The Qaidam Basin in the NE Tibetan Plateau has contributed the largest amount of potash in China.However,how the potash was formed has long been a subject of debate.Here we carried out a deep drilling
为获得藏灵菇马克斯克鲁维酵母M3菌株胆盐水解酶(BSH)的纯品,探讨了BSH粗品的分离纯化方法。粗酶液采用硫酸铵分级沉淀,再以DEAE-Sepharose Fast Flow离子交换介质,分别考察缓冲液pH值、流速和洗脱方式等对BSH分离纯化的影响。结果表明,...为获得藏灵菇马克斯克鲁维酵母M3菌株胆盐水解酶(BSH)的纯品,探讨了BSH粗品的分离纯化方法。粗酶液采用硫酸铵分级沉淀,再以DEAE-Sepharose Fast Flow离子交换介质,分别考察缓冲液pH值、流速和洗脱方式等对BSH分离纯化的影响。结果表明,在40%～70%饱和度的硫酸铵条件下BSH提取效率最高。最佳层析条件为采用pH值为6.5、50mmol/L磷酸钠缓冲体系、1.5mL/min的流速,进行分步洗脱(100mmol/L、350mmol/L、500mmol/L～600mmol/L NaCl及50mmol/L磷酸盐缓冲液3步洗脱)。在优化纯化条件下BSH的比活力可达479.55AU/mg,是原粗酶液的23.66倍。展开更多
After the bursting of Huiten Nor in Hoh Xil Region in September, 2011, the topic on whether the water overflowed from the Salt Lake would enter into the Chumaer River and become the northernmost source of the Yangtze ...After the bursting of Huiten Nor in Hoh Xil Region in September, 2011, the topic on whether the water overflowed from the Salt Lake would enter into the Chumaer River and become the northernmost source of the Yangtze River has aroused wide concern from public and academic field. Based on Landsat TM/ETM+/OLI remote sensing images during 2010–2015, SRTM 1 arc-second data, Google Earth elevation data and the observation data from the Wudaoliang meteorological station, the study initially analyzed the variations of the Salt Lake and its overflowing condition and probability. The results showed that the area of the Salt Lake expanded sharply from October 2011 to April 2013, and then it stepped into a stable expansion period. On October 27, 2015, the area of the Salt Lake had arrived at 151.38 km^2, which was about 3.35 times the area of the lake on March 3, 2010. The Salt Lake will overflow when its area reaches the range from 218.90 km^2 to 220.63 km^2. Due to the differences between SRTM DEM and Google Earth elevation data, the water level of the Salt Lake simulated would be 12 m or 9.6 m higher than the current level when the lake overflowed, and its reservoir capacity would increase by 23.71 km^3 or 17.27 km^3, respectively. Meanwhile, the overflowed water of the Salt Lake would run into the Qingshui River basin from its eastern part. Although the Salt Lake does not overflow in the coming decade, with watershed expansion of the Salt Lake and the projected precipitation increase in Hoh Xil region, the probability of water overflow from the Salt Lake and becoming a tributary of the Yangtze River will exist in the long term.展开更多
文摘The Qaidam Basin in the NE Tibetan Plateau has contributed the largest amount of potash in China.However,how the potash was formed has long been a subject of debate.Here we carried out a deep drilling
文摘为获得藏灵菇马克斯克鲁维酵母M3菌株胆盐水解酶(BSH)的纯品,探讨了BSH粗品的分离纯化方法。粗酶液采用硫酸铵分级沉淀,再以DEAE-Sepharose Fast Flow离子交换介质,分别考察缓冲液pH值、流速和洗脱方式等对BSH分离纯化的影响。结果表明,在40%～70%饱和度的硫酸铵条件下BSH提取效率最高。最佳层析条件为采用pH值为6.5、50mmol/L磷酸钠缓冲体系、1.5mL/min的流速,进行分步洗脱(100mmol/L、350mmol/L、500mmol/L～600mmol/L NaCl及50mmol/L磷酸盐缓冲液3步洗脱)。在优化纯化条件下BSH的比活力可达479.55AU/mg,是原粗酶液的23.66倍。
基金National Natural Science Foundation of China,No.41261016,No.41561016Opening Foundation Projection of State Key Laboratory of Cryosphere Sciences,CAS,No.SKLCS-OP-2016-10Youth Scholar Scientific Capability Promoting Project of Northwest Normal University,No.NWNU-LKQN-14-4
文摘After the bursting of Huiten Nor in Hoh Xil Region in September, 2011, the topic on whether the water overflowed from the Salt Lake would enter into the Chumaer River and become the northernmost source of the Yangtze River has aroused wide concern from public and academic field. Based on Landsat TM/ETM+/OLI remote sensing images during 2010–2015, SRTM 1 arc-second data, Google Earth elevation data and the observation data from the Wudaoliang meteorological station, the study initially analyzed the variations of the Salt Lake and its overflowing condition and probability. The results showed that the area of the Salt Lake expanded sharply from October 2011 to April 2013, and then it stepped into a stable expansion period. On October 27, 2015, the area of the Salt Lake had arrived at 151.38 km^2, which was about 3.35 times the area of the lake on March 3, 2010. The Salt Lake will overflow when its area reaches the range from 218.90 km^2 to 220.63 km^2. Due to the differences between SRTM DEM and Google Earth elevation data, the water level of the Salt Lake simulated would be 12 m or 9.6 m higher than the current level when the lake overflowed, and its reservoir capacity would increase by 23.71 km^3 or 17.27 km^3, respectively. Meanwhile, the overflowed water of the Salt Lake would run into the Qingshui River basin from its eastern part. Although the Salt Lake does not overflow in the coming decade, with watershed expansion of the Salt Lake and the projected precipitation increase in Hoh Xil region, the probability of water overflow from the Salt Lake and becoming a tributary of the Yangtze River will exist in the long term.
