This study was conducted to investigate the effects of extracts of 4 Tibetan veterinary drugs on bacterial diarrhea, and to guide the clinical treatment of the disease. The inhibitory effects of the extracts of the 4 ...This study was conducted to investigate the effects of extracts of 4 Tibetan veterinary drugs on bacterial diarrhea, and to guide the clinical treatment of the disease. The inhibitory effects of the extracts of the 4 Tibetan veterinary drugs, Veronica ciliate Fisch, Usnea diffracta Vain, Sophoraflavescens var. flavescens, Lamiophlomis rotata （ Benth. ） Kudo on 14 common diarrheagenic bacteria were detected by K-B diffusion method and micro-broth dilution method. Optimization was performed on prescriptions of the 4 Tibetan veterinary drugs using orthogonal design software, and a mouse bacterial diarrhea model was established with a clinical isolate, Salmonella blegdam. According to the LD50 value of the optimal prescription of the 4 Tibetan veterinary drugs, the established mouse bacterial diarrhea model was treated in 3 dose groups, i. e. , the high, middle and low dose groups （0. 060, 0. 030 and 0. 020 g/ml, respectively）. The results showed that the 4 Tibetan veterinary drugs had very good inhibitory effects on most of the tested diarrheagenic bacteria, and among them, U. diffracta had better inhibitory effects on all the tested bacteria. The optimal prescription of the d Tibetan veterinary drugs （high dose, 0.06 g/ml） exhibited very good inhibitory effect on mouse diarrhea, indicating that the prescription has very good anti-diarrhea effect, which is beneficial to the clinical treatment of such disease and the development of Tibetan veterinary drugs.展开更多
Background:The Tibetan medicine Sug-Mel-sum-thang(SMST)is often used to treat insomnia disorder(ID);however,the underlying mechanism remains unclear.In this study,network pharmacology prediction was conducted to analy...Background:The Tibetan medicine Sug-Mel-sum-thang(SMST)is often used to treat insomnia disorder(ID);however,the underlying mechanism remains unclear.In this study,network pharmacology prediction was conducted to analyze the molecular mechanisms of SMST in treating ID.Methods:The active ingredients of the three herbs used in SMST were obtained from the Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences.Chemistry Database[DB/OL].The chemical formulae of the active ingredients were downloaded from PubChem in canonical SMILES format.These were then imported into the SwissTargetPrediction platform to identify drug targets.ID targets were obtained from databases such as DrugBank,GeneCards,and DisGeNET.Potential targets of SMST for the treatment of ID were identified using the online Venny mapping platform.The potential target protein-protein interaction was constructed using the STRING platform,and the obtained protein-protein interaction information was imported into Cytoscape 3.7.1 for image optimization and core gene extraction and ClueGO in Cytoscape 3.7.1 was used for the enrichment analysis of potential targets.Cytoscape 3.7.1 was also used to construct the regulatory network of the Tibetan medicine compound target pathway and conduct a topological analysis.Results:The mechanism of action of SMST in the treatment of ID involved 72 compounds,including nerolidol,apigenin,luteolin,and piperine.The key targets identified were COMT,CNR1,AKT1,SLC6A4,TNF,CTNNB1,and CHRNA4.The enrichment analysis obtained 69 KEGG pathways,mainly related to Alzheimer’s disease,cAMP signaling pathway,serotonergic synapse,and other pathways.The core active ingredient molecule docked with the target to a higher degree.The results showed that the active ingredient exhibited good binding activity with the related targets.Some parts with good docking fractions were selected for surface plasmon resonance analysis.Conclusion:Various active components in SMST play a role in the treatment of ID by acting on key targets,such as COMT and CNR1,to regulate multiple signaling pathways.展开更多
By consulting ancient Tibetan medical literature such as books of materia medica,medical works,and prescription books,as well as modern and contemporary literature and materials,and in combination with the interview a...By consulting ancient Tibetan medical literature such as books of materia medica,medical works,and prescription books,as well as modern and contemporary literature and materials,and in combination with the interview and investigation of clinical use and expert consultation,we collated and verified the name,variety classification,plant origin,application and other recorded information of Tibetan herb"Azhong",and summarized and analyzed the phenomenon of mixed use or substitution of medicinal plants.According to textual research,it first appeared in the Four Medical Tantras.The Tibetan herb"Azhong"is simply recorded in the ancient books and Materia Medica,which are collectively called"Azhong".Since the 15 th century,there have been varieties such as Za Azhong,Kan Azhong,Xing Azhong,Pumou Azhong,Zha Azhong,Bang Azhong,and the top grade is called Azhong Nabu and Nacang Azhong,and the bottom grade is Azhong Gabu.According to modern literature,the origin of"Azhong"involves 12 species of plants belonging to 8 genera,5 families.At present,Arenaria kansuensis Maxim,Ajania purpurea Shih.and Thalictrum squamiferum Lecoy are the mainstream medicinal materials of"Azhong".There is a certain basis for clinical application and theoretical records in the literature in terms of the mutual alternative use of Tibetan medicinal materials such as"Azhong"and traditional medicinal materials in different regions.Therefore,it is necessary to use modern chemical technology and pharmacological experiments to conduct a comparative study on the material basis and biological activity of different varieties of medicinal materials,and carry out a survey on resources and use status,so as to standardize the use of their varieties and origins and improve the safety of clinical medication.展开更多
