Compared with the model group, blood pressure of treatment group was obviously reduced after continuous curing with PGP(P<0.01). The pharmacodynamic results illustrated that the content of Ang Ⅱincreased with the raised blood pressure and the c AMP expressed the converse trend. After curing with PGP, the content of Ang Ⅱ decreased, the difference between model group and treatment group was signi?cant(P<0.01),and the c AMP expressed the converse trend. Five potential biomarkers were identi?ed, including arachidonic acid,hexadecanoic acid, elaidic acid, octadecanedioic acid and 9,12-octadecadienoic acid. These metabolites had shown signi?cantly changes as followed: arachidonic acid, hexadecanoic acid and elaidic acid were signi?cantly higher and octadecanedioic acid and 9,12-octadecadienoic acid were lowered in the model group than those in the normal group. After the treatment of PGP, the metabolites had the trends of returning to normal along with the reduced blood pressure. Conclusions: PGP intervention for hypertension played a major role in the metabolism of arachidonic acid and linoleic acid. Metabonomic with pharmacodynamic methods could be potentially powerful tools to investigate the mechanism of Chinese medicine.
