[ Objective] To analyze the genetic structure and genetic differentiation of Tibetan Mastiff populations and provide a theoretical basis for the protection and rational development and utilization of the Tibetan Masti...[ Objective] To analyze the genetic structure and genetic differentiation of Tibetan Mastiff populations and provide a theoretical basis for the protection and rational development and utilization of the Tibetan Mastiff breed resources. [ Method] A total of 86 blood samples were collected from two populations of Hequ Tibetan Mastiff （HTM） and Qinghai Tibetan Mastiff （QTM）. The genetic structure and genetic differentiation of nine blood protein loci of ＂If, Po, Es-1, Es-2, S（alpha）2, Hb, AIb, Pr and Amy between these two populations were examined by vertical polyacrylam- ide gel electrophoresis with discontinuous buffer system. [ Result] The effective number of alleles （Ne）, Shannon genetic diversity index （I） and Nei average expected gene heterozygosity （H） of the HTM were respectively 1.690 7, 0.485 5 and 0.295 9, and these were higher than those of QTM. The Nei＇s standard genetic distance （D） between HTM and QTM was 0.012 3, and the degree of genetic similarity was 0.987 8. The gene flow （Nm） of these two Tibetan Mastiff populations was 14.543 7. [ Conclusion] The genetic variability of intra-population of HTM was higher than that of QTM, and the genetic differentiation of inter-population was very low （ GsT = 0.018 7）. Thereby, the great gene flow （ Nm = 14.543 7） is the main factor leading to the low genetic differentiation of the inter-population in different geographical groups.展开更多
Many studies have been reported the origin and evolution of domesticated animals, particularly dogs, however, few have system-atically examined the Tibetan Mastiff (TM), a dog that lives primarily in the Qing-Tibet Pl...Many studies have been reported the origin and evolution of domesticated animals, particularly dogs, however, few have system-atically examined the Tibetan Mastiff (TM), a dog that lives primarily in the Qing-Tibet Plateau. Here, we study the origin and evolution of TM based on the population comparison of mtDNA D-loop sequences from TM and other dogs across the world. Intriguingly, non-simultaneous arrivals of Tibetan Mastiffs ancestors to Tibet occurred, which may result from continuous Tibetans' settlement on Qing-Tibet Plateau supported by archaeological and genetic evidence. High genetic and haplotype diversity and robust haplotypes sharing with other dogs unique to East Asia are observed in Tibetan Mastiff, supporting that it is a very archaic breed derived probably from East Asia.展开更多
Genetic diversity and differentiation of four natural populations of \%Quercus mongolica \%in Northeast China were studied with the method of RAPD.Through the amplification with 48 random primers,344 repeatable loci w...Genetic diversity and differentiation of four natural populations of \%Quercus mongolica \%in Northeast China were studied with the method of RAPD.Through the amplification with 48 random primers,344 repeatable loci were detected and 245 loci were polymorphic.Percentage of polymorphic loci was 71.22%.According to the measurement of Shannon index,genetic diversity of \%Quercus mongolica\% was on relative high level and genetic variation mainly distributed within population (64.50%).In the studied populations of \%Quercus mongolica,\%the level of genetic diversity of OLJ was the highest,that of OHH was the lowest, and that of OSZ and OHL were the middle.The average genetic distance within populations was 0.0962 ,that among populations was 0.1290.Abundant genetic diversity of Quercus mongolica assured the steady existence and development of its populations.展开更多
基金supported by the Project of Gansu Province Scientific Department(GKC-97-27-5)Young Teacher Project of Tianshui Normal University
文摘[ Objective] To analyze the genetic structure and genetic differentiation of Tibetan Mastiff populations and provide a theoretical basis for the protection and rational development and utilization of the Tibetan Mastiff breed resources. [ Method] A total of 86 blood samples were collected from two populations of Hequ Tibetan Mastiff （HTM） and Qinghai Tibetan Mastiff （QTM）. The genetic structure and genetic differentiation of nine blood protein loci of ＂If, Po, Es-1, Es-2, S（alpha）2, Hb, AIb, Pr and Amy between these two populations were examined by vertical polyacrylam- ide gel electrophoresis with discontinuous buffer system. [ Result] The effective number of alleles （Ne）, Shannon genetic diversity index （I） and Nei average expected gene heterozygosity （H） of the HTM were respectively 1.690 7, 0.485 5 and 0.295 9, and these were higher than those of QTM. The Nei＇s standard genetic distance （D） between HTM and QTM was 0.012 3, and the degree of genetic similarity was 0.987 8. The gene flow （Nm） of these two Tibetan Mastiff populations was 14.543 7. [ Conclusion] The genetic variability of intra-population of HTM was higher than that of QTM, and the genetic differentiation of inter-population was very low （ GsT = 0.018 7）. Thereby, the great gene flow （ Nm = 14.543 7） is the main factor leading to the low genetic differentiation of the inter-population in different geographical groups.
基金supported by the National Basic Research Program of China (2007CB825702)
文摘Many studies have been reported the origin and evolution of domesticated animals, particularly dogs, however, few have system-atically examined the Tibetan Mastiff (TM), a dog that lives primarily in the Qing-Tibet Plateau. Here, we study the origin and evolution of TM based on the population comparison of mtDNA D-loop sequences from TM and other dogs across the world. Intriguingly, non-simultaneous arrivals of Tibetan Mastiffs ancestors to Tibet occurred, which may result from continuous Tibetans' settlement on Qing-Tibet Plateau supported by archaeological and genetic evidence. High genetic and haplotype diversity and robust haplotypes sharing with other dogs unique to East Asia are observed in Tibetan Mastiff, supporting that it is a very archaic breed derived probably from East Asia.
文摘Genetic diversity and differentiation of four natural populations of \%Quercus mongolica \%in Northeast China were studied with the method of RAPD.Through the amplification with 48 random primers,344 repeatable loci were detected and 245 loci were polymorphic.Percentage of polymorphic loci was 71.22%.According to the measurement of Shannon index,genetic diversity of \%Quercus mongolica\% was on relative high level and genetic variation mainly distributed within population (64.50%).In the studied populations of \%Quercus mongolica,\%the level of genetic diversity of OLJ was the highest,that of OHH was the lowest, and that of OSZ and OHL were the middle.The average genetic distance within populations was 0.0962 ,that among populations was 0.1290.Abundant genetic diversity of Quercus mongolica assured the steady existence and development of its populations.