基金Supported by the Science and Technology Training Plan Project of Sichuan Province(2016KZ0007)the Fundamental Research Funds for the Central Universitiesof Southwest University for Nationalities(2017NZYQN38)
文摘This study was conducted to investigate the effects of extracts of 4 Tibetan veterinary drugs on bacterial diarrhea, and to guide the clinical treatment of the disease. The inhibitory effects of the extracts of the 4 Tibetan veterinary drugs, Veronica ciliate Fisch, Usnea diffracta Vain, Sophoraflavescens var. flavescens, Lamiophlomis rotata （ Benth. ） Kudo on 14 common diarrheagenic bacteria were detected by K-B diffusion method and micro-broth dilution method. Optimization was performed on prescriptions of the 4 Tibetan veterinary drugs using orthogonal design software, and a mouse bacterial diarrhea model was established with a clinical isolate, Salmonella blegdam. According to the LD50 value of the optimal prescription of the 4 Tibetan veterinary drugs, the established mouse bacterial diarrhea model was treated in 3 dose groups, i. e. , the high, middle and low dose groups （0. 060, 0. 030 and 0. 020 g/ml, respectively）. The results showed that the 4 Tibetan veterinary drugs had very good inhibitory effects on most of the tested diarrheagenic bacteria, and among them, U. diffracta had better inhibitory effects on all the tested bacteria. The optimal prescription of the d Tibetan veterinary drugs （high dose, 0.06 g/ml） exhibited very good inhibitory effect on mouse diarrhea, indicating that the prescription has very good anti-diarrhea effect, which is beneficial to the clinical treatment of such disease and the development of Tibetan veterinary drugs.
基金supported by grants from the Projects of the National Social Science Foundation of China(20XMZ026)the Qinghai Provincial Natural Science Foundation Applied Basic Research Project(2019-ZJ-7088).
文摘Background:The Tibetan medicine Sug-Mel-sum-thang(SMST)is often used to treat insomnia disorder(ID);however,the underlying mechanism remains unclear.In this study,network pharmacology prediction was conducted to analyze the molecular mechanisms of SMST in treating ID.Methods:The active ingredients of the three herbs used in SMST were obtained from the Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences.Chemistry Database[DB/OL].The chemical formulae of the active ingredients were downloaded from PubChem in canonical SMILES format.These were then imported into the SwissTargetPrediction platform to identify drug targets.ID targets were obtained from databases such as DrugBank,GeneCards,and DisGeNET.Potential targets of SMST for the treatment of ID were identified using the online Venny mapping platform.The potential target protein-protein interaction was constructed using the STRING platform,and the obtained protein-protein interaction information was imported into Cytoscape 3.7.1 for image optimization and core gene extraction and ClueGO in Cytoscape 3.7.1 was used for the enrichment analysis of potential targets.Cytoscape 3.7.1 was also used to construct the regulatory network of the Tibetan medicine compound target pathway and conduct a topological analysis.Results:The mechanism of action of SMST in the treatment of ID involved 72 compounds,including nerolidol,apigenin,luteolin,and piperine.The key targets identified were COMT,CNR1,AKT1,SLC6A4,TNF,CTNNB1,and CHRNA4.The enrichment analysis obtained 69 KEGG pathways,mainly related to Alzheimer’s disease,cAMP signaling pathway,serotonergic synapse,and other pathways.The core active ingredient molecule docked with the target to a higher degree.The results showed that the active ingredient exhibited good binding activity with the related targets.Some parts with good docking fractions were selected for surface plasmon resonance analysis.Conclusion:Various active components in SMST play a role in the treatment of ID by acting on key targets,such as COMT and CNR1,to regulate multiple signaling pathways.
基金Supported by School-level Research and Innovation Team Cultivation Project of University of Tibetan Medicine in 2023Doctoral Program Construction Graduate Innovation Project in 2023 (BSDJS-YS-2023036).
文摘By consulting ancient Tibetan medical literature such as books of materia medica,medical works,and prescription books,as well as modern and contemporary literature and materials,and in combination with the interview and investigation of clinical use and expert consultation,we collated and verified the name,variety classification,plant origin,application and other recorded information of Tibetan herb"Azhong",and summarized and analyzed the phenomenon of mixed use or substitution of medicinal plants.According to textual research,it first appeared in the Four Medical Tantras.The Tibetan herb"Azhong"is simply recorded in the ancient books and Materia Medica,which are collectively called"Azhong".Since the 15 th century,there have been varieties such as Za Azhong,Kan Azhong,Xing Azhong,Pumou Azhong,Zha Azhong,Bang Azhong,and the top grade is called Azhong Nabu and Nacang Azhong,and the bottom grade is Azhong Gabu.According to modern literature,the origin of"Azhong"involves 12 species of plants belonging to 8 genera,5 families.At present,Arenaria kansuensis Maxim,Ajania purpurea Shih.and Thalictrum squamiferum Lecoy are the mainstream medicinal materials of"Azhong".There is a certain basis for clinical application and theoretical records in the literature in terms of the mutual alternative use of Tibetan medicinal materials such as"Azhong"and traditional medicinal materials in different regions.Therefore,it is necessary to use modern chemical technology and pharmacological experiments to conduct a comparative study on the material basis and biological activity of different varieties of medicinal materials,and carry out a survey on resources and use status,so as to standardize the use of their varieties and origins and improve the safety of clinical medication.
