Objective:To screen the blood group system genes of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province and provide laboratory data for the rare blood group database in this ar...Objective:To screen the blood group system genes of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province and provide laboratory data for the rare blood group database in this area.Methods:The alleles of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of 300 voluntary participants of Li ethnic group in Hainan were detected by sequence-specific primer polymerase chain reaction,and the polymorphism was analyzed.Results:The allele frequencies of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic groups in Hainan Province are 0.9583 for Fy^(a),0.0417 for Fy^(b),0.8350 for Au^(a),0.1650 for Au^(b),0.4500 for Jk^(a),0.5500 for Jk^(b),0.0667 for Di^(a),0.9333 for Di^(b),0.1017 for Doa and 0.8983 for Dob,respectively.The antigen incompatibility rates of Fy^(a)/Fy^(b),Au^(a)/Au^(b),Jk^(a)/Jk^(b),Di^(a)/Di^(b),Doa/Dob of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems were 7.67%,23.76%,37.25%,11.67%and 16.60%,respectively.Conclusion:The gene frequencies of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province are polymorphic,and the antigen incompatibility rates of alleles are higher,which is quite different from that of other nationalities in China and with unique ethnic distribution characteristics.It is of great significance to establish the rare blood group database in this region.展开更多
目的探讨25例B亚型/AB亚型标本的分子生物学机制。方法应用PCR-SSP对血型血清学确认为B抗原减弱的标本进行ABO*B基因检测或应用Sanger一代测序技术对ABO基因的第6-7外显子或第1-7外显子测序;对最终无法定型的标本进一步用PacBio:SMRT三...目的探讨25例B亚型/AB亚型标本的分子生物学机制。方法应用PCR-SSP对血型血清学确认为B抗原减弱的标本进行ABO*B基因检测或应用Sanger一代测序技术对ABO基因的第6-7外显子或第1-7外显子测序;对最终无法定型的标本进一步用PacBio:SMRT三代测序技术进行检测。结果25例B亚型/AB亚型标本均经过基因序列分析,共发现7种已知的ABO*B等位基因,分别为ABO*BW.27(3/25,12.00%)、ABO*BW.03(5/25,20.00%)、ABO*B3.03(2/25,8.00%)、ABO*BEL.03(1/25,4.00%)、ABO*BW.07(7/25,28.00%)、ABO*B3.05(1/25,4.00%)、ABO*BW.12(1/25,4.00%);5种未知的ABO*B等位基因,其中已发表但未被ISBT收录的2例,分别为ABO*B.01 with c.28+5885C>T、ABO*B.01 with c.28+5875C>T,另外3例为本研究首次报道的ABO*B新等位基因,分别为ABO*B.01(with c.3G>C)、ABO*B.01(with c.28+5862A>G)、ABO*B.01(with c.204-3C>G),均已被Genebank收录并公布。结论研究阐释了25例B亚型/AB亚型的分子生物学机制,并发现了5例ABO*B新等位基因。该研究丰富了ABO*B等位基因基因库,为更好地制定输血策略提供了理论依据。展开更多
基金Hainan Provincial Natural Science Foundation of China(No.820QN410)Hainan Province Clinical Medical Center(No.QWYH202175)。
文摘Objective:To screen the blood group system genes of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province and provide laboratory data for the rare blood group database in this area.Methods:The alleles of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of 300 voluntary participants of Li ethnic group in Hainan were detected by sequence-specific primer polymerase chain reaction,and the polymorphism was analyzed.Results:The allele frequencies of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic groups in Hainan Province are 0.9583 for Fy^(a),0.0417 for Fy^(b),0.8350 for Au^(a),0.1650 for Au^(b),0.4500 for Jk^(a),0.5500 for Jk^(b),0.0667 for Di^(a),0.9333 for Di^(b),0.1017 for Doa and 0.8983 for Dob,respectively.The antigen incompatibility rates of Fy^(a)/Fy^(b),Au^(a)/Au^(b),Jk^(a)/Jk^(b),Di^(a)/Di^(b),Doa/Dob of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems were 7.67%,23.76%,37.25%,11.67%and 16.60%,respectively.Conclusion:The gene frequencies of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province are polymorphic,and the antigen incompatibility rates of alleles are higher,which is quite different from that of other nationalities in China and with unique ethnic distribution characteristics.It is of great significance to establish the rare blood group database in this region.
文摘目的探讨25例B亚型/AB亚型标本的分子生物学机制。方法应用PCR-SSP对血型血清学确认为B抗原减弱的标本进行ABO*B基因检测或应用Sanger一代测序技术对ABO基因的第6-7外显子或第1-7外显子测序;对最终无法定型的标本进一步用PacBio:SMRT三代测序技术进行检测。结果25例B亚型/AB亚型标本均经过基因序列分析,共发现7种已知的ABO*B等位基因,分别为ABO*BW.27(3/25,12.00%)、ABO*BW.03(5/25,20.00%)、ABO*B3.03(2/25,8.00%)、ABO*BEL.03(1/25,4.00%)、ABO*BW.07(7/25,28.00%)、ABO*B3.05(1/25,4.00%)、ABO*BW.12(1/25,4.00%);5种未知的ABO*B等位基因,其中已发表但未被ISBT收录的2例,分别为ABO*B.01 with c.28+5885C>T、ABO*B.01 with c.28+5875C>T,另外3例为本研究首次报道的ABO*B新等位基因,分别为ABO*B.01(with c.3G>C)、ABO*B.01(with c.28+5862A>G)、ABO*B.01(with c.204-3C>G),均已被Genebank收录并公布。结论研究阐释了25例B亚型/AB亚型的分子生物学机制,并发现了5例ABO*B新等位基因。该研究丰富了ABO*B等位基因基因库,为更好地制定输血策略提供了理论依据。
